Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,743,5580TC59.5% 371.5 / inf 554V385V (GTT→GTC) wzctyrosine‑protein kinase wzc
Reads supporting (aligned to +/- strand):  ref base T (106/118);  new base C (176/153);  total (283/271)
Fisher's exact test for biased strand distribution p-value = 1.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.03e-01

Only 100 of 557 total aligned reads displayed.

CAAAGTTCACCCGGCGTACCGCACGCTGCTGGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATCCTCGGCGCGATTATCCTTGG  >  DH1/1743408‑1743707
                                                                                                                                                      |                                                                                                                                                     
   aGTTCACCCGGCGTACCGCACGCTGCTGGAGAACCGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCtat                                                                                                                                                    >  4:464070/1‑151 (MQ=255)
   aGTTCACCCGGCGTACCGCACGCTGCTGGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCtat                                                                                                                                                    >  6:1280302/1‑151 (MQ=255)
          ccGGCGTACCGCACGCTGCTGGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAAc                                                                                                                                             <  1:1123763/151‑1 (MQ=255)
           cGGCGTACCGCACGCTGCTGGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAAct                                                                                                                                            >  5:1745465/1‑151 (MQ=255)
            ggCGTACCGCACGCTGCTGGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAActg                                                                                                                                           <  8:1868188/151‑1 (MQ=255)
            ggCGTACCGCACGCTGCTGGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAActg                                                                                                                                           <  5:365703/151‑1 (MQ=255)
                 aCCGCACGCTGCTGGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGATAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGaa                                                                                                                                      <  8:1609508/151‑1 (MQ=255)
                 aCCGCACGCTGCTGGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGaa                                                                                                                                      <  8:243169/151‑1 (MQ=255)
                 aCCGCACGCTGCTGGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGa                                                                                                                                       >  1:1861064/1‑150 (MQ=255)
                   cGCACGCTGCTGGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATa                                                                                                                                    <  3:298348/151‑1 (MQ=255)
                   cGCACGCTGCTGGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAAt                                                                                                                                     >  3:782056/1‑150 (MQ=255)
                    gCACGCTGCTGGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATaa                                                                                                                                   <  6:2239410/151‑1 (MQ=255)
                        gctgctGGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAagag                                                                                                                               <  7:279967/151‑1 (MQ=255)
                         ctgctgGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGc                                                                                                                              >  5:1512793/1‑151 (MQ=255)
                           gctgGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGc                                                                                                                              >  3:1016818/1‑149 (MQ=255)
                              ggAGAAACGTGAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAg                                                                                                                         <  1:2203158/151‑1 (MQ=255)
                              ggAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGa                                                                                                                          <  1:380527/150‑1 (MQ=255)
                               gagaAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGc                                                                                                                        <  2:1895113/151‑1 (MQ=255)
                                  aaaCGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGa                                                                                                                     <  6:723954/151‑1 (MQ=255)
                                  aaaCGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGa                                                                                                                     <  7:2237403/151‑1 (MQ=255)
                                   aaCGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGCTGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGa                                                                                                                     >  7:175659/1‑150 (MQ=255)
                                   aaCGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGa                                                                                                                     >  1:898906/1‑150 (MQ=255)
                                    aCGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGaaa                                                                                                                   <  4:5482/151‑1 (MQ=255)
                                         aGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCCGCAGGAGATTGTCCGTCTGCCCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATccc                                                                                                              >  4:1374786/1‑149 (MQ=255)
                                         aGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCAc                                                                                                              >  1:2129063/1‑151 (MQ=255)
                                               tGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGc                                                                                                        >  4:790794/1‑151 (MQ=255)
                                                ggAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGcc                                                                                                       >  7:828547/1‑151 (MQ=255)
                                                ggAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGc                                                                                                        <  8:487210/150‑1 (MQ=255)
                                                 gAAGAGGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCGGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCa                                                                                                      <  2:1535193/151‑1 (MQ=255)
                                                 gAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCa                                                                                                      <  1:512182/151‑1 (MQ=255)
                                                       gAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGAAGGAGCTGAAAATCACCGAGGCCAGCACCg                                                                                                >  3:1859225/1‑151 (MQ=255)
                                                        aaaaaGCCATACTTAACGGTCGGGTAACGGCGATGCCGAAAAGCCTGGAGGAGATTGTCCGTCTGAGCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGGTGAAAATCACCGAGGCCAGCACCGt                                                                                               <  6:1675143/151‑1 (MQ=255)
                                                        aaaaaGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCCCCGAGGCCAGCACCgg                                                                                               >  8:883557/1‑150 (MQ=255)
                                                         aaaaGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTc                                                                                              <  5:966215/151‑1 (MQ=255)
                                                         aaaaGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTc                                                                                              <  7:986095/151‑1 (MQ=255)
                                                         aaaaGCCAAACTTAACGGTCCCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTc                                                                                              <  8:40699/151‑1 (MQ=255)
                                                          aaaGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCg                                                                                             <  5:48080/151‑1 (MQ=255)
                                                          aaaGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCg                                                                                             >  2:822257/1‑151 (MQ=255)
                                                              ccAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCg                                                                                          >  7:2050680/1‑150 (MQ=255)
                                                                aaaCTTAACGGTCGAGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGAtg                                                                                       <  6:1950515/151‑1 (MQ=255)
                                                                 aaCTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGAtgt                                                                                      >  1:1009812/1‑151 (MQ=255)
                                                                  aCTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGAtgtg                                                                                     >  4:1524954/1‑151 (MQ=255)
                                                                   cTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGAtgtgg                                                                                    >  3:2095629/1‑150 (MQ=255)
                                                                      aaCGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCCGCGATGTgcgc                                                                                  >  4:2198592/1‑150 (MQ=255)
                                                                      aaCGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGGCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTgcgc                                                                                  <  7:2194374/150‑1 (MQ=255)
                                                                       aCGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCAt                                                                                <  6:959070/151‑1 (MQ=255)
                                                                         ggTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCAttg                                                                              >  2:1050807/1‑151 (MQ=255)
                                                                            cgcgTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCAttgttg                                                                           >  3:141824/1‑151 (MQ=255)
                                                                             gcgTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCAttgttg                                                                           >  7:614832/1‑150 (MQ=255)
                                                                              cgTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGAc                                                                         <  6:1025062/151‑1 (MQ=255)
                                                                                  aCGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCgg                                                                     >  8:1375871/1‑151 (MQ=255)
                                                                                   cGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGc                                                                    >  3:1530186/1‑151 (MQ=255)
                                                                                   cGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGc                                                                    >  5:678452/1‑151 (MQ=255)
                                                                                     gCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCa                                                                   >  6:510096/1‑150 (MQ=255)
                                                                                     gCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCa                                                                   >  1:2034321/1‑150 (MQ=255)
                                                                                       gATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATc                                                                <  2:1864650/151‑1 (MQ=255)
                                                                                        aTGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATc                                                                >  3:648361/1‑150 (MQ=255)
                                                                                          gCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCAc                                                              >  2:1746706/1‑150 (MQ=255)
                                                                                             gAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGCCCCGGCAATCACTc                                                            >  5:2165033/1‑149 (MQ=255)
                                                                                             gAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAg                                                          <  3:465587/151‑1 (MQ=255)
                                                                                                  cccAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTgg                                                     <  6:1758247/151‑1 (MQ=255)
                                                                                                   ccAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGc                                                    >  5:1126928/1‑151 (MQ=255)
                                                                                                           agaTTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCAttgtt                                                                            <  7:1141131/119‑1 (MQ=255)
                                                                                                             aTTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAAc                                          <  6:2066606/151‑1 (MQ=255)
                                                                                                             aTTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAAc                                          >  1:2083169/1‑151 (MQ=255)
                                                                                                              ttGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAAcc                                         >  2:561909/1‑151 (MQ=255)
                                                                                                               tGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAAcc                                         <  8:750107/150‑1 (MQ=255)
                                                                                                               tGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCg                                        >  4:1277107/1‑151 (MQ=255)
                                                                                                               tGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGCCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAAcc                                         <  4:179084/150‑1 (MQ=255)
                                                                                                                gTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCg                                        <  7:1310340/150‑1 (MQ=255)
                                                                                                                 tCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGTAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCga                                       >  8:583141/1‑150 (MQ=255)
                                                                                                                 tCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCga                                       >  4:315624/1‑150 (MQ=255)
                                                                                                                 tCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGGCTATATGCAACTCCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCCATGTCCGCATTGTAGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCga                                       >  6:1532350/1‑150 (MQ=255)
                                                                                                                   cGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTGTATATGCAACTGCTGAATAAAGAGCAGGAGATGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCga                                       >  5:1414034/1‑148 (MQ=255)
                                                                                                                      gtGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAg                                 <  4:971544/150‑1 (MQ=255)
                                                                                                                      cTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCGGAAACCgaagaa                                   >  5:249162/1‑149 (MQ=255)
                                                                                                                       tGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAgg                                >  1:687972/1‑151 (MQ=255)
                                                                                                                        gACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAgg                                <  1:1551538/150‑1 (MQ=255)
                                                                                                                        gACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAggg                               <  2:1389212/151‑1 (MQ=255)
                                                                                                                          cccGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCt                             <  1:13099/151‑1 (MQ=255)
                                                                                                                             gcgATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGAt                          >  4:10342/1‑151 (MQ=255)
                                                                                                                              cgATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGAtt                         <  8:2040048/151‑1 (MQ=255)
                                                                                                                              cgATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGAtt                         <  1:1734585/151‑1 (MQ=255)
                                                                                                                                  gTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATcc                     <  2:2191801/151‑1 (MQ=255)
                                                                                                                                  gTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATcc                     >  7:388918/1‑151 (MQ=255)
                                                                                                                                   tCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATCCt                    >  5:12772/1‑151 (MQ=255)
                                                                                                                                    cGAGTCTTGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTTAAAATCACCTAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCTGCAATCACTCAGCCTTGCGTGCTGAAACCGAAGAAAGGGCTGATTATCCTc                   <  6:117090/151‑1 (MQ=255)
                                                                                                                                     gAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATCCTCg                  <  3:1277626/151‑1 (MQ=255)
                                                                                                                                     gAGTCTGGTCAGCAGGGCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATCCTCg                  <  2:408430/151‑1 (MQ=255)
                                                                                                                                      aGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATCCTCgg                 <  3:1673177/151‑1 (MQ=255)
                                                                                                                                       gTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATCCTCGgc                <  7:1575689/151‑1 (MQ=255)
                                                                                                                                       gTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATCCTCGgc                >  4:694679/1‑151 (MQ=255)
                                                                                                                                         cTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATCCTCGgcgc              >  8:1477323/1‑151 (MQ=255)
                                                                                                                                           ggTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCGGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGCCCCGGCAATCACTCAGCCTCGCGTGCTGAAACCGAAGAAAGGGCTGATAATCCTCGGcgcg             >  7:1052175/1‑150 (MQ=255)
                                                                                                                                            gTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATCCTCGGCGCGAt           <  5:1931039/151‑1 (MQ=255)
                                                                                                                                             tCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATCCTCGGCGCGAtt          >  1:1924844/1‑151 (MQ=255)
                                                                                                                                                 cagGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATCCTCGGCGCGATTATcc      <  7:1959086/151‑1 (MQ=255)
                                                                                                                                                 cagGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATCCTCGGCGCGATTATcc      >  7:1467814/1‑151 (MQ=255)
                                                                                                                                                  agGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATCCTCGGCGCGATTATCCt     <  2:1232329/151‑1 (MQ=255)
                                                                                                                                                   ggTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATCCTCGGCGCGATTATCCtt    >  8:1229552/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CAAAGTTCACCCGGCGTACCGCACGCTGCTGGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTTTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAGGCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTGAAACCGAAGAAAGGGCTGATTATCCTCGGCGCGATTATCCTTGG  >  DH1/1743408‑1743707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑