Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,235,9820AG59.0% 358.8 / inf 553S4P (TCA→CCA) ghxPpermease
Reads supporting (aligned to +/- strand):  ref base A (117/110);  new base G (168/158);  total (285/268)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 573 total aligned reads displayed.

TGCTTTACCCAACATACCTGGAACGACGATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCAAAAAAGCAAACGTTTAGCTCCGCGCATACA  >  DH1/4235833‑4236132
                                                                                                                                                     |                                                                                                                                                      
      aCCCAACATACCTGGAACGACGATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGa                                                                                                                                                  <  3:887126/150‑1 (MQ=255)
      aCCCAACATACCTGGAACGACGATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGa                                                                                                                                                  <  7:549698/150‑1 (MQ=255)
      aCCCAACATACCTGGAACGACGATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGAc                                                                                                                                                 <  4:1150788/151‑1 (MQ=255)
       cccAACATACCTGGAACGACGATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGAc                                                                                                                                                 >  2:1139608/1‑150 (MQ=255)
        ccAACATACCTGGAGCGACGATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACAt                                                                                                                                               >  8:940558/1‑151 (MQ=255)
        ccAACATACCTGGAACGACGATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACAt                                                                                                                                               <  3:588991/151‑1 (MQ=255)
        ccAACATACCTGGAACGACGATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACAt                                                                                                                                               <  4:1983444/151‑1 (MQ=255)
         cAACATACCTGGAACGACGATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATa                                                                                                                                              <  1:1907323/151‑1 (MQ=255)
           aCATACCTGGAACGACGATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAg                                                                                                                                            <  8:718695/151‑1 (MQ=255)
            cATACCTGGAACGACGATGACCCAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGAGTCGAGTGAACCGCCGGTACTCGCTGATTGCGTAGACATa                                                                                                                                              >  6:545940/1‑148 (MQ=255)
             aTACCTGGAACGACGATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGaa                                                                                                                                          <  3:1979034/151‑1 (MQ=255)
             aTACCTGGAACGACGATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGa                                                                                                                                           >  4:2132369/1‑150 (MQ=255)
                ccTGGAACGACGATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAAc                                                                                                                                       >  3:1777669/1‑151 (MQ=255)
                  tGGAACGACGATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACAt                                                                                                                                     >  6:421135/1‑151 (MQ=255)
                   ggAACGACGATGACCGAGTAGACAATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACa                                                                                                                                      <  6:403438/149‑1 (MQ=255)
                     aaCGACGATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATccc                                                                                                                                  >  2:200933/1‑151 (MQ=255)
                      acgacgATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATcccc                                                                                                                                 >  6:1887540/1‑151 (MQ=255)
                       cgacgaTGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCt                                                                                                                                <  7:1755708/151‑1 (MQ=255)
                         acgaTGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTg                                                                                                                               >  5:432251/1‑150 (MQ=255)
                           gaTGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTgaga                                                                                                                            <  5:162996/151‑1 (MQ=255)
                            aTGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGagag                                                                                                                           <  6:755641/151‑1 (MQ=255)
                            aTGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGagag                                                                                                                           <  4:1212586/151‑1 (MQ=255)
                             tGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGt                                                                                                                          >  7:944056/1‑151 (MQ=255)
                                  gAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAAt                                                                                                                     >  7:1629847/1‑151 (MQ=255)
                                  gAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAAt                                                                                                                     <  4:312134/151‑1 (MQ=255)
                                   aGTAGACAATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCTCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAAtt                                                                                                                    >  4:905301/1‑151 (MQ=255)
                                    gTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAAttt                                                                                                                   <  8:383116/151‑1 (MQ=255)
                                       gACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCg                                                                                                                <  1:535259/151‑1 (MQ=255)
                                        aCCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGt                                                                                                               <  7:1927648/151‑1 (MQ=255)
                                         ccATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTc                                                                                                              >  7:1130599/1‑151 (MQ=255)
                                           aTCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCaa                                                                                                            <  6:1155035/151‑1 (MQ=255)
                                           aTCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCa                                                                                                             >  4:1497654/1‑150 (MQ=255)
                                              gCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGAt                                                                                                         <  7:1249302/151‑1 (MQ=255)
                                                cAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGc                                                                                                       >  6:259531/1‑151 (MQ=255)
                                                 aGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCg                                                                                                      <  6:1766794/151‑1 (MQ=255)
                                                    aaCGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTgg                                                                                                   <  3:933193/151‑1 (MQ=255)
                                                     aCGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGc                                                                                                  >  4:1004581/1‑151 (MQ=255)
                                                       gttgttAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCgg                                                                                                >  1:1139611/1‑151 (MQ=255)
                                                        ttgttAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCgg                                                                                                >  6:1360190/1‑150 (MQ=255)
                                                         tgttAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGa                                                                                               <  1:1039977/150‑1 (MQ=255)
                                                          gttAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGAc                                                                                              <  8:1177320/150‑1 (MQ=255)
                                                          gttAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACa                                                                                             <  6:867149/151‑1 (MQ=255)
                                                            aaaCCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACCTCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAAt                                                                                           <  8:1639068/150‑1 (MQ=255)
                                                             aaCCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATc                                                                                          >  8:28137/1‑151 (MQ=255)
                                                             aaCCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATc                                                                                          >  7:2117/1‑151 (MQ=255)
                                                             aaCCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAAt                                                                                           <  4:1716938/150‑1 (MQ=255)
                                                              aCCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATcc                                                                                         >  2:1479041/1‑151 (MQ=255)
                                                                 cGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCt                                                                                      >  2:262426/1‑151 (MQ=255)
                                                                 cGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCt                                                                                      >  2:1615420/1‑151 (MQ=255)
                                                                 cGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCt                                                                                      <  1:390551/151‑1 (MQ=255)
                                                                 cGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCt                                                                                      <  2:732661/151‑1 (MQ=255)
                                                                   gCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGc                                                                                    >  1:1486133/1‑151 (MQ=255)
                                                                      aCCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAg                                                                                 <  3:258331/151‑1 (MQ=255)
                                                                        cACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCg                                                                               <  5:1823480/151‑1 (MQ=255)
                                                                            tCCTGACAGACAGTGCTTCCACGTTGTGAAATTTTAAACAAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCTGACAATCCCCTGCCAGCGAAAc                                                                           >  5:931402/1‑151 (MQ=255)
                                                                                 aCGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTAt                                                                      <  1:311269/151‑1 (MQ=255)
                                                                                    gACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCtt                                                                   >  7:1411504/1‑151 (MQ=255)
                                                                                    gACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCtt                                                                   >  1:2118443/1‑151 (MQ=255)
                                                                                    gACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCtt                                                                   <  8:199482/151‑1 (MQ=255)
                                                                                     aCAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTc                                                                  <  3:1273310/151‑1 (MQ=255)
                                                                                        gTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTg                                                               >  2:963870/1‑151 (MQ=255)
                                                                                         tGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGc                                                              >  3:463542/1‑151 (MQ=255)
                                                                                          tcTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCCGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGACAAGAAGCGTGGCGGACAATACCCTGCCAGCGAAACGTTATCTTACTGCt                                                             >  4:1375402/2‑151 (MQ=255)
                                                                                           cTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTc                                                            >  8:1420449/1‑151 (MQ=255)
                                                                                              ccACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAg                                                         <  5:551250/151‑1 (MQ=255)
                                                                                              ccACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTcc                                                           >  5:1463540/1‑149 (MQ=255)
                                                                                               cACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAgg                                                        >  5:23384/1‑151 (MQ=255)
                                                                                                 cGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGtt                                                      <  1:693051/151‑1 (MQ=255)
                                                                                                    tgtgAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGc                                                   >  7:72347/1‑151 (MQ=255)
                                                                                                    tgtgAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTg                                                    <  7:1567756/150‑1 (MQ=255)
                                                                                                     gtgAAATTTTAACCCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTAGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGACACGGGATCTTCCTGCTCCAGGTTTGCg                                                  >  6:1264630/1‑151 (MQ=255)
                                                                                                     gtgAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGCCAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCg                                                  >  5:32603/1‑151 (MQ=255)
                                                                                                      tgAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCg                                                  >  2:1332781/1‑150 (MQ=255)
                                                                                                       gAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGAc                                                >  7:1017642/1‑151 (MQ=255)
                                                                                                        aaaTTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGAc                                                >  3:1243725/1‑150 (MQ=255)
                                                                                                          aTTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAAt                                             >  4:510692/1‑151 (MQ=255)
                                                                                                           ttttAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAAtt                                            >  8:1087374/1‑151 (MQ=255)
                                                                                                              tAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTtg                                          <  2:1243775/150‑1 (MQ=255)
                                                                                                               aaaCCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTtgct                                        >  4:2204746/1‑151 (MQ=255)
                                                                                                                  ccAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCATCAATTTGCTGCg                                     >  3:14457/1‑151 (MQ=255)
                                                                                                                     ggCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCg                                  <  6:2097718/151‑1 (MQ=255)
                                                                                                                      gCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGc                                 >  1:1311001/1‑151 (MQ=255)
                                                                                                                        gTCGAGTGAACCGCCGGTACGCGCTGATGGTGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCa                                >  5:1067542/1‑150 (MQ=255)
                                                                                                                         tCGAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCaa                               >  5:1216017/1‑150 (MQ=255)
                                                                                                                          cGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCaaaa                             <  8:585286/151‑1 (MQ=255)
                                                                                                                           gAGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCaaaa                             >  2:487927/1‑150 (MQ=255)
                                                                                                                            aGTGAACCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCaaaaa                            >  8:1872383/1‑150 (MQ=255)
                                                                                                                             gTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCAAAAAAg                          <  5:668682/151‑1 (MQ=255)
                                                                                                                                 aCCGCCGGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCAAAAAAGCaaa                      <  1:833366/151‑1 (MQ=255)
                                                                                                                                 aCCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGAACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCAAAAAAGCaaa                      <  5:206193/151‑1 (MQ=255)
                                                                                                                                   cgccgGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCAAAAAAGCAAACg                    >  5:826348/1‑151 (MQ=255)
                                                                                                                                   cgccgGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCAAAAAAGCAAACg                    >  2:2092/1‑151 (MQ=255)
                                                                                                                                      cgGTACGCGCTGATGGCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCAAAAAAGCAAACGttt                 <  3:378527/151‑1 (MQ=255)
                                                                                                                                        gTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCAAAAAAGCAAACGttt                 >  3:1177577/1‑149 (MQ=255)
                                                                                                                                          aCGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGNAACGTTNTCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCAAAAAAGCAAACGTTTAGCt             >  2:1782645/1‑151 (MQ=255)
                                                                                                                                          aCGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCAAAAAAGCAAACGTTTAGc              <  4:1794167/150‑1 (MQ=255)
                                                                                                                                                 gatggCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCAAAAAAGCAAACGTTTAGCTCCgcgc       <  2:184999/150‑1 (MQ=255)
                                                                                                                                                  atgaCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCAAAAAAGCAAACGTTTAGCTCCGCGCAt     >  6:1458923/1‑151 (MQ=255)
                                                                                                                                                  atgaCGTAGACATAAGAAAACAACCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATATTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCAAAAAAGCAAACGTTTAGATCCGCGCAt     >  8:541045/1‑151 (MQ=255)
                                                                                                                                                     aCGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCAAAAAAGCAAACGTTTAGCTCCGCGCATAc   >  7:293202/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TGCTTTACCCAACATACCTGGAACGACGATGACCGAGTAGACCATCGCCAGAAACGTTGTTAACCCGGCAACCACTTCCTGACGGACAGTGCTTCCACGTTGTGAAATTTTAAACCAGGCGTCGAGTGAACCGCCGGTACGCGCTGATGACGTAGACATAAGAAAACATCCCCTGAGAGTTTAATTTTCGTCAAGATGCGTGGCGGACAATCCCCTGCCAGCGAAACGTTATCTTCCTGCTCCAGGTTTGCGACAATTTGCTGCGTCGCAAAAAAGCAAACGTTTAGCTCCGCGCATACA  >  DH1/4235833‑4236132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑