Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,580,9820TC59.0% 302.5 / inf 469V209A (GTT→GCT) adhEbifunctional acetaldehyde‑CoA/alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (86/105);  new base C (124/151);  total (211/258)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01

Only 100 of 474 total aligned reads displayed.

CTGCCGGTGCTCCGAAAGATCTGATCGGCTGGATCGATCAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAGTCTGTTGTTGTTGTTGACTCTGTTT  >  DH1/2580832‑2581131
                                                                                                                                                      |                                                                                                                                                     
    gggTGCTCCGAAATATCTGATCGGCTGGATCGATCAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATc                                                                                                                                                   <  7:652905/150‑1 (MQ=255)
    cagTGCTCCGAAAGATCTGATAGCCTGGATCCATCAACCTTCTGTTGAACTGTCTAACGCACTCAAGCACCACCCAGACATCAACCTCAACCTCGCAACTGGACGACCGGGCATGCTTAAACCACCATACAGATACGGTAAACAAGTTATc                                                                                                                                                   >  2:2131826/3‑151 (MQ=255)
     ggTGCTCCGAAAGATCTGATCGGCTGGATCGATCAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCg                                                                                                                                                  <  5:643435/151‑1 (MQ=255)
        gCTCCGAAAGATCTGATCGGCTGGATCGATCAACCTTCTGTTGAAGTGTCTAACGCACTGATGCACCACCCACACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGgt                                                                                                                                                <  8:1219171/150‑1 (MQ=255)
        gCTCCGAAAGATCTGATCGGCTGGATCGAGCAACCTTCTGTGGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGgtg                                                                                                                                               <  8:357809/151‑1 (MQ=255)
         cTCCGAAAGATCTGATCGGCTGGATCGATCAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGCCATCACCCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGgtgt                                                                                                                                              >  6:344899/1‑151 (MQ=255)
            cGAAAGATCTGATCGGCTGGATCGATCAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAgg                                                                                                                                           <  4:1388678/151‑1 (MQ=255)
                 gATCTGATCGGCTGGATCGATCAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGcgcg                                                                                                                                       <  2:97351/150‑1 (MQ=255)
                     tGATCGGCTGGATCGATCAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCa                                                                                                                                   >  2:1935966/1‑150 (MQ=255)
                      gATCGGCTGGATCGATCAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAac                                                                                                                                 >  5:2026346/1‑151 (MQ=255)
                              ggATCGATCAACCTTGTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCAACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAgt                                                                                                                         <  8:691338/151‑1 (MQ=255)
                              ggATCGATCAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAg                                                                                                                          <  5:472409/150‑1 (MQ=255)
                               gatcgatcAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAgtt                                                                                                                        <  1:909115/151‑1 (MQ=255)
                                atcgatcAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAgttt                                                                                                                       >  2:1789059/1‑150 (MQ=255)
                                  cgatcAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAgttgtt                                                                                                                     >  4:151315/1‑151 (MQ=255)
                                  cgatcAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAgttgtt                                                                                                                     <  4:1004894/151‑1 (MQ=255)
                                    atcAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTAt                                                                                                                   <  4:524458/151‑1 (MQ=255)
                                    atcAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTAt                                                                                                                   >  4:2019713/1‑151 (MQ=255)
                                    atcAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTCTAGGCGCGGGCAACACTCCAGTTGTTAt                                                                                                                   >  2:471295/1‑151 (MQ=255)
                                        acgttgtGTTGAACTGTCTAACGCACTGAGCCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGAt                                                                                                               <  7:1908427/145‑1 (MQ=255)
                                        aCCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGAt                                                                                                               <  2:1518589/151‑1 (MQ=255)
                                        aCCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGAt                                                                                                               <  3:1577015/151‑1 (MQ=255)
                                         ccTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATg                                                                                                              <  1:1524773/151‑1 (MQ=255)
                                         ccTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATg                                                                                                              >  1:1765267/1‑151 (MQ=255)
                                            tCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGa                                                                                                             <  2:570864/149‑1 (MQ=255)
                                             cTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAAc                                                                                                          >  1:546385/1‑151 (MQ=255)
                                               gTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAAct                                                                                                         <  2:2052117/150‑1 (MQ=255)
                                               gTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAAct                                                                                                         <  5:1396724/150‑1 (MQ=255)
                                                  gAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAActgctg                                                                                                     >  2:1219846/1‑151 (MQ=255)
                                                   aaCTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAActgctg                                                                                                     >  7:1058748/1‑150 (MQ=255)
                                                    agtGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTATAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGat                                                                                                   <  5:1683393/149‑1 (MQ=255)
                                                    aCTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGat                                                                                                   <  8:1488364/151‑1 (MQ=255)
                                                    aCTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGat                                                                                                   >  8:768524/1‑151 (MQ=255)
                                                     cTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGat                                                                                                   >  2:672288/1‑150 (MQ=255)
                                                     cTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGat                                                                                                   >  3:85439/1‑150 (MQ=255)
                                                     atGTCTAACGCACTGATGCACCACCCACACATCACCCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGata                                                                                                  <  1:1096457/150‑1 (MQ=255)
                                                       gTCTAACGCACTGATGCACCAGCCAGACATGAACGTGATCCTGGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATTCAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGatat                                                                                                 <  2:1017816/150‑1 (MQ=255)
                                                         atAACGAACTGATGCACCACCCAGACTTCAACCTGCTCGTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCaa                                                                                              <  7:815766/150‑1 (MQ=255)
                                                          tAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCaa                                                                                              >  4:1906380/1‑150 (MQ=255)
                                                           aaCGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCaaa                                                                                             <  7:1336966/150‑1 (MQ=255)
                                                              gCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTg                                                                                         >  4:283328/1‑151 (MQ=255)
                                                                aCTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGc                                                                                        >  1:445810/1‑150 (MQ=255)
                                                                   gATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGtt                                                                                    <  6:825182/151‑1 (MQ=255)
                                                                    aTGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTg                                                                                   <  4:229147/151‑1 (MQ=255)
                                                                        accaccCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATc                                                                               <  5:793026/151‑1 (MQ=255)
                                                                        accaccCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATc                                                                               >  4:1648197/1‑151 (MQ=255)
                                                                        accaccCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATc                                                                               >  8:2022207/1‑151 (MQ=255)
                                                                         ccaccCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCt                                                                              <  3:1841249/151‑1 (MQ=255)
                                                                            cccAGACATCAAGCTGATCCGCGCTAGTGGTGGTGCGGGCATGGTTAATGCCGCATACAGCTGCGGTAAACCTGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTa                                                                           <  4:714416/151‑1 (MQ=255)
                                                                              cAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACt                                                                         <  1:1933057/151‑1 (MQ=255)
                                                                                 aCATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGAt                                                                      <  5:563043/151‑1 (MQ=255)
                                                                                 aCATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGAt                                                                      <  7:1850777/151‑1 (MQ=255)
                                                                                  cATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATg                                                                     <  4:387343/151‑1 (MQ=255)
                                                                                        ccTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCaaa                                                               <  5:1511571/151‑1 (MQ=255)
                                                                                         cTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCaaa                                                               >  6:682825/1‑150 (MQ=255)
                                                                                           gATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAAcc                                                            <  7:2121217/151‑1 (MQ=255)
                                                                                           gATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAAcc                                                            <  1:635242/151‑1 (MQ=255)
                                                                                           gATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCaaaa                                                              <  4:1826881/149‑1 (MQ=255)
                                                                                           gATCCTCGCGACTGGGGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCaaaa                                                              <  3:211818/149‑1 (MQ=255)
                                                                                             tCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCtt                                                          >  8:51010/1‑151 (MQ=255)
                                                                                              ccTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCtt                                                          <  1:30201/150‑1 (MQ=255)
                                                                                              ccTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTc                                                         <  4:927741/151‑1 (MQ=255)
                                                                                              ccTCGCGACTGGTGGGCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCtt                                                          <  7:229766/150‑1 (MQ=255)
                                                                                              ccTCGCGACTGGTGGACCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTc                                                         <  7:1416665/151‑1 (MQ=255)
                                                                                                 cgcgACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGa                                                       <  2:1635221/150‑1 (MQ=255)
                                                                                                 cgcgACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGa                                                       <  7:1657470/150‑1 (MQ=255)
                                                                                                 cgcgACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGAc                                                      >  8:61010/1‑151 (MQ=255)
                                                                                                   cgACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACa                                                     >  8:1636097/1‑150 (MQ=255)
                                                                                                   cgACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACaa                                                    <  5:178545/151‑1 (MQ=255)
                                                                                                    gACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAAc                                                   <  4:116596/151‑1 (MQ=255)
                                                                                                     aCTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACg                                                  >  5:261574/1‑151 (MQ=255)
                                                                                                         gtggtCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGt                                              <  6:2024590/151‑1 (MQ=255)
                                                                                                           ggtCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTa                                             >  5:1225258/1‑150 (MQ=255)
                                                                                                                gggCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCtgt                                       <  6:1760215/151‑1 (MQ=255)
                                                                                                                 ggCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCtgtg                                      >  6:953336/1‑151 (MQ=255)
                                                                                                                  gCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGc                                     >  7:1717288/1‑151 (MQ=255)
                                                                                                                   cATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCt                                    >  8:2117209/1‑151 (MQ=255)
                                                                                                                       gTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTg                                <  7:1238744/151‑1 (MQ=255)
                                                                                                                       gTTAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTg                                >  3:1914183/1‑151 (MQ=255)
                                                                                                                         tAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGa                               >  3:108645/1‑150 (MQ=255)
                                                                                                                         tAAAGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGa                               <  7:947688/150‑1 (MQ=255)
                                                                                                                          aaaGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAAc                             >  8:1394115/1‑151 (MQ=255)
                                                                                                                           aaGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACa                            <  6:1098986/151‑1 (MQ=255)
                                                                                                                           aaGCCGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACa                            <  6:547646/151‑1 (MQ=255)
                                                                                                                            aGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAg                           <  3:14727/151‑1 (MQ=255)
                                                                                                                              ccGCATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGAGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAGTc                         >  2:776858/1‑151 (MQ=255)
                                                                                                                               cGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAGTCt                        <  6:1856299/151‑1 (MQ=255)
                                                                                                                                 cATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAGTCtgt                      <  5:2149215/151‑1 (MQ=255)
                                                                                                                                 cATACAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAGTCtgt                      >  5:354684/1‑151 (MQ=255)
                                                                                                                                  aTACAGCTCCGATAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAGTCtgtt                     <  1:1011826/151‑1 (MQ=255)
                                                                                                                                     cAGCTCGGTTAAACCAGTTATCGGTGTAGGAGCGGGCAAGACTCCTGTTGTTATGGATGAATCTGCTGATATCAAACGTGCAGTTGAATCTGTACTGATGTCCAAAACCTTCGAGAACGGCGTAATCTGTGCTTCTGAACAGTCtgttgtt                  <  1:2132114/151‑1 (MQ=255)
                                                                                                                                     cAGCTCCGGTAAACCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAGTCtgttgtt                  <  3:473675/151‑1 (MQ=255)
                                                                                                                                          ccGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAGTCtgttgttgtt               <  6:1503419/149‑1 (MQ=255)
                                                                                                                                           cGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAGTCTgttgttgttgtt            >  7:563074/1‑151 (MQ=255)
                                                                                                                                              tAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAGTCTGTTGTTGTTGTTGAc         >  3:517147/1‑151 (MQ=255)
                                                                                                                                               aaaCCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCTAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAGTCTGTTGTTGTTGTTGAct        <  1:845221/151‑1 (MQ=255)
                                                                                                                                               aaaCCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAGTCTGTTGTTGTTGTTGAct        >  1:1178489/1‑151 (MQ=255)
                                                                                                                                                 aCCAGCTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAGTCTGTTGTTGTTGTTGActct      <  8:954130/151‑1 (MQ=255)
                                                                                                                                                     gTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATAGCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAGTCTGTTGTTGTTGTTGACTCTGttt  <  3:1212977/151‑1 (MQ=255)
                                                                                                                                                      ttATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAGTCTGTTGTTGTTGTTGACTCTGttt  >  5:193855/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CTGCCGGTGCTCCGAAAGATCTGATCGGCTGGATCGATCAACCTTCTGTTGAACTGTCTAACGCACTGATGCACCACCCAGACATCAACCTGATCCTCGCGACTGGTGGTCCGGGCATGGTTAAAGCCGCATACAGCTCCGGTAAACCAGTTATCGGTGTAGGCGCGGGCAACACTCCAGTTGTTATCGATGAAACTGCTGATATCAAACGTGCAGTTGCATCTGTACTGATGTCCAAAACCTTCGACAACGGCGTAATCTGTGCTTCTGAACAGTCTGTTGTTGTTGTTGACTCTGTTT  >  DH1/2580832‑2581131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: