Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1236,0860AG52.7% 82.4 / inf 553intergenic (+28/+26)ECDH1_RS01120/gorhypothetical protein/glutathione reductase
Reads supporting (aligned to +/- strand):  ref base A (121/137);  new base G (139/148);  total (262/291)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

Only 100 of 579 total aligned reads displayed.

AAGCGGATGCATTCTCACTCCATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGCCCTGCAACATTTCGTCCATACCAA  >  DH1/235936‑236236
                                                                                                                                                      |                                                                                                                                                      
  gCGGATGCATTCTCACTCCATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAaca                                                                                                                                                      <  4:1667644/151‑1 (MQ=255)
    ggATTCATTCTCACTCCATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTTGGGGCGTTAATTGCGCTGCTGGAACTTATCCGCTTTCTGCTTCAGCTTCTTAACTGATAGCGGAAACGTAATTAAGGGCTAAGAacaca                                                                                                                                                    <  5:1628815/151‑1 (MQ=255)
    ggATGCATTCTCACTCCATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGCCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAacac                                                                                                                                                     >  3:407206/1‑150 (MQ=255)
        gCATTCTCACTCCATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACactac                                                                                                                                                >  8:1395105/1‑151 (MQ=255)
        gCATTCTCACTCCATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACacta                                                                                                                                                 <  5:794089/150‑1 (MQ=255)
           ttCTCACTCCATCGCATGGAGAAAACGGGTGATTGTTAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTActct                                                                                                                                             >  3:183340/1‑151 (MQ=255)
            tctcACTCAATCGCATGGAGAAAACGGGTGATTGATAAACCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTActct                                                                                                                                             <  5:1976347/150‑1 (MQ=255)
              tcACTCCATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCtt                                                                                                                                            >  2:145137/1‑149 (MQ=255)
               cACTCCATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAg                                                                                                                                          <  5:1291982/150‑1 (MQ=255)
               cACTCCATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTa                                                                                                                                           <  4:1296368/149‑1 (MQ=255)
               cACTCCATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAg                                                                                                                                          <  4:1225694/150‑1 (MQ=255)
                   ccATCGCATGGAGAAAACGGGTGATTGATAAAGGAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTGTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGccc                                                                                                                                       <  4:1798473/149‑1 (MQ=255)
                   ccATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCtt                                                                                                                                     >  6:6409/1‑151 (MQ=255)
                   ccATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCtt                                                                                                                                     <  5:563707/151‑1 (MQ=255)
                    cATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCttt                                                                                                                                    <  1:1057433/151‑1 (MQ=255)
                     aTCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGGTCCGCTTTCTGCTTCAGCTTCTGAACTCATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTAAACCCttt                                                                                                                                    >  2:15321/1‑150 (MQ=255)
                     aTCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGATACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCttt                                                                                                                                    >  8:398275/1‑150 (MQ=255)
                     aTCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACAGCCTACTCTTAGCCCttt                                                                                                                                    >  6:361743/1‑150 (MQ=255)
                       cGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAAc                                                                                                                                 <  1:1291183/151‑1 (MQ=255)
                        gCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACa                                                                                                                                <  6:1556915/151‑1 (MQ=255)
                        gCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACa                                                                                                                                <  7:117339/151‑1 (MQ=255)
                        gCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACa                                                                                                                                <  5:1684017/151‑1 (MQ=255)
                        ccATGGAGCACACGGGCGATTGATAAAGCAAGCATCGTTCTAGGCGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACa                                                                                                                                <  2:107072/150‑1 (MQ=255)
                         cATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGACCTTTAACAt                                                                                                                               >  6:883646/1‑151 (MQ=255)
                          aTGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACAtt                                                                                                                              >  3:1337801/1‑151 (MQ=255)
                          aTGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACAtt                                                                                                                              <  2:590388/151‑1 (MQ=255)
                            ttagaAAACGTGTGATTGATAAAGCAATCATCGTTATAGGGTCGTTAATTGCTCTGCTGTANCTGATCCGNTTTCTGCTNNAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACAttt                                                                                                                             <  4:36840/148‑1 (MQ=255)
                                 aaaCGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGATCTGATTGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACTCa                                                                                                                       <  6:1080173/151‑1 (MQ=255)
                                  aaCGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCAt                                                                                                                      >  7:736695/1‑151 (MQ=255)
                                   aCGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCAtt                                                                                                                     <  1:340596/151‑1 (MQ=255)
                                   aCGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCAtt                                                                                                                     >  4:959456/1‑151 (MQ=255)
                                   aCGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCAtt                                                                                                                     >  8:1859732/1‑151 (MQ=255)
                                    cGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTg                                                                                                                    <  5:424589/151‑1 (MQ=255)
                                       gTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTc                                                                                                                  >  1:489637/1‑150 (MQ=255)
                                        tgattgatAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCAc                                                                                                                >  8:17620/1‑151 (MQ=255)
                                         gattgatAAACCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTCAACTCATAGCGTAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCAc                                                                                                                <  1:983448/150‑1 (MQ=255)
                                           ttgatAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCAGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTAACGa                                                                                                              >  3:1179466/1‑150 (MQ=255)
                                            tgatAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGaa                                                                                                             <  1:1492557/150‑1 (MQ=255)
                                             gatAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAAc                                                                                                            <  4:1566772/150‑1 (MQ=255)
                                             gatAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAAct                                                                                                           >  7:410280/1‑151 (MQ=255)
                                             gatAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAAct                                                                                                           <  5:732369/151‑1 (MQ=255)
                                                  aGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACtcttct                                                                                                      <  6:194975/151‑1 (MQ=255)
                                                  aGCAATCATCGTTCTAGCGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATCGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCATAGGCCTTTAACATTTAACGCATTGTCACGAACtcttct                                                                                                      <  6:534682/151‑1 (MQ=255)
                                                     aaTCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTgcc                                                                                                   <  3:614506/151‑1 (MQ=255)
                                                             ttCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTgg                                                                                           >  8:1599610/1‑151 (MQ=255)
                                                               cTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGt                                                                                         >  1:1775486/1‑151 (MQ=255)
                                                                 aGGGTCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGt                                                                                         <  2:38877/149‑1 (MQ=255)
                                                                 aGGGGCGTTAATTGCGCTGCTGGAAGTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGCGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTg                                                                                        <  7:941477/150‑1 (MQ=255)
                                                                 aGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAAGGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTggg                                                                                          <  8:103833/148‑1 (MQ=255)
                                                                   gggCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAAt                                                                                     <  6:1359759/151‑1 (MQ=255)
                                                                    ggCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATg                                                                                    <  5:1912734/151‑1 (MQ=255)
                                                                      cGTTAATTGCGCTGCTGGAACTTATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCTGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGc                                                                                  <  7:1761676/151‑1 (MQ=255)
                                                                      cGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGc                                                                                  <  3:1527549/151‑1 (MQ=255)
                                                                         tAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGAc                                                                               >  6:711983/1‑151 (MQ=255)
                                                                          aaTTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACg                                                                              <  7:1323765/151‑1 (MQ=255)
                                                                             tGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGt                                                                            <  6:1706621/150‑1 (MQ=255)
                                                                                   gctgGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGt                                                                       <  7:1574324/149‑1 (MQ=255)
                                                                                     tgGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGa                                                                    >  1:1607813/1‑150 (MQ=255)
                                                                                      ggAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGa                                                                    >  4:845508/1‑149 (MQ=255)
                                                                                            gATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCttt                                                             <  2:50771/150‑1 (MQ=255)
                                                                                            gATCAGCTTTCTGCTTCAGCTTATTAACTGATAGCGGAAAAGTAATTAAGGGATAAGAACACAATAATCTTAGCCCTTTAACATTTAACGCATTGTCACGAAATCTAATCCCGCCTTTGGGTGAATGGCGACGGTATTGTCGAAGTCtttt                                                            >  4:570432/1‑151 (MQ=255)
                                                                                             aTCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCttttt                                                           <  7:1971228/151‑1 (MQ=255)
                                                                                                 gCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGt                                                       <  2:679600/151‑1 (MQ=255)
                                                                                                  cTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGtt                                                      >  3:1972008/1‑151 (MQ=255)
                                                                                                   tttCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTg                                                     >  2:1793756/1‑151 (MQ=255)
                                                                                                    ttCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTg                                                     <  5:143613/150‑1 (MQ=255)
                                                                                                     tCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGc                                                    <  2:798116/150‑1 (MQ=255)
                                                                                                       tGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGcccc                                                 >  6:1770092/1‑151 (MQ=255)
                                                                                                       tGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGcccc                                                 >  7:282675/1‑151 (MQ=255)
                                                                                                        gCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGccccc                                                >  1:548166/1‑151 (MQ=255)
                                                                                                        gCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGccccc                                                >  5:1059436/1‑151 (MQ=255)
                                                                                                            cAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCt                                            >  5:888847/1‑151 (MQ=255)
                                                                                                             aGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCGCCATCtt                                           >  4:268522/1‑151 (MQ=255)
                                                                                                             aGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGATATTGTCGAAGTCTTTTTTGGTTGCCCCCATCtt                                           <  2:294510/151‑1 (MQ=255)
                                                                                                              gCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCtt                                           >  7:827947/1‑150 (MQ=255)
                                                                                                                   tGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAgcgc                                     >  3:1700462/1‑151 (MQ=255)
                                                                                                                    gAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAgc                                       <  6:1493061/148‑1 (MQ=255)
                                                                                                                    gAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGcc                                    <  8:925213/151‑1 (MQ=255)
                                                                                                                    gAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAgc                                       <  2:903265/148‑1 (MQ=255)
                                                                                                                    gAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTCGCCCCCATCTTCAGCGcc                                    >  5:1227099/1‑151 (MQ=255)
                                                                                                                          aTAGCGGAAACGTCATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCCCGAACTCTTCTGCCGCCGTTGGGTGAGTGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACcgcg                              <  5:1174148/151‑1 (MQ=255)
                                                                                                                          aTAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACcgcg                              <  8:25197/151‑1 (MQ=255)
                                                                                                                          aTAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACcgcg                              <  4:1913823/151‑1 (MQ=255)
                                                                                                                          aTAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACcgcg                              >  3:949396/1‑151 (MQ=255)
                                                                                                                             gCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGa                             >  2:129259/1‑149 (MQ=255)
                                                                                                                             gCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAg                           <  8:824363/151‑1 (MQ=255)
                                                                                                                               ggAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGcc                         >  1:675610/1‑151 (MQ=255)
                                                                                                                                gAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGccc                        <  3:354541/151‑1 (MQ=255)
                                                                                                                                gAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGccc                        >  8:620333/1‑151 (MQ=255)
                                                                                                                                  aaCGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTATCATTTAACGCATTGTCACGAACTATTCTTCCGACGTTGGGTGAATGGAGACGGTTTTGTCGAAGTGTTTTTTTGTTGGCGCCATGTTGAGCGGCACCGCTAAGCCATg                      >  4:1006919/1‑151 (MQ=255)
                                                                                                                                   ccGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGCCCTGc                     >  7:1920903/2‑151 (MQ=255)
                                                                                                                                          tAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGACGCCCTGCAACAttt              >  7:1028203/1‑151 (MQ=255)
                                                                                                                                           aaGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGCCCTGCAACAttt              <  8:1283223/150‑1 (MQ=255)
                                                                                                                                           aaGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGCCCTGCAACATTTc             >  6:2023526/1‑151 (MQ=255)
                                                                                                                                             gggCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGCCCTGCAACATTTCGt           >  2:1656822/1‑151 (MQ=255)
                                                                                                                                             gggCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGCCCTGCAACATTTCGt           <  1:1927671/151‑1 (MQ=255)
                                                                                                                                                 tAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGCCCTGCAACATTTCGTCCAt       >  5:675063/1‑151 (MQ=255)
                                                                                                                                                  aaGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGCCCTGCAACATTTCGTCCa        <  6:1251234/149‑1 (MQ=255)
                                                                                                                                                   agagCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGCCCTGCAACATTTCGTCCATAc     <  5:295959/151‑1 (MQ=255)
                                                                                                                                                   agagCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGCCCTGCAACATTTCGTCCATAc     <  1:852004/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
AAGCGGATGCATTCTCACTCCATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGCCCTGCAACATTTCGTCCATACCAA  >  DH1/235936‑236236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: