Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1298,2460TC55.2% 186.8 / inf 543V436A (GTC→GCC) ggtgamma‑glutamyltransferase
Reads supporting (aligned to +/- strand):  ref base T (125/118);  new base C (145/154);  total (270/273)
Fisher's exact test for biased strand distribution p-value = 5.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-01

Only 100 of 544 total aligned reads displayed.

GAGAGTAATCAAACTACCCATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACCTGGCTGGTTACCGGTAGCCCAGGCGGTAGCCG  >  DH1/298098‑298396
                                                                                                                                                    |                                                                                                                                                      
gagaGTAATCAAACTACCCATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCa                                                                                                                                                      <  8:143090/151‑1 (MQ=255)
 agagTAATCAAACTACCCATTACTCAGTGGGGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCaa                                                                                                                                                     <  4:1917956/151‑1 (MQ=255)
  gagTAATCAAACTACCCATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCaaa                                                                                                                                                    <  5:511961/151‑1 (MQ=255)
    gTAATCAAACTACCCATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAAcc                                                                                                                                                  <  1:1595488/151‑1 (MQ=255)
      aaTCAAACTACCCATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCgg                                                                                                                                                >  8:1790761/1‑151 (MQ=255)
      aaTCAAACTACCCATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCg                                                                                                                                                 <  7:1733111/150‑1 (MQ=255)
       aTCAAACTACCCATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCggg                                                                                                                                               >  8:1380276/1‑151 (MQ=255)
       aTCAAACTACCCATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGCACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCggg                                                                                                                                               >  8:788527/1‑151 (MQ=255)
        tCAAACTACCCATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGc                                                                                                                                              >  6:364759/1‑151 (MQ=255)
         cAAACTACCCATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGc                                                                                                                                              <  6:1543438/150‑1 (MQ=255)
           aaCTACCCATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTa                                                                                                                                           <  4:150233/151‑1 (MQ=255)
            aCTACCCATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTAc                                                                                                                                          <  3:1201952/151‑1 (MQ=255)
             cTACCCATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGCATGATTTCTCCGCCAAACAGGGCGTAcc                                                                                                                                         >  6:616051/1‑151 (MQ=255)
              tACACATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACATTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCAGCCAAACCGGGCGTACCg                                                                                                                                        >  3:129647/1‑151 (MQ=255)
                cccATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGaa                                                                                                                                      <  2:376704/151‑1 (MQ=255)
                 ccATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAAc                                                                                                                                     <  8:154944/151‑1 (MQ=255)
                      aCTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGttt                                                                                                                                 >  8:882329/1‑150 (MQ=255)
                         cAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACgg                                                                                                                             <  3:1850234/151‑1 (MQ=255)
                         cAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTAc                                                                                                                               <  5:195042/149‑1 (MQ=255)
                              gtggATAAAGATGGTAACGCGGGGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGGCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCtgg                                                                                                                        <  4:172858/151‑1 (MQ=255)
                                    aaaGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCg                                                                                                                  >  3:836218/1‑151 (MQ=255)
                                       gATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTg                                                                                                               >  5:65090/1‑151 (MQ=255)
                                        aTGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTg                                                                                                               >  1:602510/1‑150 (MQ=255)
                                        aTGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGa                                                                                                              <  3:178823/151‑1 (MQ=255)
                                          ggTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATg                                                                                                            >  4:1270463/1‑151 (MQ=255)
                                             aaCGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGcc                                                                                                          >  4:702123/1‑150 (MQ=255)
                                             aaCGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGcc                                                                                                          <  8:583185/150‑1 (MQ=255)
                                               cgcgGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAAc                                                                                                       >  5:1261395/1‑151 (MQ=255)
                                               cgcgGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAAc                                                                                                       >  6:1684909/1‑151 (MQ=255)
                                                 cggtggcggtgACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGc                                                                                                     <  3:597685/151‑1 (MQ=255)
                                                   gtggcggtgACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCg                                                                                                   >  1:1255284/1‑151 (MQ=255)
                                                    tggcggtgACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGt                                                                                                  >  4:52348/1‑151 (MQ=255)
                                                     ggcggtgACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTc                                                                                                 <  5:1138971/151‑1 (MQ=255)
                                                      gcggtgACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCg                                                                                                <  1:210969/151‑1 (MQ=255)
                                                      gcggtgACCTATACGCTGAACACCACCTTCGGTACGGGCATTGGCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCg                                                                                                <  3:400301/151‑1 (MQ=255)
                                                        ggtgACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCggg                                                                                              <  8:1841095/151‑1 (MQ=255)
                                                           ggccTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATACCCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGATCGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCg                                                                                           >  1:1761450/3‑151 (MQ=255)
                                                           gACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCTAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCg                                                                                           >  6:198360/1‑151 (MQ=255)
                                                           gACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCg                                                                                           <  2:1834550/151‑1 (MQ=255)
                                                            aCCTATACGCTGAACACCTCCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCg                                                                                           >  8:1227871/1‑150 (MQ=255)
                                                            aCCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCg                                                                                           >  4:791993/1‑150 (MQ=255)
                                                             ccTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGa                                                                                          >  7:1145333/1‑150 (MQ=255)
                                                                ataCGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACaa                                                                                      <  5:427194/151‑1 (MQ=255)
                                                                  aCGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAAc                                                                                    <  7:1608182/151‑1 (MQ=255)
                                                                       gAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCg                                                                               >  7:859437/1‑151 (MQ=255)
                                                                         acacCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCt                                                                             >  8:394955/1‑151 (MQ=255)
                                                                          caccacCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTg                                                                            <  7:384349/151‑1 (MQ=255)
                                                                           accaccTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTgt                                                                           <  1:462975/151‑1 (MQ=255)
                                                                               ccTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTgtcgtc                                                                       <  3:1125323/151‑1 (MQ=255)
                                                                                cTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGtcgtcg                                                                      >  6:74435/1‑151 (MQ=255)
                                                                                cTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGtcgtcg                                                                      >  1:332800/1‑151 (MQ=255)
                                                                                 ttCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGtcgtcg                                                                      >  2:572685/1‑150 (MQ=255)
                                                                                 ttCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGtcgtcg                                                                      >  2:1420392/1‑150 (MQ=255)
                                                                                  tCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGTGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGtcgtcg                                                                      <  8:1673498/149‑1 (MQ=255)
                                                                                   cGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATg                                                                   <  7:1516001/151‑1 (MQ=255)
                                                                                     gTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTc                                                                 <  2:1165246/151‑1 (MQ=255)
                                                                                     gTACGGGCATTGTCGCGGGCGAGAGCGGTATTCAGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTc                                                                 <  7:1023228/151‑1 (MQ=255)
                                                                                       aCGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGc                                                               >  7:700649/1‑151 (MQ=255)
                                                                                       aCGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGc                                                               >  5:820257/1‑151 (MQ=255)
                                                                                         gggCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCg                                                             <  1:869737/151‑1 (MQ=255)
                                                                                             aTTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGAcc                                                          >  6:1247036/1‑150 (MQ=255)
                                                                                             aTTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCa                                                         <  5:1482486/151‑1 (MQ=255)
                                                                                             aTTGTCGCGGGAGAGAGAGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGAGTACCGAACGTTTACGGGCGGCTGGGAGTTGATGCCAACGCCGTCTGTCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCa                                                         <  2:199254/151‑1 (MQ=255)
                                                                                                  cgcgGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATtgt                                                     <  5:1647603/150‑1 (MQ=255)
                                                                                                    cgGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTgtggt                                                  <  6:1600645/151‑1 (MQ=255)
                                                                                                     gggCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTGCGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTgtggtg                                                 >  7:1918959/1‑151 (MQ=255)
                                                                                                     gggCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTgtggtg                                                 >  7:528105/1‑151 (MQ=255)
                                                                                                     gggCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTgtggtg                                                 <  4:1118727/151‑1 (MQ=255)
                                                                                                      ggCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGa                                                <  4:927124/151‑1 (MQ=255)
                                                                                                         gagaGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGaa                                               >  8:1084756/1‑149 (MQ=255)
                                                                                                          agagCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGaa                                               >  5:920198/1‑148 (MQ=255)
                                                                                                          agagCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGaa                                               >  4:1319758/1‑148 (MQ=255)
                                                                                                            agCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACg                                          <  2:1597951/151‑1 (MQ=255)
                                                                                                             gCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACgg                                         <  5:1877958/151‑1 (MQ=255)
                                                                                                              cGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCTAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGt                                        >  6:509900/1‑151 (MQ=255)
                                                                                                                 tATTCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTaa                                      >  6:1799278/1‑150 (MQ=255)
                                                                                                                  aTTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTaaa                                     <  1:93752/150‑1 (MQ=255)
                                                                                                                    tCTGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAAcc                                  >  6:109541/1‑151 (MQ=255)
                                                                                                                      tGCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACctg                                >  7:475467/1‑151 (MQ=255)
                                                                                                                       gCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACctgg                               >  6:1361658/1‑151 (MQ=255)
                                                                                                                       gCTTAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACct                                 <  3:1941610/149‑1 (MQ=255)
                                                                                                                       gCTTAATAACCAGATGGAGTATTTCTCCGTCAAACGGGGCGTACTGAAGTTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGGCGCCGACCATTGTGGTGAAAGACGGTAAAACctgg                               <  2:1879904/151‑1 (MQ=255)
                                                                                                                          taataaCCAGTTGGATGATTTCTCCGCCAAACGGGGCGTACCGAACGTTTTCGGGATGGTGGTCTGTGATGCCAACGCCGGCGGGCCGTACAAACGCCCGCTGTCGTCGATGTCGGCGACCATTGTGGTGAAAGACGGTAAAACctggct                             <  7:955764/150‑1 (MQ=255)
                                                                                                                          taataaCCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTCGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACctggctg                            >  3:1565363/1‑151 (MQ=255)
                                                                                                                          taataaCCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACctggctg                            >  2:817885/1‑151 (MQ=255)
                                                                                                                           aataaCCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGACCGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACctggctg                            <  4:412862/150‑1 (MQ=255)
                                                                                                                            ataaCCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATAGTGGTGAAAGACGGTAAAACCTGGCTGGt                          >  3:1065086/1‑151 (MQ=255)
                                                                                                                                ccAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGCAAGACGGTAAAACCTGGCTGGTTAcc                      >  6:904290/1‑151 (MQ=255)
                                                                                                                                ccAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACCTGGCTGGTTAcc                      >  3:1683576/1‑151 (MQ=255)
                                                                                                                                 cAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACCTGGCTGGTTACCg                     <  8:1959974/151‑1 (MQ=255)
                                                                                                                                  aGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACCTGGCTGGTTACCgg                    >  3:99018/1‑151 (MQ=255)
                                                                                                                                   gatggatgATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACCTGGCTGGTTACCGGt                   <  1:713472/151‑1 (MQ=255)
                                                                                                                                    atggatgATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACCTGGCTGGTTACCGGTa                  <  3:651818/151‑1 (MQ=255)
                                                                                                                                    atggatgATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACCTGGCTGGTTACCGGTa                  <  1:1359935/151‑1 (MQ=255)
                                                                                                                                       gatgatTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCATCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACCTGGCTGGTTACCGGTAGcc               <  7:1822850/151‑1 (MQ=255)
                                                                                                                                         tgatTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACCTGGCTGGTTACCGGTAGccc              >  1:107271/1‑150 (MQ=255)
                                                                                                                                         ggatTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCCACGCCGTCGGGCCGAACAACCGCCCGCTGTCGTCGATGTCGCCGACCATTGGGGTGAAAGACGGTAAAACCTGGCTGGTTACCGGTAGCCCa             <  7:1316764/150‑1 (MQ=255)
                                                                                                                                           atTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACCTGGCTGGTTACCGGTAGCCCAgg           >  3:1442975/1‑151 (MQ=255)
                                                                                                                                               ctcCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACCTGGCTGGTTACCGGTAGCCCAGGCGGt       >  7:1363150/1‑151 (MQ=255)
                                                                                                                                                   gTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACCTGGCTGGTTACCGGTAGCCCAGGCGGTAGcc   >  5:264412/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
GAGAGTAATCAAACTACCCATTACTCAGTGGTGGATAAAGATGGTAACGCGGTGGCGGTGACCTATACGCTGAACACCACCTTCGGTACGGGCATTGTCGCGGGCGAGAGCGGTATTCTGCTTAATAACCAGATGGATGATTTCTCCGTCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGTGAAAGACGGTAAAACCTGGCTGGTTACCGGTAGCCCAGGCGGTAGCCG  >  DH1/298098‑298396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: