Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1755,7820TC60.6% 419.1 / inf 589L14P (CTC→CCC) glcDFAD‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (117/113);  new base C (161/193);  total (281/308)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-07

Only 100 of 590 total aligned reads displayed.

GACCGGTAGCTAAAGAGATAGACGAAAACGAAAAGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCACGCGTCCATTACTGGTTGTTCTGCCTA  >  DH1/755632‑755928
                                                                                                                                                      |                                                                                                                                                  
   cGGTAGCTAAAGAGATAGACGAAAACGAAAAGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGa                                                                                                                                                 <  7:1897992/151‑1 (MQ=255)
   cGGTAGCTAAAGAGATAGACGAAAACGAAAAGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCg                                                                                                                                                  >  1:2140618/1‑150 (MQ=255)
        gCTAAAGAGATAGACGAAAACGAAAAGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCg                                                                                                                                            <  2:1425986/151‑1 (MQ=255)
        gCTAAAGAGATAGACGAAAACGAAAAGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCg                                                                                                                                            <  2:731846/151‑1 (MQ=255)
           aaaGAGATAGACGAAAACTAAAAGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACTACACAACAAATCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGAcc                                                                                                                                         <  4:1085524/151‑1 (MQ=255)
           aaaGAGATAGACGAAAACGAAAAGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGAcc                                                                                                                                         >  3:1315669/1‑151 (MQ=255)
            aaGAGATAGACGAAAACGAAAAGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACCACACAACAAAGCGAAGCCTACTCATGAGCATCTTCTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCg                                                                                                                                        >  4:1620380/1‑151 (MQ=255)
                   aGACGAAAACGAAAAGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATc                                                                                                                                  <  1:1681812/150‑1 (MQ=255)
                     acgaaaacgaaaAGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGt                                                                                                                               <  6:2167263/151‑1 (MQ=255)
                       gaaaacgaaaaGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTAc                                                                                                                             >  7:25441/1‑151 (MQ=255)
                        aaaacgaaaaGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACt                                                                                                                            >  1:1027599/1‑151 (MQ=255)
                         aaacgaaaaGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTg                                                                                                                           <  6:768378/151‑1 (MQ=255)
                         aaacgaaaaGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTg                                                                                                                           >  1:887450/1‑151 (MQ=255)
                         aaacgaaaaGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTg                                                                                                                           <  1:33851/151‑1 (MQ=255)
                           acgaaaaGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGAt                                                                                                                         <  5:2197359/151‑1 (MQ=255)
                            cgaaaaGCCCGCTTAATAACTGTTCACAGAACCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTCTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATg                                                                                                                        <  8:594442/151‑1 (MQ=255)
                              aaaaGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGc                                                                                                                      >  6:1620414/1‑151 (MQ=255)
                               aaaGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGc                                                                                                                      >  5:680344/1‑150 (MQ=255)
                               aaaGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGc                                                                                                                      >  1:1548521/1‑150 (MQ=255)
                                  gCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTg                                                                                                                  <  6:2001411/151‑1 (MQ=255)
                                  gCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTg                                                                                                                  <  2:2101297/151‑1 (MQ=255)
                                   cccGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGc                                                                                                                 >  1:1945407/1‑151 (MQ=255)
                                   cccGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGc                                                                                                                 <  5:1797451/151‑1 (MQ=255)
                                     cGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACAGCACATCGGTACTGATGGCACTGCGt                                                                                                               >  3:1847081/1‑151 (MQ=255)
                                      gCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTg                                                                                                              >  7:1774092/1‑151 (MQ=255)
                                        ttAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACCACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACAGCACATCGGTACTGATGGCACTGCGTg                                                                                                              >  3:1416467/1‑149 (MQ=255)
                                         taataaCTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACAGCGTGAGc                                                                                                           >  4:337104/1‑151 (MQ=255)
                                          aataaCTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGc                                                                                                           >  4:113061/1‑150 (MQ=255)
                                          aataaCTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGc                                                                                                           >  8:1995916/1‑150 (MQ=255)
                                          aataaCTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGc                                                                                                           <  1:1823039/150‑1 (MQ=255)
                                            taaCTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATg                                                                                                        >  8:825397/1‑151 (MQ=255)
                                               cTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTcc                                                                                                     >  6:328459/1‑151 (MQ=255)
                                                 gTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTccc                                                                                                    <  7:1281341/150‑1 (MQ=255)
                                                 gTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTccc                                                                                                    <  1:1376201/150‑1 (MQ=255)
                                                 gTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCt                                                                                                   >  2:1617221/1‑151 (MQ=255)
                                                  ttCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTg                                                                                                  >  5:1374041/1‑151 (MQ=255)
                                                    cacaGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTgg                                                                                                 >  3:2151042/1‑150 (MQ=255)
                                                    cacaGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGa                                                                                                <  5:816199/151‑1 (MQ=255)
                                                    cacaGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGa                                                                                                <  3:2099915/151‑1 (MQ=255)
                                                        gAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTg                                                                                            <  6:458991/151‑1 (MQ=255)
                                                        gAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTg                                                                                            <  3:1650343/151‑1 (MQ=255)
                                                         aaGCAGCGCTCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTga                                                                                           <  5:1100699/151‑1 (MQ=255)
                                                         aaGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTGCTGATGGCACTGCGTGAGCATGTCCCTGGACTTg                                                                                            >  8:239748/1‑150 (MQ=255)
                                                         aaGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTg                                                                                            >  1:2049344/1‑150 (MQ=255)
                                                          cgcagcGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTgag                                                                                          <  7:2159655/150‑1 (MQ=255)
                                                            cagcGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGAt                                                                                        <  1:1120145/151‑1 (MQ=255)
                                                            cagcGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGAt                                                                                        <  5:1295109/151‑1 (MQ=255)
                                                             agcGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGAACTGATCGCACTGCGTGAGCATGTCCCTGGACTTg                                                                                            >  4:541725/1‑146 (MQ=255)
                                                                gcgcAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGCTACTGATGGCACTGCCTGAGCATGTCCCTGGACTTGAGATCCTg                                                                                    >  8:1615130/1‑151 (MQ=255)
                                                                   cAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCAt                                                                                 <  3:1198747/151‑1 (MQ=255)
                                                                    aaaaaTCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCAt                                                                                 >  1:241626/1‑150 (MQ=255)
                                                                    aaaaaTCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCAt                                                                                 >  2:488419/1‑150 (MQ=255)
                                                                           aGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATg                                                                          >  7:856233/1‑150 (MQ=255)
                                                                            gCTGCCACTCAACAGAACTAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATgag                                                                        <  2:36483/151‑1 (MQ=255)
                                                                            gCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATgag                                                                        <  2:1295343/151‑1 (MQ=255)
                                                                            gCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATgag                                                                        <  2:1114961/151‑1 (MQ=255)
                                                                            gCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATgag                                                                        <  6:901620/151‑1 (MQ=255)
                                                                             cTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATgagg                                                                       >  6:619654/1‑151 (MQ=255)
                                                                               gCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATgaggag                                                                     <  3:722989/151‑1 (MQ=255)
                                                                               gCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATgaggag                                                                     <  8:1296568/151‑1 (MQ=255)
                                                                               gCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATgagga                                                                      <  6:1146333/150‑1 (MQ=255)
                                                                                ccACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATgagg                                                                       >  5:2028089/1‑148 (MQ=255)
                                                                                   cacaacacaaCAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCa                                                                 <  5:331126/151‑1 (MQ=255)
                                                                                     caacacaacaAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCAtt                                                               >  5:1498208/1‑151 (MQ=255)
                                                                                       acacaacaAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTcc                                                             <  8:2109878/151‑1 (MQ=255)
                                                                                        cacaacaAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCt                                                            <  5:482991/151‑1 (MQ=255)
                                                                                        cacaacaAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCt                                                            >  1:46914/1‑151 (MQ=255)
                                                                                         acaacaCAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCtt                                                           <  7:1082735/151‑1 (MQ=255)
                                                                                          caacaaAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTa                                                          <  8:1775002/151‑1 (MQ=255)
                                                                                            acaaAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACg                                                        <  1:796469/151‑1 (MQ=255)
                                                                                            acaaAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTACATACCGATGAGGAGATCATTCCTTACg                                                        <  4:450303/151‑1 (MQ=255)
                                                                                              aaaGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAg                                                      <  4:2125831/151‑1 (MQ=255)
                                                                                               aaGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAgt                                                     >  4:1928974/1‑151 (MQ=255)
                                                                                                aGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAgtg                                                    >  8:1919651/1‑151 (MQ=255)
                                                                                                  cGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGtgtg                                                  >  3:447669/1‑151 (MQ=255)
                                                                                                      gCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACgg                                              <  4:977115/151‑1 (MQ=255)
                                                                                                          aCTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTg                                          >  3:1031448/1‑151 (MQ=255)
                                                                                                          aCTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTg                                          >  5:1928859/1‑151 (MQ=255)
                                                                                                            tCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCCCTGCGTGAGCATGTCCCTGGACTTGAGCTCCTGCATACCGATGAGGAGCTCAGGCCTTACGAGTGTGCCGGGTTgcg                                        >  2:1957580/1‑149 (MQ=255)
                                                                                                            tCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTg                                          >  3:94288/1‑149 (MQ=255)
                                                                                                             cATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGa                                         <  2:104583/149‑1 (MQ=255)
                                                                                                             cATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGa                                         <  7:1593048/149‑1 (MQ=255)
                                                                                                             cATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCAATGCGTGAGCATGTACCTGGACTTGAGAACATGCATACGGAGGAGGAGATCATTCATTACCAGAGTGACGGGTTGAgc                                       >  3:180770/1‑151 (MQ=255)
                                                                                                              aTGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGAGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAgcg                                      >  8:380938/1‑151 (MQ=255)
                                                                                                              aTGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAgcg                                      <  4:176335/151‑1 (MQ=255)
                                                                                                                gAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGcgcg                                    <  3:2012850/151‑1 (MQ=255)
                                                                                                                 aGCATCTTGTACGAACAGAGTCTTGAAGGCAAATAACTCTAACTCGACAGCACATCGGTACTGAAGGCAATGCGTGAGCATATCCCTCGAATTGAGATCCTGAATACCGAACAGCAGATCATTCCTTACGAGTGTGACGGCTTGacctcg                                    >  4:1406888/1‑145 (MQ=255)
                                                                                                                   cATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTAt                                 >  6:176868/1‑151 (MQ=255)
                                                                                                                         gTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCAcg                           >  8:1105085/1‑151 (MQ=255)
                                                                                                                          tACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCAcgc                          >  4:1508247/1‑151 (MQ=255)
                                                                                                                           aCGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCAcgc                          <  7:738076/150‑1 (MQ=255)
                                                                                                                            cGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGCACATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCACGCGt                        >  2:819086/1‑151 (MQ=255)
                                                                                                                               agagCGTCTTGATGGCGCTTTACTCGATTGCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCACGCGTCCa                     <  4:815663/151‑1 (MQ=255)
                                                                                                                                 agCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCACGCGTCCAtt                   <  7:477141/151‑1 (MQ=255)
                                                                                                                                    gTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCACGCGTCCATTACt                >  7:1958724/1‑151 (MQ=255)
                                                                                                                                        tGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCACGCGTCCATTACTg               <  6:271449/148‑1 (MQ=255)
                                                                                                                                         gATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCACGCGTCCATTACTGgtt            <  7:393229/150‑1 (MQ=255)
                                                                                                                                                cTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCACGCGTCCATTACTGGTTGTTCTGcc    >  8:1534754/1‑151 (MQ=255)
                                                                                                                                                  ttACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCACGCGTCCATTACTGGTTGTTCTGCCt   <  6:140878/150‑1 (MQ=255)
                                                                                                                                                  ttACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCACGCGTCCATTACTGGTTGTTCTGCCt   <  6:1050627/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
GACCGGTAGCTAAAGAGATAGACGAAAACGAAAAGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGCGAAGCCTACTCATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACTCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCACGCGTCCATTACTGGTTGTTCTGCCTA  >  DH1/755632‑755928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑