Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,709,9030CT59.2% 387.5 / inf 539P179L (CCG→CTG) ldtCpeptidoglycan‑binding protein LysM
Reads supporting (aligned to +/- strand):  ref base C (100/120);  new base T (156/163);  total (256/283)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 545 total aligned reads displayed.

TGGTCAGTTAGGTGGTGACACGCTGACACCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGCGGGATGACGATATCAAAACACTCTTTA  >  DH1/2709754‑2710052
                                                                                                                                                     |                                                                                                                                                     
  gTCAGTTAGGTGGTGACACGCTGACACCGACATTGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGGCCGTTATAAAGCAGAGCCAATTGAGTAGCCTGCGGTAGTGCCGGCTGGACTgg                                                                                                                                                     >  2:538107/1‑150 (MQ=255)
  gTCAGTTAGGTGGTGACACGCTGACACCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTgg                                                                                                                                                     >  1:2039757/1‑150 (MQ=255)
    cAGTTAGGTGGTGACACGCTGACACCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGAt                                                                                                                                                   >  6:813186/1‑150 (MQ=255)
    cAGTTAGGTGGTGACACGCTGACACCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATa                                                                                                                                                  <  2:107/151‑1 (MQ=255)
     aGTTAGGTGGTGACACGCTGACACCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATa                                                                                                                                                  >  8:650109/1‑150 (MQ=255)
        tAGGTGGTGACACGCTGACACCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAAccc                                                                                                                                              >  7:747228/1‑151 (MQ=255)
             ggtgACACGCTGACACCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATg                                                                                                                                          <  8:754523/150‑1 (MQ=255)
                  cacGCTGACACCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCcat                                                                                                                                    >  6:960082/1‑151 (MQ=255)
                   acGCTGACACCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCcatc                                                                                                                                   <  4:177004/151‑1 (MQ=255)
                       tGACACCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGTAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGc                                                                                                                               >  1:18505/1‑151 (MQ=255)
                       tGACACCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGc                                                                                                                               >  6:578992/1‑151 (MQ=255)
                        gACACCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGc                                                                                                                               <  1:859209/150‑1 (MQ=255)
                        gACACCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCg                                                                                                                              >  2:166801/1‑151 (MQ=255)
                        gACACCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATg                                                                                                                                <  6:1551081/149‑1 (MQ=255)
                        gACACCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGc                                                                                                                               <  2:1901062/150‑1 (MQ=255)
                         acacCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGCCGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCg                                                                                                                              >  3:533183/1‑150 (MQ=255)
                          cacCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGAt                                                                                                                            <  8:2172384/151‑1 (MQ=255)
                           acCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGAtt                                                                                                                           >  5:303795/1‑151 (MQ=255)
                               aCAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTc                                                                                                                       <  3:1062848/151‑1 (MQ=255)
                                 aaTGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTg                                                                                                                     <  6:2148692/151‑1 (MQ=255)
                                  aTGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTgg                                                                                                                    >  6:1424131/1‑151 (MQ=255)
                                  aTGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTgg                                                                                                                    <  1:748555/151‑1 (MQ=255)
                                    ggTTACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTggc                                                                                                                   <  7:1979761/150‑1 (MQ=255)
                                     gTGACCAGGGTTTCAGACTATCGTGCACACCCAACCTGGACGCAAACGGCAAACATCCGCGCCNGTTATAACGGACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGTCTGGATAACCCAATGGGCCATCATGCGATTCGTCTggagg                                                                                                                 <  4:1374199/151‑1 (MQ=255)
                                       gACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGcc                                                                                                               >  4:1042103/1‑151 (MQ=255)
                                       gACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGcc                                                                                                               >  7:1128187/1‑151 (MQ=255)
                                          caccGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTAt                                                                                                            >  1:1312246/1‑151 (MQ=255)
                                            ccGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATgg                                                                                                          <  7:2117279/151‑1 (MQ=255)
                                             cGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATggc                                                                                                         <  4:1617895/151‑1 (MQ=255)
                                               tttCAGCCAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATACCCCAATGGGCCATCCTGCGAGGCGTCTGGCGGCCTATggcgg                                                                                                       >  6:333031/1‑151 (MQ=255)
                                                  cAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGt                                                                                                    <  6:1204403/151‑1 (MQ=255)
                                                     aCAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTa                                                                                                 <  1:590009/151‑1 (MQ=255)
                                                       aaaCGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTAtt                                                                                               <  5:2070790/151‑1 (MQ=255)
                                                          cGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTgg                                                                                            >  3:410045/1‑150 (MQ=255)
                                                             gCAAACCCAACGTGGACGCCTACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTc                                                                                         <  8:1327345/151‑1 (MQ=255)
                                                             gCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTc                                                                                         <  3:1343918/151‑1 (MQ=255)
                                                             gCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCtt                                                                                          <  1:1385807/150‑1 (MQ=255)
                                                              cAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTc                                                                                         >  2:876402/1‑150 (MQ=255)
                                                              cAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCa                                                                                        <  8:1197240/151‑1 (MQ=255)
                                                               aaaCCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCAt                                                                                       >  5:1349988/1‑151 (MQ=255)
                                                                   ccAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTa                                                                                   <  1:1599650/151‑1 (MQ=255)
                                                                    cAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTAc                                                                                  <  7:456883/151‑1 (MQ=255)
                                                                     aaCCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACg                                                                                 >  7:220292/1‑151 (MQ=255)
                                                                     aaCCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACg                                                                                 >  7:1790961/1‑151 (MQ=255)
                                                                           gACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGcc                                                                           <  7:1464752/151‑1 (MQ=255)
                                                                           gACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGcc                                                                           <  5:2202133/151‑1 (MQ=255)
                                                                           gACGCCAACGGCAAACATCCGCGCCCATTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGcc                                                                           >  6:1343964/1‑151 (MQ=255)
                                                                            aCGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCg                                                                          <  5:1931118/151‑1 (MQ=255)
                                                                              gCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGAt                                                                        >  5:431600/1‑151 (MQ=255)
                                                                               ccAACGGCAAACATCCGCGCCCGTTATAAATAACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATAATTCGATTCGTCTGGCGGACTATGGCGGCGTTTATTTGCGTAATGGTACGAACGCCGAtt                                                                       >  6:1887827/1‑151 (MQ=255)
                                                                               ccAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGAtt                                                                       <  1:505760/151‑1 (MQ=255)
                                                                                cAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGAttt                                                                      <  5:579775/151‑1 (MQ=255)
                                                                                 aaCGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGGACGAACGCCGAtttg                                                                     >  4:733590/1‑150 (MQ=255)
                                                                                 aaCGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTc                                                                     <  7:1251872/151‑1 (MQ=255)
                                                                                  aCGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCg                                                                    <  6:1093057/151‑1 (MQ=255)
                                                                                        aaCATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTg                                                              <  3:1806870/151‑1 (MQ=255)
                                                                                          cATCCGCGCGCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGc                                                            <  8:272866/151‑1 (MQ=255)
                                                                                            tCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCAt                                                          >  2:153713/1‑151 (MQ=255)
                                                                                             ccGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCAt                                                          <  1:1020553/150‑1 (MQ=255)
                                                                                               gcgcCCGTTATAAAGCACAGGGAATTGAGGGGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCg                                                       <  1:239503/151‑1 (MQ=255)
                                                                                                ggcCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCgg                                                      <  4:262716/150‑1 (MQ=255)
                                                                                                cgcCCGTTATAAAGCACAGGGAATTGAGTGGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCgg                                                      <  3:1215776/151‑1 (MQ=255)
                                                                                                 gcCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCggg                                                     <  3:215616/151‑1 (MQ=255)
                                                                                                 gcCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCggg                                                     <  6:1769485/151‑1 (MQ=255)
                                                                                                  cccGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGt                                                    <  3:1943145/151‑1 (MQ=255)
                                                                                                  cccGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATGTCGGCATTGGCATGCGGGt                                                    >  3:1006833/1‑151 (MQ=255)
                                                                                                   ccGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTa                                                   <  7:633601/151‑1 (MQ=255)
                                                                                                   ccGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCGATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTa                                                   <  1:1758907/151‑1 (MQ=255)
                                                                                                     gTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTTTTTGCTTAATGGTACGAACGCCGATTTCGGCATTGGGATGCGGGTa                                                   >  8:1517761/1‑149 (MQ=255)
                                                                                                          aaaGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCt                                             <  4:1193633/150‑1 (MQ=255)
                                                                                                           aaGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTgg                                           >  2:396152/1‑151 (MQ=255)
                                                                                                             gCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCt                                         <  5:1056622/151‑1 (MQ=255)
                                                                                                               acaGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGt                                       <  8:1137065/151‑1 (MQ=255)
                                                                                                                 aGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTAt                                     >  5:1265380/1‑151 (MQ=255)
                                                                                                                 aGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTAt                                     <  5:1769349/151‑1 (MQ=255)
                                                                                                                 aGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTAt                                     <  3:276970/151‑1 (MQ=255)
                                                                                                                  gggAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTAtt                                    <  8:529178/151‑1 (MQ=255)
                                                                                                                  gggAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTAtt                                    <  4:856488/151‑1 (MQ=255)
                                                                                                                   ggAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTc                                   <  8:2001258/151‑1 (MQ=255)
                                                                                                                     aaTTGAGTTGCCTCCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGGTTATTTGCGTCATGGTACGAACCCCGATTTCGGCATTGGCATGCCGGGAAGTTCTGGCTGTATTAGt                                 >  2:1350129/1‑151 (MQ=255)
                                                                                                                      aTTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGt                                 >  5:884127/1‑150 (MQ=255)
                                                                                                                      aTTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTc                                <  2:1280566/151‑1 (MQ=255)
                                                                                                                         gAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGc                             >  4:406146/1‑151 (MQ=255)
                                                                                                                         gAGTTGCCTGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGc                             <  5:1667434/151‑1 (MQ=255)
                                                                                                                         gAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTg                              <  2:750395/150‑1 (MQ=255)
                                                                                                                         gAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTAATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTgg                             >  2:217993/1‑150 (MQ=255)
                                                                                                                          aGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGCg                            >  1:1510422/1‑151 (MQ=255)
                                                                                                                               ccTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGCGGGATg                       >  4:533613/1‑151 (MQ=255)
                                                                                                                                 tGCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGCGGGATGAc                     >  5:55849/1‑151 (MQ=255)
                                                                                                                                 ggCGGTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATACGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGCGGGATGAc                     <  6:699794/150‑1 (MQ=255)
                                                                                                                                    ggTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGCGGGATGACGat                  >  3:784645/1‑151 (MQ=255)
                                                                                                                                    ggTAGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGCGGGATGACGat                  >  2:2026089/1‑151 (MQ=255)
                                                                                                                                    ggTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCAAGGTACGAACGCCGATTTCGGCAATGGCAGGCGGGTAAGTTCTGGGGGTATTCGTGGGCGGGCTGACgct                  >  8:1887131/1‑149 (MQ=255)
                                                                                                                                       aGTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGCGGGATGACGatat                <  3:1512419/150‑1 (MQ=255)
                                                                                                                                       aGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGCGGGATGACGATATc               <  6:423084/151‑1 (MQ=255)
                                                                                                                                        gTGCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGCGGGATGACGATATCa              <  5:1487330/151‑1 (MQ=255)
                                                                                                                                         ggCCGGCTGGACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGCGGGATGACGATATCaa             <  8:1045849/150‑1 (MQ=255)
                                                                                                                                          gCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGCGGGTTGACGATATCaa             >  4:1997840/1‑150 (MQ=255)
                                                                                                                                                 ggACTGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGCGGGATGACGATATCAAAACActct     <  5:721252/151‑1 (MQ=255)
                                                                                                                                                 ggACCGGATAAGCCAATGGGCCATCATGCGATTCGTCTGGCTGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCAGATTTCGGCATTGGCATGCGGGTAAGTTCTGGATGTATTCGTCTGCGGGATGACGATATCAAAACAct       <  3:632168/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGTCAGTTAGGTGGTGACACGCTGACACCGACAATGGTGACCACCGTTTCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAAGCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCATCATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGATTTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGCGGGATGACGATATCAAAACACTCTTTA  >  DH1/2709754‑2710052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑