Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,655,8820CT38.6% 203.3 / 78.1 145F13F (TTC→TTT) ymgDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (12/77);  new base T (56/0);  total (68/77)
Fisher's exact test for biased strand distribution p-value = 1.06e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

TGCTGTCACTGGCGTTCTGACCGGCAATGGCCTCGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGTTGCAGAAATTGTTGCGGTAATGGGTAATGCCAGCGTT  >  DH1/2655735‑2656029
                                                                                                                                                   |                                                                                                                                                      
  cTGTCACTGGCGTTCTGACCGGCAATGGCCTCGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCCGCTTGTTTTTGcca                                                                                                                                                    >  2:18807/1‑148 (MQ=255)
      cACTGGCGTTCTGACCGGCAATGGCCTCGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTTTTTTTGCACCAAt                                                                                                                                                >  2:1128210/1‑150 (MQ=255)
       aCTGGCGTTCTGACCGGCAATGGCCTCGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGa                                                                                                                                              <  1:2342227/151‑1 (MQ=255)
          ggCGTTCTGACCGGCAATGGCCTCGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCt                                                                                                                                           <  1:1958661/151‑1 (MQ=255)
           gCGTTCTGACCGGCAATGGCCTCGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTg                                                                                                                                          <  1:514260/151‑1 (MQ=255)
             gTTCTGACCGGCAATGGCCTCGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTTTTTTTTCTCCCATGAccctgg                                                                                                                                        >  1:1442564/1‑147 (MQ=255)
             gTTCTGACCGGCAATGGCCTCGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTggg                                                                                                                                        <  2:2347371/151‑1 (MQ=255)
                  gACCGGCAATGGCCTCGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGgcg                                                                                                                                      <  1:1641714/148‑1 (MQ=255)
                  gACCGGCAATGGCCTCGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCa                                                                                                                                    <  1:1420852/150‑1 (MQ=255)
                   aCCGGCAATGGCCTCGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAgg                                                                                                                                  <  1:1549827/151‑1 (MQ=255)
                      ggCAATGGCCTCGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCCGTTTGTTTTTGCACCCATGACCTTGGGGCAGGGCt                                                                                                                               >  2:1556586/1‑151 (MQ=255)
                       gCAATGGCCTCGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTa                                                                                                                              <  1:660936/151‑1 (MQ=255)
                            ggCCTCGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGGCTGTTTGTTTTTGCACCAAAGACCTGGGGGCCGGGCTACAat                                                                                                                          >  2:676246/1‑150 (MQ=255)
                               cTCGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTTTTTTTTCACCAAAAACCTTGGCGCAGGGCTACAATA‑tt                                                                                                                      >  2:1157949/1‑150 (MQ=255)
                                 cGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTTTTTGTTTTTGTACCAATGACCTTGGCGCAGGGCTACAATA‑ttta                                                                                                                    >  1:1890394/1‑148 (MQ=255)
                                  gAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGGCCGTTTGTTTTTGCACCAATGACCTGGGGGCAgggagtcaaaatttaa                                                                                                                    >  1:2176023/1‑136 (MQ=255)
                                  gAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑aaaa                                                                                                                 <  2:2457085/151‑1 (MQ=255)
                                   aaGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTTTTTTTGCACCAAAGACCTTGGCGCCGGACTACAATA‑TT‑taaa                                                                                                                 >  2:1891928/1‑150 (MQ=255)
                                    aGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAAc                                                                                                               <  2:2270481/151‑1 (MQ=255)
                                    aGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAAc                                                                                                               <  2:866501/151‑1 (MQ=255)
                                    aGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAAc                                                                                                               <  1:287923/151‑1 (MQ=255)
                                      gggCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGGCTGTTTGTTTTTGCACCAATGACCTGGGGGCAGGGCTACAAAA‑TT‑TAAAAAgg                                                                                                             >  2:2456207/1‑151 (MQ=255)
                                      gggCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACgg                                                                                                             <  1:515664/151‑1 (MQ=255)
                                         cGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTTTTTTTGCACCAAAGAACTTGGCGCCGGGCTACAAAATTT‑AAAAAAGGtt                                                                                                           >  1:55472/1‑151 (MQ=255)
                                         cGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTTGCACCAATAAACTTGGGGCAGGACTACAATA‑TT‑aaaaaaaaatc                                                                                                          >  1:1686085/1‑145 (MQ=255)
                                         cGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAAc                                                                                                               <  2:1686090/146‑1 (MQ=255)
                                         cGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGttt                                                                                                          <  2:1449866/151‑1 (MQ=255)
                                          gATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAg                                                                                                                                   <  1:285450/127‑1 (MQ=255)
                                            ttAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGGCTGTTTGTTTTTTCCACAATAACCCGGGGGCAGGGCTACAAAA‑AT‑aaaaaaaggttttc                                                                                                       >  1:460348/1‑142 (MQ=255)
                                            ttAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCCGTTTTTTTTTGCACCCAAGACCTGGGGGCCGGGCTACAAAA‑TT‑AAAAAAGGGTTT‑cc                                                                                                      >  2:915791/1‑151 (MQ=255)
                                              aaaGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCGGTTTGTTTTCGTCCCAATATCCTGGGGGCCGGAAAACCAAA‑TT‑TAAAACGGTTTG‑CCgg                                                                                                    >  2:1842722/1‑150 (MQ=255)
                                                aGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCa                                                                                                  <  1:1556581/151‑1 (MQ=255)
                                                aGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCa                                                                                                  <  1:1128209/151‑1 (MQ=255)
                                                 ggTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAg                                                                                                 <  2:1133244/151‑1 (MQ=255)
                                                   tGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGGTTGTTTTTGCCCCCAAGACCTGGGGGCCGGACTACAATA‑TT‑AAAAAAGGGTTG‑CCGTCAGa                                                                                                >  2:595850/1‑150 (MQ=255)
                                                    gCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGaaa                                                                                              <  1:18807/151‑1 (MQ=255)
                                                        tGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGGCTGGTTGTTTTTGGACCAATGGACAGGGCGGAGGGATAAAAAA‑TT‑AAAAAAGGGTTGTCCGT‑AGAAACCt                                                                                           >  1:468827/1‑150 (MQ=255)
                                                          aTTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCtata                                                                                        <  2:842612/151‑1 (MQ=255)
                                                             ggCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATAtt                                                                                      <  2:162917/150‑1 (MQ=255)
                                                               cGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACt                                                                                   <  1:1261322/151‑1 (MQ=255)
                                                                   aCGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTTGCACCAATGAACTGGGGGCAGGACTACAATA‑TT‑aaaaaag                                                                                                              >  2:1085373/1‑119 (MQ=255)
                                                                   aCGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCTTGCCCATGACATGGGCGCAGGACTACAATA‑TT‑TAAAACAGGTTG‑TCGTTCGGAACCTCTATTAATTtcg                                                                               >  2:2206255/1‑151 (MQ=255)
                                                                   aCGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACg                                                                                                              <  1:1085372/121‑1 (MQ=255)
                                                                   aCGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTgcg                                                                               <  1:595849/151‑1 (MQ=255)
                                                                   aCGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTgc                                                                                <  1:2458078/150‑1 (MQ=255)
                                                                   aCGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTgc                                                                                <  1:1199202/150‑1 (MQ=255)
                                                                    cGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTgcgc                                                                              <  1:1063013/151‑1 (MQ=255)
                                                                    cGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTgcgc                                                                              <  1:1891923/151‑1 (MQ=255)
                                                                    cGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTgcgc                                                                              <  1:2375579/151‑1 (MQ=255)
                                                                     gggTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCa                                                                             <  2:1095809/151‑1 (MQ=255)
                                                                        tggtgCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTTGCACCAATGACCTGGGCGCAGGGCTACAAAA‑TT‑AAAAAAGGGTTT‑TCCTCAGAAACCTATATTACTTTCGCaaa                                                                           >  1:2101969/1‑148 (MQ=255)
                                                                        tggtgCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTTGCACCAATGAACTGGGCGCAGGACTACAATA‑TT‑TAAAAAGGTTTG‑CCGTCAGAAACCTATATTACTTTCGCAGa                                                                           >  2:1459370/1‑150 (MQ=255)
                                                                        tggtgCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTa                                                                                     <  2:1915241/140‑1 (MQ=255)
                                                                            gCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCa                                                                      <  1:1157948/151‑1 (MQ=255)
                                                                            gCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCa                                                                      <  1:1842717/151‑1 (MQ=255)
                                                                              aaTTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCaa                                                                     <  2:280842/150‑1 (MQ=255)
                                                                               aTTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTTTTTTTTCACCAAAGACCTGGGCGCCGGACTACAATA‑TT‑AAAAAAGGTTTT‑CCGTCAGAAACCTATATTTCTTTTGCAGAAGCCAACg                                                                   >  2:778537/1‑151 (MQ=255)
                                                                               aTTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACg                                                                   <  1:778536/151‑1 (MQ=255)
                                                                                ttCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTTACACCCATGACCCGGGGGCAGGGCTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAGAACTATATTACTTGCACAggaaacaaac                                                                   >  2:268945/1‑141 (MQ=255)
                                                                                   tGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTTGCACCAATGACCTGGGGGCAGGACTACAATA‑TT‑AAAAAAGGGTTG‑CCGTCAGAAACCCATATTACTTGCGCAGAAGCCAAAGaaa                                                                >  2:617362/1‑150 (MQ=255)
                                                                                     ggTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATgg                                                             <  1:915790/151‑1 (MQ=255)
                                                                                       tAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGGCTGGTTGTTTTTGCACCAATAAAATTGGGGCAGGACTACAATA‑TT‑AAAAAAGGTTTT‑TCGTCAGAAAACTATATTAATTGTGCAGaaaaatggaagagagtg                                                           >  1:1428925/1‑136 (MQ=255)
                                                                                       tAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGGGCAGGACTACAATA‑TT‑AAAAAAGGTTTG‑CCGTCAGAAACCCATATTTCTTTCGCAGAAGCCAAc                                                                    >  2:1970415/1‑142 (MQ=255)
                                                                                       tAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGa                                                                           <  2:1428930/135‑1 (MQ=255)
                                                                                        aaaaTGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCaa                                                          <  2:119129/151‑1 (MQ=255)
                                                                                         aaaTGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCaaa                                                         <  2:55472/151‑1 (MQ=255)
                                                                                          aaTGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGGCTGTTTGTTTTTTCACCAATGAACTGGGGGCAGGACTACAATA‑TT‑AAAAAAGGGTTG‑CCGTCAGAAACCCATATTACTTTCGCAGAAACCAACGAAAAGGCaaa                                                         >  1:2111527/1‑150 (MQ=255)
                                                                                          aaTGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTTTTTTTGCACCAATAACGTGGGCGCAGGACTACAATA‑TTAAAAAACAGTTGG‑CGCTAGGAAACC‑ATATTACTTTTGGCAAAGCCAACGAAAAGGTaaa                                                         >  1:1530401/1‑150 (MQ=255)
                                                                                          aaTGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTTGCACCAATGAACTGGGCGCAGGACTACAATA‑TT‑AAAAAAGGTTTG‑CCGTCAGAAAACTATATTTCTTTCGCAGAAACCAACGAAAAGGCaaa                                                         >  1:1652036/1‑150 (MQ=255)
                                                                                            tGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTTTTTTTGCACCAAAGAACTTGGGGCAGGGCTACAATA‑TT‑TAAAAAGGGTTT‑TCGTCAGAAAACTATATTACTTGCGCAGAAACCAAACAAAAGGCaaaaa                                                       >  1:1000487/1‑150 (MQ=255)
                                                                                             gAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGGCTGTTTTGTTTTGGACCAATAACCTTTGCGGCGGACTCCAATA‑TT‑AAAAACAGATTG‑GCGCGAGCAACCCCTATTATTTGTGCAGAAGCCAACCAAAAGGCaaaaaat                                                     >  1:2508643/1‑149 (MQ=255)
                                                                                             gAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTTTCACCAAAAAACTTGGGGCAGGAATACAATA‑TT‑AAAAAAGGGTTT‑TCGTCAGAAAACTATATTAATTTCGCAGAAACCaaaaaaaaaggaaaaaa                                                      >  1:854913/1‑135 (MQ=255)
                                                                                             gAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCa                                                           <  1:2000946/145‑1 (MQ=255)
                                                                                              aaaTAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTTTTTTTGCACCAATGACCTTGGCGCAGGACTACAATA‑TT‑AAAAAAGGGTTG‑CCGTCAGAAACCTATATTACTTTCGCAGAAACCAACGAAATGGCAAAAACTg                                                    >  2:329409/1‑151 (MQ=255)
                                                                                              aaaTAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTTCCCCCAATAAACTCGGCGCCGGGGTACAAAA‑TT‑AAAAAAAGTTTG‑TCGTCAGAAAACTATATTAATTTACCAAAAGCCAACGAAAAgggcagaatgg                                                    >  1:11007/1‑142 (MQ=255)
                                                                                                 tAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTTGCACCAAAGACCTGGGGGCCGGACTACAAAA‑TT‑AAAAAAGGTTTG‑CCGTCAGAAACCTATATTTCTTTCGCAGAAGCCAACGAAAAGGCAAAAAATGAc                                                  >  2:1639301/1‑150 (MQ=255)
                                                                                                  aaGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACa                                                 <  2:2176031/150‑1 (MQ=255)
                                                                                                    ggAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTTGCACCAATGACCTTGGCGCAGGACTACAATA‑TT‑AAAAAAAGTTTG‑CCGTCAGAAAACTATATTACTTGCGCAGAAACCAAAGAAAAGGCAAAAAATGACAgcg                                              >  2:2378991/1‑151 (MQ=255)
                                                                                                      aaTGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGca                                            <  2:2508655/151‑1 (MQ=255)
                                                                                                         gatgatgaAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAg                                         <  2:2101977/151‑1 (MQ=255)
                                                                                                         gatgatgaAAAAATTTGCTTTACTGGCGGGCCCGTTTGTTTTTGTGCCCAAGACATGGGGGCCGGGCTTCAAAA‑TT‑AAAAAAGGTTTG‑TCGTCAGCAAACTCTATTTCTTGAGCGGAAACCACAGGAAAGGaaaaaaaggaaagccccca                                          >  1:1947476/1‑137 (MQ=255)
                                                                                                          atgatgaAAAAATTTGCTTTACTGGCGGGGCTGTTTGTTTTTGCACCAAAAACCTTGGGGCCGGACTAAAATA‑TT‑AAAAAAGGGTTG‑CCGTCAGAAACCTATATTACTTTCGCAGAAAGCAACGAAATGGCaaaaaatcaaaacggccagg                                        >  1:716429/1‑136 (MQ=255)
                                                                                                            gatgaAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGtt                                      <  1:2562124/151‑1 (MQ=255)
                                                                                                               gaAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGTTGc                                    <  1:2456195/150‑1 (MQ=255)
                                                                                                                aaaaaaTTTGCTTTACTGGCGGGCCCGTTTGTTTTTTCACCAAAGAACTTGGCGCAGGACTACAATA‑TT‑TAAAAAGGGTTT‑CCGTCAGAAACCTATATTACTTGCGCAGAAAACAAACAAAAGGCAAAAAATGACAACGcacaaggtgt                                    >  1:926542/1‑143 (MQ=255)
                                                                                                                       ttGCTTTACTGGCGGGCCTGTTTGTTTTTGCACCAATGACCTGGGCGCAGGACTACAAAA‑TT‑AAAAAAAGTTTT‑CCCTCAGAAACCTATATTACTTGCGCAGAAACCAACGAAAAGGCAAAAACTGACAGCGCACAGGGTGCCGAAAttgt                           >  1:1632765/1‑151 (MQ=255)
                                                                                                                       ttGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGTTGCAGAAAttg                            <  1:617361/150‑1 (MQ=255)
                                                                                                                        tGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGTTGCAGAAAttgt                           <  2:1652041/150‑1 (MQ=255)
                                                                                                                            ttACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGTTGCAGAAAttgt                           <  2:1412324/146‑1 (MQ=255)
                                                                                                                              aCTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGTTGCAGAAATTGTTGCggta                    <  2:1530406/151‑1 (MQ=255)
                                                                                                                               cTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGTTGc                                    >  1:115144/1‑134 (MQ=255)
                                                                                                                               cTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGTTGCAGAAATTGTTGCggta                    >  1:2411381/1‑150 (MQ=255)
                                                                                                                                tGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGTTGCAGAAAt                              <  2:2108592/139‑1 (MQ=255)
                                                                                                                                 ggCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGTTGCAGAAATTGTTGCggtaa                   <  2:709632/149‑1 (MQ=255)
                                                                                                                                       ccTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGTTGCAGAAATTGTTGCggtaatgggtaat           >  2:2459778/1‑151 (MQ=255)
                                                                                                                                       ccTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGTTGCAGAAATTGTTGCggtaatgggtaat           <  2:483867/151‑1 (MQ=255)
                                                                                                                                           tttgtttTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGTTGCAGAAAtt                             <  2:29845/129‑1 (MQ=255)
                                                                                                                                           tttgtttTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGTTGCAGAAATTGTTGCGGTAATGGGTAATGcc        >  1:641633/1‑150 (MQ=255)
                                                                                                                                              gtttTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACGGACAGCGCACAGGTTGCAGAAATTGTTGCGGTAATGGGTAATGCCAGc     <  2:1890399/150‑1 (MQ=255)
                                                                                                                                                tttCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGTTGCAGAAATTGTTGCGGTAATGGGTAATGCCAGCGt   <  2:468827/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
TGCTGTCACTGGCGTTCTGACCGGCAATGGCCTCGAAGGGGCGATTAAAGGTGCCGTGATTGGCGGTACGGGTGGTGCAATTCTGGGTAAAATGAAATAAGGAATGATGATGAAAAAATTTGCTTTACTGGCGGGCCTGTTTGTTTTCGCACCAATGACCTGGGCGCAGGACTACAATA‑TT‑AAAAACGGTTTG‑CCGTCAGAAACCTATATTACTTGCGCAGAAGCCAACGAAATGGCAAAAACTGACAGCGCACAGGTTGCAGAAATTGTTGCGGTAATGGGTAATGCCAGCGTT  >  DH1/2655735‑2656029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑