Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,688,0950AG58.8% 319.8 / inf 502K115E (AAA→GAA) purBadenylosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base A (100/107);  new base G (127/168);  total (227/275)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-03

Only 100 of 508 total aligned reads displayed.

GTTACCTTGATGCAATCGTCGCCAGTTTCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCGTTCAGTATCGCGATATC  >  DH1/2687946‑2688244
                                                                                                                                                     |                                                                                                                                                     
   aCCTTGATGCAATCGTCGCCCGTTGCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGTGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAAt                                                                                                                                                    <  6:1947089/150‑1 (MQ=255)
   aCCTTGATGCAATCGTCGCCAGTTTCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAAtt                                                                                                                                                   >  6:853177/1‑151 (MQ=255)
    ccTTGATGCAATCGTCGCCAGTTTCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTc                                                                                                                                                  <  7:1981805/151‑1 (MQ=255)
       tGATGCAATCTTAGCCAGTTTCAGCGAAGAAGATGCGGCTCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCa                                                                                                                                                 <  3:804119/149‑1 (MQ=255)
       tGATGCAATCGTCGCCAGTTTCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATc                                                                                                                                               >  8:2205864/1‑151 (MQ=255)
        gATGCAATCGTCGCCAGTTTCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATcc                                                                                                                                              <  3:1165268/151‑1 (MQ=255)
         aTGCAATCGTCGCCAGTTTCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATcc                                                                                                                                              <  5:1186027/150‑1 (MQ=255)
         aTGCAATCGTCGCCAGTTTCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCa                                                                                                                                             <  6:1079067/151‑1 (MQ=255)
          tgcattgttctccAGTTTCAGCGAAGAAGATGCGGCGCGCATCAAAACTTTGGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGATAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCa                                                                                                                                             <  7:1628275/139‑1 (MQ=255)
           gCAATCGTCGCCAGTTTCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACt                                                                                                                                           <  8:1331636/151‑1 (MQ=255)
           gCAATCGTCGCCAGTTTCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACt                                                                                                                                           >  7:917428/1‑151 (MQ=255)
           gCAATCGTCGCCAGTTTCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACt                                                                                                                                           <  1:1330609/151‑1 (MQ=255)
            cAATCGTCGCCAGTTTCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACtt                                                                                                                                          <  1:632975/151‑1 (MQ=255)
             aaTCGTCGCCAGTTTCAGCTAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTACCCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACttt                                                                                                                                         <  5:293804/151‑1 (MQ=255)
              aTCGTGGCCAGTTTCAGCTATGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTg                                                                                                                                        <  3:1697184/151‑1 (MQ=255)
                    gCCAGTTTCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCTTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTa                                                                                                                                  <  1:1958440/151‑1 (MQ=255)
                     ccAGTTTCAGCGAAGAAGATGGGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTAc                                                                                                                                 <  1:1018150/151‑1 (MQ=255)
                        gTTTCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACt                                                                                                                                <  2:1676606/149‑1 (MQ=255)
                        gTTTCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTc                                                                                                                              >  8:1445258/1‑151 (MQ=255)
                         tttCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTc                                                                                                                              <  5:1834171/150‑1 (MQ=255)
                          ttCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCg                                                                                                                             <  2:1996248/150‑1 (MQ=255)
                          ttCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCg                                                                                                                             <  6:207991/150‑1 (MQ=255)
                          ttCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCgg                                                                                                                            >  5:920754/1‑151 (MQ=255)
                           tCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGGTTCTAAATTCATCCACTTTGCCTGTACTTCgg                                                                                                                            <  7:1033156/150‑1 (MQ=255)
                            cAGGGAAGAAGATCCGGCGGGCATCAAAAGTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCAGTTTGCCTGTACTTCGGaa                                                                                                                          <  4:1814680/151‑1 (MQ=255)
                             aGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAg                                                                                                                         <  2:840373/151‑1 (MQ=255)
                               cGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGat                                                                                                                       <  5:764540/151‑1 (MQ=255)
                                       aTGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATaa                                                                                                               <  3:1982027/151‑1 (MQ=255)
                                       aTGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATa                                                                                                                >  5:1986911/1‑150 (MQ=255)
                                       aTGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATaa                                                                                                               <  5:1625771/151‑1 (MQ=255)
                                        tGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATaa                                                                                                               <  4:1289354/150‑1 (MQ=255)
                                        tGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAAc                                                                                                              >  6:1257639/1‑151 (MQ=255)
                                          cggcgCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACct                                                                                                            <  8:357553/151‑1 (MQ=255)
                                            gcgcgcATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACctct                                                                                                          <  3:1857007/151‑1 (MQ=255)
                                                gcATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCa                                                                                                      <  7:2197927/151‑1 (MQ=255)
                                                 cATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCAc                                                                                                     >  7:437822/1‑151 (MQ=255)
                                                  aTCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACg                                                                                                    <  3:2097823/151‑1 (MQ=255)
                                                  aTCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACg                                                                                                    <  4:1681092/151‑1 (MQ=255)
                                                     aaaaCTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCAt                                                                                                 <  8:1115939/151‑1 (MQ=255)
                                                      aaaCTATCGAGCGTACCACTAACCCCGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATACCCTCTCCCCCGCAtt                                                                                                >  8:531928/1‑151 (MQ=255)
                                                      aaaCTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCAtt                                                                                                <  3:1581797/151‑1 (MQ=255)
                                                         atATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAAt                                                                                             <  1:1674001/150‑1 (MQ=255)
                                                          tATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATCACCTCTCCCACGCATTAATg                                                                                            >  8:175627/1‑151 (MQ=255)
                                                           aTCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGc                                                                                           <  7:625073/151‑1 (MQ=255)
                                                             cGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCt                                                                                          <  7:271082/150‑1 (MQ=255)
                                                              gAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTg                                                                                         >  6:1431427/1‑150 (MQ=255)
                                                              gAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTg                                                                                         >  5:227158/1‑150 (MQ=255)
                                                               aGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGaa                                                                                       <  2:1930058/151‑1 (MQ=255)
                                                                gCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGaaa                                                                                      <  4:911622/151‑1 (MQ=255)
                                                                  gTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAAc                                                                                    <  7:1507882/151‑1 (MQ=255)
                                                                    aCCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAAcc                                                                                   <  8:696188/150‑1 (MQ=255)
                                                                    aCCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACcg                                                                                  <  2:716873/151‑1 (MQ=255)
                                                                       aCTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACcgcgc                                                                               <  5:1064170/151‑1 (MQ=255)
                                                                          aaCCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACcgcgcg                                                                              <  4:491386/149‑1 (MQ=255)
                                                                          aaCCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGtg                                                                            <  6:673512/151‑1 (MQ=255)
                                                                            ccACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGtgat                                                                          >  2:1031813/1‑151 (MQ=255)
                                                                             cACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGtgatg                                                                         <  8:1205782/151‑1 (MQ=255)
                                                                              acgacgTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGtgatga                                                                        <  7:745638/151‑1 (MQ=255)
                                                                                gacgTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAg                                                                      <  5:2133971/151‑1 (MQ=255)
                                                                                  cgTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTg                                                                    >  1:2218846/1‑151 (MQ=255)
                                                                                   gTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTg                                                                    >  3:1300936/1‑150 (MQ=255)
                                                                                    ttAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGAt                                                                  >  3:683305/1‑151 (MQ=255)
                                                                                     tAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCTGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGAt                                                                  <  5:617452/150‑1 (MQ=255)
                                                                                     tAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGAt                                                                  <  2:1748832/150‑1 (MQ=255)
                                                                                            gTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCAt                                                          >  7:2114761/1‑151 (MQ=255)
                                                                                             ttGAGTATTTCCTGAAAGATAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAAGCGCGCGTGATGAAGTGATCCTGCCAt                                                          >  2:1233950/1‑150 (MQ=255)
                                                                                                   aTTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGc                                                    <  7:818573/150‑1 (MQ=255)
                                                                                                   aTTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCg                                                   >  6:234348/1‑151 (MQ=255)
                                                                                                    tttCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGt                                                  >  3:1498003/1‑151 (MQ=255)
                                                                                                      tCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTc                                                 >  4:1063491/1‑150 (MQ=255)
                                                                                                      tCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTc                                                 <  5:854194/150‑1 (MQ=255)
                                                                                                       ccTGAAACAAAAAGTGCCGGAGATCCCGGAACTGCACCCCGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTCCCATACTGGCGTCac                                               >  4:1902480/1‑150 (MQ=255)
                                                                                                           aaagaaaAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACtga                                           <  4:2111279/151‑1 (MQ=255)
                                                                                                                 aaaGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATg                                     >  3:346593/1‑151 (MQ=255)
                                                                                                                         ggAGATCCCGTAAATGCCCGCGTTTGCTGAATTCATCCACTTTGCCTGTACTTCGGAATATATCCATAACCTCTCCCACGCATTAATGCTTAAAACCGCGCGTGATTAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTaaa                             <  1:11044/151‑1 (MQ=255)
                                                                                                                         ggAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTaan                             <  4:1519256/151‑2 (MQ=255)
                                                                                                                         ggAGATCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTaaa                             <  5:444788/151‑1 (MQ=255)
                                                                                                                         ggAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTaaa                             <  6:431740/151‑1 (MQ=255)
                                                                                                                         ggAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTaaa                             <  4:782553/151‑1 (MQ=255)
                                                                                                                          gagaTCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTaa                              >  3:505778/1‑149 (MQ=255)
                                                                                                                          gagaTCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAg                            <  4:1376583/151‑1 (MQ=255)
                                                                                                                           agaTCCCGGAACTGCACGCGGTTTCTAATTTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCAACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTaa                              >  7:1327450/1‑148 (MQ=255)
                                                                                                                             aTCCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGAtc                         <  4:284877/151‑1 (MQ=255)
                                                                                                                               cccGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGAtctc                       >  6:1505037/1‑151 (MQ=255)
                                                                                                                                ccGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCg                      <  2:603010/151‑1 (MQ=255)
                                                                                                                                 cGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGc                     <  8:1959482/151‑1 (MQ=255)
                                                                                                                                   gAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCg                   <  4:1147162/151‑1 (MQ=255)
                                                                                                                                       tGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCGTTc                >  6:1661919/1‑150 (MQ=255)
                                                                                                                                        gCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCGTTc                <  6:548770/149‑1 (MQ=255)
                                                                                                                                         cACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCGTTCAGt             <  8:838557/151‑1 (MQ=255)
                                                                                                                                         cACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCGTTCAGt             <  1:1875799/151‑1 (MQ=255)
                                                                                                                                          aCGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCGTTCAGTa            <  3:227189/151‑1 (MQ=255)
                                                                                                                                            gcgGTTTCTGAATTCATCGACTTTGACTGTACTTCGGAAGATATCAATAACCTCTCCCACGAATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCGTTCAGTATc          <  2:385837/151‑1 (MQ=255)
                                                                                                                                            gcgGTTTCTAAATTCATCCACTTTGGCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGTCATTAAAGATCTCGCCGTTCAGTATc          <  1:916861/151‑1 (MQ=255)
                                                                                                                                            gcgGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCGTTCAGTATc          <  1:2097974/151‑1 (MQ=255)
                                                                                                                                             cgGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCGTTCAGTATcg         >  1:392704/1‑151 (MQ=255)
                                                                                                                                               gTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCGTTCAGTATcgcg       <  5:1433082/151‑1 (MQ=255)
                                                                                                                                               gTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCGTTCAGTATcgcg       >  2:958910/1‑151 (MQ=255)
                                                                                                                                               gTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCGTTCAGTATcgcg       >  7:1795145/1‑151 (MQ=255)
                                                                                                                                                  tCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCGTTCAGTATCGCGat     <  8:1836594/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTACCTTGATGCAATCGTCGCCAGTTTCAGCGAAGAAGATGCGGCGCGCATCAAAACTATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGATCCCGGAACTGCACGCGGTTTCTAAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAATGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCATACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCGTTCAGTATCGCGATATC  >  DH1/2687946‑2688244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑