BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	2,732,543	0	A	G	57.3%	244.1 / inf	452	S135P (TCG→CCG)	yceD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (94/99); new base G (125/134); total (219/233)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 461 total aligned reads displayed.

GGTTTATCCCCGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACGTTGTGT  >  DH1/2732393‑2732692

   ttATCCCCGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGaaa                                                                                                                                                     <  2:1530099/150‑1 (MQ=255)

   ttATCCCCGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAAc                                                                                                                                                    >  5:1291300/1‑151 (MQ=255)

   ttATCCCCGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTAAGAATAATGCACCGgaa                                                                                                                                                     >  8:582484/1‑150 (MQ=255)

     aTCCCCGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACt                                                                                                                                                   >  5:1837402/1‑150 (MQ=255)

        cccGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCg                                                                                                                                               >  2:407277/1‑151 (MQ=255)

        cccGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCg                                                                                                                                               <  4:312530/151‑1 (MQ=255)

          cGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGc                                                                                                                                             >  8:1171322/1‑151 (MQ=255)

            aTCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCaa                                                                                                                                           <  5:517628/151‑1 (MQ=255)

              ccAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAgg                                                                                                                                         >  3:408916/1‑151 (MQ=255)

              ccAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCGTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAg                                                                                                                                          <  3:996687/150‑1 (MQ=255)

               cAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGc                                                                                                                                        <  6:566167/151‑1 (MQ=255)

                aaCGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCg                                                                                                                                       <  7:376339/151‑1 (MQ=255)

                aaCGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCg                                                                                                                                       >  6:1557746/1‑151 (MQ=255)

                 aCGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGa                                                                                                                                      <  7:1108793/151‑1 (MQ=255)

                     ggAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGgat                                                                                                                                  <  4:1631271/151‑1 (MQ=255)

                      gAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGgatg                                                                                                                                 >  3:1535510/1‑151 (MQ=255)

                       aGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGgatg                                                                                                                                 >  1:1377672/1‑150 (MQ=255)

                         cACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGAtt                                                                                                                              >  5:658420/1‑151 (MQ=255)

                          aCCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGAttt                                                                                                                             >  7:696724/1‑151 (MQ=255)

                             aaTTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCAt                                                                                                                          <  8:688811/151‑1 (MQ=255)

                                tacttacGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCtt                                                                                                                       >  8:1258830/1‑151 (MQ=255)

                                 acttacGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTAACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTc                                                                                                                      >  4:1787425/1‑151 (MQ=255)

                                  cttacGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTc                                                                                                                      >  1:1544767/1‑150 (MQ=255)

                                       gCTTTAAGCTGGCTAATACGGCAAATGTGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTTTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCAt                                                                                                                <  3:1779740/151‑1 (MQ=255)

                                        cTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACAAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCAtt                                                                                                               >  2:811386/1‑151 (MQ=255)

                                         tttAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTg                                                                                                              >  2:635143/1‑151 (MQ=255)

                                          ttAAGCTGGCTAATACGTCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTg                                                                                                              <  7:1896741/150‑1 (MQ=255)

                                          ttAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCAtt                                                                                                               <  1:391636/149‑1 (MQ=255)

                                          ttAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCAtt                                                                                                               <  6:1228339/149‑1 (MQ=255)

                                          ttAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTg                                                                                                              <  4:1516060/150‑1 (MQ=255)

                                          ttAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCAtt                                                                                                               <  6:855105/149‑1 (MQ=255)

                                          ttAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCAtt                                                                                                               <  7:163052/149‑1 (MQ=255)

                                           tAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGc                                                                                                             <  1:991590/150‑1 (MQ=255)

                                                 ggCTAATACGGGAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGa                                                                                                      <  1:479524/151‑1 (MQ=255)

                                                 ggCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCa                                                                                                        <  1:1468510/149‑1 (MQ=255)

                                                   cTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAAGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGc                                                                                                         >  2:952075/1‑146 (MQ=255)

                                                    tAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGc                                                                                                         >  3:1484855/1‑145 (MQ=255)

                                                     aaTACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATc                                                                                                    <  3:696467/149‑1 (MQ=255)

                                                     aaTACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCg                                                                                                   <  2:1234357/150‑1 (MQ=255)

                                                     aaTACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCg                                                                                                   <  8:1038623/150‑1 (MQ=255)

                                                       tACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTAACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGAtt                                                                                                >  6:1386069/1‑151 (MQ=255)

                                                             aaaTGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCg                                                                                          >  3:1856764/1‑151 (MQ=255)

                                                             aaaTGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCg                                                                                          >  7:1916859/1‑151 (MQ=255)

                                                              aaTGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCg                                                                                          >  8:202817/1‑150 (MQ=255)

                                                              aaTGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCg                                                                                          >  7:883773/1‑150 (MQ=255)

                                                                  ggTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTc                                                                                     <  6:1621159/151‑1 (MQ=255)

                                                                     ttGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCAtt                                                                                                               >  5:1002384/1‑122 (MQ=255)

                                                                       ggCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTaa                                                                                <  1:10565/151‑1 (MQ=255)

                                                                       ggCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTa                                                                                 <  8:375248/150‑1 (MQ=255)

                                                                       ggCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTa                                                                                 <  4:1164248/150‑1 (MQ=255)

                                                                        gCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAAc                                                                               <  2:1323380/151‑1 (MQ=255)

                                                                        gCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAAc                                                                               >  4:1388913/1‑151 (MQ=255)

                                                                        gCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAAc                                                                               >  5:954465/1‑151 (MQ=255)

                                                                          ttttGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCt                                                                             >  2:200091/1‑151 (MQ=255)

                                                                           tttGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAAcc                                                                              <  3:196766/149‑1 (MQ=255)

                                                                             tGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACTCTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCa                                                                           <  5:1764381/150‑1 (MQ=255)

                                                                               cgcTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATc                                                                        <  3:1207595/151‑1 (MQ=255)

                                                                                   tcttcAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGtt                                                                    >  8:924232/1‑151 (MQ=255)

                                                                                   tcttcAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGt                                                                     <  4:502127/150‑1 (MQ=255)

                                                                                      tcAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGtt                                                                    <  4:849377/148‑1 (MQ=255)

                                                                                      tcAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCa                                                                  <  5:622794/150‑1 (MQ=255)

                                                                                       cAGGCAGTTCACCAAAGACGATGTGCGCTTCGGACACTTCAGAGTGTTCAGAATCATGCACCGAAACTACCGGCTAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATa                                                                <  8:1474035/151‑1 (MQ=255)

                                                                                       cAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCAt                                                                 >  7:270146/1‑150 (MQ=255)

                                                                                        aGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATAc                                                               >  1:77587/1‑151 (MQ=255)

                                                                                         ggCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCCAATTCGTTAACCTCAATCGGTTCATACg                                                              <  6:1851981/151‑1 (MQ=255)

                                                                                          gCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGc                                                             <  5:1478246/151‑1 (MQ=255)

                                                                                           cAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCATGCAGATCGATTTCACAGATTTCGTTAACCTCAATCGGTTCATACGCt                                                            >  6:229803/1‑151 (MQ=255)

                                                                                               tccccAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCg                                                        >  7:1964068/4‑151 (MQ=255)

                                                                                                  ccAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAACCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCa                                                     <  8:1844611/151‑1 (MQ=255)

                                                                                                    aaaTACGATGTCCGATTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCTGCAAGGCGAGGATGATTTGATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGt                                                   <  3:34647/151‑1 (MQ=255)

                                                                                                    aaaGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGt                                                   >  6:396297/1‑151 (MQ=255)

                                                                                                      aGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGc                                                 >  2:1807179/1‑151 (MQ=255)

                                                                                                         ccATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTc                                              >  3:1706083/1‑151 (MQ=255)

                                                                                                          cATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTc                                              >  6:657411/1‑150 (MQ=255)

                                                                                                           aTGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCAGGCAGTGCTTCac                                            >  7:1268038/1‑150 (MQ=255)

                                                                                                                 gCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGtt                                      >  7:1840963/1‑151 (MQ=255)

                                                                                                                  cTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTc                                     >  8:140788/1‑151 (MQ=255)

                                                                                                                     cGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGt                                   <  3:1108228/150‑1 (MQ=255)

                                                                                                                       gACACTTCACAGTGTTCAGAATGATGCTCCGGAACTAGCTGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTg                                <  1:885167/151‑1 (MQ=255)

                                                                                                                         cacTTCACAGTGTTCAGAATCATGCACAGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGAAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGa                               >  3:1394561/1‑150 (MQ=255)

                                                                                                                          acTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAAc                             >  1:23530/1‑151 (MQ=255)

                                                                                                                          acTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAAc                             >  2:1883098/1‑151 (MQ=255)

                                                                                                                             tCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACg                            <  8:783625/149‑1 (MQ=255)

                                                                                                                             tCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGc                           >  4:1721362/1‑150 (MQ=255)

                                                                                                                               ccaGTGTTCAGATTCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACGGATTTCGTTAACCTCAATGTCTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGcaca                        <  4:589547/150‑1 (MQ=255)

                                                                                                                                 aGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAgg                      <  1:1218188/151‑1 (MQ=255)

                                                                                                                                    gtTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACt                   <  3:792673/151‑1 (MQ=255)

                                                                                                                                    gtTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACt                   <  7:1218801/151‑1 (MQ=255)

                                                                                                                                    gtTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACt                   >  4:1892103/1‑151 (MQ=255)

                                                                                                                                      tCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTACACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTa                  >  2:753855/1‑150 (MQ=255)

                                                                                                                                       cAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTaaa                <  4:1850968/151‑1 (MQ=255)

                                                                                                                                       cAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTaa                 <  7:583151/150‑1 (MQ=255)

                                                                                                                                        aGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATAAATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGATTCAGCCTGTTCGTCTGAACGCACAGGACTaaa                >  2:1105292/1‑150 (MQ=255)

                                                                                                                                             cATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTACGTCTGAACGCACAGGACTAAAACAAt           >  8:1601610/1‑150 (MQ=255)

                                                                                                                                               tGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACg        >  8:1927225/1‑151 (MQ=255)

                                                                                                                                                gCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACg        <  7:680827/150‑1 (MQ=255)

                                                                                                                                                gCACCGAAACTAACGGCAAGGCGAGGATTATTTCATCTTCAACCATTGCAATCAGATCGATTTCACGGAATTCGTTAACCTCATTCGGTTCATACGCTTCCGGCAGTGCTTGAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACg        <  4:760222/150‑1 (MQ=255)

                                                                                                                                                 cACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACGtt      >  6:1698010/1‑151 (MQ=255)

                                                                                                                                                    cGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACGTtgtg   >  6:1113249/1‑151 (MQ=255)

                                                                                                                                                      aaaCTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACGTTgtgt  >  7:1011359/1‑150 (MQ=255)

GGTTTATCCCCGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACGTTGTGT  >  DH1/2732393‑2732692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`