BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	4,320,650	0	A	G	62.7%	543.9 / inf	595	R129G (AGA→GGA)	thiE	thiamine phosphate synthase
Reads supporting (aligned to +/- strand): ref base A (121/100); new base G (187/184); total (310/285)
Fisher's exact test for biased strand distribution p-value = 3.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.21e-02

Only 100 of 609 total aligned reads displayed.

CAGGCGTATGGCGTCCATTTGGGGCAGGAAGATTTGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGCGGTGATAGCAACGG  >  DH1/4320500‑4320800

 aGGCGTATGGCGTCCATTTGGGGCAGGAAGATTTGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTgag                                                                                                                                                       <  1:1144814/151‑1 (MQ=255)

       aTGGCGTCCATTTGGGGCAGGAAGATTTGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACAtgt                                                                                                                                                 <  5:237429/151‑1 (MQ=255)

         ggCGTCCATTTGGGGCAGGAAGATTTGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACGtgtg                                                                                                                                                <  8:1502665/150‑1 (MQ=255)

         ggCGTCCATTTGGGGCAGGAAGATTTGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACAtgtg                                                                                                                                                <  2:837209/150‑1 (MQ=255)

             tCCATTTGGGGCAGGAAGATTTGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATgtgtaacc                                                                                                                                           >  2:130999/1‑147 (MQ=255)

              ccATTTGGGGCAGGAAGATTTGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCg                                                                                                                                          >  7:1999076/1‑151 (MQ=255)

                 tttGGGGCAGGAAGATTTGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCCACg                                                                                                                                       >  1:100273/1‑151 (MQ=255)

                  ttGGGGCAGGAAGATTTGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGc                                                                                                                                      >  4:1241213/1‑151 (MQ=255)

                        cAGGAAGATTTGCAAGCCACCGATCTCATTGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGcaaacca                                                                                                                                <  2:237216/151‑1 (MQ=255)

                        cAGGAAGATTTGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGcaaacc                                                                                                                                 >  4:287161/1‑150 (MQ=255)

                          ggAAGATTTGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGcaaaccaaa                                                                                                                              <  5:1477861/151‑1 (MQ=255)

                          ggAAGATTTGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGcaaaccaa                                                                                                                               >  6:352823/1‑150 (MQ=255)

                           gAAGATTTGCAAGCCACCGATCTCAATGCCAACCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGcaaaccaaac                                                                                                                             <  8:2090107/151‑1 (MQ=255)

                            aaGATTTGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGcaaaccaaac                                                                                                                             >  7:228492/1‑150 (MQ=255)

                            aaGATTTGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGcaaaccaaac                                                                                                                             >  3:462401/1‑150 (MQ=255)

                                 ttGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATg                                                                                                                        <  6:682269/150‑1 (MQ=255)

                                 ttGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATg                                                                                                                        <  4:1349152/150‑1 (MQ=255)

                                   gCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGcc                                                                                                                      <  1:488356/150‑1 (MQ=255)

                                    cAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCtt                                                                                                                    <  5:969072/151‑1 (MQ=255)

                                      aGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCt                                                                                                                  <  5:1088097/151‑1 (MQ=255)

                                      aGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCt                                                                                                                  <  2:2082922/151‑1 (MQ=255)

                                         cACCGATCTCACTGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCa                                                                                                               <  2:1965802/151‑1 (MQ=255)

                                              aTCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGc                                                                                                           >  8:469215/1‑150 (MQ=255)

                                               tctcAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGc                                                                                                           >  4:1865606/1‑149 (MQ=255)

                                                  cAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAgg                                                                                                        <  1:766181/149‑1 (MQ=255)

                                                  cAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCa                                                                                                          <  8:115192/147‑1 (MQ=255)

                                                   aaTGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGc                                                                                                     <  4:700267/151‑1 (MQ=255)

                                                   aaTGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGc                                                                                                     >  4:217938/1‑151 (MQ=255)

                                                   aaTGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGc                                                                                                     <  3:936276/151‑1 (MQ=255)

                                                   aaTGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGGTTCGACACATGAAGATATGGAGATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGc                                                                                                     >  1:62804/1‑151 (MQ=255)

                                                    aTGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCt                                                                                                    <  1:1526038/151‑1 (MQ=255)

                                                     tGCCATCCGCGCGGCAGGCCTGCGGCTAGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTg                                                                                                   >  1:2169262/1‑151 (MQ=255)

                                                      gCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTgg                                                                                                  <  1:2002750/151‑1 (MQ=255)

                                                         aTCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAAc                                                                                               >  2:696670/1‑151 (MQ=255)

                                                           ccGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACa                                                                                              <  4:108137/150‑1 (MQ=255)

                                                           ccGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACa                                                                                              <  5:1280927/150‑1 (MQ=255)

                                                           ccGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACa                                                                                              <  6:1864442/150‑1 (MQ=255)

                                                            cgcgcgGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGc                                                                                            >  3:789035/1‑151 (MQ=255)

                                                             gcgcgGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCt                                                                                           <  1:397120/151‑1 (MQ=255)

                                                             gcgcgGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCt                                                                                           >  7:1065886/1‑151 (MQ=255)

                                                              ggcgGCAGTCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCATACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTg                                                                                          <  6:2150735/150‑1 (MQ=255)

                                                                cgGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGc                                                                                        <  7:1426458/151‑1 (MQ=255)

                                                                      gCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGc                                                                                   >  2:1429990/1‑150 (MQ=255)

                                                                      gCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGc                                                                                   >  5:216735/1‑150 (MQ=255)

                                                                       ccTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCAt                                                                                 <  3:1559209/151‑1 (MQ=255)

                                                                        cTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATg                                                                                >  6:1938723/1‑151 (MQ=255)

                                                                        cTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATg                                                                                >  3:1872143/1‑151 (MQ=255)

                                                                        cTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATg                                                                                >  3:203579/1‑151 (MQ=255)

                                                                        cTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATg                                                                                >  4:904406/1‑151 (MQ=255)

                                                                          gCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGtt                                                                              <  2:423800/151‑1 (MQ=255)

                                                                           cGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTg                                                                             <  6:2190959/151‑1 (MQ=255)

                                                                           cGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTg                                                                             <  7:187180/151‑1 (MQ=255)

                                                                             gCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTg                                                                             >  2:55048/1‑149 (MQ=255)

                                                                             gCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTg                                                                             >  7:528832/1‑149 (MQ=255)

                                                                                   cGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTg                                                                     >  5:1896437/1‑151 (MQ=255)

                                                                                      ttCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGc                                                                   <  6:1464291/150‑1 (MQ=255)

                                                                                          acacATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGAtt                                                              >  8:318245/1‑151 (MQ=255)

                                                                                            acaTGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTAt                                                            <  4:1437187/151‑1 (MQ=255)

                                                                                            acaTGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTAt                                                            >  7:1732226/1‑151 (MQ=255)

                                                                                               tGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATccc                                                         >  3:2172562/1‑151 (MQ=255)

                                                                                                   gATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTAGAGCGACTGGCGGATTATCCCACCg                                                     >  8:1745633/1‑151 (MQ=255)

                                                                                                        ggAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGc                                                 <  4:213878/150‑1 (MQ=255)

                                                                                                          aaaTCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGAt                                              <  4:462547/151‑1 (MQ=255)

                                                                                                          aaaTCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGAt                                              >  2:2028849/1‑151 (MQ=255)

                                                                                                          aaaTCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGAt                                              >  3:370697/1‑151 (MQ=255)

                                                                                                           aaTCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGAtt                                             <  3:1123522/151‑1 (MQ=255)

                                                                                                              ggACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCTGATTATCCCACCGTGGCTATTgg                                           <  4:977601/149‑1 (MQ=255)

                                                                                                                 cGTCGCGCTGGCAGCACGCCCCTCTTATATCGAGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACAGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGt                                       >  5:1625051/1‑151 (MQ=255)

                                                                                                                  gTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTa                                      <  4:1951879/151‑1 (MQ=255)

                                                                                                                   tCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTAt                                     >  8:137728/1‑151 (MQ=255)

                                                                                                                   gcgcgCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTAt                                     <  7:307545/150‑1 (MQ=255)

                                                                                                                       gcTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCGAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGt                                 <  8:58361/151‑1 (MQ=255)

                                                                                                                        cTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTc                                >  2:1186206/1‑151 (MQ=255)

                                                                                                                          ggCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGACCGACTGGCGGATTATCCAACCGTGGCGATTGGCGGTATCAGTCTg                              >  3:1324397/1‑151 (MQ=255)

                                                                                                                               caCGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCAcg                         <  7:898319/151‑1 (MQ=255)

                                                                                                                               caCGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCAcg                         >  8:1611921/1‑151 (MQ=255)

                                                                                                                               caCGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCAcg                         <  8:780642/151‑1 (MQ=255)

                                                                                                                                aCGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCAcgc                        >  5:615431/1‑151 (MQ=255)

                                                                                                                                 cGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGTGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCAcgcg                       <  7:1223325/151‑1 (MQ=255)

                                                                                                                                  gCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCAcgcgc                      >  7:1554204/1‑151 (MQ=255)

                                                                                                                                  gCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCAcgcgc                      <  7:387758/151‑1 (MQ=255)

                                                                                                                                     ccTCTTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGcc                   >  6:1261965/1‑151 (MQ=255)

                                                                                                                                      ctctTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCCCGCGCGCCt                  >  2:1729923/1‑151 (MQ=255)

                                                                                                                                      ctctTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGccg                  >  8:672643/1‑150 (MQ=255)

                                                                                                                                        ctTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGc                >  2:1023615/1‑151 (MQ=255)

                                                                                                                                        ctTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGc                >  6:1642073/1‑151 (MQ=255)

                                                                                                                                        ctTATATCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGc                >  4:366380/1‑151 (MQ=255)

                                                                                                                                        ctTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGc                >  6:1742628/1‑151 (MQ=255)

                                                                                                                                         ttATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGCg               >  5:739175/1‑151 (MQ=255)

                                                                                                                                          tctatCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGc                <  4:1660040/147‑1 (MQ=255)

                                                                                                                                           atatCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGCgg              <  1:1777713/150‑1 (MQ=255)

                                                                                                                                           atatCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGCGGt             >  7:679848/1‑151 (MQ=255)

                                                                                                                                           atatCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGCGGt             >  1:1339305/1‑151 (MQ=255)

                                                                                                                                           atatCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGCGGt             >  3:101867/1‑151 (MQ=255)

                                                                                                                                             atCGCGCTGGTACATGTGTTCGCGAGGCAAACCAAACAGATGCCTTCTGGACCGGAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGAGTGGCGGATTATCCCACCGTGGAGATTGGCGGTATCAGTCTGGCACGCGCGCCTGCGGTGa           <  3:1723684/151‑1 (MQ=255)

                                                                                                                                             atCGCGCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGCGGTg            <  2:1594213/150‑1 (MQ=255)

                                                                                                                                               cgcgCTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGCGGTGATa         <  5:517605/151‑1 (MQ=255)

                                                                                                                                                  gcTGGGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGTCACTGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGCGGTGATAGCa      <  6:858227/151‑1 (MQ=255)

                                                                                                                                                  gcTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGCGGTGATAGc       >  3:88389/1‑150 (MQ=255)

                                                                                                                                                     gagaCATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGCGGTGATAGCAACg   <  5:353474/151‑1 (MQ=255)

CAGGCGTATGGCGTCCATTTGGGGCAGGAAGATTTGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGTTTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGAGACATGTGTTCCCGACGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCCACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGCGGTGATAGCAACGG  >  DH1/4320500‑4320800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`