Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,184,6490AG57.2% 79.5 / inf 335noncoding (35/75 nt)ECDH1_RS15800tRNA‑TERM
Reads supporting (aligned to +/- strand):  ref base A (81/58);  new base G (132/54);  total (213/122)
Fisher's exact test for biased strand distribution p-value = 1.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 337 total aligned reads displayed.

TTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGTCGGATGCGACGCTGCCGCG  >  DH1/3184499‑3184797
                                                                                                                                                      |                                                                                                                                                    
gcggtgGGGTGTGCCCAACCGGGAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTa                                                                                                                                                      <  1:459758/147‑1 (MQ=37)
             gCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTAGCGCCAAGCGGTAAGGCACAGGATTCTAATTCCGGCATTcc                                                                                                                                         >  3:634316/1‑151 (MQ=255)
              tacaccattaattaaCCTGATTTTGATACAGGCATTCCGAGGTTCGAATCCACGTACTCCAGAAAATTTATTCAAGACGCTTAAATTGTAATTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTcc                                                                                                                                         <  4:1287455/136‑1 (MQ=21)
                     gcaaGGCACCTGATGCTGTTACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGACAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCg                                                                                                                                 <  7:868164/149‑1 (MQ=37)
                           cACCGGATTGTGATACCGGCATTCCGAGCTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTTTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCAtt                                                                                                                                           <  2:1365252/135‑1 (MQ=38)
                           cACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGAAGCTTACCTGGTAAGTGCACCCAGTTGGCGTATCGCCAAGCGCTAAGGCACCGTATTCTACTTCAGTCATTCCGAGTATCGAATCCt                                                                                                                           >  7:1098659/1‑151 (MQ=37)
                                 aTTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTTGGGTATAGCCAAGCTGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGTTTCGAATCCTCGTAcc                                                                                                                     >  7:1459120/1‑151 (MQ=255)
                                      gactcaggCAGTCCGAGGGTCGAATCCTCGTACCCCAGCCAATTTCTTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCa                                                                                                                  <  7:434544/143‑1 (MQ=255)
                                                    gAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCTCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTaaaaaacc                                                                                                  >  5:1525525/1‑149 (MQ=255)
                                                         tCGAATCCTCGTACCCCAGTCAATTTATTAAAGACCCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCt                                                                                             >  2:962358/1‑151 (MQ=255)
                                                         tCGAATCCTCGTACCCCAGCCAATTTATTAAAGAAGCTTACCTTGTAAGTGATCCCAGTGGGGGTTGCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGTATCGACGCCTCGTCCCCCAGCCACATTAAAAAAGCTCGCt                                                                                             >  7:1912416/1‑151 (MQ=25)
                                                                  cGTACCCCAGCAAATTTCTTATATATTCTTAAATTTTAATTGCAACAAGTTGTGGTATAGCAAATCGGTAAGGCACCGGATTCTGATTCCGGCATTGCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGc                                                                                    <  8:311034/151‑1 (MQ=17)
                                                                   gTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCTGCGAGCt                                                                                   >  8:1123375/1‑151 (MQ=255)
                                                                      aagcagacactatataCAAGCCGCTGCCCTTGCAACACCAACCAGGTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCtttt                                                                                <  1:883962/139‑1 (MQ=18)
                                                                           gCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCtt                                                                           >  3:488293/1‑151 (MQ=255)
                                                                              aaTTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGAtttt                                                                         >  7:1019960/1‑150 (MQ=255)
                                                                               aTTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCt                                                                       >  3:601217/1‑151 (MQ=255)
                                                                                tttATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTg                                                                      >  8:1843109/1‑151 (MQ=255)
                                                                                  tATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCg                                                                    >  7:2033689/1‑151 (MQ=255)
                                                                                   aTTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGt                                                                   >  8:742574/1‑151 (MQ=255)
                                                                                        aGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttcatt                                                              >  1:1065005/1‑151 (MQ=255)
                                                                                         gACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttcattc                                                             >  8:1695721/1‑151 (MQ=255)
                                                                                            gctgaaattGGAAGAGCACGCTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAAt                                                          <  7:52517/144‑1 (MQ=21)
                                                                                            gCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCCGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAAt                                                          >  7:1355965/1‑151 (MQ=255)
                                                                                            gCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAAt                                                          >  4:511477/1‑151 (MQ=255)
                                                                                              ttACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGt                                                        >  7:1240552/1‑151 (MQ=255)
                                                                                              ttACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGt                                                        >  7:1847549/1‑151 (MQ=255)
                                                                                               tACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTc                                                       >  3:1184433/1‑151 (MQ=255)
                                                                                               tACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTc                                                       >  8:1189282/1‑151 (MQ=255)
                                                                                               tACCTTGTAAGAGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATg                                                         <  3:870744/149‑1 (MQ=255)
                                                                                                aaaatttAACTGCACTTAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCg                                                      <  4:834250/145‑1 (MQ=255)
                                                                                                aCCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCg                                                      >  8:1914329/1‑151 (MQ=255)
                                                                                                  cTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGa                                                     >  5:1085416/1‑150 (MQ=255)
                                                                                                    tGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGaa                                                    <  5:912002/149‑1 (MQ=255)
                                                                                                     tcaactgcactttgTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATg                                                  <  8:261285/137‑1 (MQ=255)
                                                                                                     tcaaaaccaatttgTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGGTAAGTCGTGATTTGAGCATTTCTGCGTTCATACAATCTCTAATGc                                                 >  5:1766369/14‑151 (MQ=11)
                                                                                                     gTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGc                                                 >  3:301629/1‑151 (MQ=255)
                                                                                                     gTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGc                                                 >  2:249558/1‑151 (MQ=255)
                                                                                                      taaatgcaatttgTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGc                                                 <  4:1896784/138‑1 (MQ=255)
                                                                                                      gaaatgcacgttgTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGc                                                 <  3:1038279/138‑1 (MQ=255)
                                                                                                       aattgcaagttgTTGGGGTATCTCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGa                                               <  2:763937/140‑1 (MQ=21)
                                                                                                       aaaaccaatttgTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAAAAAAGAACCCTACCGCGAGCTTTATGCGATTCTGCGTAAATTCAATCTCGAAGGCg                                                >  1:410797/12‑150 (MQ=11)
                                                                                                        aaaccaatttgTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAAAAAAGCACGCTACCGTGACCTTTATGCATTTCTGCGTTAATTCAATCTCGAATGCGac                                              >  3:200457/11‑150 (MQ=14)
                                                                                                         aagCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATg                                             <  7:696317/149‑1 (MQ=255)
                                                                                                         aaccaatttgTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTATGCGTTTCTGCGTTCATTCAATCTCGAATGCGATg                                             >  4:1110308/10‑151 (MQ=18)
                                                                                                          gccaggttattGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGt                                            <  5:685313/142‑1 (MQ=255)
                                                                                                           gCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTCCCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGtt                                           >  6:92024/1‑151 (MQ=255)
                                                                                                            ccggttattGGGGTATCGCCAAGCGGTAAGGCACCGGATTGTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTACGGCGAGCTTTTTGCTTTTCTGCGttcat                                                               >  2:2014104/8‑130 (MQ=21)
                                                                                                            caatttgTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGttt                                          >  2:484211/7‑150 (MQ=255)
                                                                                                             aactaGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGa                                         <  8:498953/147‑1 (MQ=255)
                                                                                                              cccAGGGGGGGTATCGCCAAGCGGTCAGGCACCTGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGac                                        <  7:74944/151‑1 (MQ=255)
                                                                                                               gttattGGGGTATCGCCAAGCGGTAAGGCACCGGATTATAATTCCGGCATTCCCAGGTTCGAATCCACGTACCCCAGCCAAATTAAAAAAGATCGATACTCCGAGCTTTATGCTTTAATGCGTTCATACAATGACGAATGCCAAGTAGac                                        >  8:1395543/5‑150 (MQ=17)
                                                                                                               ccAGTTGGGGTATCGCCAAGTGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGaca                                       <  3:278741/151‑1 (MQ=255)
                                                                                                               ccAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCTATGTCGAATGCGATGTTGaa                                        >  6:301768/1‑149 (MQ=255)
                                                                                                                 aGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACg                                     >  6:1141655/1‑151 (MQ=255)
                                                                                                                 aGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGacac                                      <  5:785184/150‑1 (MQ=255)
                                                                                                                  tccgctctATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACg                                     <  4:1533777/143‑1 (MQ=255)
                                                                                                                   ttGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTc                                   >  4:316995/1‑151 (MQ=255)
                                                                                                                    tGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCt                                  >  1:263850/1‑151 (MQ=255)
                                                                                                                     ggggTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGCCACGTCtt                                 >  3:1322135/1‑151 (MQ=255)
                                                                                                                      gggTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTa                                <  2:221338/151‑1 (MQ=255)
                                                                                                                      gggTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTa                                <  1:1887076/151‑1 (MQ=255)
                                                                                                                      gggTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAATAAAGCACGCTACGTAGAGCTTTCTCCAAGTGTGCGTTTCTACAATGTCTAATGCGACGTATacacattgat                                >  1:749175/1‑145 (MQ=2)
                                                                                                                       ggTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTAt                               <  1:204368/151‑1 (MQ=255)
                                                                                                                       ggTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTAt                               <  7:603844/151‑1 (MQ=255)
                                                                                                                         tATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAATAAAGCTCCGTACGTGGTGCGTTATGCGATTGTGGGTTGATACAATCTCTAATGCGCGGGTTACACGttgcatcc                             >  3:1688587/1‑144 (MQ=2)
                                                                                                                         tATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAAAAAAGCTCGGTTCGGGGAGCTTTATGCTATTTTGCGTAGAGACAATCTCGAATGCGATGTTGactactataatc                              >  1:1189198/1‑140 (MQ=17)
                                                                                                                          aTCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCt                            <  8:1009529/151‑1 (MQ=255)
                                                                                                                          aTCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCt                            >  1:1544618/1‑151 (MQ=255)
                                                                                                                          aTCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTATGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCt                            >  2:1548242/1‑151 (MQ=255)
                                                                                                                           tCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCtt                           >  1:1120892/1‑151 (MQ=255)
                                                                                                                            cGCCAAGCGGTAAGGCACCGGATTCTGATTGCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTc                          <  4:662618/151‑1 (MQ=255)
                                                                                                                            cGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGGTTCGGGGAGCTTTTTGCGTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACTAGTCTTATCATTc                          >  1:1481482/1‑151 (MQ=25)
                                                                                                                            cGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCtt                           >  3:1766607/1‑150 (MQ=255)
                                                                                                                              ccAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCa                         >  5:2008889/1‑150 (MQ=255)
                                                                                                                               cAAGCGGTAAGGCACCGGATTCTTATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAAt                       <  2:1480095/151‑1 (MQ=255)
                                                                                                                               cAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAATCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAAt                       <  7:773567/151‑1 (MQ=255)
                                                                                                                                aaGCGGTAAGGCACCGGATTCTTATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATg                      <  2:410446/151‑1 (MQ=255)
                                                                                                                                 aGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGt                     >  6:1813119/1‑151 (MQ=255)
                                                                                                                                    ggTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGTCgg                  >  8:658199/1‑151 (MQ=255)
                                                                                                                                    ggTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAAAGTCgg                  <  2:1933997/151‑1 (MQ=255)
                                                                                                                                      tAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGATCGCTTCGTAGAACTTTATGCTTTTATGCGTTAATACAATCTCTAATGCAATGTTGACACATATTATCATTCAAAGtatga                 >  4:1749855/1‑146 (MQ=18)
                                                                                                                                       aaGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGGTGTTGACACGTCTTATCCTTCAATGTCGGAt                <  6:1333525/150‑1 (MQ=255)
                                                                                                                                       aaGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGTCGGATg               >  5:1602411/1‑151 (MQ=255)
                                                                                                                                        aGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCTCGTACCCCAGCCAAATTAAAAAAGCTCGCTACGGCGAGCTTTTTGCTTTTCTGCGTTAATACAATCTCGAATGCGATGTAGACTCGTCTTATCATTCAATGACGGATGc              >  4:693620/1‑151 (MQ=21)
                                                                                                                                        aGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGTCGGATGc              <  1:167195/151‑1 (MQ=255)
                                                                                                                                           cACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCCTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGTCGGATGCGAc           <  6:1937597/151‑1 (MQ=255)
                                                                                                                                            aCCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGTCGGATGCGACg          <  7:156455/151‑1 (MQ=255)
                                                                                                                                              cGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGTCGGATGCGACGCt        >  5:1268222/1‑151 (MQ=255)
                                                                                                                                               ggATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGTCGGATGCGACGCtt       >  2:1047103/1‑150 (MQ=255)
                                                                                                                                               ggATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGTCGGATGCGACGCTg       >  7:1661038/1‑151 (MQ=255)
                                                                                                                                               ggATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGTCGGATGCGACGCTg       >  5:1902051/1‑151 (MQ=255)
                                                                                                                                               ggATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGTCGGATGCGACGCTg       >  7:1394521/1‑151 (MQ=255)
                                                                                                                                                 aTTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGTCGGATGCGACGCTGcc     >  3:150353/1‑151 (MQ=255)
                                                                                                                                                 aTTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGTCGGATGCGACGCTGcc     <  8:1052330/151‑1 (MQ=255)
                                                                                                                                                  ttCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCt                                                                            <  4:1356500/79‑1 (MQ=255)
                                                                                                                                                   tCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGTCGGATGCGACGCTGCcg    <  4:943501/150‑1 (MQ=255)
                                                                                                                                                   tCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAAAAAAGCTCGATTCCTCGAGATTTATGCATTTATGCGTTACTACAATCTCGAATGCGATGTAGACTCGTAGAATCATTCAATGTCTCATACGACGCTccccc   >  6:140832/1‑149 (MQ=12)
                                                                                                                                                   tCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAAAAAAGATCGCTTCGGCGAGCTTTAAGCATTTCTGCGTTAATTCAATCTCGAATGCGAAGTAGACACGTCATATCCTTCAAAGTCTCATGccaacctcccgc   >  6:150079/1‑140 (MQ=21)
                                                                                                                                                   tCTATTTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGTCGGATGCGACGCTGCcg    <  6:749653/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
TTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTCATTCAATGTCGAATGCGATGTTGACACGTCTTATCCTTCAATGTCGGATGCGACGCTGCCGCG  >  DH1/3184499‑3184797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑