Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,255,6620CT36.7% 175.3 / 90.7 128intergenic (+144/+75)dgcZ/yneMdiguanylate cyclase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (8/73);  new base T (47/0);  total (55/73)
Fisher's exact test for biased strand distribution p-value = 4.73e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

AGTTTAAGCTCCGTTTAACATTCATTGAGAAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGCCAGAAAACCAGAAAATAAAATTATTCCCAGTACGGCCATAAA  >  DH1/2255512‑2255811
                                                                                                                                                      |                                                                                                                                                                  
 gTTTAAGCTCCGTTTAACATTCATTGAGAAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCg                                                                                                                                                                   <  1:1726068/151‑1 (MQ=255)
     ctgCTCCGTTTAACATTCATTGAGAAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAACCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGcc                                                                                                                                                                 <  2:36652/147‑1 (MQ=255)
     aaGCTCCGTTTAACATTCATTGAGAAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑g                                                                                                                                                               <  2:1293131/150‑1 (MQ=255)
           cGTTTAACATTCATTGAGAAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GT‑CCT‑aa                                                                                                                                                       >  2:1731867/1‑150 (MQ=255)
            gTTTAACATTCATTGAGAAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑aaaa                                                                                                                                                     <  2:1347406/151‑1 (MQ=255)
              ttAACATTCATTGAGAAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑aa                                                                                                                                                       <  1:1404301/147‑1 (MQ=255)
               tAACATTCATTGAGAAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GT‑CAT‑AAAAAt                                                                                                                                                   >  1:1253621/1‑150 (MQ=255)
                 aCATTCATTGAGAAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑Ag                                                                                                                                               <  2:580951/151‑1 (MQ=255)
                   attcattGAGAAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGc                                                                                                                                             <  2:351174/151‑1 (MQ=255)
                     tcattGAGAAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGcc                                                                                                                                            <  2:1253623/150‑1 (MQ=255)
                      cattGAGAAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGccc                                                                                                                                           <  2:1132135/150‑1 (MQ=255)
                         tGAGAAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑tt                                                                                                                                       <  2:2592494/150‑1 (MQ=255)
                         tGAGAAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑tt                                                                                                                                       <  2:1762910/150‑1 (MQ=255)
                         tGAGAAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑tt                                                                                                                                       <  2:550041/150‑1 (MQ=255)
                          gagaAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GC‑CAT‑AAAAAAA‑AAGcccccttt                                                                                                                                      >  2:1446060/1‑147 (MQ=255)
                          gagaAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTg                                                                                                                                      <  2:2508467/150‑1 (MQ=255)
                          gagaAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTg                                                                                                                                      <  2:1404774/150‑1 (MQ=255)
                             aaaaCTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GC‑CAT‑aaaaaaaa                                                                                                                                                 >  2:1522210/1‑135 (MQ=255)
                             aaaaCTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTGTC‑GG‑CCT‑AAAAAAA‑AAGGCCC‑TTTTac                                                                                                                                   >  1:1546604/1‑150 (MQ=255)
                             aaaaCTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑a                                                                                                                                                <  1:1522205/138‑1 (MQ=255)
                             aaaaCTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTCTTTTTTTTCC‑CG‑CCT‑AAAAAAC‑ACGCCCC‑CTGtttc                                                                                                                                  >  1:1904966/1‑148 (MQ=255)
                                   gATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCT‑GG‑CCT‑AAAAAAC‑AGGCCCC‑CTTGACA‑Caaatc                                                                                                                           >  1:1088645/1‑148 (MQ=255)
                                    aTGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GT‑CAT‑TAAAAAA‑AGGCCCC‑CTTTTCA‑CAACTG‑t                                                                                                                         >  2:972304/1‑151 (MQ=255)
                                    aTGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑t                                                                                                                         <  2:125585/151‑1 (MQ=255)
                                         aCTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GT‑CAT‑AAAAAAA‑AAGCCCC‑CTTTTCA‑CAAATG‑T‑aaaa                                                                                                                    >  1:469513/1‑148 (MQ=255)
                                         aCTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GG‑CCT‑AAAAAAA‑AGGCCCC‑CTTTACA‑AAAATG‑T‑aaaa                                                                                                                    >  2:858911/1‑148 (MQ=255)
                                           tgtgTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GT‑CAT‑AAAAAAA‑AGGGCCC‑CTGTtca                                                                                                                                  >  2:2109142/1‑137 (MQ=255)
                                           tgtgTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTaca                                                                                                                                  <  1:2109134/137‑1 (MQ=255)
                                              gtCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GG‑CCT‑AAAAAAA‑AAGCCCC‑CTTTACC‑CAAATT‑T‑AAA‑AA‑agggg                                                                                                            >  2:2559669/1‑150 (MQ=255)
                                                 aaCGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑gg                                                                                                        <  2:1546609/151‑1 (MQ=255)
                                                     aTTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑CT‑CAT‑AAAAAAA‑AGGCCCC‑TTTTTCA‑CAACTC‑T‑AAC‑AA‑AGGGGC‑GGtttg                                                                                                    >  1:251728/1‑149 (MQ=255)
                                                        cGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GT‑CCT‑AAAAAACAAGGCCCC‑TT‑TACA‑CAACTG‑T‑AACAAA‑GGGGCG‑GGTTgggg                                                                                                  >  1:895111/1‑150 (MQ=255)
                                                          ttATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTTC‑GTCCTT‑AAAAAAA‑AGGCCCCTTTTTACA‑AAACT‑‑T‑AAA‑AA‑GGGGCG‑GGttggtggg                                                                                                >  1:2149000/1‑145 (MQ=255)
                                                          ttATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACCGGCGGTTTTTTTTTTCC‑GT‑TCT‑AAAAAAA‑AAGGCCC‑TTTTACT‑CACCAG‑TAAAA‑AA‑GGGGCG‑GGTTgggg                                                                                                  >  1:2631197/1‑148 (MQ=255)
                                                            aTCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTGCC‑CT‑CAT‑AAAAAAA‑AAGCCCC‑CTGTTCACCAATGG‑T‑AAA‑AA‑GGGCCC‑GGTT‑GGTGgggg                                                                                             >  2:1394580/1‑151 (MQ=255)
                                                            aTCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAgg                                                                                              <  1:972303/150‑1 (MQ=255)
                                                                gTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTTC‑GG‑CAT‑AAAAAAA‑AAGACCC‑CTGTTCACCAACTC‑A‑AAA‑AA‑GGGG‑C‑GGGTAGGGGAGGGGttt                                                                                         >  1:2365472/1‑150 (MQ=255)
                                                                  gCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGGTTTTTTTTTCC‑GT‑CAT‑AAAAAAT‑AAGACCC‑TTTTTCA‑CAACTG‑T‑AAA‑AA‑AGGGGC‑GGGTTGGTGAGGGAGTAtt                                                                                       >  1:2335119/1‑150 (MQ=255)
                                                                    aGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑CT‑CCT‑AAAAAAA‑AAGCCCC‑CTTTTCA‑CAAAAGTT‑AAA‑AA‑GGGGCG‑GGTTGGGGAGGGGAtttttt                                                                                      >  1:1986721/1‑147 (MQ=255)
                                                                     ggAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTcc                                                                                    <  2:626247/151‑1 (MQ=255)
                                                                      gAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑TT‑CAT‑AAAAAAC‑AGGCCCC‑TTGTACG‑CCACTT‑T‑AAC‑CA‑CGGGGC‑GGGTATGTGAggggatgtgttc                                                                                    >  1:2405260/1‑141 (MQ=255)
                                                                       aaaaTGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGt                                                                                  <  2:1904971/151‑1 (MQ=255)
                                                                        aaaTGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGtt                                                                                 <  2:2149008/151‑1 (MQ=255)
                                                                          aTGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCa                                                                               <  2:2631213/151‑1 (MQ=255)
                                                                            gCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCAt                                                                              <  2:2405271/150‑1 (MQ=255)
                                                                             ccTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCCGGC‑CTT‑AAAAACA‑AGGCCCC‑TT‑TACA‑CCAACG‑T‑AAC‑AA‑AGGGGC‑GGGTAGGGGGGGGGGTATTTCccttccttt                                                                            >  2:2289632/1‑150 (MQ=255)
                                                                             ccTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTa                                                                            <  1:858910/151‑1 (MQ=255)
                                                                               tGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑g                                                                                                         <  1:575536/120‑1 (MQ=255)
                                                                                gTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑CT‑CAT‑AAAAATA‑AAGCCCC‑CTTTTTA‑CAAAAG‑T‑AAA‑AA‑AGGGGC‑GGGTTGGTGAGGGGGTATTTCCGtttctttttt                                                                         >  2:477160/1‑143 (MQ=255)
                                                                                gTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGt                                                                          <  2:837065/150‑1 (MQ=255)
                                                                                     cGTAAAAGCAAAACGCAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GC‑CCT‑AAAAAAA‑AAGCCCC‑CTTTAAA‑CAAATG‑T‑AAA‑AA‑AGGGCCGGGTTAGGGGGGGGGTTTTTTCCtttattttttattcc                                                                     >  1:634434/1‑139 (MQ=255)
                                                                                     cGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GG‑CCT‑AAAATAT‑AAGCCCC‑TTGTACA‑CCACTG‑G‑AAC‑AACAGGGCG‑GGTTTGG‑GAGGGGTTATCTTCGCTCTTTTATCAT‑ccc                                                                   >  1:2602652/1‑151 (MQ=255)
                                                                                     cGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑CT‑CAT‑TAAAAAC‑AAGCCCC‑TTTTTCA‑CAAATG‑T‑AAA‑AA‑AGGGGC‑GGGTAGGGGAGGGGTTATTTCCCTTCCTTATTTAT‑ccc                                                                   >  2:1087090/1‑151 (MQ=255)
                                                                                     cGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑ccc                                                                   <  1:2289623/151‑1 (MQ=255)
                                                                                      gTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAgg                                                                                                   <  2:163614/119‑1 (MQ=255)
                                                                                             cAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTa                                                                            <  1:2231098/135‑1 (MQ=255)
                                                                                                 acacAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTGGC‑GG‑CCT‑AAAAAAC‑AAGCCCC‑CTCTACA‑CAACCT‑T‑AAA‑AA‑AGGGAC‑GGGTAGGTGGGGGGATATTTTCCTTCCTTTGTTCT‑CCCAttttttggtgg                                                       >  2:915977/1‑143 (MQ=255)
                                                                                                 acacAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGa                                                       <  1:573903/151‑1 (MQ=255)
                                                                                                  cacaAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGaa                                                      <  2:469513/151‑1 (MQ=255)
                                                                                                   acaAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GG‑CCTAAAAAAAA‑AGACCCC‑TCTTCCC‑CAAC‑C‑T‑AAC‑AA‑GGGGGC‑GGGTTGGGGAGGGGTTTTTTCCCTTTATTTGTCAT‑CCCCCTTGTgggttta                                                      >  2:16565/1‑145 (MQ=255)
                                                                                                    caAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTGCC‑GT‑CAT‑AAAAAAC‑AAGCCCC‑CTTTACA‑CCAATG‑T‑AAA‑AA‑AGGGGC‑GGGTTGGTGAGGGGTTATTTCCGTTCATTTGTCAT‑TCCCTTTTTTGCTGaaa                                                     >  2:2662672/1‑150 (MQ=255)
                                                                                                     aaaTCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGt                                                                          <  1:2075067/129‑1 (MQ=255)
                                                                                                      aaTCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATAc                                                  <  2:634435/151‑1 (MQ=255)
                                                                                                       aTCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGGTTTTTTTTTCC‑CT‑CCTCAAAAAAA‑AGACCCC‑TT‑TACA‑CAAATG‑T‑AAC‑AA‑AGGGGC‑GGGTTGGGGGGGGGTTAACTCCTTTCCTTTGTCCT‑CCCATTTTTTGGTGaaaaaa                                                  >  1:466762/1‑147 (MQ=255)
                                                                                                         cTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GG‑CCT‑AAAAAAA‑AAGCCCC‑CTTTTCA‑CCCCAG‑TAAAA‑AA‑AGGGCC‑GGTT‑GGGGGGGGGTTATCTCCCTTCATTAGTCATCCCCATTTTTGG‑TGTAAAAcgc‑g                                              >  2:2641830/1‑151 (MQ=255)
                                                                                                          tATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATAc                                                  <  1:1435423/147‑1 (MQ=255)
                                                                                                           aTCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GT‑CCT‑AAAAAAC‑AGGGCCC‑CTTTATA‑CAAAAG‑TAAAA‑AA‑GGGGCG‑GGTTGGGGGGGGGGTT‑TCTCCCTTTCTTTTTTAT‑CCCCTTTTTGGGTGGAATAcgaga                                              >  1:1828048/1‑147 (MQ=255)
                                                                                                           aTCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTGTC‑CT‑CCT‑AAAAAAA‑AAGCCCC‑CTGTTCA‑CAACCG‑T‑AACAAA‑GGGGCC‑GG‑TGGGTGGGGGGGTATTTCCCCTTATTAATTAT‑TCCATTTTTGGGTGtaaaaaacgcc                                             >  2:2267282/1‑143 (MQ=255)
                                                                                                           aTCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATAcgc‑g                                              <  2:2510954/149‑1 (MQ=255)
                                                                                                           aTCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑gg                                             <  2:1986729/150‑1 (MQ=255)
                                                                                                           aTCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGc                                            <  2:1781423/151‑1 (MQ=255)
                                                                                                            tCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTGCC‑GG‑CAT‑AAAAAAA‑AGGCCCC‑CTTTACA‑CAACTG‑T‑AAC‑AA‑AGGGGC‑GGGTAGGTGGGGGGTTATTTCCGCTCATTAGTCAT‑CCCCTTTTTTGCTGAAAAAAGC‑GGcc                                           >  2:2342522/1‑151 (MQ=255)
                                                                                                              cATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GT‑CAT‑AAAAAAA‑AAGCCCC‑CTTTTCA‑CAAATG‑T‑AAA‑AA‑AGGGGC‑GGGTTGGTGAGGGGTTATTTCCGTTCATTTTTTAT‑CCCCTTTTTGGGTGGAAAAAGC‑GGcccg                                         >  2:1445889/1‑149 (MQ=255)
                                                                                                               aTGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GG‑CAT‑AAAAAAC‑AAGCCCC‑CTTTTCA‑CCAACG‑T‑AAC‑AA‑AGGGGC‑GGGTTGGGGGGGGGTTATTTCCCTTCATTTATTAT‑CCCCTTTTTTGGTGGAATaagagggc                                            >  1:1665071/1‑141 (MQ=255)
                                                                                                                 gCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGccagaaa                                      <  2:1054711/151‑1 (MQ=255)
                                                                                                                 gCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGccagaa                                       <  1:2559654/150‑1 (MQ=255)
                                                                                                                  cAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTTTCC‑GT‑CAT‑AAAAAAC‑AAGCCCC‑CTGTTCA‑CAAATG‑T‑AAA‑AA‑AGGGGC‑GGGTAGGGGAGGGGTTTTTTCCGTTCCTTAGTCAT‑TCCATTTTTGGGTGAAATAAGC‑GGcccgaaa                                      >  2:204695/1‑150 (MQ=255)
                                                                                                                  cAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGccagaaa                                      <  1:204696/150‑1 (MQ=255)
                                                                                                                   aaGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATAcgc                                                <  1:1101830/140‑1 (MQ=255)
                                                                                                                       aTTCACCGCCGGTTTACTGGCGGTTTTTTTTTGCC‑GG‑CCT‑AAAAAAA‑AGGCCCC‑CTTTTCA‑CAAATG‑T‑AAA‑AA‑AGGGGC‑GGGTAGGGGGGGGGGTTTCTCCGTTTTTTTGTCAT‑CCCCTTTTTGGGTGGAAAAAGC‑GGcccgatcggg                                   >  2:865324/1‑143 (MQ=255)
                                                                                                                         tCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGccagaaaaccagaa                               <  2:2517357/150‑1 (MQ=255)
                                                                                                                          cACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGccagaaaaccagaaaa                             <  2:2335129/151‑1 (MQ=255)
                                                                                                                            ccgccgGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTtgt                                                             <  1:292604/118‑1 (MQ=255)
                                                                                                                            ccgccgGTTTACTGGCGGTTTTTTTTCGCC‑CT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGccagaaaacca                                  <  2:1012550/144‑1 (MQ=255)
                                                                                                                                 ggTTTACTGGCGGTTTTTTTTTTCC‑GT‑CAT‑AAAAAAC‑AAGCCCC‑TTTTACA‑CAACTG‑T‑TAC‑AA‑AGGGGC‑GGGTTGGTGAGGGGATATTTTCCTTCATTTGTTAT‑CCCCTTTTTTGGTGGAAAAAGCGGGCCGAAAAACCAAaaaaaaaaat                       >  1:864175/1‑151 (MQ=255)
                                                                                                                                      aCTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGCCAGAAAACCAGAAAATAAAATTATTcc                 <  1:1445884/151‑1 (MQ=255)
                                                                                                                                        tGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGccaga                                        <  2:396866/126‑1 (MQ=255)
                                                                                                                                        tGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGAATATCTCCGTTCATTAGTCAT‑CCCATTTGTGGATGAAATACGA‑GGtcaga                                        >  1:396867/1‑126 (MQ=255)
                                                                                                                                         ggCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGCCAGAAAACCAGAAAATAAAatt                      >  2:2272000/1‑143 (MQ=255)
                                                                                                                                         ggCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGCCAGAAAACCAGAAAATAAAatt                      <  1:2271991/143‑1 (MQ=255)
                                                                                                                                         ggCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGCCAGAAAACCAGAAAATAAAATTATTCCCa               <  1:2267273/150‑1 (MQ=255)
                                                                                                                                         ggCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGCCAGAAAACCAGAAAATAAAATTATTCCCAg              <  1:1907131/151‑1 (MQ=255)
                                                                                                                                               tttttttCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGCCAGAAAACCAGAAAATAAAATTATTCCCAGTACGGc        >  2:2495865/1‑151 (MQ=255)
                                                                                                                                                 tttttCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGCCAGAAAACCAGAAAATAAAATTATTCCCAGTACGGcc       >  2:149937/1‑150 (MQ=255)
                                                                                                                                                 tttttCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGCCAGAAAACCAGAAAATAAAATTATTCCCAGTACGGcc       <  2:1840025/150‑1 (MQ=255)
                                                                                                                                                     tCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGCCAGAAAACCAGAAAATAAAATTATTCCCAGTACGGCCATaa   >  2:2419551/1‑150 (MQ=255)
                                                                                                                                                      cGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGCCAGAAAACCAGAAAATAAAATTATTCCCAGTACGGCCATaaa  <  1:1459944/150‑1 (MQ=255)
                                                                                                                                                      cGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGCCAGAAAACCAGAAAATAAAATTATTCCCAGTACGGCCATaaa  <  1:915976/150‑1 (MQ=255)
                                                                                                                                                      cGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGCCAGAAAACCAGAAAATAAAATTATTCCCAGTACGGCCATaaa  >  1:1642276/1‑150 (MQ=255)
                                                                                                                                                      cGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGCCAGAAAACCAGAAAATAAAATTATTCCCAGTACGGCCATaaa  <  2:1854622/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                                  
AGTTTAAGCTCCGTTTAACATTCATTGAGAAAACTGATGCTACTGTGTCAACGATTCGTTATCAGTGCAGGAAAATGCCTGTTAGCGTAAAAGCAAAACACAAATCTATCCATGCAAGCATTCACCGCCGGTTTACTGGCGGTTTTTTTTCGCC‑GT‑CAT‑AAAAATC‑AGGCCCC‑TTGTACA‑CAACTG‑T‑AAC‑AA‑GGGGCC‑GGTTAGGTGAGGGATTATCTCCGTTCATTAGTCAT‑CCCATTTGTGGCTGAAATACGC‑GGCCAGAAAACCAGAAAATAAAATTATTCCCAGTACGGCCATAAA  >  DH1/2255512‑2255811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑