Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,628,4350GT37.7% 256.0 / 79.5 146V37F (GTT→TTT) lysPlysine‑specific permease
Reads supporting (aligned to +/- strand):  ref base G (13/78);  new base T (55/0);  total (68/78)
Fisher's exact test for biased strand distribution p-value = 4.58e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

GCTACAATCGCGCCTCATTTTTAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGTTTCCGGTTCGTTTGCCACTTACGGTC  >  DH1/1628285‑1628585
                                                                                                                                                      |                                                                                                                                                       
gCTACAATCGCGCCTCATTTTTAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTttg                                                                                                                                                         <  2:2019432/151‑1 (MQ=255)
      aTCGCGCCTCATTTTTAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCct                                                                                                                                                   <  2:290261/151‑1 (MQ=255)
        cgcgCCTCATTTTTAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCctct                                                                                                                                                 <  2:1127343/151‑1 (MQ=255)
         gcgcCTCATTTTTAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCATCGGTACAGGGCTTTTTTTTGTctctt                                                                                                                                                >  1:756361/1‑150 (MQ=255)
         gcgcCTCATTTTTAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGGACAGGGCTTTTTTTTTGctctt                                                                                                                                                >  1:1841035/1‑150 (MQ=255)
           gcCTCATTTTTAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCCTCGGGACAGGTCTTTttgatcggaagagg                                                                                                                                              >  1:1384256/1‑140 (MQ=255)
           gcCTCATTTTTAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGgc                                                                                                                                              <  2:477813/151‑1 (MQ=255)
           gcCTCATTTTTAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGGACAGGGCTTTTTTTTGCCTCTTgc                                                                                                                                              >  1:1403051/1‑151 (MQ=255)
             cTCATTTTTAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCATCGGGACAGGTCTTTTTTTTTCCTCTGgcg‑c                                                                                                                                           >  1:1718659/1‑151 (MQ=255)
                   tttAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCATCGGTACAGGTCTTTTTTTTGCCTCTGGCG‑CAACgct                                                                                                                                     >  1:719507/1‑149 (MQ=255)
                   tttAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCATCGGTAAAGGGCTTTTTTTTTCCTCCTGCG‑CCACGAt                                                                                                                                     >  1:1150352/1‑151 (MQ=255)
                   tttAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCCTCTGTACAGGGCTTTTTTTTGtctccgatcgggag                                                                                                                                        >  1:2170999/1‑139 (MQ=255)
                   tttAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCctc                                                                                                                                                  <  2:2171007/139‑1 (MQ=255)
                   tttAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGGCTTTTTTTTGCCTCTGGCG‑CAACGAt                                                                                                                                     >  2:1401647/1‑151 (MQ=255)
                    ttAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGAt                                                                                                                                     <  1:1519549/150‑1 (MQ=255)
                     tAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCCTTGGGACAGGGCTTTTTTTTTCCTCTCGCG‑CCACGAttt                                                                                                                                   >  1:909761/1‑151 (MQ=255)
                     tAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCATCCGGACCGGGCTTTTTGGTGCCTCTGGCG‑CCACGGttt                                                                                                                                   >  2:1263027/1‑151 (MQ=255)
                     tAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGAtt                                                                                                                                    <  1:1013163/150‑1 (MQ=255)
                       aGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGAGCAGGTCTTTTTTTTGCCTCTTGCG‑CAACGCttttt                                                                                                                                 >  2:226604/1‑149 (MQ=255)
                         aTGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGGACAGGGCTTTTTTTTTCCTCTGGCG‑CAACGagatcggg                                                                                                                               >  2:323075/1‑144 (MQ=255)
                          tGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGGACAGGGCTTTTTTTTGCCTCTTGCG‑CAACGATTtttc                                                                                                                                >  2:173529/1‑149 (MQ=255)
                              tAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTTTTTCCTCTGGCG‑CAACGATTTCTccggc                                                                                                                            >  2:1451593/1‑149 (MQ=255)
                               aGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTcaggcag                                                                                                                          <  2:2165224/150‑1 (MQ=255)
                                 cATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTc                                                                                                                       <  1:2472162/151‑1 (MQ=255)
                                   tttttGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGAttt                                                                                                                                   <  2:609973/137‑1 (MQ=255)
                                   tttttGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGGCTTTTTGTTGCCTCTGGCG‑CAACGAttttgatcgggagagcg                                                                                                                     >  1:609972/1‑137 (MQ=255)
                                    ttttGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCTTCTGCGCAGGGCTTTTTTTTGGCTCTGGGG‑CCACGAGTTCTcagggaggggcgg                                                                                                                    >  1:79613/1‑146 (MQ=255)
                                    ttttGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCcg                                                                                                                     <  2:1841040/150‑1 (MQ=255)
                                     tttGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGGACAGGTCTTTTTTTTTCCTCTGGCG‑CAACGATTTTTCAGGCAGGGCcggg                                                                                                                   >  2:1261917/1‑151 (MQ=255)
                                      ttGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCcggg                                                                                                                   <  2:2556096/150‑1 (MQ=255)
                                        gTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCCTCGGTACAGGGCTTTTTTTTGTCTCTGGCG‑CCAAGATTTTTCCGGCAGGTCcgggggg                                                                                                                >  2:1481548/1‑151 (MQ=255)
                                          aTGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCCTCGGGACAGGGCTTTTTTTTGGCTTTTGCG‑CCACGGTTTTTCACGCAGGGCCGGGCgggg                                                                                                              >  1:70713/1‑151 (MQ=255)
                                          aTGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCcgggcggg                                                                                                               <  2:379043/150‑1 (MQ=255)
                                           tGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCCTCGGTACAGGGCTTTTTTTTTCCTCTGGCG‑CCACGATTTCTCAGGCAGGTCCggggggggg                                                                                                             >  2:1706286/1‑150 (MQ=255)
                                           tGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCCTCGGGACAGGGCTTTTTTTTGCCTCTGGCG‑CAACGATTTTTCCTGCAGGTCCGGGCggggg                                                                                                             >  2:589198/1‑150 (MQ=255)
                                            ggTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCa                                                                                                            <  2:750838/151‑1 (MQ=255)
                                             gTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCATCGGGGCAGGGCTTTTTTTTGTCTCCTGGG‑CAACGATTTTTCCGGGAGGGCCGGGCGGGGGAt                                                                                                           >  2:1864110/1‑151 (MQ=255)
                                             gTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCAt                                                                                                           <  2:756362/151‑1 (MQ=255)
                                               ttCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCAtt                                                                                                          <  1:387702/150‑1 (MQ=255)
                                                tCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCCTCGGTACAGGTCTTTTTTTTGCCTCTGGCG‑CAACGAATTTTCCGGCAGGTCCGGGCGGGGCATTgt                                                                                                        >  2:427413/1‑150 (MQ=255)
                                                  cGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCATCGGGACAGGGCTTTTTTTTTCCTCTGGCG‑CAACGATTTTTCAGGCAGGTCCGGGCGGGGGGTTGctc                                                                                                      >  1:584895/1‑151 (MQ=255)
                                                  cGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGctc                                                                                                      <  1:1369071/151‑1 (MQ=255)
                                                   gAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCATCGGGACAGGGCTTTTTTTTGTCTCTGGGG‑CAACGATTTTTCAGGCAGGTCCGGGCGGGGGGTTGctct                                                                                                     >  1:383858/1‑151 (MQ=255)
                                                    aaaCTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCCTCGGCAAAAGGGTTTTTTTTGGCCCTGGGG‑GAACGATTTCTTAGGCAGGTGCGGGCGGGGGATTGcttcc                                                                                                    >  1:2400667/1‑148 (MQ=255)
                                                       cTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCCGTACCGGGCTTTTTTTTGCCTCTCGCG‑CCACGATTTCTCCGGCCGGTCCGGGCGGGGccgtgttttcgtt                                                                                                 >  1:1956614/1‑139 (MQ=255)
                                                         taaaCCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCCTCGGGACAGGGCTTTTTTTTGGCTCTGGCG‑GAACGATTTTTTAGGCAGGGCCGGGCGGGGGATTTTTCTCGtatt                                                                                               >  1:92862/2‑150 (MQ=255)
                                                         aaaaCCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGtata                                                                                               <  2:406192/151‑1 (MQ=255)
                                                         aaaaCCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGtat                                                                                                <  2:70713/150‑1 (MQ=255)
                                                         aaaaCCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGATCCCTCGGGTCAGGTTTTTTTTTTGTTTCTGGCG‑CAACCAGTTTTAAGGCCGGGGCGGGCGGGGCAGTtctccctcga                                                                                                >  1:1193083/1‑144 (MQ=255)
                                                            aCCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCCTCGGGACAGGGCTTTTTTTTTGCCCTTGCG‑CAACGATTTTTCAGGCAGGGCCCGGGGGGGGCTTGCTCTCGTATTTGc                                                                                            >  1:2603283/1‑151 (MQ=255)
                                                             ccACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCt                                                                                           <  1:427414/151‑1 (MQ=255)
                                                              cacaGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTg                                                                                          <  2:2425226/151‑1 (MQ=255)
                                                              cacaGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGGACAGGTCTTTTTTTTTTCTCTTGCG‑CCACGATTTCTCAGGCAGGGCCGGGCGGGGGCTTTCTCTTGTTTATTCtt                                                                                          >  1:433431/1‑150 (MQ=255)
                                                               acaGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGa                                                                                         <  1:1594577/151‑1 (MQ=255)
                                                                caGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCCACTGTACAGGGCTTTTTTTTTGCTCCGGCG‑CCACCATTTCTCAGGCAGGTCCGGGGGGGGCCTTGCTCTCGTATATGCTGAt                                                                                        >  2:1578674/1‑151 (MQ=255)
                                                                caGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGAt                                                                                        <  2:383857/151‑1 (MQ=255)
                                                                 aGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCATCGGTACAGGTCTTTTTTTTGCCTCTTGCG‑CAACGAATTTTCAGGCAGGTCCGGGGGGGGGATTTCTCTCGTATATTCTGAtt                                                                                       >  2:2423797/1‑151 (MQ=255)
                                                                 aGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCCTCGGTACAGGGCTTTTTGTTGCCTCTGGCG‑CAACGATTTTTTAGGCAGGGCCGGGGGGGGCCTTTCTCTCGTTTATGCTGAtt                                                                                       >  1:325409/1‑151 (MQ=255)
                                                                   aaGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCATTGGGACAGGGCTTTTTTTTGCCCCTTGGG‑CCACGATTTTTTAGGGAGGTCCCGGGGGGGGCTTTTTCTCGTTTATGCTGGTTgg                                                                                     >  1:1657950/1‑151 (MQ=255)
                                                                    aGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGGTTTCTCGGCACCGGTCTTTTTTTTTGCTCCCGCG‑CAACGATTTCTCAGGCGGGGCCGGGTGGGGCCTTGCTCCCGTATATGCtcagttgg                                                                                    >  2:1924714/1‑144 (MQ=255)
                                                                        ccGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGa                                                                                <  2:1641382/151‑1 (MQ=255)
                                                                         cGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTgg                                                                                     <  2:1066646/145‑1 (MQ=255)
                                                                          gggCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCATCGGGGGAGGGCTTTTTTTTGGCTCCGGCG‑CAAAGCTTTCTCAGGCAGGGCCGGGGGGGGGCTTGCTCTCTTTTATTCTGAtttggcttagg                                                                              >  1:618992/1‑142 (MQ=255)
                                                                             cTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTgg                                                                                     <  1:1889292/141‑1 (MQ=255)
                                                                               tACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGtt                                                                           <  2:2370083/149‑1 (MQ=255)
                                                                                  gCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCCTCGGTTCAGGGCTTTTTTTTGCCGCTGGCG‑GAACGAGTTTTTAGGCAGGGCCGGGGGGGGGGTTGGTTTCGTATATGGTGATTGGGCTggggggtttctt                                                                      >  2:1302520/1‑140 (MQ=255)
                                                                                    cGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTcc                                                                    <  2:92862/151‑1 (MQ=255)
                                                                                     gTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCt                                                                   <  1:173529/151‑1 (MQ=255)
                                                                                      tGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCATCGGTACAGGGCTTTTTGTTGCCCCTGGCG‑CAACGATTTTTCCGGGAGGTCCGGGCGGGGGATTGCTTTCGTATATGCTGATTTGCCTGATGGTTttctttctt                                                                  >  1:1631074/1‑150 (MQ=255)
                                                                                          ttAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCATCGGGACAGGGCTTTTTTTTTCCTCTTGCG‑CAACGATTTTTCAGGCAGGGCCGGGCGGGGGATTTCTCTCCTATATTCTGATTTGGCTGATGGTTTTCTTCCttatt                                                               >  2:169811/1‑149 (MQ=255)
                                                                                            aaaGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGAc                                                             <  2:2603299/150‑1 (MQ=255)
                                                                                             aaGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTgg                                                                                     <  2:681875/125‑1 (MQ=255)
                                                                                             aaGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGGACAGGGCTTTTTTTTTCCTCTGGCG‑CCACGATTTTTCTGGCCGGTCCCGGGGGGGGGTTGTTCTCGTTTTTGTTGGTTTGGCTGGTGGGTTTCTTTCTTGTGAcc                                                            >  1:1572753/1‑150 (MQ=255)
                                                                                              aGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTTCCTCGGGACAGGGCTTTTTTTTTCCTCTGGCG‑CCACGATTTTTCCGGCAGGGCCCGGCGGGGCCTTGCTCTCGTATATGCTGATTTGGCTGATGGGTTTCTTCCTGATTAcc                                                            >  2:2226657/1‑149 (MQ=255)
                                                                                                 cgcgTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGtct                                                       <  2:1572758/151‑1 (MQ=255)
                                                                                                   cgTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCg                                                     <  2:162088/151‑1 (MQ=255)
                                                                                                       aCCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGa                                                 <  1:1451588/151‑1 (MQ=255)
                                                                                                        ccTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCt                                                                   <  1:2399370/132‑1 (MQ=255)
                                                                                                        ccTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGGCTTTTTTTTGCCTCTTGCG‑CAACGATTTTTCAGGCAGGGCCGGGGGGGGCATTGCTCTCGTATATGCTGATTTGCCTGATGGTTTACTTCCt                                                                   >  2:2399381/1‑132 (MQ=255)
                                                                                                         cTGACGATGATTGCCATTGGCGGTTTCCTCGGGACAGGTCTTTTTTTTGCCTCTGGCGCCAAGGATTTTTC‑GGGAGGGCCCGGCGGGGGACTTCTCCTGTTTTTGGTGATTGGCCTGGTGGTTTACTTTCTTTTGAGCCGTCTCGGGGaa                                                >  1:229116/1‑150 (MQ=255)
                                                                                                         cTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTg                                                  <  1:430477/148‑1 (MQ=255)
                                                                                                           gACGATGATTGCCATTGGCGGTTTCCTCGGGACAGGTCTTTTTTTTGCCCCTGGGG‑CAACGATTTTTCAGGGGGGGCCGGGGGGGGGGTTTTTTTCGTTTATTTTGTTTTGCCTGTGGGTTTTTTTTCTGTTGACCaaaaacggaaaagc                                              >  2:1367919/1‑137 (MQ=255)
                                                                                                              gatgatTGCCATTGGCGGTTCCATCGGTACAGGTCTGTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCt                                          <  1:2173986/151‑1 (MQ=255)
                                                                                                                        aTTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGc                                <  2:1175652/151‑1 (MQ=255)
                                                                                                                           ggCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCg                              <  2:429700/150‑1 (MQ=255)
                                                                                                                              ggTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGttt                          <  1:1302518/151‑1 (MQ=255)
                                                                                                                              ggTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGttt                          >  1:1060868/1‑151 (MQ=255)
                                                                                                                                  ccATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGTTTCCgg                      <  2:79613/151‑1 (MQ=255)
                                                                                                                                      cGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGTTTCCGGTTCg                  >  2:1609753/1‑151 (MQ=255)
                                                                                                                                        gTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGTTTCCGGTTCGtt                <  1:169811/151‑1 (MQ=255)
                                                                                                                                          aCAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGTTTCCGGTTCGTTTg              <  1:424794/151‑1 (MQ=255)
                                                                                                                                           cAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGTTTCCGGtt                    <  1:1305690/144‑1 (MQ=255)
                                                                                                                                           cAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGTTTCCGGTTCGTTTGc             <  2:909762/151‑1 (MQ=255)
                                                                                                                                              gTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGTTTCCGGTTCGTTTGCCAc          <  1:1924709/151‑1 (MQ=255)
                                                                                                                                                 tttttGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGTTTCCGGTTCGTTTGc             >  1:544960/1‑145 (MQ=255)
                                                                                                                                                 tttttGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGTTTCCGGTTCGTTTGc             <  2:544961/145‑1 (MQ=255)
                                                                                                                                                  ttttGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGTTTCCGGTTCGTTTGCCACTTa       <  1:226605/150‑1 (MQ=255)
                                                                                                                                                    ttgttgCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGTTTCCGGTTCGTTTGCCACTTACg     <  1:1609748/150‑1 (MQ=255)
                                                                                                                                                      gttgCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGTTTCCGGTTCGTTTGCCACTTACGGt   <  2:129106/150‑1 (MQ=255)
                                                                                                                                                      gttgCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGTTTCCGGTTCGTTTGCCACTTACGGTc  <  2:1657955/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
GCTACAATCGCGCCTCATTTTTAAGATGGATAGCATTTTTGTATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCATTGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCG‑CAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCTCGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGTTTCCGGTTCGTTTGCCACTTACGGTC  >  DH1/1628285‑1628585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑