Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1899,3220TG41.3% 276.0 / 40.4 138G384G (GGT→GGG) yqeFacetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (10/71);  new base G (57/0);  total (67/71)
Fisher's exact test for biased strand distribution p-value = 8.42e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

GTCGGGTCAATGTCAATGGTGGCGCGATCGCACTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCATTTTTCCATTCTCATGACATATTC  >  DH1/899174‑899471
                                                                                                                                                     |                                                                                                                                                      
gTCGGGTCAATGTCAATGGTGGCTCGATCGCACTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGATTGGCAACGCTTTGTATCGGC‑GGGGGGCAGGgtgt                                                                                                                                                      <  1:606339/151‑1 (MQ=255)
     gtcaatgtcaatGGTGGCGCGATCGCACTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGGGTGGGAt                                                                                                                                                 >  2:1521916/1‑151 (MQ=255)
           gtcaatgGTGGCGCGATCGCACTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCAtt                                                                                                                                                <  2:131337/146‑1 (MQ=255)
             caatgGTGGCGCGATCGCACTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCAtt                                                                                                                                         <  2:863999/151‑1 (MQ=255)
              aatgGTGGCGCGATCGCACTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTg                                                                                                                                        <  1:214222/151‑1 (MQ=255)
               atgGTGGCGCGATCGCACTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGa                                                                                                                                       <  1:1061797/151‑1 (MQ=255)
                    ggCGCGATCGCACTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGt                                                                                                                                   <  1:1892853/150‑1 (MQ=255)
                    ggCGCGATCGCACTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTg                                                                                                                                  <  2:2310730/151‑1 (MQ=255)
                       gcgATCGCACTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCGGGGGGGGGGATTGTCCCTTGAACGTGACg                                                                                                                               >  1:1581030/1‑151 (MQ=255)
                       gcgATCGCACTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACg                                                                                                                               <  2:1035566/151‑1 (MQ=255)
                         gATCGCACTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGaa                                                                                                                             <  1:581204/151‑1 (MQ=255)
                         gATCGCACTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGGGTGGCATTGACCATTGAACGTGACGa                                                                                                                              >  1:1223436/1‑150 (MQ=255)
                             gCACTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGc                                                                                                                         <  2:2066577/151‑1 (MQ=255)
                             gCACTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGGGGGGGATTGACCATTTAACGGGACGaaaaa                                                                                                                          >  2:2166600/1‑147 (MQ=255)
                               aCTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGGCGGGGGGGGGGATTGACAACTGAACGTGGCGAATAggt                                                                                                                        >  1:1404388/1‑148 (MQ=255)
                               aCTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGct                                                                                                                        <  2:1404393/150‑1 (MQ=255)
                                cTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGa                                                                                                                                              <  2:34746/127‑1 (MQ=255)
                                  cGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGctct                                                                                                                      <  1:1646476/149‑1 (MQ=255)
                                      cACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCAttt                                                                                                                <  1:2320043/151‑1 (MQ=255)
                                       aCCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGtgtg                                                                                                                                                     <  2:132030/113‑1 (MQ=255)
                                             tAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGaa                                                                                                                                      <  2:1979879/122‑1 (MQ=255)
                                              aGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGAAAGGGGGCGGAATTGGCCATTGTACGTGGGGAAAAGAGCTGGTATTCCTCCttt                                                                                                        >  1:936393/1‑150 (MQ=255)
                                                 cgcTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCCGGGGGTGGCAATGACCATTTAACCTGACGAAAAACTCTGCCTTTCCTCCTTAccc                                                                                                     >  2:2224854/1‑150 (MQ=255)
                                                     tCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATa                                                                                                  <  2:423311/150‑1 (MQ=255)
                                                          ttGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCAtt                                                                                                                 <  1:1479251/130‑1 (MQ=255)
                                                             ccGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGc                                                                                         <  2:1633270/151‑1 (MQ=255)
                                                              cGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGGGTGGCATTGACCATTTAACGTGACGAATAGCTCTGCATTTTCTCCTTACCGATAAATATTTGcc                                                                                        >  2:402613/1‑151 (MQ=255)
                                                               gAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGGCAGGGGGGGGGATTGACCCTTTAAAGTGACGAAAAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCg                                                                                       >  2:1838826/1‑151 (MQ=255)
                                                                aaTCCTGGTGTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGt                                                                                      <  1:1740154/151‑1 (MQ=255)
                                                                 aTCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCCAGGGGGGGGAATGACCATTGAACGCGACGAAAAACCCTTCATTTTCTCCCTACCGATAAATATCTTCCGTc                                                                                     >  2:1879678/1‑151 (MQ=255)
                                                                  tCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGGCAGGGGGTGGCATTGACCCTTTAACGTGACGAATAGGTCTGCATTtcctcc                                                                                                           >  1:258660/1‑128 (MQ=255)
                                                                  tCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTtcctcc                                                                                                           <  2:258659/128‑1 (MQ=255)
                                                                  tCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGGGGGGCATTGACCATTGAAAGTGACGAATAACTCTGCATTTTCTCCTTACCGATAAATATCTGCCGTc                                                                                     >  1:420169/1‑150 (MQ=255)
                                                                      ggTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTa                                                                                 <  1:2217197/150‑1 (MQ=255)
                                                                      ggTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTa                                                                                 <  2:1911765/150‑1 (MQ=255)
                                                                       gTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGCGGGGGGCCAGGCGGGGGC‑GTGACCCCTGAAAGTGACCAAAAGGTCTGCCTTTTCTCCTTACCGATAAATAACTTcccgcctttat                                                                               >  1:1007089/1‑142 (MQ=255)
                                                                       gTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAAt                                                                               <  1:425600/151‑1 (MQ=255)
                                                                        tttCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGGCCGGGGGGGGGATTTACCATTTAACGTGACGAAAAACTCTGCATTTTCTCCTTACCGCTAAATATTTGCCGTCATTAATc                                                                              >  1:1185756/1‑151 (MQ=255)
                                                                         ttCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATc                                                                              <  2:1204184/150‑1 (MQ=255)
                                                                          tctcTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCCGGGTGGGGCCTTTACCACTTAACGGAACGAAAAGATCTGCCTTTTCTCCTTTCCCATAAATATATGCCGTCATTAATCTg                                                                            >  2:2632091/1‑151 (MQ=255)
                                                                          tctcTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGGGTGGGATTTACCATTTAACGTGACGAAAAGCTCTGCATTTTCTCCTTACCGATAAATATCTTCCGTCATTAATCtt                                                                            >  2:390686/1‑150 (MQ=255)
                                                                              tGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGGGGGGCATTGACCATTTAACGTGACGAAAAGCTCTGCATTTTCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTg                                                                         >  2:755583/1‑150 (MQ=255)
                                                                                gTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGaat                                                                      <  1:48447/151‑1 (MQ=255)
                                                                                gTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGACATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGaat                                                                      <  1:2632075/151‑1 (MQ=255)
                                                                                 ttCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGGGTGGCATTTAACATTTAAAGTGACGAATAACTCTGCATTTCCTCCTTTCCCCTAAATATTTGCCGTCATTAATATTTTGaat                                                                      >  1:944451/1‑150 (MQ=255)
                                                                                   cATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGGGGGGCATTGACCATTTAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGaataat                                                                   >  2:41612/1‑151 (MQ=255)
                                                                                      gAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGGCAGGGGGGGGCATTGACCATTTAACGTGACGAAAAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAatgat                                                                >  2:2468876/1‑151 (MQ=255)
                                                                                       aaaTGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGGCAGGGGGTGGGATTGGCCATTGAACGTGACGAAAAGCTCTGCATTTTCTCCTTACCGATAAATATCTGCCGTCCTTTATCTGTTtaataaatatt                                                               >  1:2361781/1‑146 (MQ=255)
                                                                                       aaaTGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAatgatg                                                               <  2:2527383/151‑1 (MQ=255)
                                                                                        aaTGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGGCAGGGGGTGGCATTGACCATTTAACGTGACGAAAAGCTCTGCATTTTCTCCTTACCGATAAATATTTGCCGTCATTAATCTGTTGaat                                                                      >  2:89054/1‑143 (MQ=255)
                                                                                        aaTGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGt                                                                                      <  2:1185800/127‑1 (MQ=255)
                                                                                           ggTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGAtggct                                                            <  2:1581035/150‑1 (MQ=255)
                                                                                           ggTGAAACGTAATGCCCGCAAAGGACTGGCAACGCGTTGTATCGGC‑GGGGGGCCGGGGGTGGCATTGACCATTTAACGTGACGAAAAACTCTGCATTTTCTCCTTACCGATAAAAATCTTCCGGCATTAATCTGTTGaataagaaccga                                                             >  2:2467627/1‑142 (MQ=255)
                                                                                           ggTGAAACGTAATGCCCGCAAAGGACTGGCAACGCGTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGaata                                                                     <  1:2467615/141‑1 (MQ=255)
                                                                                            gTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGGCAGGGGGGGGGACTGACCAATGGACGAGAAGAAAAACCTTGCATTTTCTCCTTACCGATAAAAATTTGCCGTCCTTTATCTTTTTAATAATGAaggctgg                                                          >  1:1790440/1‑151 (MQ=255)
                                                                                              gAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCa                                                        <  1:22745/151‑1 (MQ=255)
                                                                                               aaaCGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGaataa                                                                    <  1:263756/138‑1 (MQ=255)
                                                                                                aaCGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAg                                                      <  1:1838821/151‑1 (MQ=255)
                                                                                                  cGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCGGGGGGTGGCATTTACCATTTAACGGTGCGAATAGCTCTGCCTTTCCTCCTTACCCGTAAATATCTTCCGTCATTAATTTGTTGAATAATGATGGCTGGCAAGTc                                                    >  1:830801/1‑151 (MQ=255)
                                                                                                   gTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGGCCGGGGGGGGGATTGACCCTTTAACCTGACGAAAAGCTCTTCATTTTCTCCTTACCGATAAATATCTGCCGTCATTAATCTTTTGAATAATGATTGCTGGCAAGTCg                                                   >  2:1782369/1‑151 (MQ=255)
                                                                                                      aTGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGGGTGGGCTTTAACATTGAAAGTGACGAAAAGCTCTGCATTTTCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGac                                                 >  2:299596/1‑150 (MQ=255)
                                                                                                       tGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCCGGGGGGGGGATTGACCATTGAACGGGACGAAAAGCTCTGCACTTTCTCCTTACCCATAAATATCTTCCGGCATTAATCTTTTTAATAATTATGGGTGGGAAATCGacat                                               >  2:1803968/1‑151 (MQ=255)
                                                                                                        gCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGGCCGGGGGGGGGATTGACCATTGAACGCGACGAAAAGCTCTTCATTTTCTTCTTTCCCATAAAAATATTCCGGCATTAAATTTTTGTATAATTAtggcagagcggaagagggg                                              >  1:1513851/1‑136 (MQ=255)
                                                                                                        gCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGAtggc                                                             <  2:1513856/136‑1 (MQ=255)
                                                                                                          ccGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTc                                                    <  2:746509/143‑1 (MQ=255)
                                                                                                            gCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGGCAGGGGGGGGCATTGAACACTGAAAGTGACGAATAGATCTGCATTTTCTTCTTACCGATAAAAAATTGCCGTCATTAATATGTTGAATAATGATGGCTGGCAAGTCGACATA‑CAtt                                         >  1:357674/1‑151 (MQ=255)
                                                                                                              aaaGGACTGGCAACGCTTTGTATCGGC‑GGGGGGCCGGGGGTGGGATTGAGCATTTAAAGGGACGAATAACTCTGCCTTTCCTCCCTCCCCATAAATAATTGCCGCCCTTAATCTGTTGAATAATGATGGCTGGCAAGTCGGCATA‑CAtttt                                       >  2:2491470/1‑151 (MQ=255)
                                                                                                              aaaGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCCGGGGGGGGGATTTACCATTTAAAGTGACGAATAGCTCTGCCTTTTCTCCTTACCGATAAAAATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGGAAGTCGACATA‑CAtttt                                       >  2:534277/1‑151 (MQ=255)
                                                                                                              aaaGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CAtttt                                       <  1:2452942/151‑1 (MQ=255)
                                                                                                                aGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAatg                                                                  <  2:2345507/123‑1 (MQ=255)
                                                                                                                  gACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTc                                                    <  2:1566207/135‑1 (MQ=255)
                                                                                                                  gACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTgg                                   <  1:2468864/151‑1 (MQ=255)
                                                                                                                   aCTGGCAACGCTTTGTATCGGC‑GGGGGCCCGGGGGGGGGCTTGACCATTTGACGTGACGAAAAGGTCTGCCTTTCCTCCCTACCGATAAATATCTGGCGCCCTTAATCTGTTGAATAAAGATGGCTGGCAAGTCGACCTACCATTTTTTgg                                   >  2:2507868/1‑151 (MQ=255)
                                                                                                                    cTGGCAACGCTTTGTATCGGC‑GGGGGCCCGGGGGTGGCATTGACCATTTAACGTGACGAAAAGCTCTGCATTTTCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGGATAATGATGGCTGGCAAGTCGACATA‑CAtttttttggg                                 >  2:2255175/1‑145 (MQ=255)
                                                                                                                      ggCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGc                                 <  1:755582/149‑1 (MQ=255)
                                                                                                                      ggCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGc                                 <  2:1007090/149‑1 (MQ=255)
                                                                                                                      ggCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCt                                <  2:1513897/150‑1 (MQ=255)
                                                                                                                      ggCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTc                               <  1:366636/151‑1 (MQ=255)
                                                                                                                      ggCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGGGGCATTGACCATTGAAAGTGACGAAAAGCTCTGCATTTTCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTTGGCTc                               >  2:2598936/1‑151 (MQ=255)
                                                                                                                        cAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTccc                             <  2:357673/151‑1 (MQ=255)
                                                                                                                          aCGCTTTGTATCGGC‑GGGGGCCAGGGGGGGGGATTGTCCCTTTAACGTGAGGAATAACTCTGCATTTTCTCCTTACCGATTAAAATATGCCGGCATTAATATGGTTGATAATGATGGGTGGGGAGACGACATA‑AATTTTCTTGGGTccccc                           >  1:181672/1‑149 (MQ=255)
                                                                                                                          aCGCTTTGTATCGGC‑GGGGGACAGGGGGTGGGAGTTTCCCTTTAAAGCGGAGAACAGCTCCGCATTTTCTCCTTTCCCAAAAAAATCTGTCGCCATTAATTTGTTGAATAATAATTGCTGGGAAGTCGACATA‑CATTTTTTGGGCTccccc                           >  1:2283414/1‑149 (MQ=255)
                                                                                                                            gCTTTGTATCGGC‑GGGGGGCAGGGGGTGGCATTTAACATTTAACGTGACGAATAGCTCTGCATTTTCTCCCTACCGATAAATATTTGCCGGCATTAATCTGTTGAATAATGATGGGTGGCAAGTCGACATA‑AATTTTTTTGGGTCCCTCAt                         >  1:1957036/1‑151 (MQ=255)
                                                                                                                               ttGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCAtt                        <  2:2283423/149‑1 (MQ=255)
                                                                                                                                 gTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCATTTTTc                    <  2:944452/151‑1 (MQ=255)
                                                                                                                                      ggc‑ggGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCATTTTTCCATtc               >  1:2298161/1‑151 (MQ=255)
                                                                                                                                        c‑ggGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCATTTTTCCATtctc             <  2:2281744/151‑1 (MQ=255)
                                                                                                                                        c‑ggGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCATTTTTCCATtctc             <  1:2507856/151‑1 (MQ=255)
                                                                                                                                            gggCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCATTTTTCCATTCTCATg          >  1:703641/1‑151 (MQ=255)
                                                                                                                                              gCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCAt                         >  1:1573621/1‑134 (MQ=255)
                                                                                                                                              gCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCATTTTTCCATTCTCATGAc        <  2:2622236/151‑1 (MQ=255)
                                                                                                                                               ccAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCATTTTTCCATTCTCATGAc        >  2:949469/1‑150 (MQ=255)
                                                                                                                                                cAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCATTTTTCCATTCTCATGACat      >  2:2641185/1‑151 (MQ=255)
                                                                                                                                                 aGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCATTTTTCCATTCTCATGACata     <  2:1773674/151‑1 (MQ=255)
                                                                                                                                                   ggTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCATTTTTCCATTCTCATGACat      <  2:2126689/148‑1 (MQ=255)
                                                                                                                                                   ggTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCATTTTTCCATTCTCATGACat      >  1:2126681/1‑148 (MQ=255)
                                                                                                                                                    gtgtGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCATTTTTCCATTCTCATGACATATTc  >  2:567915/1‑151 (MQ=255)
                                                                                                                                                    gtgtGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCATTTTTCCATTCTCATGACATATTc  <  1:2499642/151‑1 (MQ=255)
                                                                                                                                                    gtgtGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCATTTTTCCATTCTCATGACATATTc  <  1:1782364/151‑1 (MQ=255)
                                                                                                                                                     tgtgGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCAtttt                      >  1:2564763/1‑130 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GTCGGGTCAATGTCAATGGTGGCGCGATCGCACTCGGTCACCCGATAGGCGCTTCCGGTTGCCGAATCCTGGTTTCTCTGGTTCATGAAATGGTGAAACGTAATGCCCGCAAAGGACTGGCAACGCTTTGTATCGGC‑GGGGGCCAGGGTGTGGCATTGACCATTGAACGTGACGAATAGCTCTGCATTTCCTCCTTACCGATAAATATCTGCCGTCATTAATCTGTTGAATAATGATGGCTGGCAAGTCGACATA‑CATTTTCTGGGCTCCCTCATTTTTCCATTCTCATGACATATTC  >  DH1/899174‑899471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑