Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,716,3620CA36.7% 243.0 / 53.1 120E40* (GAA→TAA) thiKthiamine kinase
Reads supporting (aligned to +/- strand):  ref base C (70/6);  new base A (0/44);  total (70/50)
Fisher's exact test for biased strand distribution p-value = 1.19e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

CGACTACCATCCAGTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACGGCACCTTTACCTGACCAGATAATTTCGCCCGT  >  DH1/2716212‑2716507
                                                                                                                                                       |                                                                                                                                                  
cGACTACCATCCAGTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTc                                                                                                                                                    >  2:1341202/1‑151 (MQ=255)
cGACTACCATCCAGTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTc                                                                                                                                                    >  1:354469/1‑151 (MQ=255)
 gACTACCATCCAGTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTc                                                                                                                                                    >  2:198880/1‑150 (MQ=255)
 gACTACCATCCAGTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTc                                                                                                                                                    >  2:2619093/1‑150 (MQ=255)
    tACCATCCAGTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCaa                                                                                                                                                  >  1:2384859/1‑149 (MQ=255)
    tACCATCCAGTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCaa                                                                                                                                                  <  2:2384869/149‑1 (MQ=255)
    tACCATCCAGTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATg                                                                                                                                                >  2:2321893/1‑151 (MQ=255)
     aCCATCCAGTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATg                                                                                                                                                >  2:572874/1‑150 (MQ=255)
      ccATCCAGTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATg                                                                                                                                                >  1:2058147/1‑149 (MQ=255)
        aTCCAGTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATgaga                                                                                                                                             >  1:1050120/1‑150 (MQ=255)
         tCCAGTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATga                                                                                                                                               <  2:1288273/147‑1 (MQ=255)
         tCCAGTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGATGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGaa                                                                                                                                            >  2:2170532/1‑150 (MQ=255)
          ccAGTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGaaa                                                                                                                                           >  1:2187966/1‑150 (MQ=255)
            aGTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑Ct                                                                                                                                       <  1:1904226/151‑1 (MQ=255)
             gTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTc                                                                                                                                      >  1:2091047/1‑151 (MQ=255)
                   gagaTATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCa                                                                                                                                <  1:86365/151‑1 (MQ=255)
                           aaTGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGccc                                                                                                                        >  1:443964/1‑151 (MQ=255)
                                ggtttCGGTGCAATGAGGGGGGGTTTTTGTGAAAACCCCCGATAGTGGCGCAAAACCGCGGCCCGCGGGAAATCGGGACCGGGCGGCTACCGCAAAAAAAACCGCTGCCCTTTA‑TGTAAAATAAAAAAC‑CTCCCGCCACTAAGCCCACTAt                                                                                                                   <  2:2138285/148‑1 (MQ=255)
                                   ttCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAg                                                                                                                           >  2:1328147/1‑140 (MQ=255)
                                    tCGGTGCAATGCAGGGGGGTAGTTGTGAAAAAGCCCGATACGGGCGAAAGACCGCGGCCTGGGGCGCACAAGGTTCGTGCGGCTGCCCCAAAAAAAACCGCTGGCCTTGA‑TGTTAAATGAAAAAC‑CCCCCGCCACTAAGCCCACTATTa                                                                                                                 <  2:354468/149‑1 (MQ=255)
                                    ggggggaaaTGCAGGGGGGTAGTTGTAAAAAGCCCCGATCCGGGCGAAAAAACGGGGCCGGCGGCGAATAAGGATGGGGCGGCTGACGCAAAAAAAACCGCGGGCCTGGA‑TGTAAAAAGAAAAAC‑CTCCCGCCACTAAGCCCACTATTaa                                                                                                                <  2:1167456/143‑1 (MQ=255)
                                     cGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACt                                                                                                                     >  2:371421/1‑144 (MQ=255)
                                     cGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAAc                                                                                                              >  2:366223/1‑151 (MQ=255)
                                       gTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAAcgt                                                                                                            >  1:1993112/1‑151 (MQ=255)
                                         gaaatgaaggggggTAGTTGTAAAAAAGCCCGATACGGGCGAAAGAACGCGGCCTGCGGCGAATCAGGATCGGGCGGCTGCCGCAAAAAAAACCGCGGGCCTTGA‑TTTTAAAGAAAAAAA‑CTCCCGCCACTAAGCCCACTATTAAAcgtc                                                                                                           <  2:2113711/139‑1 (MQ=255)
                                           aaTGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTg                                                                                                        >  1:2278448/1‑151 (MQ=255)
                                            atgcatgcGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTg                                                                                                        >  2:1162472/1‑150 (MQ=255)
                                              gcatgcGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACg                                                                                                     >  1:1226143/1‑151 (MQ=255)
                                               catgcGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACgg                                                                                                    >  2:358121/1‑151 (MQ=255)
                                                 tgcGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACggg                                                                                                   >  1:1340635/1‑150 (MQ=255)
                                                 tgcGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACggg                                                                                                   >  2:83549/1‑150 (MQ=255)
                                                 tgcGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACggg                                                                                                   >  2:625470/1‑150 (MQ=255)
                                                      gTAGTTGTAAAAAACCCGGATACGGGCGAAAGAACGGGGACGGCGCCGCAACAGGATCGGGCGGCTGCCGCACAAAAAACCGCTGGCCTTGT‑TGTAAAATGAAAAAC‑CCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATa                                                                                             <  2:2091055/151‑1 (MQ=255)
                                                        aGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACt                                                                                           >  1:49759/1‑151 (MQ=255)
                                                         gtttaaaaaaaCCCAGATACTGGAGCAAAAAGGGGGACGGCGGCGCATCAGGTCCGGGCGGCTCACCCAAAAAAAACCGGTGGCCCTGA‑TTTAAAATAAAAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTg                                                                                          <  1:198881/143‑1 (MQ=255)
                                                              gATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCggg                                                                                      >  2:1978157/1‑150 (MQ=255)
                                                              aaaaaaGCCCAATACTGCAGAAAGAGCGGGGACTCCGGCAAAACAGGATCGTGGGGCAGACGCAAAAAAAACCGCGGGCCTTGA‑TTTTAAAGAAAAAAC‑CCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCggg                                                                                      <  1:575509/147‑1 (MQ=255)
                                                               aaacaccccGATACGGGGGAAATACCGGGGCCGGCGCCGAAGAAGGATCGGGGGGCGGACGCACAAAAAACCGCCGCCCCTGA‑TGTAAAATGAAAAAC‑CCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGaa                                                                                    <  1:358122/145‑1 (MQ=255)
                                                                aaaaGCCCAATCCTGGAGAAAGACGGCGGACGGGGGCACATAAGGATCGGGCGGCTGACCAAAAAAAAACCGTTGGCCTTG‑‑TTTTAAAAGAAAAAACCCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGaaa                                                                                   <  1:1031544/150‑1 (MQ=255)
                                                                   aGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGaaaa                                                                                  >  1:2427826/1‑149 (MQ=255)
                                                                    gCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACggg                                                                                                   >  1:2554988/1‑131 (MQ=255)
                                                                     ccagATACGGGCGAAAAACCGCGGGCTGGGGCGCATGGGGATCGGGGGGCTGCCGAACAAAAAAGCGGTGCCCTTGA‑TTTTAAAGAAGAAAC‑CGCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAgcg                                                                              <  2:1050121/148‑1 (MQ=255)
                                                                      ccGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAgcgc                                                                             >  2:864931/1‑151 (MQ=255)
                                                                      ccGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAgcgc                                                                             >  2:2272937/1‑151 (MQ=255)
                                                                      ccGATACGGGCAAAAGAGCGCGGACGGCGGCGCATCAGGACCGGGCGGCTGACCCACAAAAAACCGCTGGCCTTGT‑TGTTAAATAAAAAAA‑CCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAgcgc                                                                             <  1:1162471/151‑1 (MQ=255)
                                                                       gattccgggCGAAAAAGCGGGGACGGCGGCGCATCGGGATGGGGCGGCTGACCCAAAAAAAACCGCGGGCCCTTA‑TTTTAAATGAAAAAC‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGcgcg                                                                            <  2:2278457/144‑1 (MQ=255)
                                                                        gctACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGcgcg                                                                            >  2:1719136/3‑150 (MQ=255)
                                                                        gATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGaaaa                                                                                  >  1:127434/1‑144 (MQ=255)
                                                                        gATACGGGCAAAAAAACGCGGACGGCGGCGCATCAGGTTCGGGCGGCTGACCCACAAAAAACCGCGGCCCTTGA‑TGTAAAATAAAAAAA‑CCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGaaaa                                                                                  <  2:127434/144‑1 (MQ=255)
                                                                         aTACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGAc                                                                          >  1:1258074/1‑151 (MQ=255)
                                                                           aCTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGAc                                                                          >  2:2494790/1‑149 (MQ=255)
                                                                           aCTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGAc                                                                          >  2:337299/1‑149 (MQ=255)
                                                                            cctgcacAAGACCGCGGCCGGCGGCCCACCAGGATCGGGCGGCTGCCACAAAACAAACCGCTGCCCTTGA‑TGTTAAAAAAAAAAA‑CCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGc                                                                       <  2:1839847/145‑1 (MQ=255)
                                                                              ggcaaaaGAACGCGGACTGCGCCGCAACAGGCTCGTGCGGCTGACCCACAAAAAAACGCGGCCCCTGA‑TTTAAAAAGAAAAAC‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCaa                                                                     <  2:443963/146‑1 (MQ=255)
                                                                              ggCGAAAGAAGGCGGCGTGCGGCGCATCAGGTTGGGGCGGCTGCCGCACAAAAAAACGCGGGGCTTGA‑TTTTAAATGAAAAAC‑CCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGc                                                                       <  2:1191692/149‑1 (MQ=255)
                                                                                cgaaaaaccgcgGACTGGGGCGCACAAGGATGGGGCGGCTGCCGCAAAAAAAACCGC‑GGCCCTTG‑TTTTAAAAGAAAAAACCCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAAtt                                                                   <  2:49759/143‑1 (MQ=255)
                                                                                 gcAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAgc                                                                               >  1:139639/1‑138 (MQ=255)
                                                                                 gcAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATtc                                                                  >  1:1238175/1‑151 (MQ=255)
                                                                                  cAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATtcg                                                                 >  1:2546364/1‑151 (MQ=255)
                                                                                   aaGAACGGGGACGGCGGCGAATCAGGTTCGGGCGGCTGACCCAAAAAAAACCGCGGCCCCTAA‑TGTTAAAGAAAAAAC‑CCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATtcgt                                                                <  2:611261/151‑1 (MQ=255)
                                                                                   aaGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATtcgt                                                                >  1:611260/1‑151 (MQ=255)
                                                                                   aaGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATtcgt                                                                >  1:82940/1‑151 (MQ=255)
                                                                                   aaGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATtcgt                                                                >  2:508335/1‑151 (MQ=255)
                                                                                    aGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATtcgtc                                                               >  2:74918/1‑151 (MQ=255)
                                                                                     gAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATtcgtcg                                                              >  1:1784115/1‑151 (MQ=255)
                                                                                       aCGCGGACGGCGGCGCATGAGGATCGTGCGGCTGACGCAAAAAAAACCGCTGCCCCTGA‑TGTTAAAAAAAAAAC‑CCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTcgcg                                                            <  1:864930/151‑1 (MQ=255)
                                                                                        cgcgGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTcgcgc                                                           >  2:1621648/1‑151 (MQ=255)
                                                                                        cctatcttGCGGCGCATCAGATTGGGGCGGC‑GAACCAAAAAAAAACCGCGGCCCTTATTGTTCAAAAAAAAAC‑CCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTcgcg                                                            <  2:686330/143‑1 (MQ=255)
                                                                                           ggACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTg                                                        >  2:2490202/1‑151 (MQ=255)
                                                                                             aCTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGAt                                                      >  2:27928/1‑151 (MQ=255)
                                                                                              cgggggcgAATCAGGATCGGGGGGCTCACGCAAAAAAAACCGCGGCCCTTGT‑TTTAAAATAAAAAAC‑CCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATg                                                     <  2:2427838/147‑1 (MQ=255)
                                                                                               tGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTcgcg                                                            >  1:686329/1‑143 (MQ=255)
                                                                                                 cggcgCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATggg                                                   >  1:1157953/1‑150 (MQ=255)
                                                                                                  ggcgCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGat                                                 >  1:2391078/1‑151 (MQ=255)
                                                                                                  ggcaCATAAGGATCGGGGGGCTGACACAAAAAAAAACGCGGGCCTTGA‑TTTAAAATAAAAAAC‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGat                                                 <  2:1157954/151‑1 (MQ=255)
                                                                                                    ccatagagggtggTGCGGCTGACCCAAAAAAAACCTCGGCCCCTGT‑TATAAAAAGAAAAAT‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGatta                                               <  2:1238177/139‑1 (MQ=255)
                                                                                                     gcATCTGGATGGGGGGGCTGCCCCAAAAAAAACCGCTGGCCCTAT‑TTTAAAATAAAAAAC‑CCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGattat                                              <  2:1993120/151‑1 (MQ=255)
                                                                                                     gcATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGat                                                 >  1:526904/1‑148 (MQ=255)
                                                                                                       aTCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATtg                                            >  1:1537569/1‑151 (MQ=255)
                                                                                                         aaGGATCGTGCGGCTCACACACAAAAAAACGCTGCCCTTTA‑TGTTAAATGAAAAAC‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATtgct                                          <  1:625469/150‑1 (MQ=255)
                                                                                                            gATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGc                                                                       >  1:1065234/1‑119 (MQ=255)
                                                                                                              tCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGa                                      >  1:1781863/1‑150 (MQ=255)
                                                                                                               cGTGCGGCGGACGCACAAAAAAGCGCTGGCTCTGT‑TTTAAAATAAAAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGaaa                                    <  1:1341199/151‑1 (MQ=255)
                                                                                                                 ggcgcCTCACCCACAAAAAACCGCGGGCTCTGA‑TTTAAAATGAAAAAC‑CCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACg                                  <  2:1668951/150‑1 (MQ=255)
                                                                                                                    ggCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACGGc                                >  1:1701779/1‑150 (MQ=255)
                                                                                                                      cTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACGGc                                >  1:570783/1‑148 (MQ=255)
                                                                                                                      cTGACCCAAAAAAAACCGCTGCCCCTGA‑TTTAAAATGAAAACC‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACGGc                                <  2:570784/148‑1 (MQ=255)
                                                                                                                       tGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGattat                                              >  1:1911395/1‑133 (MQ=255)
                                                                                                                       tGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACGGCACCt                            >  2:2517345/1‑151 (MQ=255)
                                                                                                                        gACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATtgct                                          >  1:911252/1‑136 (MQ=255)
                                                                                                                          cccacaAAAAAACGCGGGCCTTAT‑TGTTAAATGAAAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACGGCACCTTTa                         <  2:1226145/149‑1 (MQ=255)
                                                                                                                           gCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACGGCACCTTTAc                        >  2:2339988/1‑151 (MQ=255)
                                                                                                                            cacaACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACGGCACCTTTAcc                       >  1:1693061/1‑151 (MQ=255)
                                                                                                                                 aaaacCGCTGCCCTTAA‑TGTAAAAAGAAAAAC‑CCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACGGCACCTTTACCTGAcc                  <  2:1340638/150‑1 (MQ=255)
                                                                                                                                     ccgcgGGCCTTGA‑TTTTAAATAAAAAAC‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACGGCACCTTTACCTGACCAGAt              <  2:1537574/150‑1 (MQ=255)
                                                                                                                                     aggcgggCCATGA‑TGTTAAATAAAAAAC‑CCCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACGGCACCTTTACCTGACCAGAt              <  1:366224/146‑1 (MQ=255)
                                                                                                                                          ggCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACGGCACCTTTa                         >  2:312122/1‑135 (MQ=255)
                                                                                                                                           gCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACGGCACCTTTACCTGACCAGATAATTTc        >  2:513523/1‑151 (MQ=255)
                                                                                                                                              cTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACGGCACCTTTACCTGACCAGATAATTTCGcc     >  2:1235502/1‑151 (MQ=255)
                                                                                                                                                 a‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACGGCACCTTTACCTGACCAGATAATTTCGCCCGt  >  1:369097/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                  
CGACTACCATCCAGTCACGGAGATATAAATGCGGCTTCGGTGCAATGCATGCGGGTAGTTGTGATAAAGCCCGATACTGGCGCAAGAACGCGGACTGCGGCGCATCAGGATCGTGCGGCTGACGCACAACAAAACGCTGGCCCTGA‑TGTTCAATGAGAAAA‑CTCCCGCCACTAAGCCCACTATTAAACGTCGTGACGGGATGATACTGCGGGAAAAAGCGCGACAGCAATTCGTCGCGCGTGATGGGATTATTGCTGCGAAACGGCACCTTTACCTGACCAGATAATTTCGCCCGT  >  DH1/2716212‑2716507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑