Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,338,6430AG60.6% 544.7 / inf 701noncoding (74/76 nt)ECDH1_RS21430tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (150/126);  new base G (225/200);  total (375/326)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.40e-01

Only 100 of 707 total aligned reads displayed.

GCGGTTTTGTACGTTCAAACTTTTCTTTAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGCTGAGGTAATAGCCATTATACGATG  >  DH1/4338493‑4338792
                                                                                                                                                      |                                                                                                                                                     
      ttGTACGTTCAAACTTTTCTTTAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTg                                                                                                                                                  <  7:539338/150‑1 (MQ=255)
       tGTACGTTCAAACTTTTCTTTAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGa                                                                                                                                                 <  3:1937952/150‑1 (MQ=255)
           cGTTCAAACTTTTCTTTAGACATCTATTGTCCCTCTAAGAGACGGATAAATCGTTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAgg                                                                                                                                             <  6:393435/150‑1 (MQ=255)
              tCAAACTTTTCTTTAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGc                                                                                                                                            >  5:562843/1‑148 (MQ=255)
              tCAAACTTTTCTTTAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAg                                                                                                                                          <  4:1263507/150‑1 (MQ=255)
                ataCTTTTGTTTATACATCGTTTGTCCCTGTAAGACACGGATAAATCTTTGATATCAGCACATCAACCAGGCAAGATGGGGGTCTTGGTGAATGCAATAAACAGAAGGAAAAAACAGGTAGTAGAAAAGAAGTGGTGCTGATAGGCAGAtt                                                                                                                                       <  4:785410/149‑1 (MQ=255)
                aaaCTTTTCTTTAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGAtt                                                                                                                                       <  6:245664/151‑1 (MQ=255)
                 aaCTTTTCTTTAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTc                                                                                                                                      <  4:654600/151‑1 (MQ=255)
                  aCTTTTCTTTAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCg                                                                                                                                     >  2:883463/1‑151 (MQ=255)
                  aCTTTTCTTTAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCg                                                                                                                                     <  5:102411/151‑1 (MQ=255)
                  aCTTTTCTTTAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCg                                                                                                                                     >  7:1404386/1‑151 (MQ=255)
                  aCTTTTCTTTAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCg                                                                                                                                     <  6:1196734/151‑1 (MQ=255)
                   catttCTTTAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCAGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGa                                                                                                                                    <  2:198794/149‑1 (MQ=255)
                    ttttCTTTAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGa                                                                                                                                    >  1:940742/1‑150 (MQ=255)
                     tttCTTTAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAAc                                                                                                                                  >  8:405129/1‑151 (MQ=255)
                         tttAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATCCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGcc                                                                                                                              >  7:772052/1‑151 (MQ=255)
                         tttAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGACCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGcc                                                                                                                              >  5:1792219/1‑151 (MQ=255)
                            aGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGa                                                                                                                            <  2:2202617/150‑1 (MQ=255)
                            aGACATCGATTGTCCCTCTAAGACACGGATAAAT‑GGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGAcc                                                                                                                          >  6:1116968/1‑151 (MQ=255)
                             gACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGAcc                                                                                                                          <  7:1650892/151‑1 (MQ=255)
                                aTCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTc                                                                                                                        >  8:1095642/1‑150 (MQ=255)
                                aTCGATTGTCCCTCTAAGACACGGATAAATCGGTGATAACACCACATCAAACAGGCAACATGCCCGACTTGTTGAATTCAATAAACAGAAGTAAAAAACAGGGAGGAGAAAAGAAGTGGTTCTGATAGGCAGATTCGAACTGCCGACCTCa                                                                                                                       <  6:1486425/151‑1 (MQ=255)
                                  cGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCAc                                                                                                                      <  3:863774/150‑1 (MQ=255)
                                   gATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCAccc                                                                                                                    >  6:2149775/1‑151 (MQ=255)
                                    aTTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCt                                                                                                                   >  7:825290/1‑151 (MQ=255)
                                      tGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCtt                                                                                                                  >  8:1144016/1‑150 (MQ=255)
                                      tGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCtt                                                                                                                  <  3:983496/150‑1 (MQ=255)
                                       gTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTAc                                                                                                                <  4:162972/151‑1 (MQ=255)
                                       gTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTAc                                                                                                                <  8:2149357/151‑1 (MQ=255)
                                       gTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTAc                                                                                                                <  5:670118/151‑1 (MQ=255)
                                          ccTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCaa                                                                                                             <  3:533100/151‑1 (MQ=255)
                                          ccTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCa                                                                                                              >  1:771001/1‑150 (MQ=255)
                                          ccTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCa                                                                                                              >  8:1281970/1‑150 (MQ=255)
                                            tctAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAgg                                                                                                           >  1:1108383/1‑151 (MQ=255)
                                               aaGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCTGACCTCACCCTTACCAAGGGt                                                                                                         <  2:1406035/150‑1 (MQ=255)
                                               aaGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTg                                                                                                        >  3:21164/1‑151 (MQ=255)
                                               aaGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAgg                                                                                                           <  2:755480/148‑1 (MQ=255)
                                                aGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTgc                                                                                                       <  5:31128/151‑1 (MQ=255)
                                                aGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTgc                                                                                                       <  7:1895046/151‑1 (MQ=255)
                                                aGACACCGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTgc                                                                                                       <  4:330375/151‑1 (MQ=255)
                                                  acacGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGTAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTgcgc                                                                                                     >  1:1462473/1‑151 (MQ=255)
                                                      ggATATATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTa                                                                                                 <  6:52822/151‑1 (MQ=255)
                                                          aaaTCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGCTGCGCTCTACCa                                                                                              >  1:1672036/1‑150 (MQ=255)
                                                           aaTCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGAGTGCGCTCTACCAAc                                                                                            >  1:487394/1‑151 (MQ=255)
                                                                gTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGc                                                                                       <  4:2121570/151‑1 (MQ=255)
                                                                  gATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCta                                                                                     <  5:862897/151‑1 (MQ=255)
                                                                  gATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCt                                                                                      <  7:2191387/150‑1 (MQ=255)
                                                                  gATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCt                                                                                      >  1:1805017/1‑150 (MQ=255)
                                                                   atatCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCta                                                                                     <  8:1385055/150‑1 (MQ=255)
                                                                     atCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCtata                                                                                   >  1:613109/1‑150 (MQ=255)
                                                                       caccacATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATc                                                                                 >  2:1650517/1‑150 (MQ=255)
                                                                             aTCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACAt                                                                          >  5:1349279/1‑151 (MQ=255)
                                                                               cAACCAGGCAACATGCCCGACTTGTTGAATGCAGTAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCt                                                                        >  3:1548097/1‑151 (MQ=255)
                                                                               cAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGGAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTAAATCAGCACATCt                                                                        <  4:1457292/151‑1 (MQ=255)
                                                                                aaCCAGGCAACATGCCCGTCTTGTTGAATGCAATAAACAGAATGAAAAAACAGGTAGGAGAAAAGAAGTGATGCTGATAGTCAGATTCGAACTGCCGACCTAACCCTTACCAAGGGTGCGCTCTACCCACTGAGCTATATCAGCACATCtt                                                                       <  8:659345/151‑1 (MQ=37)
                                                                                      gCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGATGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCg                                                                 <  2:619508/151‑1 (MQ=255)
                                                                                      gCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCGATATCAGCACATCTTGGAGCg                                                                 >  8:269290/1‑151 (MQ=255)
                                                                                       cAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCgg                                                                >  5:1119601/1‑151 (MQ=255)
                                                                                       cAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCgg                                                                >  5:2223533/1‑151 (MQ=255)
                                                                                        aaCATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCggg                                                               >  8:1622229/1‑151 (MQ=255)
                                                                                         aCATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGc                                                              >  7:914241/1‑151 (MQ=255)
                                                                                           aTGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGc                                                              >  8:1947078/1‑149 (MQ=255)
                                                                                            tGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGc                                                           >  5:1223242/1‑151 (MQ=255)
                                                                                                cGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGATATATCAGCACATCTTGGAGCGGGCAGCGGGa                                                       <  6:992874/151‑1 (MQ=255)
                                                                                                 gACTTGTTGAATGCAATAAACAGAAGGAAAAAAGAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAAGTGCCGACCTCACCCTTACCAAGGGTGCGCGCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGaa                                                      <  4:1814859/151‑1 (MQ=255)
                                                                                                 gACTTGTTGAATGCAATAAACAGAAGGAAAAAACACGGAGGAGAAAAGAAGTGATCCTGATAGGCTGATTCGAAATCCCGACCTCACCCTTACCAAGGCTGCGCTCTACCAACTGAGCTATATCACCACATCTTCGAGCGGGCacccgca                                                       >  6:1846032/1‑144 (MQ=255)
                                                                                                   cTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATc                                                    >  1:1008588/1‑151 (MQ=255)
                                                                                                     tgttgAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCg                                                   >  7:392409/1‑150 (MQ=255)
                                                                                                      gttgAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGa                                                  >  7:117102/1‑150 (MQ=255)
                                                                                                        tgAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAAc                                                <  8:1514972/150‑1 (MQ=255)
                                                                                                          aaTGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCg                                             <  5:1499121/151‑1 (MQ=255)
                                                                                                          aaTGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGCAGCGGGCAGCGGGAATCGAACCCg                                             >  1:2164966/1‑151 (MQ=255)
                                                                                                           aTGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGc                                            >  3:1196967/1‑151 (MQ=255)
                                                                                                             gCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGcat                                          >  1:291368/1‑151 (MQ=255)
                                                                                                              cAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGcatc                                         <  4:873172/151‑1 (MQ=255)
                                                                                                              cAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGcatc                                         <  5:1584132/151‑1 (MQ=255)
                                                                                                               aaTAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGcatc                                         >  3:1357144/1‑150 (MQ=255)
                                                                                                                 tAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCatcatc                                      >  8:859047/1‑151 (MQ=255)
                                                                                                                  aaaCAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCAtcatca                                     <  3:372777/151‑1 (MQ=255)
                                                                                                                   aaCAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGCACCCGCatcatc                                      >  5:767579/1‑149 (MQ=255)
                                                                                                                    aCAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGc                                   <  3:703309/151‑1 (MQ=255)
                                                                                                                          ggAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGa                              >  1:1937194/1‑150 (MQ=255)
                                                                                                                           gAAAAAACAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAg                            <  7:1271041/151‑1 (MQ=255)
                                                                                                                            aaaaaaCAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAgg                           >  2:395051/1‑151 (MQ=255)
                                                                                                                             aaaaaCAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGc                          <  1:1364501/151‑1 (MQ=255)
                                                                                                                              aaaaCAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGCACCCGCATCATCAGCTTGGAAGGCt                         >  6:631831/1‑151 (MQ=255)
                                                                                                                              aaaaCAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGCt                         >  7:1739216/1‑151 (MQ=255)
                                                                                                                                aaCAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGCTGa                       <  3:180951/151‑1 (MQ=255)
                                                                                                                                aaCAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGCTGa                       <  4:1904182/151‑1 (MQ=255)
                                                                                                                                 aCAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGCTGAg                      <  2:1839690/151‑1 (MQ=255)
                                                                                                                                  cAGGGAGGAGAAAAGAAGTGGTGCTGATTGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGCTGAgg                     <  5:1966939/151‑1 (MQ=255)
                                                                                                                                  cAGGGAGGAGAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGCTGAgg                     <  3:1079783/151‑1 (MQ=255)
                                                                                                                                    gggAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGCTGAGGt                    >  7:1533388/1‑150 (MQ=255)
                                                                                                                                       aggagAAAAGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGCTGAGGTAATa                <  2:1275697/151‑1 (MQ=255)
                                                                                                                                           gaAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGCTGAGGTAATAGcc             >  3:2034716/1‑150 (MQ=255)
                                                                                                                                             aaaGAAGTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGCTGAGGTAATAGCCAtt          >  2:50383/1‑151 (MQ=255)
                                                                                                                                              aagaagTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGCTGAGGTAATAGCCATta         <  2:1107484/151‑1 (MQ=255)
                                                                                                                                               agaagTGGTGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGCTGAGGTAATAGCCATtat        <  6:1687786/151‑1 (MQ=255)
                                                                                                                                                gaagTGGTGCTGATAGGCAGATTCGAACTGGCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGCTGAGGTAATAGCCATtata       <  3:2187439/151‑1 (MQ=255)
                                                                                                                                                     gATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGTGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGAGGGAATCGAACCCGCATCATCAGCTTGGAAGGCTGAGGTAATAGCCATTATACGATg  <  4:522142/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCGGTTTTGTACGTTCAAACTTTTCTTTAGACATCGATTGTCCCTCTAAGACACGGATAAATCGGTGATATCACCACATCAACCAGGCAACATGCCCGACTTGTTGAATGCAATAAACAGAAGGAAAAAACAGGGAGGAGAAAAGAAGTGATGCTGATAGGCAGATTCGAACTGCCGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCTATATCAGCACATCTTGGAGCGGGCAGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGCTGAGGTAATAGCCATTATACGATG  >  DH1/4338493‑4338792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑