BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	2,587,809	0	T	C	54.6%	106.2 / inf	367	pseudogene (428/1014 nt)	galU	response regulator of RpoS
Reads supporting (aligned to +/- strand): ref base T (74/92); new base C (87/113); total (161/206)
Fisher's exact test for biased strand distribution p-value = 9.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.67e-01

Only 100 of 370 total aligned reads displayed.

AGTGCGGCAATATCAAGCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCTTCAACGCCCAGACGTAACGCTTTGGCAATAT  >  DH1/2587659‑2587959

aGTGCGGCAATATCAAGCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCt                                                                                                                                                        >  5:1639322/1‑151 (MQ=255)

   gCGGCAATATCAAGCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAg                                                                                                                                                      <  2:280920/150‑1 (MQ=255)

       cAATATCAAGCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTcgcg                                                                                                                                                 <  2:1562855/151‑1 (MQ=255)

        aaTATCAAGCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTcgcg                                                                                                                                                 >  1:1381495/1‑150 (MQ=255)

        aaTATCAAGCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTcgcg                                                                                                                                                 >  3:1549425/1‑150 (MQ=255)

        aaTATCAAGCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGa                                                                                                                                                <  6:1731853/151‑1 (MQ=255)

        aaTATCAAGCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTcgcg                                                                                                                                                 >  7:1543374/1‑150 (MQ=255)

        aaTATCAAGCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGa                                                                                                                                                <  7:1433586/151‑1 (MQ=255)

         atatCAAGCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGaa                                                                                                                                               <  5:260397/151‑1 (MQ=255)

          aatCAAGCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGaaa                                                                                                                                              <  2:328675/150‑1 (MQ=255)

           atCAAGCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGaaaa                                                                                                                                             <  5:1078602/151‑1 (MQ=255)

             cAAGCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGaaaaa                                                                                                                                            <  1:1026308/150‑1 (MQ=255)

              aaGCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGc                                                                                                                                          >  1:1783448/1‑151 (MQ=255)

               aGCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGcc                                                                                                                                         <  6:1637489/151‑1 (MQ=255)

                gCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTATTACTTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTGGCGAAAAAGCCt                                                                                                                                        >  4:1210697/1‑151 (MQ=255)

                  aCCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGACttt                                                                                                                                      >  1:1689965/1‑151 (MQ=255)

                     aGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTttcct                                                                                                                                   >  4:1582434/1‑151 (MQ=255)

                     aGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTttcct                                                                                                                                   >  8:850041/1‑151 (MQ=255)

                      ggCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTttcctc                                                                                                                                  <  1:1502550/151‑1 (MQ=255)

                            gTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTttcctcttcctc                                                                                                                            <  1:1818385/151‑1 (MQ=255)

                             tttGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTttcctcttcctc                                                                                                                            >  6:821813/1‑150 (MQ=255)

                              ttGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCa                                                                                                                           <  7:523216/150‑1 (MQ=255)

                              ttGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCa                                                                                                                           >  1:924990/1‑150 (MQ=255)

                               tGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCaa                                                                                                                          <  1:701219/150‑1 (MQ=255)

                                 tCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAAACGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCGATTCCTCTTCCTCAAcgc                                                                                                                       >  4:1008693/1‑151 (MQ=255)

                                     cgGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAAcgcgcg                                                                                                                    >  1:1848004/1‑150 (MQ=255)

                                     cgGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAAcgcgcg                                                                                                                    >  6:440790/1‑150 (MQ=255)

                                       gCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAAt                                                                                                                 >  8:1875452/1‑151 (MQ=255)

                                        gaaCCAATTGACGATAATTATCCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCTAAAAAGCCTTTCCTCTTCCTCAACGCGCGAAtt                                                                                                                <  3:1004546/150‑1 (MQ=255)

                                         aaCCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTa                                                                                                               <  3:1159967/151‑1 (MQ=255)

                                                 gACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCt                                                                                                       >  5:326189/1‑151 (MQ=255)

                                                 gACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCt                                                                                                       <  5:494037/151‑1 (MQ=255)

                                                   cGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTgg                                                                                                     >  4:1730082/1‑151 (MQ=255)

                                                     aTAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGa                                                                                                   <  4:772873/151‑1 (MQ=255)

                                                      tAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTTGGa                                                                                                   <  7:1655758/150‑1 (MQ=255)

                                                          tAACGCGTCAATGGGAAATCACCTGCTGAAGCGGCTGTTGTAGTTCATGTAATAATTTCGACGATGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATaga                                                                                               <  4:43164/150‑1 (MQ=255)

                                                          tAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATaga                                                                                               <  4:949721/150‑1 (MQ=255)

                                                           aaCCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGCGATag                                                                                                >  6:1064256/1‑148 (MQ=255)

                                                             cccGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACa                                                                                           <  5:1446393/151‑1 (MQ=255)

                                                             cccGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGAc                                                                                            >  3:695290/1‑150 (MQ=255)

                                                              ccGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAg                                                                                          <  1:22742/151‑1 (MQ=255)

                                                              ccGGCAATGGGAAATCAACTGCTGAACCGGCGGTTGTAGTTCCTTTAATAATTTCTCCTCTGCGGCAGGGTTATCAACCATTGCATCCTAGTAGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAg                                                                                          <  7:370975/151‑1 (MQ=255)

                                                               cGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAgg                                                                                         <  3:41046/151‑1 (MQ=255)

                                                                 gCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCa                                                                                       <  4:1519166/151‑1 (MQ=255)

                                                                  cAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCa                                                                                       >  8:452937/1‑150 (MQ=255)

                                                                   aaTGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCaaa                                                                                     <  7:1852418/151‑1 (MQ=255)

                                                                        gAAATCACCTGCTGAAGCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCa                                                                                <  6:1341628/151‑1 (MQ=255)

                                                                        gAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATGAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAAcc                                                                                 >  3:1242784/1‑150 (MQ=255)

                                                                          aaTCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCAtc                                                                              <  4:574382/151‑1 (MQ=255)

                                                                          aaTCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCAtc                                                                              >  5:1282638/1‑151 (MQ=255)

                                                                          aaTCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCAtc                                                                              <  4:508339/151‑1 (MQ=255)

                                                                           aTCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCAtct                                                                             >  2:864938/1‑151 (MQ=255)

                                                                             cACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTcg                                                                           <  6:1682705/151‑1 (MQ=255)

                                                                             cACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTcg                                                                           >  7:930322/1‑151 (MQ=255)

                                                                             cACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTcg                                                                           <  7:1977356/151‑1 (MQ=255)

                                                                              aCCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTcgc                                                                          >  5:851678/1‑151 (MQ=255)

                                                                              aCCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTAGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTcgc                                                                          <  7:1312723/151‑1 (MQ=255)

                                                                                  gctgAACCGGCGGTTTTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCa                                                                       <  7:237658/150‑1 (MQ=255)

                                                                                  gctgAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCaa                                                                      <  6:1465370/151‑1 (MQ=255)

                                                                                  gctgAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCa                                                                       <  3:1932843/150‑1 (MQ=255)

                                                                                    tgAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGc                                                                    >  6:132155/1‑151 (MQ=255)

                                                                                    tgAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGc                                                                    >  8:647341/1‑151 (MQ=255)

                                                                                    ggAAGCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGc                                                                    <  2:60726/150‑1 (MQ=255)

                                                                                     gAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAg                                                                     <  6:605799/149‑1 (MQ=255)

                                                                                       aCCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGAt                                                                 <  2:1412203/151‑1 (MQ=255)

                                                                                       aCCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGAt                                                                 >  8:1383510/1‑151 (MQ=255)

                                                                                       aCCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGAt                                                                 <  4:1367086/151‑1 (MQ=255)

                                                                                            cggTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAAGCATTGCATGCCAGTCTCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGCATAGAGACAGGCAAAAACGATCTCGCGCAAGCGATTCAGa                                                            <  4:1300549/151‑1 (MQ=255)

                                                                                             ggTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTc                                                               >  6:195539/1‑147 (MQ=255)

                                                                                             ggTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTc                                                               >  2:1751346/1‑147 (MQ=255)

                                                                                             ggTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTc                                                               >  7:571565/1‑147 (MQ=255)

                                                                                              gTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTc                                                               >  7:1015400/1‑146 (MQ=255)

                                                                                              gTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGAATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATc                                                          <  7:851139/151‑1 (MQ=255)

                                                                                                     ttCCTGTAATAATTTCGCCGCTGCGGCATGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCCCGCAAGCGATTCAGATCTTTAACt                                                   >  2:1894281/1‑151 (MQ=255)

                                                                                                         tGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGtt                                               >  5:956850/1‑151 (MQ=255)

                                                                                                               aatTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGc                                          <  3:842774/150‑1 (MQ=255)

                                                                                                               aatTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGc                                          <  2:924136/150‑1 (MQ=255)

                                                                                                                  ttCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCACAAGCGATTCAGATCTTTAACTGGTTTCAGCaaa                                       <  8:1214696/150‑1 (MQ=255)

                                                                                                                    cggagcTGCGGCACGGTTATCAACCATTTCATCCGAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGGATGCGATTCAGATCTTTAACTGGTTTCAGGAAAAc                                     <  4:20698/146‑1 (MQ=255)

                                                                                                                     gccgcTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACAt                                   >  7:295012/1‑151 (MQ=255)

                                                                                                                      ccgcTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACa                                    <  3:1769027/149‑1 (MQ=255)

                                                                                                                       cgcTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCt                                 <  7:1225824/151‑1 (MQ=255)

                                                                                                                       cgcTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCt                                 <  3:1486476/151‑1 (MQ=255)

                                                                                                                        gcTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCtt                                >  8:584010/1‑151 (MQ=255)

                                                                                                                        gcTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCtt                                >  5:733973/1‑151 (MQ=255)

                                                                                                                        gcTGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCtt                                >  8:147564/1‑151 (MQ=255)

                                                                                                                          tGCGGCAGGGTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGAGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCTTc                               >  1:324630/1‑150 (MQ=255)

                                                                                                                          cgccgCACGGTTATCAACCAATGCATCCTACTCGCGAAAAACCCTTTCCTCCTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCTTCa                              <  2:1042608/147‑1 (MQ=255)

                                                                                                                                  ggTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCTTCAACGCCCAg                      <  8:208176/151‑1 (MQ=255)

                                                                                                                                   gTTATCAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCTTCAACGccc                        >  8:1590646/1‑148 (MQ=255)

                                                                                                                                        cAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCTTCAACGCCCAGACGTaa                <  3:1472132/151‑1 (MQ=255)

                                                                                                                                        cAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCTTCAACGCCCAGACGTaa                <  3:594084/151‑1 (MQ=255)

                                                                                                                                        cAACCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCTTCAACGCCCAGACGTaa                <  6:739892/151‑1 (MQ=255)

                                                                                                                                         aaCCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCTTCAACGCCCAGACGTAAc               <  6:315449/151‑1 (MQ=255)

                                                                                                                                          aCCATTGCATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCTTCAACGCCCAGACGTAACg              <  5:184039/151‑1 (MQ=255)

                                                                                                                                              ttGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCTTCAACGCCCAGCCGTAACGCttt          >  8:1019517/1‑151 (MQ=255)

                                                                                                                                                 cATCCCAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCTTCAACGCCCAGACGTAACGCTTTGGc       >  4:1790062/1‑151 (MQ=255)

                                                                                                                                                  aTCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCTTCAACGCCCAGACGTAACGCTTTGGCa      <  7:774191/151‑1 (MQ=255)

                                                                                                                                                     cTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCTTCAACGCCCAGACGTAACGCTTTGGCAat    >  8:291248/1‑150 (MQ=255)

                                                                                                                                                     cTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCTTCAACGCCCAGACGTAACGCTTTGGCAat    >  1:1154904/1‑150 (MQ=255)

AGTGCGGCAATATCAAGCACCAGGCCGGGTTTGTCCGCGGCAACCAATTGACGATAATTAACCCGGCAATGGGAAATCACCTGCTGAACCGGCGGTTGTAGTTCCTGTAATAATTTCGCCGCTGCGGCAGGGTTATCAACCATTGCATCCTAGTCGCGAAAAAGCCTTTCCTCTTCCTCAACGCGCGAATTAAACATGCTGGGATAGAGACAGGCAAAAACCATCTCGCGCAAGCGATTCAGATCTTTAACTGGTTTCAGCAAAACATCTTCAACGCCCAGACGTAACGCTTTGGCAATAT  >  DH1/2587659‑2587959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`