Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,520,8180GA56.8% 176.6 / inf 348C110Y (TGT→TAT) puuPputrescine importer
Reads supporting (aligned to +/- strand):  ref base G (76/73);  new base A (87/109);  total (163/185)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

Only 100 of 349 total aligned reads displayed.

GCTGGCGCTGGCGGGTGTGCTGTTTACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCGTCAACCTGGTCGCTAACTTCA  >  DH1/2520669‑2520967
                                                                                                                                                     |                                                                                                                                                     
gctggcgctggcGGGTGTGCTGTTTACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGt                                                                                                                                                      >  7:8880/1‑151 (MQ=255)
 ctggcgctggcgGGTGTGCTGTTTACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCCCGTCGGATGTATGGTCGGCTGGTCATCGCTGCTGGATTGtg                                                                                                                                                     >  2:438245/1‑150 (MQ=255)
   ggcgctggcgGGTGTGCTGTTTACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTAtct                                                                                                                                                    <  4:1097807/150‑1 (MQ=255)
   ggcgctggcgGGTGTGCTGTTTACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTAtc                                                                                                                                                     <  2:244077/149‑1 (MQ=255)
        tggcgGGTGTGCTGTTTACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCttttt                                                                                                                                              >  2:1361401/1‑151 (MQ=255)
          gcgGGTGTGCTGTTTACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGc                                                                                                                                            >  4:679859/1‑151 (MQ=255)
               tgtgCTGTTTACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCgatg                                                                                                                                       >  6:1308678/1‑151 (MQ=255)
                     gTTTACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTaa                                                                                                                                  <  2:1208021/150‑1 (MQ=255)
                     gTTTACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAAc                                                                                                                                 <  7:1396624/151‑1 (MQ=255)
                       ttACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGt                                                                                                                               >  7:222055/1‑151 (MQ=255)
                       ttACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGt                                                                                                                               >  2:1721301/1‑151 (MQ=255)
                         aCCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTc                                                                                                                              <  2:1881573/150‑1 (MQ=255)
                         aCCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCt                                                                                                                             >  1:1839741/1‑151 (MQ=255)
                          ccGCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCtt                                                                                                                            >  2:884040/1‑151 (MQ=255)
                           ggCTATCAGCTACGGCAAACTGGTGCGCGAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCttg                                                                                                                           <  7:39416/150‑1 (MQ=255)
                            gCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCttgt                                                                                                                          <  5:453427/151‑1 (MQ=255)
                            gCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCttgt                                                                                                                          <  7:155981/151‑1 (MQ=255)
                            gCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTATTTGCCGATGATTAACGTCttgt                                                                                                                          >  7:119518/1‑151 (MQ=255)
                              tATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCttgttg                                                                                                                        >  3:1028936/1‑151 (MQ=255)
                               aTCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTgg                                                                                                                       <  5:1784570/151‑1 (MQ=255)
                                 cAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCGGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGGCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCg                                                                                                                     <  5:105862/151‑1 (MQ=255)
                                 cAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGc                                                                                                                      <  4:1654494/150‑1 (MQ=255)
                                 cAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCg                                                                                                                     <  4:1380390/151‑1 (MQ=255)
                                  aGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATCCCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATAATCTCTTTTTGACGATGATTAACGTCTGGTTGGCg                                                                                                                     >  2:102440/1‑150 (MQ=255)
                                  aGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAACGTCGATTAACCCGCACGGCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGa                                                                                                                    <  6:1094505/151‑1 (MQ=255)
                                      aCGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAat                                                                                                                <  2:639502/151‑1 (MQ=255)
                                      aCGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTCTGCCGATGATTAACGTCTTGTTGGCGaaaa                                                                                                                 <  3:429612/150‑1 (MQ=255)
                                      aCGGAAAACTGGTTCGCCAGTTTCCGGAGGCCGGATCGGCCTATACCTACGCTCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCTATGATTAACGTCTTGTTGGCGAAAat                                                                                                                <  8:1041106/151‑1 (MQ=255)
                                       cGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAatc                                                                                                               <  5:150494/151‑1 (MQ=255)
                                        ggCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAatct                                                                                                              <  4:1819974/151‑1 (MQ=255)
                                          cAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAatctat                                                                                                            >  6:163870/1‑151 (MQ=255)
                                           aaaCTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTTGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAatctatc                                                                                                           <  1:1480582/151‑1 (MQ=255)
                                           aaaCTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAatctatc                                                                                                           >  4:1772643/1‑151 (MQ=255)
                                                ggTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTcc                                                                                                      >  3:328502/1‑151 (MQ=255)
                                                 gTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCg                                                                                                     <  5:1368552/151‑1 (MQ=255)
                                                   tCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGc                                                                                                    <  6:640976/150‑1 (MQ=255)
                                                    cGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGccc                                                                                                  <  3:1678427/151‑1 (MQ=255)
                                                      ccAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCctc                                                                                                <  2:1301478/151‑1 (MQ=255)
                                                       cAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCctct                                                                                               <  2:1956558/151‑1 (MQ=255)
                                                        aGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGTGAAAATCTATCTCTCCGCCCTCtt                                                                                              <  8:1119600/151‑1 (MQ=255)
                                                        aGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCtt                                                                                              >  8:1454148/1‑151 (MQ=255)
                                                        aGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCtt                                                                                              >  4:554368/1‑151 (MQ=255)
                                                        aGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCtt                                                                                              <  4:887073/151‑1 (MQ=255)
                                                        aGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCtt                                                                                              <  6:1817237/151‑1 (MQ=255)
                                                        aGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCtt                                                                                              >  7:1756493/1‑151 (MQ=255)
                                                         gTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTc                                                                                             >  8:974746/1‑151 (MQ=255)
                                                          tttCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTcc                                                                                            >  2:646471/1‑151 (MQ=255)
                                                             ccGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTTGCGAAAATCTATCTCTCCGCCCTCTTCCCgg                                                                                         >  3:544650/1‑151 (MQ=255)
                                                                gAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCCTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAg                                                                                      <  3:625938/151‑1 (MQ=255)
                                                                 aGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGt                                                                                     <  6:1410983/151‑1 (MQ=255)
                                                                 aGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGt                                                                                     <  1:431085/151‑1 (MQ=255)
                                                                   gCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTgc                                                                                   <  3:1770138/151‑1 (MQ=255)
                                                                         tCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGccgccg                                                                              <  8:310156/150‑1 (MQ=255)
                                                                         tCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCgt                                                                             >  7:1684484/1‑151 (MQ=255)
                                                                              cTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCgtgggtg                                                                        >  2:1530836/1‑151 (MQ=255)
                                                                                  aCCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCgtgggtgtggg                                                                    >  8:181511/1‑151 (MQ=255)
                                                                                  aCCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCATGTTGGCGAAAATCTATATCTCCGCCATCTTCCCGCAACTGCCGCCgagggtgtcgg                                                                    >  1:144281/1‑151 (MQ=255)
                                                                                   ccTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCgtgggtgtgggt                                                                   >  8:20605/1‑151 (MQ=255)
                                                                                   ccTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCgtgggtgtgggt                                                                   <  5:270863/151‑1 (MQ=255)
                                                                                        gcgcAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTaa                                                              <  2:810566/151‑1 (MQ=255)
                                                                                          gcAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAAcc                                                            <  4:1504118/151‑1 (MQ=255)
                                                                                          ccAAAAGTCGATTAACCCGCAAGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAAcc                                                            <  6:1780700/150‑1 (MQ=255)
                                                                                           cAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCt                                                           >  8:464920/1‑151 (MQ=255)
                                                                                            aaacGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGAGTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGGGGTAACCtt                                                          <  8:1661679/151‑1 (MQ=255)
                                                                                            aaaaGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCtt                                                          >  5:908347/1‑151 (MQ=255)
                                                                                             aaaGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTtc                                                         >  5:384196/1‑151 (MQ=255)
                                                                                               aGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCCGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTtcgt                                                       <  5:759017/151‑1 (MQ=255)
                                                                                                    aTTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCa                                                  <  4:458332/151‑1 (MQ=255)
                                                                                                    aTTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTCTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCa                                                  <  6:1546198/151‑1 (MQ=255)
                                                                                                      tAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCAt                                                 <  5:1249847/150‑1 (MQ=255)
                                                                                                       aaCCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTAGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGCAAGTGCCGCCGTGGCTCTGGGTGGTAACCTTCGTCGCCAgtt                                               >  6:962875/1‑151 (MQ=255)
                                                                                                       aaCCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCAtt                                                <  1:1306898/150‑1 (MQ=255)
                                                                                                        aCCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCAtttt                                              <  5:1026255/151‑1 (MQ=255)
                                                                                                        aCCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCGGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCAtttt                                              >  6:758165/1‑151 (MQ=255)
                                                                                                         cccGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTa                                             <  3:1379546/151‑1 (MQ=255)
                                                                                                         cccGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTACCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCAtttt                                              >  8:1232596/1‑150 (MQ=255)
                                                                                                            gCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAAcc                                          >  2:954957/1‑151 (MQ=255)
                                                                                                                gTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCGGCCGTGCGTGTGGGTGGTAACCTTCCTCGCCATTTTAAccgccg                                      >  8:1365790/1‑151 (MQ=255)
                                                                                                                   ggATTTATGGTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGTCGAAAATCTATCTCTGCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGTGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGa                                   <  5:1876918/151‑1 (MQ=255)
                                                                                                                    gATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGaa                                  <  1:894844/151‑1 (MQ=255)
                                                                                                                        tATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCAGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCGTCTTGCCGGAACTGCCGCGTTGGTTGTGTTTGGTAAGCTTGGTCGCCATTTTAACCGCCGCGAATCt                               <  2:336928/150‑1 (MQ=255)
                                                                                                                           ggTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGaa                            <  8:63307/150‑1 (MQ=255)
                                                                                                                           ggTCGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCAATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAag                           <  1:1040605/151‑1 (MQ=255)
                                                                                                                              cGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAagag                         <  3:281137/150‑1 (MQ=255)
                                                                                                                              cGGCTGGTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGc                        >  1:127027/1‑151 (MQ=255)
                                                                                                                               ggCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCg                       >  6:503464/1‑151 (MQ=255)
                                                                                                                                  tGGTGATCGCTTCTGGATTGTCTCTTTTTGCCGATGATTAAGGTCTTGTTGGCGATAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCGATTTTAACCGCCGCGAATCTGAAGAGCGTc                     <  6:1972024/150‑1 (MQ=255)
                                                                                                                                  tGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCGTc                     >  3:833079/1‑150 (MQ=255)
                                                                                                                                   ggTCATCGCTGCTGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCGTCa                    <  4:422714/150‑1 (MQ=255)
                                                                                                                                      cATCGCTGCTGGATTCTCTCTTTTTGCCGATGATTAACGGCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCGTCAACCt                <  6:583494/151‑1 (MQ=255)
                                                                                                                                       aTCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCGTCAACCTg               >  5:1941533/1‑151 (MQ=255)
                                                                                                                                          gctgctGGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCGTCAACCTGGTc            <  1:666284/151‑1 (MQ=255)
                                                                                                                                           ctgctgGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCGTCAACCTGGTCg           >  6:1153381/1‑151 (MQ=255)
                                                                                                                                            tgctgGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCGTCAACCTGGTCGc          >  2:1817600/1‑151 (MQ=255)
                                                                                                                                             gctgTATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGTGTGTGGGTGGTAACCTTCTTCGCCATTTTAACCGCCGCGAATCTGAAGAGCGTCAACCTGTTCGCt         <  1:584362/151‑1 (MQ=255)
                                                                                                                                              ctgGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCGTCAACCTGGTCGCt         >  6:282222/1‑150 (MQ=255)
                                                                                                                                               tgGATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGATTCTGAAGAGCGTCAACCTGGTCGCTa        >  1:1664193/1‑150 (MQ=255)
                                                                                                                                                ggATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCGGCAACCTGGTCGCTaa       <  8:780424/150‑1 (MQ=255)
                                                                                                                                                ggATTATCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCGTCAACCTGGTCGCTAAc      >  3:1278013/1‑151 (MQ=255)
                                                                                                                                                    tGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATATTAACCGCCGCGAATCTGAAGAGCGTCAACCTGGTCGCTAACTTc   >  5:1150003/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTGGCGCTGGCGGGTGTGCTGTTTACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTTCCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCTGCTGGATTGTCTCTTTTTGCCGATGATTAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGCCGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCGTCAACCTGGTCGCTAACTTCA  >  DH1/2520669‑2520967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑