Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,882,7340AG54.2% 139.0 / inf 501Q76Q (CAA→CAGettAenergy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base A (97/132);  new base G (132/139);  total (229/272)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-02

Only 100 of 503 total aligned reads displayed.

GAGTTTCTTCCCTGGGGCAAAAATTGGTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTGACAAGCTGGCCGCTGAACAAGGC  >  DH1/3882587‑3882884
                                                                                                                                                   |                                                                                                                                                      
gAGTTTCTTCCCTGGGGCAAAAATTGGTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGaa                                                                                                                                                     <  7:308207/151‑1 (MQ=255)
gAGTTTCTTCCCTGGGGCAAAAATTGGTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGa                                                                                                                                                      <  5:850419/150‑1 (MQ=255)
 aGTTTCTTCCCTGGGGCAAAAATTGGTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAAc                                                                                                                                                    <  6:395668/151‑1 (MQ=255)
    ttcttcCCTGGGGCAAAAATTGGTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCg                                                                                                                                                  <  7:698998/150‑1 (MQ=255)
     tcttcCCTGGGGCAAAAATTGGTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGc                                                                                                                                                 >  3:398604/1‑150 (MQ=255)
     tcttcCCTGGGGCAAAAATTGGTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGc                                                                                                                                                 >  3:1797808/1‑150 (MQ=255)
        tcCCTGGGGCAAAAATTGGTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCa                                                                                                                                                <  5:1818870/148‑1 (MQ=255)
           cTGGGGCAAAAATTGGTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGa                                                                                                                                           >  3:958043/1‑150 (MQ=255)
             ggggCAAAAATTGGTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAAcc                                                                                                                                        >  6:1850343/1‑151 (MQ=255)
               ggCAAAAATTGGTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCg                                                                                                                                      <  1:811832/151‑1 (MQ=255)
                gCAAAAATTGGTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCgg                                                                                                                                     >  5:1754346/1‑151 (MQ=255)
                gCAAAAATTGGTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCgg                                                                                                                                     >  3:1841272/1‑151 (MQ=255)
                       ttGGTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAacacac                                                                                                                              >  1:524052/1‑151 (MQ=255)
                       ttGGTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAacacac                                                                                                                              >  3:100905/1‑151 (MQ=255)
                         ggTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACAcc                                                                                                                             <  3:1380802/150‑1 (MQ=255)
                         ggTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACcg                                                                                                                            >  5:550137/1‑151 (MQ=255)
                              ccTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACcgtgcgt                                                                                                                       <  5:1588139/151‑1 (MQ=255)
                              ccTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACcgtgcgg                                                                                                                       >  8:1832279/1‑150 (MQ=255)
                               cTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACcgtgcgtg                                                                                                                      >  4:35044/1‑151 (MQ=255)
                                tGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACcgtgcgtg                                                                                                                      >  5:1982994/1‑150 (MQ=255)
                                gggggCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGa                                                                                                                     <  4:930687/150‑1 (MQ=255)
                                 gggTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCTCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAg                                                                                                                    <  6:802537/151‑1 (MQ=255)
                                 gggTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACcgtgcgtg                                                                                                                      >  2:603898/1‑149 (MQ=255)
                                  ggTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGt                                                                                                                   >  2:1782574/1‑151 (MQ=255)
                                  ggTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGt                                                                                                                   <  8:1759478/151‑1 (MQ=255)
                                      tGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCAt                                                                                                               >  1:1805525/1‑151 (MQ=255)
                                       gAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCa                                                                                                                <  4:666598/149‑1 (MQ=255)
                                           ggCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTga                                                                                                            >  6:1431670/1‑149 (MQ=255)
                                           ggCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTgaag                                                                                                          <  3:868583/151‑1 (MQ=255)
                                              gcgGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGaagaag                                                                                                       <  8:1525724/151‑1 (MQ=255)
                                               cgGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGc                                                                                                      <  8:1198975/151‑1 (MQ=255)
                                                gggTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCg                                                                                                     >  1:613193/1‑151 (MQ=255)
                                                 ggTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCgg                                                                                                    <  5:1195891/151‑1 (MQ=255)
                                                 ggTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCgg                                                                                                    >  2:676678/1‑151 (MQ=255)
                                                 ggTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCgg                                                                                                    <  5:266206/151‑1 (MQ=255)
                                                    aaGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGttt                                                                                                 <  8:677198/151‑1 (MQ=255)
                                                    aaGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGttt                                                                                                 <  6:1634380/151‑1 (MQ=255)
                                                    aaGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGttt                                                                                                 <  7:476392/151‑1 (MQ=255)
                                                    aaGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGGCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGtt                                                                                                  <  4:1625015/150‑1 (MQ=255)
                                                      gTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCa                                                                                               <  2:202730/151‑1 (MQ=255)
                                                           cccTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGt                                                                                          <  3:1259759/151‑1 (MQ=255)
                                                            ccTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTg                                                                                         >  3:27302/1‑151 (MQ=255)
                                                              tgctgcGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGt                                                                                       >  6:97440/1‑151 (MQ=255)
                                                                 tgcGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGCGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTa                                                                                     >  6:1686138/1‑150 (MQ=255)
                                                                 tgcGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGGTTCAGAAGTGGTTa                                                                                     >  6:1066768/1‑150 (MQ=255)
                                                                  gcgcATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTa                                                                                     <  7:1606433/149‑1 (MQ=255)
                                                                    gcATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCCGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAAAGc                                                                                 >  7:1507717/1‑151 (MQ=255)
                                                                       ttATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCt                                                                              >  1:1879196/1‑151 (MQ=255)
                                                                         aTGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTg                                                                             >  6:1123911/1‑150 (MQ=255)
                                                                         aTGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGa                                                                            <  5:394703/151‑1 (MQ=255)
                                                                         aTGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTg                                                                             <  5:660877/150‑1 (MQ=255)
                                                                          tGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGa                                                                            >  8:396582/1‑150 (MQ=255)
                                                                           ggCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGaa                                                                           >  4:492554/1‑150 (MQ=255)
                                                                            gCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAAc                                                                         <  1:710548/151‑1 (MQ=255)
                                                                             cGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACg                                                                        >  1:2008438/1‑151 (MQ=255)
                                                                                gCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCt                                                                     <  7:1112890/151‑1 (MQ=255)
                                                                                gCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCt                                                                     <  1:136676/151‑1 (MQ=255)
                                                                                 cATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTGTCAGAAGTGGTTAACGCCCTGAAACGCCTg                                                                    >  2:1012065/1‑151 (MQ=255)
                                                                                  aTTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTgg                                                                   <  3:586772/151‑1 (MQ=255)
                                                                                    tGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGAt                                                                 >  4:1136524/1‑151 (MQ=255)
                                                                                      aTAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGa                                                               <  6:75294/151‑1 (MQ=255)
                                                                                         aaGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAgt                                                            >  8:19114/1‑151 (MQ=255)
                                                                                           gACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAgtgt                                                          >  1:1591722/1‑151 (MQ=255)
                                                                                            aCATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTa                                                         <  3:622407/151‑1 (MQ=255)
                                                                                                cGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATgc                                                      <  2:898267/150‑1 (MQ=255)
                                                                                                 gAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATgcgc                                                    >  2:484473/1‑151 (MQ=255)
                                                                                                  aaGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCt                                                   >  2:1927618/1‑151 (MQ=255)
                                                                                                  aaGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCt                                                   >  8:194896/1‑151 (MQ=255)
                                                                                                   aGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTg                                                  <  4:1421991/151‑1 (MQ=255)
                                                                                                       gAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAAACGCAGCTGAACACGGAAAACACCGTGATTGAGTCCATTTAAGAAGAGGTTTAAGAAGTGTTTAACGCACTGAAACGCCTGGATGAAGTGTATGCGATGTACg                                              >  8:246295/1‑151 (MQ=255)
                                                                                                        aaGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGc                                             <  6:1589233/151‑1 (MQ=255)
                                                                                                        aaGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGc                                             >  2:1233100/1‑151 (MQ=255)
                                                                                                         aGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGcc                                            <  6:313032/151‑1 (MQ=255)
                                                                                                              gTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATcc                                       <  4:766461/151‑1 (MQ=255)
                                                                                                               tCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGTTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCg                                      >  4:1696892/1‑151 (MQ=255)
                                                                                                                 cGCAGCCAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCgg                                     >  3:1976199/1‑150 (MQ=255)
                                                                                                                       cAGACATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCg                               >  7:921838/1‑150 (MQ=255)
                                                                                                                         gACATCAAGATTGGTTATCTGCCGCAAGACCCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGAtt                            <  7:1910780/151‑1 (MQ=255)
                                                                                                                         gACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGAtt                            <  3:1133236/151‑1 (MQ=255)
                                                                                                                          aCATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGAttt                           <  5:1383954/151‑1 (MQ=255)
                                                                                                                          aCATCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGAttt                           >  4:1239394/1‑151 (MQ=255)
                                                                                                                           cATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGAtttt                          <  8:1302083/151‑1 (MQ=255)
                                                                                                                           cATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGAtttt                          >  2:1631848/1‑151 (MQ=255)
                                                                                                                            aTCAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTg                         >  1:1445114/1‑151 (MQ=255)
                                                                                                                            aTCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTg                         >  8:1092997/1‑151 (MQ=255)
                                                                                                                             tCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTg                         >  3:1465407/1‑150 (MQ=255)
                                                                                                                              cAAGATTGGTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAACTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTGAc                       >  4:157262/1‑151 (MQ=255)
                                                                                                                                 gATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTGACAAg                    <  3:730325/151‑1 (MQ=255)
                                                                                                                                  aTTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTGACAAGc                   <  7:2017123/151‑1 (MQ=255)
                                                                                                                                  aTTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTGACAAGc                   <  6:221106/151‑1 (MQ=255)
                                                                                                                                    tGGTTATCTGCGGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTGACAAGCt                  <  1:102506/150‑1 (MQ=255)
                                                                                                                                     gtttAGCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGCTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTGACAAGCTg                 <  4:1994163/148‑1 (MQ=255)
                                                                                                                                      gTTATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTGACAAGCTGGc               >  6:1230495/1‑151 (MQ=255)
                                                                                                                                        tATCTGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTGACAAGCTGGcc              <  1:927498/150‑1 (MQ=255)
                                                                                                                                           cTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTGACAAGCTGGCCGCTg          >  3:1668684/1‑151 (MQ=255)
                                                                                                                                            tGCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTGACAAGCTGGCCGCTg          >  7:1383823/1‑150 (MQ=255)
                                                                                                                                             gCCGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTGACAAGCTGGCCGCt           <  4:550782/148‑1 (MQ=255)
                                                                                                                                             gCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTGACAAGCTGGCCGCt           <  7:1274441/148‑1 (MQ=255)
                                                                                                                                               cGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTGACAAGCTGGCCGCTGAACa      <  8:52066/151‑1 (MQ=255)
                                                                                                                                               cGCAGGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTGACAAGCTGGCCGCTGAACaa     <  2:1346671/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
GAGTTTCTTCCCTGGGGCAAAAATTGGTGTCCTGGGTCTGAATGGCGCGGGTAAGTCCACCCTGCTGCGCATTATGGCGGGCATTGATAAAGACATCGAAGGTGAAGCGCGTCCGCAGCCAGACATCAAGATTGGTTATCTGCCGCAAGAACCGCAGCTGAACCCGGAACACACCGTGCGTGAGTCCATTGAAGAAGCGGTTTCAGAAGTGGTTAACGCCCTGAAACGCCTGGATGAAGTGTATGCGCTGTACGCCGATCCGGATGCCGATTTTGACAAGCTGGCCGCTGAACAAGGC  >  DH1/3882587‑3882884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: