Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1140,1060TG36.7% 303.3 / 30.4 139T309P (ACC→CCC) yiaK2,3‑diketo‑L‑gulonate reductase
Reads supporting (aligned to +/- strand):  ref base T (16/72);  new base G (51/0);  total (67/72)
Fisher's exact test for biased strand distribution p-value = 3.36e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

AGCGCCTTTTCAATGGCGGCGGGCAAACGGCACGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATGGCAATAAAAATTTGTGAA  >  DH1/139956‑140254
                                                                                                                                                      |                                                                                                                                                        
aGCGCCTTTTCAATGGCGGCGGGCAAACGGCACGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGgt                                                                                                                                                          <  1:1952667/151‑1 (MQ=255)
aGCGCCTTTTCAATGGCGGCGGGCAAACGGCACGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGgt                                                                                                                                                          <  2:783739/151‑1 (MQ=255)
aGCGCCTTTTCAATGGCGGCGGGCAAACGGCACGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGgt                                                                                                                                                          <  1:80039/151‑1 (MQ=255)
     cTTTTCAATGGCGGCGGGCAAACGGCACGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTTGCCCGCCGGggagga                                                                                                                                                      >  1:2136493/1‑148 (MQ=255)
       tttCAATGGCGGCGGGCAAACGGCACGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCTGCCCGCCGGGGAGG‑aaa                                                                                                                                                   >  1:2206887/1‑150 (MQ=255)
          cAATGGCGGCGGGCAAACGGCACGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCa                                                                                                                                               <  1:478300/151‑1 (MQ=255)
          cAATGGCGGCGGGCAAACGGCACGGATTGGGCTGCGCGATATCTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGCCAGCAGGGGAGT‑AAAttt                                                                                                                                                >  2:2275/1‑149 (MQ=255)
            aTGGCGGCGGGCAAACGGCACGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTTGCCAGCAGGGGAGT‑AAAATCA‑tt                                                                                                                                            >  2:1061981/1‑150 (MQ=255)
             tGGCGGCGGGCAAACGGCACGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGGAGT‑AAATTCA‑Tg‑g                                                                                                                                          >  2:2585364/1‑151 (MQ=255)
                   cggGCAAACGGCACGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGt                                                                                                                                    <  2:277567/151‑1 (MQ=255)
                     ggCAAACGGCACGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTa‑a                                                                                                                                 <  2:2533747/151‑1 (MQ=255)
                       cAAACGGCACGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCCACAGGGTAGG‑AAAATCA‑TG‑GCCGGGGA‑Agg                                                                                                                               >  2:510962/1‑150 (MQ=255)
                           cGGCACGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAAt                                                                                                                           <  1:2059251/151‑1 (MQ=255)
                            ggCACGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATg                                                                                                                          <  2:348801/151‑1 (MQ=255)
                            ggCACGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATg                                                                                                                          <  2:1014250/151‑1 (MQ=255)
                               aCGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGCCAGCAGGGGAGT‑AAATTCA‑TG‑GCCGGGGA‑AGCGAATGGcc                                                                                                                       >  2:1281374/1‑151 (MQ=255)
                                   aTTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGAt                                                                                                                   <  2:2095728/151‑1 (MQ=255)
                                     tGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGCCAGCAGGGGAGT‑AAATTCA‑TG‑GCCGGGGA‑AGCGAATGGGCTGAttt                                                                                                                 >  2:889661/1‑151 (MQ=255)
                                     tGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGAtt                                                                                                                  <  1:713228/150‑1 (MQ=255)
                                       ggCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGCCCGCCGGGGAGG‑AAAATTA‑TG‑GGCGGGGA‑AGGGGAAGGGCTGGttttt                                                                                                               >  2:1085213/1‑150 (MQ=255)
                                           gcgcGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCa                                                                                                           <  2:2199749/151‑1 (MQ=255)
                                           gcgcGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGAA‑AGCGAATGGCCTGATTTTCGTc                                                                                                            <  1:1848292/150‑1 (MQ=255)
                                             gcgATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCTGCCCGCAGGGGAGT‑AAATTCA‑TG‑GCCGGGGA‑AGCGAATGGCCTGATTTTTTTCAGc                                                                                                         >  2:1593971/1‑151 (MQ=255)
                                             gcgATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGc                                                                                                         <  2:2206896/151‑1 (MQ=255)
                                              cgATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGGAGG‑AAAATTA‑TG‑GCCGGGGA‑AGCGGATGGGCTGATTTTTTTCAGc                                                                                                         >  2:842997/1‑150 (MQ=255)
                                                atatGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGCCAGCAGGGGAGT‑AAATTCA‑TG‑GCCGGGGA‑AGCGGATGGCCTGATTTTTGTCAGCaac                                                                                                      >  2:1953552/1‑149 (MQ=255)
                                                atatGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGCCAGCAGGGGAGT‑AAAATTA‑TG‑GGCGGGGA‑AGCGAAAGGGCTGATTTTTGTCATCAcc                                                                                                      >  1:1285081/1‑150 (MQ=255)
                                                 tatGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTTGCCAGCAGGGGAAG‑AAAATCA‑TG‑GGCGGGGG‑AGGGAATGGCCTGGtttttttccgcccgc                                                                                                     >  2:1342174/1‑140 (MQ=255)
                                                   tGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGCCCGCAGGGGAGG‑AAAATCA‑TT‑GCCGGGGA‑AGCGAATGGGCTGAttttt                                                                                                               >  1:861015/1‑138 (MQ=255)
                                                       cAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCa                                                                                               <  2:2131060/151‑1 (MQ=255)
                                                         aaaaTCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACt                                                                                             <  2:698966/151‑1 (MQ=255)
                                                          aaaTCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTc                                                                                                               <  1:1527125/132‑1 (MQ=255)
                                                           aaTCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTTGCCAGCAGGGGAGG‑AAAATTA‑AG‑GCCGGGGA‑AGCGAAAGGGCTGATTTTTGTCAGCACGCTCCGCACTa                                                                                            >  2:948472/1‑150 (MQ=255)
                                                           aaTCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAg                                                                                           <  2:1285083/151‑1 (MQ=255)
                                                            aTCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGGCCGCCGGGGAGT‑AAAATTA‑TT‑GCCGGGGA‑AGGGGATGGGCTGGTTTTTGTTAGGACGccccggcccagt                                                                                          >  1:1095888/1‑140 (MQ=255)
                                                             tCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGCCAGCAGGGGAGG‑AAATTTA‑TG‑GCCGGGGA‑AGCGAATGGCCTGATTTTTGTCAGCACGCCCGGCACTAGt                                                                                          >  2:707052/1‑150 (MQ=255)
                                                              cATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTTGCCAACAGGGGAGG‑AAATTTA‑TGTGCCGGG‑A‑AGGGGATGGCCATGTTTTCTTTAGCACGGCCGGCACTCGTa                                                                                         >  1:1906895/1‑150 (MQ=255)
                                                                  aCTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTTGCCCGCAGGGTAGT‑AAATTTA‑TT‑GCCGGGGA‑AGCGAATGGCCTGATTTTTTTCAGCACGCCCGGCCCTAGTAACGt                                                                                     >  1:1303881/1‑150 (MQ=255)
                                                                   cTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGCCAGCCGGGGAGT‑AAATTTA‑TT‑GCCGGGGA‑AGCGGATGGCCTGCTTTTTGTCAGCACGCCCGGCACTAGTAACGTa                                                                                    >  1:269563/1‑150 (MQ=255)
                                                                    ttATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTTGCCCGCCGGGGAGG‑AAAATCA‑TG‑GGCGGGGA‑AGCGGAAGGCCTGATTTTTGTTCGGACGCTCGGCACTTGTaaaggaa                                                                                   >  1:449367/1‑145 (MQ=255)
                                                                      aTCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTTGCCCGCAGGGGAGG‑AAATTCA‑TG‑GCCGGGGA‑AGCGAAAGGCCTGGTTTTTGTCAGCACCCTCCGCACTAGTAAAGTAAAcc                                                                                >  1:1052556/1‑151 (MQ=255)
                                                                      aTCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGCCAGCAGGGGAGT‑AAATTCA‑TG‑GCCGGGGA‑AGCGAATGGCCTGCTTTTTGTCCGCACGCCCGGCACTAGTAACGTAATcc                                                                                >  2:1843597/1‑151 (MQ=255)
                                                                       tctcCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTCGCCAGCAGGGGAGT‑AAAATTA‑TG‑GCCGGGGG‑AGGGCATGGGCCGATTTTTGTCAGGACGCTCGGCACTAGTGAAGTAAttc                                                                                >  1:94626/1‑148 (MQ=255)
                                                                        ctcctcATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGCCAGCAGGGGAGT‑AAAATCATTG‑GCCGGG‑G‑AGCGGAAGGCCCGATTTTTGTCAGCACGCTCGGCACTAGTAAAGGAATTCat                                                                              >  1:669844/1‑151 (MQ=255)
                                                                                aaCGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGCCCGCAGGGGAGT‑AAAATCA‑TT‑GCCGGGGA‑AGGGAAAGGCCTGGTTTTTGTCAGCACGCTCGGGACTAGGAAAGTAATCCATGATGAGtt                                                                      >  1:1781887/1‑151 (MQ=255)
                                                                                 aCGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTg                                                                     <  1:2233720/151‑1 (MQ=255)
                                                                                  cGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGGCCGCAGGGGAGG‑AAAATTA‑TG‑GCCGGGGA‑AGCGAAAGGGCTGATTTTTGTCAGCACGCCCGGCACTAGTAAAGTAATCCATGATGTGTTgg                                                                    >  2:243639/1‑150 (MQ=255)
                                                                                   gCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGCCAGCAGGGGAGT‑AAATTTA‑TG‑GCCGGGGA‑AGCGAATGGCCTGATTTTTTTCAGCACGCTCGGCACTAGTAACGTAATTCATGATGCGTTGc                                                                    >  2:2163054/1‑150 (MQ=255)
                                                                                   gCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AGATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCa                                                                   <  1:2647448/151‑1 (MQ=255)
                                                                                   gCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCa                                                                   <  2:1786866/151‑1 (MQ=255)
                                                                                   gCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCa                                                                   <  1:276961/151‑1 (MQ=255)
                                                                                    ccTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGCCAGCAGGGGAGT‑AAATTTA‑TT‑GACGGGGA‑AGGGGATGGGCTGGTTTTCGTTAGCCCGCCCGGCCCTAGTAACGTAATACATTATTCGTTGc                                                                    >  1:2240910/1‑149 (MQ=255)
                                                                                      tGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGGCCGCAGGGGAGT‑AAATTTA‑TG‑GCCGGGGA‑AGCGAATGGGCTGATTTTTGTCAACACGCCCGGGACTAGTAACGGAATCCatgattggtttccgct                                                                >  2:2275625/1‑140 (MQ=255)
                                                                                       ggATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGc                                                                 <  1:1165438/149‑1 (MQ=255)
                                                                                       ggATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCt                                                                <  2:94626/150‑1 (MQ=255)
                                                                                       ggATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCt                                                                <  2:269562/150‑1 (MQ=255)
                                                                                        gATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTTGGCAGCAGGGGAGG‑AAAATTA‑TT‑GCCGGGTG‑AAGGAAAGGGCTGATTTTTTTTAGCACGCCCGGCACTATTAAAGGAAAACATAATGCGTTTCCGCttt                                                              >  1:2567698/1‑150 (MQ=255)
                                                                                        gATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGCCCGCCGGGTAGT‑AAATTCT‑TG‑GCCGGGGA‑AGAGGATGGCCCGCTTTTCGTTAGCACGCTCGGCCCCAGTAACGTAAACCAATATGCGTTTCAGCTTg                                                              >  1:147118/1‑151 (MQ=255)
                                                                                        gATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTg                                                              <  1:999905/151‑1 (MQ=255)
                                                                                         aTTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTGGGCAGCAGGGGAGT‑AAATTTA‑TG‑GGCGGGGG‑AGAGAAAGGCCTGGTTTTTGTCAGCACCCCCGGCACCAGTAAAGTAATCCATGATGCGTTGCCGCtttg                                                             >  2:1579580/1‑149 (MQ=255)
                                                                                         aTTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTgg                                                             <  1:1137042/151‑1 (MQ=255)
                                                                                           tttGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGc                                                            <  1:948471/150‑1 (MQ=255)
                                                                                                cccACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATcgcg                                                      <  2:1497113/151‑1 (MQ=255)
                                                                                                  cacaCGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTTTGCCAGCAGGGGAGT‑AAAATTA‑TG‑GGCGGGGA‑AGCGGAAGGGCTTATTTTTGTTAGCACGCCCGGGACCAATAAAGTAATACATTATGGGGTGCAGCCTGGGCTCGCggg                                                    >  1:1797509/1‑151 (MQ=255)
                                                                                                  cacaCGCTGTCATCAACAGTGATGCCGTTACGGCGGGTTTTGGGCAAAAGGGGAGT‑AAATTCA‑TA‑GCCGGGGATGGCGAAT‑GCCTGGTTTTTTTCCGCACCCTCGGGACTCAGAAAGGAAACCATCATGCGTTGCacgcctggaatccggg                                                    >  1:1983192/1‑136 (MQ=255)
                                                                                                   acacGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGt                                                   <  1:617975/151‑1 (MQ=255)
                                                                                                    cacGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTg                                                  <  2:669845/151‑1 (MQ=255)
                                                                                                           tcatcaACAGTGATGCCGTTACGGCGGTTTTTGGGCCGCAGGGGAGT‑AAATTCA‑TG‑GCCGGGGA‑AGCGAATGGGCTGATTTTTTTCAGGACGCTCGGCACTAGGAAAGGAAAACATGATGCGTTTCAGCTTGGCATCGCGgggggggacgg                                           >  1:93770/1‑146 (MQ=255)
                                                                                                           tcatcaACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGGAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGGCTGATTTTCCTCAGCACCCTCCGCACTAGTAACATAATCCATGATGCGTTGCAGCTTGGCATCGCGGGGGGGGCCGt                                           >  2:312875/1‑151 (MQ=255)
                                                                                                            catcaACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGGAGT‑AAATTTA‑TG‑GCCGGGGA‑AGCGAAAGGGCTGATTTTTGTCAGCACCCTCGGCACCAGTAAAGTAATCCATGATGCGTTGCCGCTTGGCATCGCGGGGGGGGCCggc                                          >  1:832596/1‑149 (MQ=255)
                                                                                                             atcaACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCg                                         <  2:89119/151‑1 (MQ=255)
                                                                                                                aaCAGTGATGCCGTTACGGCGGTTTTCGGGCAGCCGGGGAGTAAAATTAA‑TG‑GCCGGG‑A‑AGCGAATGGGCTGGTTTTTGTCAGCACCCTCCGCACTAGTAACGTAAACCATGATGCGTTTCAGCTTGGCATCGCGGGGGGGGCCGCCGAt                                       >  1:2151369/1‑150 (MQ=255)
                                                                                                                   aGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGc                                   <  1:1596800/151‑1 (MQ=255)
                                                                                                                    gTGATGCCGTTACGGCGGTTTTTGGCCAGCAGGGGAGG‑AAAATTA‑TT‑GGCGGGGA‑AGGGGATGGGCTGATTTTTGTCAGCAACCGCGGCACTAGTAAAGGAAATCATGATGGGTTTCAGCTTGGCATCGCGGGGGGGGACGGCGATaaagt                                  >  1:844784/1‑148 (MQ=255)
                                                                                                                       aTGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGt                               <  1:2163046/151‑1 (MQ=255)
                                                                                                                         gCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTcc                             <  1:707051/151‑1 (MQ=255)
                                                                                                                            gTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACt                          <  1:243640/151‑1 (MQ=255)
                                                                                                                                cggcggTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCa                       >  1:2387058/1‑150 (MQ=255)
                                                                                                                                 ggcggTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAAt                     <  1:1061980/151‑1 (MQ=255)
                                                                                                                                 ggcggTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAAt                     <  1:1579575/151‑1 (MQ=255)
                                                                                                                                 ggcggTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAAt                     >  1:2203251/1‑151 (MQ=255)
                                                                                                                                 ggcggTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAAt                     >  1:1270123/1‑151 (MQ=255)
                                                                                                                                 ggcggTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑CG‑GCCGGGCA‑AACGGATGGCCTGATTTTCGTCAGCACGCTCGGCACTCGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGCCCGTCGATAAGCTTGTCCACTTCAAt                     >  1:2005092/1‑151 (MQ=255)
                                                                                                                                  gcggTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTc                        >  2:1108286/1‑147 (MQ=255)
                                                                                                                                  gcggTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTc                        <  1:1108285/147‑1 (MQ=255)
                                                                                                                                  gcggTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATg                    <  2:2387068/151‑1 (MQ=255)
                                                                                                                                  gcggTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATg                    <  1:2275616/151‑1 (MQ=255)
                                                                                                                                  gcggTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATg                    >  2:1843745/1‑151 (MQ=255)
                                                                                                                                    ggTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATGGc                  >  2:1223292/1‑151 (MQ=255)
                                                                                                                                    ggTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATGGc                  <  2:1797514/151‑1 (MQ=255)
                                                                                                                                       tttCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATGGCAAt               >  2:349047/1‑151 (MQ=255)
                                                                                                                                         tCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATGGCAATa              >  1:1019893/1‑150 (MQ=255)
                                                                                                                                         tCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATGGCAATa              <  2:1516836/150‑1 (MQ=255)
                                                                                                                                           ggCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATGGCAATaaaa           <  1:2308261/151‑1 (MQ=255)
                                                                                                                                            gCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATGGCAATaaaaa          <  2:1906900/151‑1 (MQ=255)
                                                                                                                                                  agGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATGGCAATAAAAATTtgtg    >  1:1248868/1‑151 (MQ=255)
                                                                                                                                                   gggTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATGGCAATAAAAATTtgtg    >  2:354443/1‑150 (MQ=255)
                                                                                                                                                     gtagt‑aAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATGGCa                 >  2:2535621/1‑135 (MQ=255)
                                                                                                                                                     gtagt‑aAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATGGCa                 <  1:2535608/135‑1 (MQ=255)
                                                                                                                                                     gtagt‑aAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATGGCAATAAAAATTTGTGaa  >  1:2090866/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                        
AGCGCCTTTTCAATGGCGGCGGGCAAACGGCACGGATTGGGCTGCGCGATATGTCCAAAAATCATGACTTATCTCCTCATAACGCCTGGATTTTGGCCCACACGCTGTCATCAACAGTGATGCCGTTACGGCGGTTTTCGGCCAGCAGGGTAGT‑AAATTCA‑TG‑GCCGGGTA‑AGCGAATGGCCTGATTTTCGTCAGCACGCTCGGCACTAGTAACGTAATCCATGATGCGTTGCAGCTTGGCATCGCGGGTGGGACCGTCGATAAGCTTGTCCACTTCAATGGCAATAAAAATTTGTGAA  >  DH1/139956‑140254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑