Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,831,5490AC54.2% 275.0 / inf 415V523V (GTT→GTGyeeJinverse autotransporter adhesin‑like protein YeeJ
Reads supporting (aligned to +/- strand):  ref base A (96/93);  new base C (113/111);  total (210/205)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

Only 100 of 421 total aligned reads displayed.

GTCGAGAGCACCAGCCCGACAACAGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCTTTACCCGTTGTGACCACTTTGCCA  >  DH1/1831400‑1831699
                                                                                                                                                     |                                                                                                                                                      
gTCGAGAGCACCAGCCCGACAACAGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACa                                                                                                                                                        >  8:2097/1‑150 (MQ=255)
   gagaGCACCAGCCCGACAACAGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGaccacc                                                                                                                                                     >  1:1226915/1‑150 (MQ=255)
   gagaGCACCAGCCCGACAACAGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAAcc                                                                                                                                                     >  2:1453945/1‑150 (MQ=255)
    agagCACCAGCCCGACAACAGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCAt                                                                                                                                                   <  5:405095/151‑1 (MQ=255)
     gagCACCAGCCCGACAACAGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATg                                                                                                                                                  <  5:550699/151‑1 (MQ=255)
          ccAGCCCGACAACAGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTg                                                                                                                                             <  3:691616/151‑1 (MQ=255)
            aGCCCGACAACAGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGtt                                                                                                                                           <  5:910607/151‑1 (MQ=255)
              cccGACAAGAGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCGGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCa                                                                                                                                         <  1:1115481/151‑1 (MQ=255)
              cccGACAACAGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTc                                                                                                                                          >  8:654608/1‑150 (MQ=255)
               ccGACAACAGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCAc                                                                                                                                        >  4:255887/1‑151 (MQ=255)
                cGACAACAGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACg                                                                                                                                       >  3:1328995/1‑151 (MQ=255)
                 gACAACAGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACg                                                                                                                                       >  3:327677/1‑150 (MQ=255)
                 gACAACAGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGa                                                                                                                                      <  5:1499119/151‑1 (MQ=255)
                   caacaGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGAtt                                                                                                                                    <  2:916887/151‑1 (MQ=255)
                       aGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGAc                                                                                                                                >  3:1615112/1‑151 (MQ=255)
                         gATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACaa                                                                                                                              <  4:1547842/151‑1 (MQ=255)
                           ttACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAAt                                                                                                                            >  3:1482705/1‑151 (MQ=255)
                             aCCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAAtt                                                                                                                           <  1:1336787/150‑1 (MQ=255)
                             aCCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTg                                                                                                                          <  2:1551751/151‑1 (MQ=255)
                             aCCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAAtt                                                                                                                           <  3:1828559/150‑1 (MQ=255)
                                    gcATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGACCGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTg                                                                                                                    >  6:368039/1‑150 (MQ=255)
                                    gcATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTg                                                                                                                    <  6:787375/150‑1 (MQ=255)
                                     catcatGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGAc                                                                                                                  >  7:1548243/1‑151 (MQ=255)
                                       tcatGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACAt                                                                                                                >  3:1243632/1‑151 (MQ=255)
                                        catGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACAt                                                                                                                <  5:1475174/150‑1 (MQ=255)
                                        catGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATc                                                                                                               <  5:243056/151‑1 (MQ=255)
                                        catGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATc                                                                                                               <  5:880460/151‑1 (MQ=255)
                                           gagcAATAAAAGGCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATTTTTCGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAAAGACCACCATGCTCTGTGCACGAGTCGACAAATTGCCTTTGACATCTTc                                                                                                            <  1:28247/149‑1 (MQ=255)
                                            cgcAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTcg                                                                                                           <  8:395205/151‑1 (MQ=255)
                                             gcAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTcgg                                                                                                          >  3:350325/1‑151 (MQ=255)
                                             gcAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTcgg                                                                                                          >  3:958302/1‑151 (MQ=255)
                                               aaTAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTAGCTTAGCGTAGGTGCCTGAACGCCCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTcggcg                                                                                                        >  2:165526/1‑151 (MQ=255)
                                                aTAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTcggcgg                                                                                                       >  3:1949772/1‑151 (MQ=255)
                                                  aaaaGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGt                                                                                                      <  5:110351/150‑1 (MQ=255)
                                                  aaaaGTCAGTCTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTg                                                                                                     >  7:665042/1‑151 (MQ=255)
                                                    aaGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGAc                                                                                                   >  2:1943432/1‑151 (MQ=255)
                                                        cAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTc                                                                                                <  1:1733399/150‑1 (MQ=255)
                                                        cAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATCCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTc                                                                                                >  2:1309398/1‑150 (MQ=255)
                                                          gtgtGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAAt                                                                                             >  6:1784833/1‑151 (MQ=255)
                                                           tgtgGCGGTTGAATCGGAATCCGCGTTCAATGTTTGGGAACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTCTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGAGACTTCAATc                                                                                            >  6:973636/1‑151 (MQ=255)
                                                            gtgGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATc                                                                                            <  1:864666/150‑1 (MQ=255)
                                                            gtgGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTATCTGGCTTAGCGTAGGTCCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTATGACATCTTCGGCGGTGACTACAATCg                                                                                           >  4:663238/1‑151 (MQ=255)
                                                              ggCGGTTGACTGGGAATCCGAGTTGAATGTTTGGGTACTTAACGATACCGATGAATCTTTCTGGCTTAGCGTATGTGCCTGAACGACTACCATTCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGc                                                                                         <  5:747885/151‑1 (MQ=255)
                                                              ggCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCgg                                                                                          <  6:1706484/150‑1 (MQ=255)
                                                               gCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTATCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTATGCGGTGACTTCAATCGGcc                                                                                        <  6:687229/151‑1 (MQ=255)
                                                                 ggTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAg                                                                                      <  1:404232/151‑1 (MQ=255)
                                                                   ttGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGgt                                                                                    >  4:977607/1‑151 (MQ=255)
                                                                    tGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGgtg                                                                                   >  8:1291426/1‑151 (MQ=255)
                                                                    tGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGgt                                                                                    <  3:541412/150‑1 (MQ=255)
                                                                    tGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAAGCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGgt                                                                                    <  1:196653/150‑1 (MQ=255)
                                                                       aTGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGtt                                                                                 >  8:918762/1‑150 (MQ=255)
                                                                       aTGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGtt                                                                                 >  4:561818/1‑150 (MQ=255)
                                                                          ggAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCg                                                                             <  6:527747/151‑1 (MQ=255)
                                                                             aTCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGtt                                                                          >  2:550574/1‑151 (MQ=255)
                                                                                cgcgTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCt                                                                       >  7:213113/1‑151 (MQ=255)
                                                                                 gcgTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCCTGGTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTg                                                                      >  4:802852/1‑151 (MQ=255)
                                                                                 gcgTTCAATGGTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGGGACTTCAATCGGCCAGGTGTTATCGGTTTCTg                                                                      <  1:1021566/151‑1 (MQ=255)
                                                                                  cgTTCAATGTTTGCGTACTTAACGATACCGAGGAATCTTTGTGGCTTTGCGTAGGGGCCTGAAGGACAACCATGCTCTGTGCACGATTCGACAAAATGCCTTTGACATCTTGGGGGTTGACTTGTATCGGCCTGGTGTTATCGGTTTCTgg                                                                     <  8:1527608/151‑1 (MQ=255)
                                                                                   gTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGc                                                                    >  1:526218/1‑151 (MQ=255)
                                                                                     tCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCg                                                                   <  8:84274/150‑1 (MQ=255)
                                                                                      cAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGt                                                                  >  6:1418525/1‑150 (MQ=255)
                                                                                          gTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTg                                                              <  1:1381559/150‑1 (MQ=255)
                                                                                            ttGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTg                                                           >  6:1534039/1‑151 (MQ=255)
                                                                                             tGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTg                                                           <  7:888969/150‑1 (MQ=255)
                                                                                             tGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTg                                                           <  8:1700996/150‑1 (MQ=255)
                                                                                              gggTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGa                                                          >  1:97345/1‑150 (MQ=255)
                                                                                              gggTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGa                                                          >  3:1087939/1‑150 (MQ=255)
                                                                                               ggTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAAc                                                        <  7:380811/151‑1 (MQ=255)
                                                                                                gTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAAcc                                                       <  6:1255894/151‑1 (MQ=255)
                                                                                                gTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAAcc                                                       <  3:835292/151‑1 (MQ=255)
                                                                                                 tACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGGCTTTGACATCTTCGGCGGTGACTTCAATCGGCCACGTGTTATCTGTTTCTCGCGTACTGGTGAACCg                                                      >  8:1658302/1‑151 (MQ=255)
                                                                                                        cGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGTCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGcc                                               >  4:1321742/1‑151 (MQ=255)
                                                                                                         gATACCGAGGAATCTTTGTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCg                                              <  6:19362/151‑1 (MQ=255)
                                                                                                            aCCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGc                                            >  1:839017/1‑150 (MQ=255)
                                                                                                             ccGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAg                                          <  2:1140042/151‑1 (MQ=255)
                                                                                                                 ggAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGt                                       >  5:1234138/1‑150 (MQ=255)
                                                                                                                 ggAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGt                                       >  8:726174/1‑150 (MQ=255)
                                                                                                                  gAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTa                                      >  4:934927/1‑150 (MQ=255)
                                                                                                                   aaTCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAAc                                    <  1:1494804/151‑1 (MQ=255)
                                                                                                                          cTGGCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAat                              >  1:62696/1‑150 (MQ=255)
                                                                                                                            ggCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAat                              <  1:1597859/148‑1 (MQ=255)
                                                                                                                            ggCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATc                           >  5:1628466/1‑151 (MQ=255)
                                                                                                                             gCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCt                          >  4:1094374/1‑151 (MQ=255)
                                                                                                                             gCTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCt                          <  8:1464998/151‑1 (MQ=255)
                                                                                                                              cTTAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCtt                         >  8:1337260/1‑151 (MQ=255)
                                                                                                                               ttAGCGTAGGTGCCTGAACGAGAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATAttt                        >  4:1668062/1‑151 (MQ=255)
                                                                                                                                tAGCGTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCttt                        >  8:449949/1‑150 (MQ=255)
                                                                                                                                    gTAGGTGCCTGAACGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCTTTAccct                   >  7:1245404/1‑150 (MQ=255)
                                                                                                                                    gTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCTTTACCCg                   >  4:1269146/1‑151 (MQ=255)
                                                                                                                                      aGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCTTTACCCGt                  <  4:1876780/150‑1 (MQ=255)
                                                                                                                                       ggTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCTTTACCCGTtg                <  7:1794805/151‑1 (MQ=255)
                                                                                                                                            cTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCTTTACCCGTTGTGAcc           >  5:1950703/1‑151 (MQ=255)
                                                                                                                                              gAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCTTTACCCGTTGTGACCAc         >  2:285969/1‑151 (MQ=255)
                                                                                                                                               aaCGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCTTTACCCGTTGTGACCACt        >  6:1036020/1‑151 (MQ=255)
                                                                                                                                                 cGACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCTTTACCCGTTGTGACCACttt      >  2:402012/1‑151 (MQ=255)
                                                                                                                                                 cGAAAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCTTTACCCGTTGTGACCACttt      <  8:1884992/151‑1 (MQ=255)
                                                                                                                                                  gACCACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCTTTACCCGTTGTGACCACTTTg     >  2:67463/1‑151 (MQ=255)
                                                                                                                                                  gACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCTTTACCCGTTGTGACCACTTTg     <  8:1027263/151‑1 (MQ=255)
                                                                                                                                                   aCAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCACGGTAACCAGAATATCTTTACACGTTGTGACCACTTTGc    >  4:447954/1‑151 (MQ=255)
                                                                                                                                                     aaCCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCTTTACCCGTTGTGACCACTTTGCCa  <  5:180784/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GTCGAGAGCACCAGCCCGACAACAGGATTACCTGCTGCATCATGCGCAATAAAAGTCAGTGTGGCGGTTGAATGGGAATCCGCGTTCAATGTTTGGGTACTTAACGATACCGAGGAATCTTTCTGGCTTAGCGTAGGTGCCTGAACGACAACCATGCTCTGTTCACGATTCGACAAATTGCCTTTGACATCTTCGGCGGTGACTTCAATCGGCCAGGTGTTATCGGTTTCTGGCGTACTGGTGAACCGGTAAGCCGGCAGGGTAACCAGAATATCTTTACCCGTTGTGACCACTTTGCCA  >  DH1/1831400‑1831699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: