Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,328,6930AG52.8% 117.5 / inf 613F143S (TTC→TCC) rpoCDNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base A (135/154);  new base G (155/168);  total (290/323)
Fisher's exact test for biased strand distribution p-value = 8.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01

Only 100 of 620 total aligned reads displayed.

CCATGCTCTTCAGCAGAGCCTGGATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTCACGGCGTACTTTAGTCTGGG  >  DH1/4328544‑4328843
                                                                                                                                                     |                                                                                                                                                      
ccATGCTCTTCAGCAGAGCCT‑GATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAAt                                                                                                                                                      <  3:1152228/151‑1 (MQ=255)
   tGCTCTTCAGCAGAGCCTGGATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTc                                                                                                                                                    >  6:396846/1‑151 (MQ=255)
    gCTCTTCAGCAGAGCCTGGATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTc                                                                                                                                                    <  8:1192054/150‑1 (MQ=255)
       ctTCAGCAGAGCCTGGATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCaa                                                                                                                                                  >  5:1740788/1‑149 (MQ=255)
         tCAGCAGAGCCTGGATTGCTTCCGCCCCCATCTTCGCGGCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAagt                                                                                                                                               <  3:685866/150‑1 (MQ=255)
          cagcagAGCCTGGATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAagtac                                                                                                                                             <  8:7727/151‑1 (MQ=255)
          cagcagAGCCTGGATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAagtac                                                                                                                                             >  2:914847/1‑151 (MQ=255)
          cagcagAGCCTGGATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAagtac                                                                                                                                             >  8:350445/1‑151 (MQ=255)
           agcagAGCCTGGATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAagtaca                                                                                                                                            <  2:1018724/151‑1 (MQ=255)
            gcagAGCCTGGATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAagtacag                                                                                                                                           <  5:284130/151‑1 (MQ=255)
               gagCCTGGATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAagtacagtac                                                                                                                                        >  3:1495088/1‑151 (MQ=255)
                agCCTGGATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTAcg                                                                                                                                       <  4:339783/151‑1 (MQ=255)
                 gCCTGGATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTAcgc                                                                                                                                      <  7:552184/151‑1 (MQ=255)
                     ggATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGtt                                                                                                                                   <  1:1652321/150‑1 (MQ=255)
                     ggATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGt                                                                                                                                    <  5:1481246/149‑1 (MQ=255)
                     ggATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCAGACCGCCTTCGATAACCACATAGGATTCAACGTACAGTACGCGTTc                                                                                                                                  >  1:1415479/1‑151 (MQ=255)
                       aTTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGa                                                                                                                                <  7:1731618/151‑1 (MQ=255)
                          gCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGatat                                                                                                                             >  7:859656/1‑151 (MQ=255)
                          gCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGata                                                                                                                              <  6:1336756/150‑1 (MQ=255)
                             tCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATc                                                                                                                            <  2:1576548/149‑1 (MQ=255)
                              ccGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATcgc                                                                                                                          <  1:338153/150‑1 (MQ=255)
                               cGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCgcgc                                                                                                                        <  2:1747801/151‑1 (MQ=255)
                                gCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCgcgc                                                                                                                        >  2:762332/1‑150 (MQ=255)
                                 cccccATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAg                                                                                                                      <  1:2016063/151‑1 (MQ=255)
                                  ccccATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGc                                                                                                                     >  5:52501/1‑151 (MQ=255)
                                   cccATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCg                                                                                                                    >  5:1623599/1‑151 (MQ=255)
                                    ccATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCgg                                                                                                                   <  3:1836861/151‑1 (MQ=255)
                                     cATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGc                                                                                                                  >  2:339620/1‑151 (MQ=255)
                                        cTTCGCGTCGAATTCGTCCCCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCatt                                                                                                               >  3:1688002/1‑150 (MQ=255)
                                        cTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCat                                                                                                                >  5:1283018/1‑150 (MQ=255)
                                          tCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCatat                                                                                                              <  8:544821/150‑1 (MQ=255)
                                          tCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCatat                                                                                                              <  5:1864071/150‑1 (MQ=255)
                                           cgcgTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCg                                                                                                            <  4:1651845/151‑1 (MQ=255)
                                            gcgTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGa                                                                                                           <  5:781748/151‑1 (MQ=255)
                                                cGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGagcag                                                                                                       <  2:51223/151‑1 (MQ=255)
                                                cGAATTCCTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGAATCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGagc                                                                                                         >  3:1934962/1‑149 (MQ=255)
                                                  aaTTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAgcagca                                                                                                     <  1:62802/151‑1 (MQ=255)
                                                   aTTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGcagcag                                                                                                    <  2:1120455/151‑1 (MQ=255)
                                                       gTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCg                                                                                                <  3:423499/151‑1 (MQ=255)
                                                           ccGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATAc                                                                                            <  8:1709493/151‑1 (MQ=255)
                                                            cGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACg                                                                                           >  3:1060140/1‑151 (MQ=255)
                                                             gAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACgg                                                                                          >  6:770522/1‑151 (MQ=255)
                                                             gAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACgg                                                                                          >  7:960127/1‑151 (MQ=255)
                                                                    tCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGc                                                                                    <  4:1892839/150‑1 (MQ=255)
                                                                      cAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGc                                                                                 <  5:859271/151‑1 (MQ=255)
                                                                         cgcgTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGAt                                                                              >  1:1588939/1‑151 (MQ=255)
                                                                           cgTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGAttt                                                                            >  3:1377959/1‑151 (MQ=255)
                                                                             tCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTc                                                                           >  1:1813633/1‑150 (MQ=255)
                                                                             tCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTc                                                                           >  7:237826/1‑150 (MQ=255)
                                                                                aGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAgg                                                                        >  4:700801/1‑150 (MQ=255)
                                                                                aGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAgg                                                                        >  7:706570/1‑150 (MQ=255)
                                                                                 gATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGaa                                                                      <  5:1040501/151‑1 (MQ=255)
                                                                                 gATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGaa                                                                      <  4:934327/151‑1 (MQ=255)
                                                                                  aTACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGaa                                                                      <  4:839262/150‑1 (MQ=255)
                                                                                    aCTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAAcc                                                                    >  1:1185460/1‑150 (MQ=255)
                                                                                       gCTCTTCAGTCAGGATCTGCTGACGTTCCTGGTTGGTCATACCGCCTTCGATAACCAGATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCTAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAg                                                                  <  4:50481/149‑1 (MQ=255)
                                                                                          ctTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGAtgtg                                                             >  8:1910575/1‑151 (MQ=255)
                                                                                            tcagtcagGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTgcg                                                           >  6:1880003/1‑151 (MQ=255)
                                                                                               gtcagGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAg                                                        <  6:712369/151‑1 (MQ=255)
                                                                                                   ggATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCgg                                                    <  7:1361777/151‑1 (MQ=255)
                                                                                                   ggATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCgg                                                    <  4:592884/151‑1 (MQ=255)
                                                                                                       ctgctgACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGa                                                 >  5:660607/1‑150 (MQ=255)
                                                                                                        tgctgACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGa                                                 >  8:912179/1‑149 (MQ=255)
                                                                                                        tgctgACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGa                                                 >  2:1902845/1‑149 (MQ=255)
                                                                                                        tgctgACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGa                                                 >  4:1124495/1‑149 (MQ=255)
                                                                                                         gctgACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCggg                                                   <  5:935985/146‑1 (MQ=255)
                                                                                                         gctgACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCgggg                                                  <  8:180973/147‑1 (MQ=255)
                                                                                                          ctgACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGcc                                             >  8:1089437/1‑151 (MQ=255)
                                                                                                            gaggTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGAAGCGGCATATCGAGCAGAAGCCAGATACGTGACGGCAGCGATTTGAGGAAGCAGCTGTGCGCAGTCGGGGACGcc                                             >  1:735641/4‑149 (MQ=255)
                                                                                                            gACGTTCCAGGTTGGGCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCa                                            <  1:832838/150‑1 (MQ=255)
                                                                                                               gTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTc                                        <  7:1013306/151‑1 (MQ=255)
                                                                                                                 tCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCg                                       <  6:1084358/150‑1 (MQ=255)
                                                                                                                  ccAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGAt                                     <  8:277937/151‑1 (MQ=255)
                                                                                                                  ccAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGAt                                     <  7:324320/151‑1 (MQ=255)
                                                                                                                   cAGGTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGAtg                                    <  3:544757/151‑1 (MQ=255)
                                                                                                                    aGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGAtgt                                   >  6:247849/1‑151 (MQ=255)
                                                                                                                     ggTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGAtgtg                                  <  5:397585/151‑1 (MQ=255)
                                                                                                                      gTTGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTgg                                 <  2:677958/151‑1 (MQ=255)
                                                                                                                       ttGGTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGc                                >  6:1549879/1‑151 (MQ=255)
                                                                                                                         ggTCATACCGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGccc                              >  4:21759/1‑151 (MQ=255)
                                                                                                                            cATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCAt                            >  6:1705022/1‑150 (MQ=255)
                                                                                                                            cATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCAt                            >  7:1677064/1‑150 (MQ=255)
                                                                                                                                 cGCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTc                      >  8:1527907/1‑151 (MQ=255)
                                                                                                                                 cGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGACGGGGAAGCCAGTTCGATGTGGCCCATACGCTc                      >  5:1536244/1‑151 (MQ=255)
                                                                                                                                  gCCTTCGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTCa                     <  1:1007866/151‑1 (MQ=255)
                                                                                                                                    cTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTCACg                   >  3:1987137/1‑151 (MQ=255)
                                                                                                                                      tCGATAACCACATAGAATTGAAAGTACAGTACGCGTTCGATATCGCGCAGGGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTCACg                   <  4:153423/149‑1 (MQ=255)
                                                                                                                                      tCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTCACg                   <  2:132043/149‑1 (MQ=255)
                                                                                                                                       cGATAACCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTCACGGCg                >  7:1928830/1‑151 (MQ=255)
                                                                                                                                        gATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGCGGAAGCCAGTTCGATGTGGCCCATACCCTCACGGCGt               >  7:1175893/1‑151 (MQ=255)
                                                                                                                                         aTAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTCACGGCGt               >  4:1291967/1‑150 (MQ=255)
                                                                                                                                           aaCCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTCACGGCGTACt            >  8:1341925/1‑151 (MQ=255)
                                                                                                                                           aaCCACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTCACGGCGTACt            >  1:457814/1‑151 (MQ=255)
                                                                                                                                            aCCACATACGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGAGATTTCAGGAACCACATGTGCGCAGTCGGGGAAGCAAGTTCCATGTGGCCCATACGCACACGGCCTACtt           >  6:1765101/1‑151 (MQ=255)
                                                                                                                                             ccACATAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTCACGGCGTACttt          >  8:1988522/1‑151 (MQ=255)
                                                                                                                                             ccACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTCACGGCGTACttt          >  6:1470830/1‑151 (MQ=255)
                                                                                                                                              cacaTAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTCACGGCGTACttt          >  3:1306233/1‑150 (MQ=255)
                                                                                                                                               acaTAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTCACGGCGTACttt          >  6:56041/1‑149 (MQ=255)
                                                                                                                                                caTAGGATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTCACGGCGTACTTTAGt       <  5:194919/151‑1 (MQ=255)
                                                                                                                                                   aGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTCACGGCGTACTTTAGTCTg    >  3:987577/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CCATGCTCTTCAGCAGAGCCTGGATTGCTTCCGCCCCCATCTTCGCGTCGAATTCGTCACCGAACTCTTCCAGCGCGTCCAGATACTGCTCTTCAGTCAGGATCTGCTGACGTTCCAGGTTGGTCATACCGCCTTCGATAACCACATAGAATTCAAAGTACAGTACGCGTTCGATATCGCGCAGCGGCATATCGAGCAGCAGACCGATACGGGACGGCAGCGATTTCAGGAACCAGATGTGCGCAGTCGGGGAAGCCAGTTCGATGTGGCCCATACGCTCACGGCGTACTTTAGTCTGGG  >  DH1/4328544‑4328843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑