Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,948,6440AG52.2% 74.9 / inf 540N102S (AAC→AGC) yjiLhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (133/125);  new base G (136/146);  total (269/271)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.91e-02

Only 100 of 564 total aligned reads displayed.

AGACAACGCCGTTTCTGACGCTCACCGGCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGACAGCATTACCGAAAATGTCAC  >  DH1/3948494‑3948794
                                                                                                                                                      |                                                                                                                                                      
       gCCGTTTCTGACGCTCACCGGCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTg                                                                                                                                                 <  6:1202563/151‑1 (MQ=255)
        ccGTTTCTGACGCTCAGCGTGTACGGGCGGCAACTGGTGGATTTTGCCGATAAAGATGTAACGGAAATCTCCTGTCACGTGCTGGGCGGACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGa                                                                                                                                                <  7:1958455/151‑1 (MQ=255)
           tttCTGACGCTCACCGGCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTc                                                                                                                                             >  7:1042078/1‑151 (MQ=255)
            ttCTGACGCTCACCGGCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTc                                                                                                                                             <  3:1266909/150‑1 (MQ=255)
             tCTGACGCTCACCGGCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTc                                                                                                                                             >  5:820171/1‑149 (MQ=255)
              cTGACGCTCACCGGCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGc                                                                                                                                          >  3:86459/1‑151 (MQ=255)
               tGACGCTCACCGGCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCt                                                                                                                                         >  7:1431986/1‑151 (MQ=255)
                gACGCTCACCGGCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCtt                                                                                                                                        >  3:461983/1‑151 (MQ=255)
                gACGCTCACCGGCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCtt                                                                                                                                        >  8:434490/1‑151 (MQ=255)
                gACGCTCACCGGCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAg                                                                                                                                           <  3:1448441/148‑1 (MQ=255)
                gACGCTCACCGGCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCtt                                                                                                                                        >  3:611731/1‑151 (MQ=255)
                  cGCTCACCGGCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGATTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTtg                                                                                                                                       >  2:358570/1‑150 (MQ=255)
                       aCCGGCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTtgatgatg                                                                                                                                 >  1:1377362/1‑151 (MQ=255)
                       aCCGGCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTtgatgatg                                                                                                                                 >  7:815827/1‑151 (MQ=255)
                         cGGCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGAc                                                                                                                               >  4:815808/1‑151 (MQ=255)
                          ggCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACg                                                                                                                              <  1:368327/151‑1 (MQ=255)
                              aCGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTCATGATGACGGTaa                                                                                                                          <  1:1924201/151‑1 (MQ=255)
                               cGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAAc                                                                                                                         >  8:1713938/1‑151 (MQ=255)
                               cGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAAc                                                                                                                         <  1:533427/151‑1 (MQ=255)
                                    ggcAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCtgtg                                                                                                                    <  7:1613710/151‑1 (MQ=255)
                                     gcAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGc                                                                                                                   <  2:1050309/151‑1 (MQ=255)
                                     gcAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGc                                                                                                                   <  7:278209/151‑1 (MQ=255)
                                      cAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCg                                                                                                                  <  6:1982007/151‑1 (MQ=255)
                                       aaCTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGGGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTACCCTGTGCg                                                                                                                  >  2:84472/1‑150 (MQ=255)
                                       aaCTGGTGGATTTTGCAGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGa                                                                                                                 <  8:70574/151‑1 (MQ=255)
                                                aTTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACTGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATAGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTCACCTGTGCGATTTCAtgat                                                                                                        >  1:212661/1‑151 (MQ=255)
                                                aTTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCtgat                                                                                                        >  4:1045466/1‑151 (MQ=255)
                                                 ttttGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGTTTGATGATGACGGTAACCTGTGCGATTTCCtgatg                                                                                                       >  5:1474464/1‑151 (MQ=255)
                                                  tttGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCtgatg                                                                                                       <  8:1810816/150‑1 (MQ=255)
                                                   ttGCCGATAAACAGGTAACGGAAATCTCCTGTCCCGGGCTGGGCGCACGGTTTCTTGCGCCCGCAACGCGCGCGGTAATCGACATCGGTGGTCACGAGAGAAAAGTGCTTCAGCTTTATGCTGACGGTAACCTGTGCGATTACCtgatga                                                                                                      >  4:360881/1‑150 (MQ=255)
                                                   ttGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCtgatga                                                                                                      >  4:992685/1‑150 (MQ=255)
                                                   ttGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCtgatga                                                                                                      >  5:732286/1‑150 (MQ=255)
                                                   ttGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCtgatga                                                                                                      <  3:1705445/150‑1 (MQ=255)
                                                    tGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGatgaat                                                                                                    >  8:492119/1‑151 (MQ=255)
                                                    tGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGatgaat                                                                                                    >  5:390826/1‑151 (MQ=255)
                                                      ccGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGatgaatga                                                                                                  <  3:1823160/151‑1 (MQ=255)
                                                      ccGATAAACACGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTAGCGCCAGCCACGCGCGCGGTAATCGACATCGCTGGTCAGGACAACAAAGGGATTCAGCTTGATGATGACGGTAACCTGTGCGATATCCTGatgaatg                                                                                                   >  6:708102/1‑150 (MQ=255)
                                                       cGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGAc                                                                                                 <  5:256567/151‑1 (MQ=255)
                                                           aaaCAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAAt                                                                                             >  8:1599671/1‑151 (MQ=255)
                                                           aaaCAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAAt                                                                                             >  7:411331/1‑151 (MQ=255)
                                                           aaaCAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAAt                                                                                             <  5:1753757/151‑1 (MQ=255)
                                                            aaCAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATg                                                                                            <  7:7138/151‑1 (MQ=255)
                                                             aCAGGTAACGGAAATCTCCTGTCCCGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATgc                                                                                           <  5:1566903/151‑1 (MQ=255)
                                                              cAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATgcg                                                                                          <  1:711158/151‑1 (MQ=255)
                                                               aGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATgcgc                                                                                         >  2:1728221/1‑151 (MQ=255)
                                                                  tAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCgaggc                                                                                      >  7:1881784/1‑151 (MQ=255)
                                                                    aCGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGcggcgg                                                                                    <  8:35758/151‑1 (MQ=255)
                                                                      ggAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGc                                                                                  <  3:1097954/151‑1 (MQ=255)
                                                                       gAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCa                                                                                 <  2:1292378/151‑1 (MQ=255)
                                                                        aaaTCTCCTGTCCCGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGGGGGCAc                                                                                <  6:805601/151‑1 (MQ=255)
                                                                        aaaTCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGGGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCAc                                                                                <  8:863913/151‑1 (MQ=255)
                                                                             tcCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGc                                                                           >  7:1167817/1‑151 (MQ=255)
                                                                             tcCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGc                                                                           >  1:928524/1‑151 (MQ=255)
                                                                              ccTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGc                                                                           <  3:1511730/150‑1 (MQ=255)
                                                                              ccTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGc                                                                           <  3:294892/150‑1 (MQ=255)
                                                                               cTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACGAATGCGCGGCGGGCACCGGGCGt                                                                         >  1:1537734/1‑151 (MQ=255)
                                                                               cTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGt                                                                         >  5:192525/1‑151 (MQ=255)
                                                                                 gTCACGGGCTGGGCGCACGTTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGt                                                                         <  2:1841582/149‑1 (MQ=255)
                                                                                  tCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCCCCGGGCGTTTc                                                                      >  6:1514108/1‑151 (MQ=255)
                                                                                   cACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTcc                                                                     <  2:1618678/151‑1 (MQ=255)
                                                                                   cACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTcc                                                                     <  3:1989088/151‑1 (MQ=255)
                                                                                    aCGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCt                                                                    >  2:28430/1‑151 (MQ=255)
                                                                                    aCGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCt                                                                    >  1:1007841/1‑151 (MQ=255)
                                                                                    aCGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCt                                                                    <  6:1800082/151‑1 (MQ=255)
                                                                                               gcACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGAtct                                                         <  4:1596622/151‑1 (MQ=255)
                                                                                                  cGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTcgc                                                      >  4:1718106/1‑151 (MQ=255)
                                                                                                  cGGTTTCTTGCGCAAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTcgc                                                      >  6:171374/1‑151 (MQ=255)
                                                                                                   ggTTTCTTGCGCCAGCAACGCTCGCGGTAATGGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTcgcg                                                     <  5:460862/151‑1 (MQ=255)
                                                                                                      ttCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCAc                                                  >  8:1092121/1‑151 (MQ=255)
                                                                                                           gcgcCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTg                                             >  2:526107/1‑151 (MQ=255)
                                                                                                             gcCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGc                                           >  6:241257/1‑151 (MQ=255)
                                                                                                              ccAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCa                                          <  4:1934383/151‑1 (MQ=255)
                                                                                                              ccAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGc                                           >  5:1337693/1‑150 (MQ=255)
                                                                                                              ccAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCa                                          <  4:615304/151‑1 (MQ=255)
                                                                                                                aGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGCTGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCCCCGGGCCTTTCCTGGAGGGGATCTCGCGCACGCTTGGCAcc                                        >  2:1432063/1‑151 (MQ=255)
                                                                                                                  cAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAg                                      <  7:982235/151‑1 (MQ=255)
                                                                                                                   aaCGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGc                                     >  4:1396815/1‑151 (MQ=255)
                                                                                                                     cgcgcccgGTAATAGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATCACGGTAACCTGTCCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGt                                   >  4:1815730/1‑151 (MQ=255)
                                                                                                                      gcgcgcgGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTc                                  >  5:990413/1‑151 (MQ=255)
                                                                                                                         cgcgGTAATCGACATCGTGGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAg                               <  4:26016/151‑1 (MQ=255)
                                                                                                                          gcgGTAAGCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTGGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGGGCACGCTTGGCACCAGCGTCGAGc                              <  2:670274/151‑1 (MQ=255)
                                                                                                                            ggTAATCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACATGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCa                             >  2:110708/1‑150 (MQ=255)
                                                                                                                               aaTCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACTGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTc                         <  7:13756/151‑1 (MQ=255)
                                                                                                                                aTCGACATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCg                        <  6:514197/151‑1 (MQ=255)
                                                                                                                                  cGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGAc                      >  6:375055/1‑151 (MQ=255)
                                                                                                                                   gACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGACa                     <  6:1111669/151‑1 (MQ=255)
                                                                                                                                     cATCGGTGGTCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGACAGc                   <  2:976732/151‑1 (MQ=255)
                                                                                                                                        cGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGACAGCAt                 <  8:1958838/150‑1 (MQ=255)
                                                                                                                                          gtggtCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGACAGCATTAc              >  8:1202058/1‑151 (MQ=255)
                                                                                                                                           tggtCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGGGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACGCGACAGCATTAcc             >  1:1274981/1‑151 (MQ=255)
                                                                                                                                             gtCAGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGACAGCATTACCGa           <  4:730330/151‑1 (MQ=255)
                                                                                                                                             gtCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGACAGCATTACCGa           <  4:1265432/151‑1 (MQ=255)
                                                                                                                                               cAGGACAACAATGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATATCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGACAGCATTACCGaa          >  5:615286/1‑150 (MQ=255)
                                                                                                                                                aGGACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGACAGCATTACCGaaa         >  8:1457195/1‑150 (MQ=255)
                                                                                                                                                 ggACAGCAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGACAGCATTACCGAAAAt       <  7:530342/151‑1 (MQ=255)
                                                                                                                                                 ggACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGACAGCATTACCGAAAAt       <  5:1167172/151‑1 (MQ=255)
                                                                                                                                                   acaacaAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGACAGCATTACCGAAAATGt     >  1:1114360/1‑151 (MQ=255)
                                                                                                                                                     aacaaAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGACAGCATTACCGAAAATGTc    >  6:951935/1‑150 (MQ=255)
                                                                                                                                                      acaaAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGACAGCATTACCGAAAATGTCAc  <  1:315504/151‑1 (MQ=255)
                                                                                                                                                      acaaAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGACAGCATTACCGAAAATGTCAc  <  1:1720199/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
AGACAACGCCGTTTCTGACGCTCACCGGCTACGGGCGGCAACTGGTGGATTTTGCCGATAAACAGGTAACGGAAATCTCCTGTCACGGGCTGGGCGCACGGTTTCTTGCGCCAGCAACGCGCGCGGTAATCGACATCGGTGGTCAGGACAACAAAGTGATTCAGCTTGATGATGACGGTAACCTGTGCGATTTCCTGATGAATGACAAATGCGCGGCGGGCACCGGGCGTTTCCTGGAGGTGATCTCGCGCACGCTTGGCACCAGCGTCGAGCAACTCGACAGCATTACCGAAAATGTCAC  >  DH1/3948494‑3948794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑