Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1677,3280GA42.2% 210.4 / 108.5 166intergenic (‑31/‑179)ttdA/ttdRtartrate dehydratase subunit alpha/transcriptional activator TtdR
Reads supporting (aligned to +/- strand):  ref base G (80/16);  new base A (0/70);  total (80/86)
Fisher's exact test for biased strand distribution p-value = 1.29e-31
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 169 total aligned reads displayed.

TTTAGCTGTTTTAGTTTATCCACCACGTCATCAGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAGGGAATGGCAATGTTTTGCGAAATACACT  >  DH1/677179‑677477
                                                                                                                                                       |                                                                                                                                                      
tttAGCTGTTTTAGTTTATCCACCACGTCATCAGGCATTCGGGTAGAAATCATGGCGGTAAAGGTAGCGACAATCTCTTTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCCCATCATGGCAACTCCA‑G‑ATTTTTTATTTGTCTgg                                                                                                                                                        >  1:1232452/1‑150 (MQ=255)
  tAGCTGTTTTAGTTTATCCACCACGTCATCAGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGGTTATTAC‑TTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGaaa                                                                                                                                                     <  2:1072508/150‑1 (MQ=255)
  tAGCTGTTTTAGTTTATCCACCACGTCATCAGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTTGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTTGaa                                                                                                                                                      >  1:2233207/1‑150 (MQ=255)
   aGCTGTTTTAGTTTATCCACCACGTCATCAGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGaaaa                                                                                                                                                    <  2:1472945/151‑1 (MQ=255)
       gTTTTAGTTTATCCACCACGTCATCAGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑tt                                                                                                                                                <  2:2101153/150‑1 (MQ=255)
         tttAGTTTATCCACCACGTCATCAGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGTGACAAACCCTGTCAACTTATTGACTTGCTGTTGGTTGTTACTTTCGCTCATCATGGTCGCACCA‑G‑TTTTTGTGTTTGTCTTGAAAAA‑tt                                                                                                                                                >  1:1005375/1‑148 (MQ=255)
         tttAGTTTATCCACCACGTCATCAGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑tt                                                                                                                                                <  2:1005376/148‑1 (MQ=255)
         tttAGTTTATCCACCACGTCATCAGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCa                                                                                                                                              <  1:1397809/150‑1 (MQ=255)
          ttAGTTTATCCACCACGTCATCAGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCAc                                                                                                                                             <  1:725809/150‑1 (MQ=255)
               ttATCCACCACGTCATCAGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCca                                                                                                                                        <  2:1899932/150‑1 (MQ=255)
                tATCCACCACGTCATCAGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCcac                                                                                                                                       >  2:1795328/1‑150 (MQ=255)
                   ccaccaCGTCACCAGGCATTGGGGTAAAAATCATGGCGGTAAAGTTAGAAAAAATCCCTGTCAACTTATTCCCGGCTTGTTGCTTATTGCTTCCGCTCACCA‑GGCCACGCCAGG‑TGTTTTGAGTTGTCGGAAAAAA‑TTCACCGCCACAAAc                                                                                                                                   <  1:1726210/151‑1 (MQ=255)
                     accacGTCATCAGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGaa                                                                                                                                                      <  2:1578887/131‑1 (MQ=255)
                     accacGTCATCAGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGAGACAATCTCTGTCAAATTTTTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTTTATTTTTCTGGaa                                                                                                                                                      >  1:1578882/1‑131 (MQ=255)
                       cacGTCATCAGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTAcc                                                                                                                               >  2:44528/1‑151 (MQ=255)
                           tcatcaGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTTTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACt                                                                                                                            >  2:1637703/1‑150 (MQ=255)
                            catcaGGCTTTGGGGTAAAAATCAGGGGGGTAAAGTTAGCGACAATCTCTTTCAACTTATTCCCTGCCTTTTTTTTATTACTTTCGCTCATCATGGCAACTCCA‑T‑TTTTTGTTTTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTaa                                                                                                                          <  2:1675356/151‑1 (MQ=255)
                            catcaGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCAACGCCACAAACTACCACTa                                                                                                                           >  2:2453360/1‑150 (MQ=255)
                            cataaggatttCGGGAAAAAATCAGGGCGGTAAAGTTAGCAACAATCTCTGTAAATTTTTTCCCTCCCTGTTGTTTATTACTTCCGCTCACCATGGCACCTCCA‑T‑TTTTTTTTTTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTa                                                                                                                           <  2:888737/141‑1 (MQ=255)
                             atcaGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTaa                                                                                                                          >  2:2386141/1‑150 (MQ=255)
                               caGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTTCTTATTTCTTTCGCTCATCATGGCAACTCCC‑G‑TTTTTTTATTTTTCTGGAAAAA‑TTCACCGCCACAAACTACCACTaaaa                                                                                                                        >  2:508439/1‑150 (MQ=255)
                                aGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAAt                                                                                                                      >  2:2511865/1‑151 (MQ=255)
                                aGGAATTGGGGTAGAAATCATGGGGTTAAATTAAGCACCATTCTTTGTAAATTTATCCCCTGCTTTTTGTTTATTTCTTCCGCTCATCAGGACAACCCCA‑T‑TTTTTGAATTTGTCGGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAAt                                                                                                                      <  1:1795323/151‑1 (MQ=255)
                                  gCATTCGGGTAGAAATCATGGCGGTAAAGGTAGAGACAATCTCTGTCAACTTATTTACTGCCTGTTGCTTTTTACTTTTGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTTTCTGGAAAAA‑TTTACCGCCACAAACTACCACTAAAAAtgt                                                                                                                    >  1:1415627/1‑151 (MQ=255)
                                   cATTGGGGTAAAAATCATGGCGTAAAATTTAGCAACAATCTCTGTCATCTTTTTCTCTGCCTGTTGTTTTTTTCTTTCGCTCATCATGGCAACTCCA‑T‑TTTTTGTTTTTGTCGGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAAtgtg                                                                                                                   <  1:457273/151‑1 (MQ=255)
                                       cGGGTAAAAATCATGAGGAAAAATTTAGCGCCATCCTCTTCCATTTTTTACCCGCCCTTTTCCTTATTTCTTTCGCCCATTATGGCACCCCCA‑T‑TTTTTTTTTTTGTCGAAAAAAC‑TGCTCCGCCACAAACTACCACTAAAAATGTGGtt                                                                                                                <  1:1073741/150‑1 (MQ=255)
                                         ggTAAAAATCATGGCGGAAAATTTAGCGAAATTCTCTTTAAACTTTTTCCCTGCCTGTTGTTTATTCCTTCCCCTCATCATGGCACCTCCA‑G‑TTTTTTTTTTTGTTTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTta                                                                                                             <  2:939569/151‑1 (MQ=255)
                                          gTAGAAATCTTGGGGGTAAAGTGACCGACATTCTCTTTCAATTTTTCCACTGCTTGTTTTTTATTATTTCCCCTCATCATGGCAACCCCA‑T‑TTTTTTTTTTTGT‑TGGAAAAATTTCACCGCCACAAACTACCACTAAAAATGTGGTTTtat                                                                                                            <  1:2407208/151‑1 (MQ=255)
                                          gTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAAATACCAATAAAAATGTGGTTTtat                                                                                                            >  1:2284340/1‑151 (MQ=255)
                                            aaaaaTCATGGCGGTAAAGTTAGCGACAATCTCTGTCAATTTTTTCCCTGCCTTTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTTTTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTtata                                                                                                           <  2:2638959/148‑1 (MQ=255)
                                               aaTCATGGGGGTAAAGTTAGCGACATTCTCTGTCACTTTTTTCACTGCCTTTTGTTTTTTTTTTTCCCTCATCTTGGCACCTCCA‑T‑TTTTTTTATTTGTCGGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATAtttt                                                                                                       <  2:2281593/151‑1 (MQ=255)
                                               aaTCATGGGGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTTTTGCTTATTCCTTTCGCTCATCAGGGC‑ACCCCA‑GTTTTTTGTTTTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATAtttt                                                                                                       <  2:1232454/151‑1 (MQ=255)
                                               aaTCAGGGGGGTAAAGTTAGCGACAATCTCTGTCACCTTATTCTCTGCCTTTTGTTTATTACTTTCGCTAATCATGGCAACCCTG‑G‑TTTTTGTATTTGTCGGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATAtttt                                                                                                       <  2:11240/151‑1 (MQ=255)
                                                aTCATGGCGGTAAAGTTAACGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCCTGGCCACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGGTTTATAttttt                                                                                                      >  1:1958934/1‑151 (MQ=255)
                                                     ggCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGcc                                                                                                  >  1:2506026/1‑150 (MQ=255)
                                                      gCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCAc                                                                                                >  1:750060/1‑151 (MQ=255)
                                                       ggaaaaattaaagaaCATTCTCTATAAATTTATTCATTGCTTTTTTTTTTTTCTTTCCCCCCATCAGGAAAACTCCA‑T‑TTTTTGTTTTGGTGGGAAAAAT‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCAcc                                                                                               <  1:508439/137‑1 (MQ=255)
                                                       cGGAAAAGTGAGAGACAATCTATGGAAACTTATTCAATGCCTGTTGCGTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTGAGAGTTTTTGCCAcc                                                                                               >  1:2562759/1‑151 (MQ=255)
                                                           aaaGTTAGCGACAATCTCTGTCACCTTATTCCCTGCTTTTTGCTTATTACTTTCCCTCATCAGGCCAACCCCA‑G‑TTTTTGTATTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGt                                                                                                                 <  2:1543835/129‑1 (MQ=255)
                                                           aaaGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGt                                                                                                                 >  1:1543830/1‑129 (MQ=255)
                                                               ttAGCGACAATCTCTGTCAACTTATTCACTGCTTTTTGCTTTTTACTTTCCCTCATCATGGCAACTCCA‑T‑TTTTTGTATTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAata                                                                                        <  1:2453348/150‑1 (MQ=255)
                                                               ttAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAatat                                                                                       >  1:2297021/1‑151 (MQ=255)
                                                               ttAGCGAAAATCTCTGTCAACTTATTCACTGCTTGTTTCTTATTACTTTCGCTCATCATGGCAACTCAA‑T‑TTTTTTTTTTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAata                                                                                        <  1:1637698/150‑1 (MQ=255)
                                                                 aGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGt                                                                                     >  2:30367/1‑151 (MQ=255)
                                                                     aCAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAAtt                                                                                 >  2:813496/1‑151 (MQ=255)
                                                                      cAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAata                                                                                        >  1:1937093/1‑143 (MQ=255)
                                                                        aTCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAAc                                                                              >  1:1011322/1‑151 (MQ=255)
                                                                         tctcTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAAc                                                                              >  2:2024196/1‑150 (MQ=255)
                                                                            ctGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGtt                                                                          >  1:814588/1‑151 (MQ=255)
                                                                            ctGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGtt                                                                          >  2:2220856/1‑151 (MQ=255)
                                                                              gTCAACTTATTCACTGCTTGTTGTTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTTTTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGtt                                                                          <  2:80460/149‑1 (MQ=255)
                                                                               acaccTTATTCCCTGCCTGTTGTTTATTACTTTCGCTCATCATGCCAACCCCA‑T‑TTTTTTTATTTGTGTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGt                                                                       <  2:1430103/147‑1 (MQ=255)
                                                                                cAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGt                                                                       >  1:1430098/1‑150 (MQ=255)
                                                                                        tCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAAcg                                                              >  1:452832/1‑151 (MQ=255)
                                                                                          ccTGCCTGTTGTTTATTACTTTCGCTCATCATGGCAACTCCA‑T‑TTTTTGTATTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAAcgcg                                                            <  2:452831/150‑1 (MQ=255)
                                                                                            tGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGa                                                           >  2:616545/1‑150 (MQ=255)
                                                                                             gctttttGTTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTTTTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGa                                                           <  2:2297030/144‑1 (MQ=255)
                                                                                                 gttgttTATTACTTTCGCTCATCATGGCAACTCCA‑T‑TTTTTGTATTTGTCGGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATa                                                      <  2:2348448/150‑1 (MQ=255)
                                                                                                 gTTGCTTATTATTTTCGCTCATCATGCCAACTCCA‑T‑TTTTTGTATTTTTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATaa                                                     <  2:1958940/151‑1 (MQ=255)
                                                                                                 gTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATa                                                      >  1:27292/1‑150 (MQ=255)
                                                                                                   tgtttattaCTTTCGCTCATCATGGCACCTCCA‑T‑TTTTTGTTTTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAg                                                                            <  2:1375281/123‑1 (MQ=255)
                                                                                                   tGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAg                                                                            >  1:1375276/1‑126 (MQ=255)
                                                                                                   tGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATa                                                      >  2:1816347/1‑148 (MQ=255)
                                                                                                    gCTTATTATTTCCCCTCAACATGCCACCTCAA‑T‑TTTTTGTATTTGTTTGAAAAAA‑TTCGCCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAga                                                   <  2:1833110/150‑1 (MQ=255)
                                                                                                     cTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAgaat                                                 >  2:855961/1‑151 (MQ=255)
                                                                                                     cTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAgaat                                                 >  1:2056903/1‑151 (MQ=255)
                                                                                                       tattaCTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAgaat                                                 >  2:6602/1‑149 (MQ=255)
                                                                                                       tattaCTTTCCCTCATCATGGCAACTCCA‑G‑TTTTTGTTTTTTTCTAAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTGTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAgaatg                                                <  1:1816342/150‑1 (MQ=255)
                                                                                                        attaCTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGa                                                           >  1:34385/1‑138 (MQ=255)
                                                                                                        attaCTTCCGCTCATCAGGGCACCCCCA‑T‑TTTTTGTTTTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGa                                                           <  2:34385/138‑1 (MQ=255)
                                                                                                            ttttCGTTCATCATGCCAACTCCG‑T‑TTTTTTTTTTTGTCGGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTc                                                                         <  2:2195576/119‑1 (MQ=255)
                                                                                                            cTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTc                                                                         >  1:2195567/1‑120 (MQ=255)
                                                                                                              ttCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTg                                         <  2:914199/150‑1 (MQ=255)
                                                                                                                cGCTCATCATGGCAACTCCA‑T‑TTTTTGTATTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTgcg                                       <  2:1932716/150‑1 (MQ=255)
                                                                                                                cGCTAATCAGGGCAACGCAA‑G‑TTTTTTTATTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTgcg                                       <  1:919173/150‑1 (MQ=255)
                                                                                                                    catcatGGCAACTCCA‑T‑TTTTTGTTTTTGTTTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCaga                                  <  1:2105732/151‑1 (MQ=255)
                                                                                                                    catcatGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGcc                                     >  1:600971/1‑148 (MQ=255)
                                                                                                                     atcatGCAAACCCCA‑T‑TTTTTGTATTTGTCGGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCagag                                 <  2:600972/151‑1 (MQ=255)
                                                                                                                     atcatGACAACTCCA‑T‑TTTTTTTTTTTGTTTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCagag                                 <  1:1137411/151‑1 (MQ=255)
                                                                                                                      cccgatctaccccA‑T‑TTTTTTTTTTTTTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGc                                <  2:1323153/140‑1 (MQ=255)
                                                                                                                       catGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAga                                                   >  1:958400/1‑131 (MQ=255)
                                                                                                                       catGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGc                                >  1:1839233/1‑150 (MQ=255)
                                                                                                                        atgtcacccccA‑G‑TTTTTATTTTTGTCGGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAg                              <  1:2353370/142‑1 (MQ=255)
                                                                                                                         tGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAatat                                           >  2:1426622/1‑137 (MQ=255)
                                                                                                                         gggCAACCCCA‑T‑TTTTTGTTTTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAgg                             <  2:2056911/150‑1 (MQ=255)
                                                                                                                          ggCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAggg                            >  2:2429362/1‑151 (MQ=255)
                                                                                                                          ggCAACCCCA‑T‑TTTTTTTTTTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAgg                             <  1:1342099/150‑1 (MQ=255)
                                                                                                                          gacAACTCCA‑T‑TTTTTTTTTTTTTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAgg                             <  1:30367/148‑1 (MQ=255)
                                                                                                                             aaCTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAGGGAAt                         >  1:658267/1‑151 (MQ=255)
                                                                                                                              aCTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGc                                >  1:1323150/1‑143 (MQ=255)
                                                                                                                                 ccA‑T‑TTTTTGTTTTTGTCTGAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAGGGAATGGCa                     <  2:27292/151‑1 (MQ=255)
                                                                                                                                 ccA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAGGGAATGGc                      >  1:893186/1‑150 (MQ=255)
                                                                                                                                   a‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAGGGAATGGCAAt                   >  2:1049568/1‑151 (MQ=255)
                                                                                                                                      tttttttttttTGTCTTAAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTGCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAGGGAATGGCAAt                   <  1:1049567/149‑1 (MQ=255)
                                                                                                                                       tttttGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAGGGAATGGCAATGt                 >  2:1729130/1‑151 (MQ=255)
                                                                                                                                             tATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAGGGAATGGCAATGTTTTGCg           >  2:2136598/1‑151 (MQ=255)
                                                                                                                                              aTTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAGGGa                           >  2:1593283/1‑134 (MQ=255)
                                                                                                                                                    cTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAGGGAATGGCAATGTTTTGCGAAATac     >  2:182354/1‑150 (MQ=255)
                                                                                                                                                      ggAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAGGGAATGGCAATGTTTTGCGAAATACACt  >  1:521967/1‑151 (MQ=255)
                                                                                                                                                       gAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAatat                                           >  2:954275/1‑109 (MQ=255)
                                                                                                                                                       |                                                                                                                                                      
TTTAGCTGTTTTAGTTTATCCACCACGTCATCAGGCATTCGGGTAGAAATCATGGCGGTAAAGTTAGCGACAATCTCTGTCAACTTATTCACTGCCTGTTGCTTATTACTTTCGCTCATCATGGCAACTCCA‑G‑TTTTTGTATTTGTCTGGAAAAA‑TTCACCGCCACAAACTACCACTAAAAATGTGGTTTTATATTTTTTGCCACCGTGAATATGTAATTAACAGTTCGTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAGGGAATGGCAATGTTTTGCGAAATACACT  >  DH1/677179‑677477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑