Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,234,7990AT55.8% 172.6 / inf 373Q209L (CAG→CTG) ydfUhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (74/91);  new base T (108/100);  total (182/191)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 374 total aligned reads displayed.

AATTATCTTTGGCTGAATTATCCTGGTGGGCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCTTTCATGAAGCTGCCTAAACG  >  DH1/2234652‑2234949
                                                                                                                                                   |                                                                                                                                                      
 aTTATCTTTGGCTGAATTATCCTGGTGGGCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGAcc                                                                                                                                                    <  7:1759894/151‑1 (MQ=255)
  ttATCTTTGGCTGAATTATCCTGGTGGGCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGCTGTACCGCGAAAGCGACATCGTACCGCGAGAGCTGACCg                                                                                                                                                   >  2:304884/1‑151 (MQ=255)
   tATCTTTGGCTGAATTATCCTGGTGGGCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGc                                                                                                                                                  >  7:1849173/1‑151 (MQ=255)
       tttGGCTGAATTATCCTGGTGGGCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGCGCTGACCGccccc                                                                                                                                              >  6:51032/1‑151 (MQ=255)
         tGGCTGAATTATCCTGGTGGGCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGccacca                                                                                                                                             <  6:242891/150‑1 (MQ=255)
          ggCTGAATTATCCTGGTGGGCGGTCTGCAATCCGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGc                                                                                                                                           <  5:1158869/151‑1 (MQ=255)
          ggCTGAATTATCCTGGTGGGCGGTCTGCAATCAGGTGGTGGATGCGCTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGCGCAGACCGCCACCAGc                                                                                                                                           >  1:133341/1‑151 (MQ=255)
              gAATTATCCTGGTGGGCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCat                                                                                                                                         <  2:1864140/149‑1 (MQ=255)
               aaTTATCCTGGTGGGCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATAtt                                                                                                                                      <  7:370430/151‑1 (MQ=255)
                aTTATCCTGGTGGGCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCCCCAGCATAttt                                                                                                                                     >  7:472905/1‑150 (MQ=255)
                aTTATCCTGGTGGGCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTg                                                                                                                                     <  3:856185/151‑1 (MQ=255)
                 ttATCCTGGTGGGCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTg                                                                                                                                     >  5:99159/1‑150 (MQ=255)
                   aTCCTGGTGGGCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGaa                                                                                                                                   >  8:1445971/1‑150 (MQ=255)
                     ccTGGTGGGCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAaaca                                                                                                                                <  5:386269/151‑1 (MQ=255)
                        ggtggGCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAaacaac                                                                                                                              <  1:449484/150‑1 (MQ=255)
                           gggCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGcac                                                                                                                          <  1:1525397/151‑1 (MQ=255)
                             gCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACaa                                                                                                                        <  5:517174/151‑1 (MQ=255)
                              cGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACaaa                                                                                                                       <  7:572138/151‑1 (MQ=255)
                               ggTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACaaaa                                                                                                                      <  6:1623504/151‑1 (MQ=255)
                                    gCAATCAGGTGGGGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCt                                                                                                                 <  1:1690905/151‑1 (MQ=255)
                                     cAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCtt                                                                                                                <  2:841877/151‑1 (MQ=255)
                                     cAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCtt                                                                                                                <  1:1861735/151‑1 (MQ=255)
                                       aTCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGc                                                                                                              <  8:1728297/151‑1 (MQ=255)
                                       aTCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGc                                                                                                              <  4:1152335/151‑1 (MQ=255)
                                       aTCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGACTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGc                                                                                                              <  1:145987/151‑1 (MQ=255)
                                       aTCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCATTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGc                                                                                                              <  7:1967705/151‑1 (MQ=255)
                                         cAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCa                                                                                                             <  4:1331225/150‑1 (MQ=255)
                                          aGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCAcc                                                                                                           <  5:1428304/151‑1 (MQ=255)
                                          aGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCAcc                                                                                                           <  7:873670/151‑1 (MQ=255)
                                           ggtggtGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCg                                                                                                          <  5:1334139/151‑1 (MQ=255)
                                             tcgtggATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACACCGAAAGCGACATCGTACCCGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCATGCAACGt                                                                                                         >  2:955977/3‑150 (MQ=255)
                                             gggtggATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGtt                                                                                                        <  5:791616/150‑1 (MQ=255)
                                              ggttgATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCTTTg                                                                                                       <  8:1622578/151‑1 (MQ=255)
                                              ggtggATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGtt                                                                                                        <  1:1160460/150‑1 (MQ=255)
                                               gtggATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAGAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGc                                                                                                      >  3:268686/1‑151 (MQ=255)
                                                tggATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGcc                                                                                                     >  5:253107/1‑151 (MQ=255)
                                                 ggATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGccc                                                                                                    >  3:1556366/1‑150 (MQ=255)
                                                   aTGCACTACCTGAGGGTGTATCGCGGCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTa                                                                                                  <  4:397592/151‑1 (MQ=255)
                                                   aTGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCtt                                                                                                   >  3:723626/1‑150 (MQ=255)
                                                   aTGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTa                                                                                                  <  2:1389134/151‑1 (MQ=255)
                                                     gCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTAc                                                                                                 <  8:898614/150‑1 (MQ=255)
                                                     gCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTAc                                                                                                 <  5:1902841/150‑1 (MQ=255)
                                                          aCCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCccac                                                                                           >  2:147375/1‑151 (MQ=255)
                                                          aCCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCccac                                                                                           >  4:789295/1‑151 (MQ=255)
                                                             tGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCccacc                                                                                          >  4:1562828/1‑149 (MQ=255)
                                                              gAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAACGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGACACAACGCACAACAAATCTTGCACCGTTGCCTTACGCCCACCAGc                                                                                       >  6:1470401/1‑151 (MQ=255)
                                                              gAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCccacca                                                                                         <  7:1581384/149‑1 (MQ=255)
                                                              gAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAg                                                                                        <  8:1489741/150‑1 (MQ=255)
                                                              gAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGc                                                                                       <  6:338439/151‑1 (MQ=255)
                                                               aGGCGGTATCGCGTCGTTCCCAGGCATTACCAGCGGAAAAAAAATGCTCTGTGGACCGAGAAAGCGACATCGTACCGGGAGAGCTGCCCGCCACCCGCATATTGCAACAACGCACAAAAAATAGTCCACCTTTCCCTGACCCccaccaccc                                                                                      >  8:1124857/1‑148 (MQ=255)
                                                                 gCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGcaac                                                                                    >  2:1739646/1‑151 (MQ=255)
                                                                      aTCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACaaaa                                                                                >  3:1811808/1‑150 (MQ=255)
                                                                      aTCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACaaaa                                                                                >  5:1113434/1‑150 (MQ=255)
                                                                         gcgTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATca                                                                            <  3:542798/151‑1 (MQ=255)
                                                                          cgtcgtTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATcac                                                                           >  3:664494/1‑151 (MQ=255)
                                                                             cgtTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATcaccac                                                                        >  8:592888/1‑151 (MQ=255)
                                                                              gtTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACcaca                                                                       <  4:554704/151‑1 (MQ=255)
                                                                                    tGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGaaa                                                                  >  2:438289/1‑150 (MQ=255)
                                                                                     gggATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGaaa                                                                  >  2:1195435/1‑149 (MQ=255)
                                                                                     gggATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGaaa                                                                  <  7:1132715/149‑1 (MQ=255)
                                                                                          tACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACgg                                                            <  6:843584/150‑1 (MQ=255)
                                                                                           aCCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACggtg                                                          <  7:960015/151‑1 (MQ=255)
                                                                                           aCCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACggtg                                                          >  3:1893235/1‑151 (MQ=255)
                                                                                            ccAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACggtgg                                                         >  3:1349528/1‑151 (MQ=255)
                                                                                              aGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTa                                                       <  1:304852/151‑1 (MQ=255)
                                                                                               gCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTaa                                                      <  8:330266/151‑1 (MQ=255)
                                                                                                 ggAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGc                                                    >  3:32273/1‑151 (MQ=255)
                                                                                                  gAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCa                                                   <  6:1221610/151‑1 (MQ=255)
                                                                                                   aaaaaaTCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCa                                                   <  8:687316/150‑1 (MQ=255)
                                                                                                       aaTCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGCTGGAAAGCATCAcc                                              >  8:746950/1‑151 (MQ=255)
                                                                                                         tCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGt                                            >  6:1266488/1‑151 (MQ=255)
                                                                                                           tGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTg                                          >  8:1087280/1‑151 (MQ=255)
                                                                                                            gCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGa                                         <  3:963096/151‑1 (MQ=255)
                                                                                                                ggTGTACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATcc                                      >  1:532847/1‑150 (MQ=255)
                                                                                                                  tgtACCGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATcc                                      >  2:1598529/1‑148 (MQ=255)
                                                                                                                    tACCGCGAAAGCGACATCGTACCGCGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCagag                                  <  2:1101303/150‑1 (MQ=255)
                                                                                                                     aCCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGt                                 <  6:1794931/150‑1 (MQ=255)
                                                                                                                      ccGCGAAAGCGACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGtct                               >  4:1811246/1‑151 (MQ=255)
                                                                                                                          gAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCgg                           <  3:1132077/151‑1 (MQ=255)
                                                                                                                            aaGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCCCAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGa                          >  4:33443/1‑150 (MQ=255)
                                                                                                                            aaGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGa                          >  1:683956/1‑150 (MQ=255)
                                                                                                                               ggACATCGTACCGGGAGAGCTGACCGACACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCt                      <  6:1726842/150‑1 (MQ=255)
                                                                                                                                gACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCtt                     >  7:1396635/1‑151 (MQ=255)
                                                                                                                                gACATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCtt                     <  3:126438/151‑1 (MQ=255)
                                                                                                                                 aCATCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCGTCACCGTTGATCCAGAGTCTCCGGAATCttt                    <  5:1697927/151‑1 (MQ=255)
                                                                                                                                   aTCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCTTTc                   >  7:737116/1‑150 (MQ=255)
                                                                                                                                   aTCGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCTTTCa                  <  7:659147/151‑1 (MQ=255)
                                                                                                                                   aTCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGAATCACCGTTGATCCAGACTCTACGGAATCTTTc                   >  4:49993/1‑150 (MQ=255)
                                                                                                                                     cGTACCGGGAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCTTTCATg                >  3:100626/1‑151 (MQ=255)
                                                                                                                                        aCCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCTTTCATGAAg             <  3:906915/151‑1 (MQ=255)
                                                                                                                                           gggAGAGCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCTTTCATGAAGCTg          >  1:889765/1‑151 (MQ=255)
                                                                                                                                           gggAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCTTTCATGAAGCTg          <  7:1260276/151‑1 (MQ=255)
                                                                                                                                           gggAGAGCAGACCGCCACCAGCATATTGAAACAAAGCACAAAAAATCTTGCACCGTTGCCTTACGCCAACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCTTTCATGAAGCTg          >  5:484885/1‑151 (MQ=255)
                                                                                                                                             gagaGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCAGAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCTTTCATGAAGCTGcc        >  8:1292811/1‑151 (MQ=255)
                                                                                                                                             gagaGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCTTTCATGAAGCTGcc        >  5:63302/1‑151 (MQ=255)
                                                                                                                                              agagCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCTTTCATGAAGCTGCCt       >  2:196980/1‑151 (MQ=255)
                                                                                                                                              agagCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCTTTCATGAAGCTGCCt       <  7:1058565/151‑1 (MQ=255)
                                                                                                                                              agagCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGGTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCTTTCATGAAGCTGCCt       <  2:721248/151‑1 (MQ=255)
                                                                                                                                               gagCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCTTTCATGAAGCTGCCt       >  7:311623/1‑150 (MQ=255)
                                                                                                                                                agCTGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCTTTCATGAAGCTGCCTaa     <  3:171865/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
AATTATCTTTGGCTGAATTATCCTGGTGGGCGGTCTGCAATCAGGTGGTGGATGCACTACCTGAGGCTGTATCGCGTCGTTCGCTGGGATTACCAGCGGAAAAAATCTGCTCGGTGTACCGCGAAAGCGACATCGTACCGGGAGAGCAGACCGCCACCAGCATATTGAAACAACGCACAAAAAATCTTGCACCGTTGCCTTACGCCCACCAGCAACAAAAATCACCACAGGAAAAGACGGTGGTAAGCATCACCGTTGATCCAGAGTCTCCGGAATCTTTCATGAAGCTGCCTAAACG  >  DH1/2234652‑2234949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑