Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,547,8150TC54.5% 182.2 / inf 585Y80C (TAC→TGC) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base T (124/141);  new base C (152/166);  total (277/308)
Fisher's exact test for biased strand distribution p-value = 8.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01

Only 100 of 589 total aligned reads displayed.

TTGTTGCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTAC  >  DH1/4547665‑4547965
                                                                                                                                                      |                                                                                                                                                      
 tgttgCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGt                                                                                                                                                        >  6:2037623/1‑150 (MQ=255)
       gggCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGtc                                                                                                                                                 >  1:968961/1‑151 (MQ=255)
        ggCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGtca                                                                                                                                                <  3:1827711/151‑1 (MQ=255)
          cGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGtcatc                                                                                                                                              <  6:1117417/151‑1 (MQ=255)
           gTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCTCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGtcatca                                                                                                                                             <  6:810815/151‑1 (MQ=255)
           gTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGtcatca                                                                                                                                             <  7:543820/151‑1 (MQ=255)
            ttttCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCCCCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGtcatc                                                                                                                                              >  1:465217/1‑149 (MQ=255)
                  ggTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCgg                                                                                                                                      <  7:1447415/151‑1 (MQ=255)
                      tGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCg                                                                                                                                    >  4:1858909/1‑149 (MQ=255)
                        tCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGt                                                                                                                                >  1:1289988/1‑151 (MQ=255)
                         cGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGt                                                                                                                                <  2:284618/150‑1 (MQ=255)
                         cGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGt                                                                                                                                >  8:1572075/1‑150 (MQ=255)
                          gAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGt                                                                                                                                >  1:1869205/1‑149 (MQ=255)
                           aaGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGAGTTGGCTAGGGGAAACGCAGATGTGATCAGGACCGGCGAGGTAgg                                                                                                                             >  4:734161/1‑151 (MQ=255)
                            aGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACGAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGa                                                                                                                            <  6:1499983/151‑1 (MQ=255)
                            aGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGa                                                                                                                            <  7:1985152/151‑1 (MQ=255)
                              ttAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGc                                                                                                                          >  2:798090/1‑151 (MQ=255)
                                 aCTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGAGGGCGAATCTTACCAGAGATGGTATAAACAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGt                                                                                                                       >  3:987273/1‑151 (MQ=255)
                                  cTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTc                                                                                                                      >  3:304475/1‑151 (MQ=255)
                                   ttCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGCCGAGGTAGGAGCTGTCt                                                                                                                     >  3:776419/1‑151 (MQ=255)
                                    tCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCTGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCt                                                                                                                     <  4:234865/150‑1 (MQ=255)
                                    tCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCt                                                                                                                     <  1:1994440/150‑1 (MQ=255)
                                     cGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGc                                                                                                                   <  2:887016/151‑1 (MQ=255)
                                     cGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGc                                                                                                                   >  2:403348/1‑151 (MQ=255)
                                      gTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCCGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGCAATTGGCTAGGGGAAACGTAGATGTCATCAGGACCTGCGAGGTAGGAGCTGTCTGCg                                                                                                                  >  6:1819651/1‑151 (MQ=255)
                                        tAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCgg                                                                                                                 <  1:826447/150‑1 (MQ=255)
                                        tAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCgg                                                                                                                 >  4:536487/1‑150 (MQ=255)
                                          aCTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGACTCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAAc                                                                                                              >  3:473458/1‑151 (MQ=255)
                                          aCTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAAc                                                                                                              <  3:1524108/151‑1 (MQ=255)
                                            tttCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGGACgg                                                                                                            >  4:300497/1‑151 (MQ=255)
                                            tttCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACgg                                                                                                            >  8:1489226/1‑151 (MQ=255)
                                             ttCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACgg                                                                                                            <  1:1005370/150‑1 (MQ=255)
                                             ttCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACgg                                                                                                            <  1:537033/150‑1 (MQ=255)
                                              tCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGATACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGCACgg                                                                                                            >  6:1642801/1‑149 (MQ=255)
                                               cagcagCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACggagg                                                                                                         <  5:296246/151‑1 (MQ=255)
                                                  cagcGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGaa                                                                                                       >  7:1388586/1‑150 (MQ=255)
                                                  cagcGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGaa                                                                                                       >  2:1297175/1‑150 (MQ=255)
                                                  cagcGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGaaa                                                                                                      <  7:1637552/151‑1 (MQ=255)
                                                   agcGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAAc                                                                                                     >  1:786579/1‑151 (MQ=255)
                                                      gcAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCa                                                                                                   >  4:868693/1‑150 (MQ=255)
                                                      gcAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCa                                                                                                   >  4:1197293/1‑150 (MQ=255)
                                                       cAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCaa                                                                                                  >  5:414656/1‑150 (MQ=255)
                                                         aaaTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatc                                                                                               <  1:1093118/151‑1 (MQ=255)
                                                          aaTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatcc                                                                                              <  7:1914912/151‑1 (MQ=255)
                                                          aaTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatcc                                                                                              >  1:682917/1‑151 (MQ=255)
                                                          aaTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatcc                                                                                              <  5:1427903/151‑1 (MQ=255)
                                                                gTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTg                                                                                        <  6:543787/151‑1 (MQ=255)
                                                                gTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTg                                                                                        >  8:1682407/1‑151 (MQ=255)
                                                                  tCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGc                                                                                       <  2:1186797/150‑1 (MQ=255)
                                                                  tCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAACCCAAATCCATCCTGc                                                                                       >  4:459500/1‑150 (MQ=255)
                                                                  tCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGc                                                                                       >  7:1116291/1‑150 (MQ=255)
                                                                   cACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCaa                                                                                     <  5:763084/151‑1 (MQ=255)
                                                                   cACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCa                                                                                      >  5:1606204/1‑150 (MQ=255)
                                                                   cACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCaa                                                                                     <  8:1807288/151‑1 (MQ=255)
                                                                   cACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCa                                                                                      <  1:1497077/150‑1 (MQ=255)
                                                                           ttCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAtctca                                                                              <  2:1625649/150‑2 (MQ=255)
                                                                             gggcgggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGt                                                                           <  6:453289/150‑1 (MQ=255)
                                                                             cggcggGCGCATCTTACCAGAGATGGTATCACCAGTGCGGAGGTGGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGCGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAg                                                                            <  8:1336901/150‑1 (MQ=255)
                                                                               gcgggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTAc                                                                         <  5:1275358/151‑1 (MQ=255)
                                                                                cgggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACg                                                                        >  8:1291009/1‑151 (MQ=255)
                                                                                  ggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGcc                                                                      <  3:1074403/151‑1 (MQ=255)
                                                                                  ggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGcc                                                                      <  1:1413118/151‑1 (MQ=255)
                                                                                   gcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCa                                                                     <  8:2019921/151‑1 (MQ=255)
                                                                                   gcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGcc                                                                      >  5:2027085/1‑150 (MQ=255)
                                                                                   gcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGcc                                                                      >  4:366834/1‑150 (MQ=255)
                                                                                    cgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCAt                                                                    <  2:153737/151‑1 (MQ=255)
                                                                                      aaTCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCa                                                                  <  8:1154771/151‑1 (MQ=255)
                                                                                      aaTCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGAGTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCa                                                                  <  4:1730605/151‑1 (MQ=255)
                                                                                           tACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGTGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCaa                                                              <  1:1203765/150‑1 (MQ=255)
                                                                                           tACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCAc                                                                 <  2:498877/147‑1 (MQ=255)
                                                                                                gagaTGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGatat                                                        >  3:1174102/1‑151 (MQ=255)
                                                                                                 agaTGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATc                                                       <  7:742858/151‑1 (MQ=255)
                                                                                                 agaTGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATc                                                       >  4:1106497/1‑151 (MQ=255)
                                                                                                  gaTGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCt                                                      <  2:1717973/151‑1 (MQ=255)
                                                                                                          cACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACCGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCAct                                              >  8:506763/1‑151 (MQ=255)
                                                                                                          cACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCAct                                              <  3:860274/151‑1 (MQ=255)
                                                                                                              aGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCttt                                          <  7:428250/151‑1 (MQ=255)
                                                                                                               gTGCGGAGGTGGAAACGGCGGATTTGGCTAGGGGATTCGCAGATGTCATCAGGACGGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGATACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATCTCTTCGCCACTCttt                                          <  6:1297973/150‑1 (MQ=255)
                                                                                                                tGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGc                                        >  3:627424/1‑151 (MQ=255)
                                                                                                                 gCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGc                                        <  5:606039/150‑1 (MQ=255)
                                                                                                                     aGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGtgct                                   <  3:1935870/151‑1 (MQ=255)
                                                                                                                      ggTTGAAACTGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGTACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGtgctg                                  <  2:691306/151‑1 (MQ=255)
                                                                                                                         tGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTgctgct                                <  7:339492/150‑1 (MQ=255)
                                                                                                                         tGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCtt                               >  4:1593172/1‑151 (MQ=255)
                                                                                                                         tGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCtt                               >  4:133578/1‑151 (MQ=255)
                                                                                                                          gAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTc                              <  5:1714696/151‑1 (MQ=255)
                                                                                                                              cggcggATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAACCCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAgg                           >  8:693513/1‑150 (MQ=255)
                                                                                                                                  ggATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGc                      <  6:1604238/151‑1 (MQ=255)
                                                                                                                                  ggATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGc                      >  8:357541/1‑151 (MQ=255)
                                                                                                                                   gATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGc                      >  3:1312264/1‑150 (MQ=255)
                                                                                                                                     tttGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCaa                    >  5:1194313/1‑150 (MQ=255)
                                                                                                                                      ttGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCaaa                   <  4:989314/150‑1 (MQ=255)
                                                                                                                                      ttGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCaaa                   <  5:94098/150‑1 (MQ=255)
                                                                                                                                         gCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaata               <  5:840752/151‑1 (MQ=255)
                                                                                                                                          cTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCCTCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaata               >  2:1986858/1‑150 (MQ=255)
                                                                                                                                               ggAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTcc         <  5:1876830/151‑1 (MQ=255)
                                                                                                                                                 aaaCGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTg       >  6:669738/1‑151 (MQ=255)
                                                                                                                                                    cGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCtt    >  7:1527326/1‑151 (MQ=255)
                                                                                                                                                     gTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCtt    >  7:974472/1‑150 (MQ=255)
                                                                                                                                                     gTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTa   <  1:1680166/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
TTGTTGCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTAC  >  DH1/4547665‑4547965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑