Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,026,7830AG59.2% 371.0 / inf 568C269R (TGT→CGT) lptFLPS export ABC transporter permease LptF
Reads supporting (aligned to +/- strand):  ref base A (123/107);  new base G (175/159);  total (299/269)
Fisher's exact test for biased strand distribution p-value = 8.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.61e-01

Only 100 of 573 total aligned reads displayed.

CGATTTCAGGGAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCGGAAATCACGTAA  >  DH1/4026634‑4026933
                                                                                                                                                     |                                                                                                                                                      
 gATTTCAGGGAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACAcc                                                                                                                                                      >  4:2193547/1‑151 (MQ=255)
     tCAGGGAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCcagt                                                                                                                                                   <  1:2090632/150‑1 (MQ=255)
     tCAGGGAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACcagtt                                                                                                                                                  >  3:1538612/1‑151 (MQ=255)
      cAGGGAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCcagtt                                                                                                                                                  <  7:1786048/150‑1 (MQ=255)
       aGGGAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACcagttc                                                                                                                                                 >  5:81014/1‑150 (MQ=255)
            ggTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCt                                                                                                                                           >  8:1845726/1‑151 (MQ=255)
            ggTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCt                                                                                                                                           <  1:606112/151‑1 (MQ=255)
             gTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTg                                                                                                                                          <  7:75267/151‑1 (MQ=255)
             gTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTg                                                                                                                                          >  3:1275657/1‑151 (MQ=255)
              tCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTgc                                                                                                                                         >  6:365091/1‑151 (MQ=255)
               cTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTgcg                                                                                                                                        >  3:481049/1‑151 (MQ=255)
                 ggATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGcgcg                                                                                                                                      <  5:1255429/151‑1 (MQ=255)
                   aTCAGGAAGAAAAGTAGATACCGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCAAGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGcgcg                                                                                                                                      >  4:833592/1‑149 (MQ=255)
                   aTCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGac                                                                                                                                    >  1:1335623/1‑150 (MQ=255)
                   aTCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAg                                                                                                                                    <  1:1014572/151‑1 (MQ=255)
                     cAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCa                                                                                                                                  <  7:137918/151‑1 (MQ=255)
                     cAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGc                                                                                                                                   <  6:1246910/150‑1 (MQ=255)
                      aGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCAc                                                                                                                                 >  5:2132607/1‑151 (MQ=255)
                      aGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCAc                                                                                                                                 >  8:1494034/1‑151 (MQ=255)
                       ggAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCAcc                                                                                                                                >  5:1458815/1‑150 (MQ=255)
                       ggAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACg                                                                                                                                >  2:1081923/1‑151 (MQ=255)
                         aagaaAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGAt                                                                                                                              >  5:1646680/1‑151 (MQ=255)
                         aagaaAAGTAGATACAGCAGCATGGCGGGCAGCATCGACAGTACGCGTGCGTGAGGTGGGCTAACCACGGTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGAt                                                                                                                              <  6:764226/151‑1 (MQ=255)
                           gaaAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCg                                                                                                                            >  5:644098/1‑151 (MQ=255)
                           gaaAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCg                                                                                                                            <  1:1595316/151‑1 (MQ=255)
                           gaaAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCg                                                                                                                            >  7:860910/1‑151 (MQ=255)
                            aaaaGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCgg                                                                                                                           >  5:269065/1‑151 (MQ=255)
                             aaaGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGgt                                                                                                                          >  6:993843/1‑151 (MQ=255)
                               aGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGgtgt                                                                                                                        >  6:665188/1‑151 (MQ=255)
                                gTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTc                                                                                                                       <  1:308257/151‑1 (MQ=255)
                                      aCAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGTGTTAACCACGCTCAGCGGTACGACCATAAGAGACATCATAAACACGGTGAATACAAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTAAGTGtt                                                                                                                 >  4:1058493/1‑151 (MQ=255)
                                      aCAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGtt                                                                                                                 >  1:1512105/1‑151 (MQ=255)
                                       cagcagCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTc                                                                                                                >  2:1309479/1‑151 (MQ=255)
                                        agcagcATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTcc                                                                                                               >  4:1644180/1‑151 (MQ=255)
                                          cagcaTGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCcac                                                                                                             <  4:1175624/151‑1 (MQ=255)
                                              aTGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAAtg                                                                                                         <  6:1789823/151‑1 (MQ=255)
                                                 gctggcAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTgc                                                                                                      <  5:2037598/151‑1 (MQ=255)
                                                 gctggcAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTgc                                                                                                      <  6:8907/151‑1 (MQ=255)
                                                 gctggcAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTgc                                                                                                      <  7:1120114/151‑1 (MQ=255)
                                                  ctggcAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTgcg                                                                                                     >  2:1179878/1‑151 (MQ=255)
                                                   tggcAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTgcgc                                                                                                    >  8:1030643/1‑151 (MQ=255)
                                                   tggcAGCATCGACAGTACGCGTCCCTGACGTGCGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGTTGAATACCAACCTGATCAGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTgcgc                                                                                                    >  8:1357232/1‑151 (MQ=255)
                                                    ggcAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTgcg                                                                                                     <  8:2181241/149‑1 (MQ=255)
                                                          aTCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCa                                                                                             <  3:1136477/151‑1 (MQ=255)
                                                          aTCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCa                                                                                             <  2:2012797/151‑1 (MQ=255)
                                                          aTCGACAGTACGCGTCCCTGACGTGGGGTAACCACGCTCAGCGGTACGACCATACGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGGCAGTGTTCCACAATGTGCGCATGTCCa                                                                                             <  4:1230089/151‑1 (MQ=255)
                                                            cGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATc                                                                                           >  5:944200/1‑151 (MQ=255)
                                                             gACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCt                                                                                          >  5:451701/1‑151 (MQ=255)
                                                                 gTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTc                                                                                      <  4:132410/151‑1 (MQ=255)
                                                                   aCGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCg                                                                                     <  1:191971/150‑1 (MQ=255)
                                                                    cgcgTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGt                                                                                   >  7:1615439/1‑151 (MQ=255)
                                                                       ggcccTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGGCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATc                                                                                <  4:1926085/149‑1 (MQ=255)
                                                                          ccTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGtt                                                                             >  2:1392000/1‑151 (MQ=255)
                                                                             gACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTc                                                                            <  4:1736983/149‑1 (MQ=255)
                                                                             gACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCgg                                                                          >  8:743201/1‑151 (MQ=255)
                                                                               cGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGt                                                                        <  7:1912018/151‑1 (MQ=255)
                                                                                gTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTc                                                                       <  4:1465132/151‑1 (MQ=255)
                                                                                 tGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCg                                                                      >  3:1485333/1‑151 (MQ=255)
                                                                                  gggTTAACCTCTCTCAGCGTGACGACCATAAGTGACATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGGCGa                                                                     <  6:1534081/151‑1 (MQ=255)
                                                                                    gTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAgc                                                                   >  7:251296/1‑151 (MQ=255)
                                                                                     ttAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAgcg                                                                  >  1:1856146/1‑151 (MQ=255)
                                                                                         ccACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCAc                                                              >  8:1948182/1‑151 (MQ=255)
                                                                                           aCGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCg                                                            >  2:280376/1‑151 (MQ=255)
                                                                                            cGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTCGTCGGTATCGTTCGGGTCGAGCGCCACCGc                                                           >  8:2067867/1‑151 (MQ=255)
                                                                                             gCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGcc                                                          >  8:875482/1‑151 (MQ=255)
                                                                                                cAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTgg                                                       >  5:374544/1‑151 (MQ=255)
                                                                                                      tACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGAcc                                                  >  1:775818/1‑150 (MQ=255)
                                                                                                       aCGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGCTCGAGCGCCACCGCCTGGTGACCa                                                 >  5:1141864/1‑150 (MQ=255)
                                                                                                         gACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATg                                              >  2:1834776/1‑151 (MQ=255)
                                                                                                          aCCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATATGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGa                                             <  3:2194420/151‑1 (MQ=255)
                                                                                                           ccATAAGTGCCATCATAAACACGGTGAATACCAAGGTGATACGCCAGTTCAGTTCTGCGCGCGCACTATCGGAGTCAGTGTTCCACAATGTGCGCATGTCACGGTGGTAGGTATGGTTGGGGTCGAGCGCCACCGCCTGGTGACCAATgct                                            >  6:2222293/1‑149 (MQ=255)
                                                                                                           ccATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGAt                                            >  8:441105/1‑151 (MQ=255)
                                                                                                           ccATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGAt                                            <  1:47603/151‑1 (MQ=255)
                                                                                                           ccATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGAt                                            >  2:2121204/1‑151 (MQ=255)
                                                                                                             aTAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCg                                          >  4:751438/1‑151 (MQ=255)
                                                                                                              tAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTTGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGc                                         >  1:1090815/1‑151 (MQ=255)
                                                                                                               aaGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGcc                                        <  3:2011078/151‑1 (MQ=255)
                                                                                                                aGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCt                                       <  5:1009327/151‑1 (MQ=255)
                                                                                                                aGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCt                                       >  3:1607448/1‑151 (MQ=255)
                                                                                                                 gTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTg                                      >  2:79551/1‑151 (MQ=255)
                                                                                                                  tGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTg                                      >  7:643075/1‑150 (MQ=255)
                                                                                                                     catcatAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATa                                   >  7:419634/1‑150 (MQ=255)
                                                                                                                     catcatAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGGCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATaa                                  <  6:54371/151‑1 (MQ=255)
                                                                                                                      atcatAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAAt                                 >  4:469612/1‑151 (MQ=255)
                                                                                                                         atAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCt                              >  3:1059941/1‑151 (MQ=255)
                                                                                                                           aaaCACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTgg                            >  3:631943/1‑151 (MQ=255)
                                                                                                                             acacGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGa                           >  2:583066/1‑150 (MQ=255)
                                                                                                                             acacGGTGAATACCAACGTGATACGCCAGTTCAGGTCTGCGCGAGTACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGaa                          <  6:1983616/151‑1 (MQ=255)
                                                                                                                               acGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGt                        >  2:963772/1‑151 (MQ=255)
                                                                                                                                  gTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCg                     <  7:555271/151‑1 (MQ=255)
                                                                                                                                  gTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCg                     >  2:400441/1‑151 (MQ=255)
                                                                                                                                     aaTACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTa                   <  8:565926/150‑1 (MQ=255)
                                                                                                                                      aTACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAAt                 <  8:1182057/151‑1 (MQ=255)
                                                                                                                                      aTACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAAt                 <  7:1242650/151‑1 (MQ=255)
                                                                                                                                        aCCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATg                <  6:1083223/150‑1 (MQ=255)
                                                                                                                                            aCGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCGGaa           <  7:2140924/151‑1 (MQ=255)
                                                                                                                                             cGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCGGaaa          <  3:1816671/151‑1 (MQ=255)
                                                                                                                                              gTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCGGAAAt         <  7:1564100/151‑1 (MQ=255)
                                                                                                                                               tGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCGGAAATc        >  7:2025942/1‑151 (MQ=255)
                                                                                                                                                gATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCGGAAATc        <  7:900670/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CGATTTCAGGGAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCGGAAATCACGTAA  >  DH1/4026634‑4026933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑