Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,726,1660AC44.4% 181.4 / 87.1 127Y92D (TAC→GAC) pabC4‑amino‑4‑deoxychorismate lyase
Reads supporting (aligned to +/- strand):  ref base A (68/2);  new base C (0/56);  total (68/59)
Fisher's exact test for biased strand distribution p-value = 5.93e-34
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

GATGTTTAATACCTGCAAGATGAGGATTGCGCCCCAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGACAAGCATCCTGTAGTCGCTGGATATG  >  DH1/2726016‑2726316
                                                                                                                                                           |                                                                                                                                                      
gaTGTTTAATACCTGCAAGATGAGGATTGCGCCCCAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTa                                                                                                                                                        <  1:1033285/151‑1 (MQ=255)
 aTGTTTAATACCTGCAAGATGAGGATTGCGCCCCAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTAc                                                                                                                                                       <  2:1710023/151‑1 (MQ=255)
      tAATACCTGCAAGATGAGGATTGCGCCCCAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTc                                                                                                                                                   <  1:1241471/150‑1 (MQ=255)
          aCCTGCAAGATGAGGATTGCGCCCCAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTcgcccg                                                                                                                                              >  1:655257/1‑151 (MQ=255)
              gCAAGATGAGGATTGCGCCCCAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCAc                                                                                                                                          >  2:468080/1‑151 (MQ=255)
                aaGATGAGGATTGCGCCCCAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACt                                                                                                                                         >  1:571179/1‑150 (MQ=255)
                aaGATGAGGATTGCGCCCCAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACt                                                                                                                                         >  1:2299602/1‑150 (MQ=255)
                aaGATGAGGATTGCGCCCCAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACt                                                                                                                                         >  2:492096/1‑150 (MQ=255)
                  gATGAGGATTGCGCCCCAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTAcc                                                                                                                                      >  2:1158949/1‑151 (MQ=255)
                    tGAGGATTGCGCCCCAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACcgc                                                                                                                                    >  1:2523941/1‑151 (MQ=255)
                    tGAGGATTGCGCCCCAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACcg                                                                                                                                     >  2:205846/1‑150 (MQ=255)
                        gttggccccccACCCGCCCCGGGTTGAGCCCAAACATACCCCCCTCTTTACAAAAACGGTG‑GTAATGGGGAGGAAAAGCCGAAA‑GGGAGAAAACCCGTGTT‑GCC‑GGTCCGTTGTTAAA‑GGTGCGGTCCCCTCGCCCGCCACTACCGCGACt                                                                                                                                <  2:2619212/144‑1 (MQ=255)
                           tGCGCCCCAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGAt                                                                                                                             >  2:1616434/1‑151 (MQ=255)
                           tGCCCCCCAGCCGCACCGGGTTAACCCCTAAGTTAACCCCCTCTTTCCGAAAACGTTG‑GAAATGTGAAGAATAAGCCGAAA‑GGGAGAAAACCCGCTTG‑CCC‑GTTCCGTTGTTAAA‑TGGCCTGTCCCCTCGCCCGCCACTACCGCGACTGa                                                                                                                              <  1:574166/150‑1 (MQ=255)
                           cgccccccGGCCGCACGGGGTTTAGCGCCACCGTACCCCCCTCGTTACGAAAACGGTC‑GTAATGTGCAGAAAAACCCGTAA‑GGGAGAGAA‑CCGGGTTGGCG‑GGCCCGTTGTTAAA‑TGTGCTGTCCCCCCGCCCGCCACTACCGCGACTGa                                                                                                                              <  1:1771382/146‑1 (MQ=255)
                              gcCCCAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑TGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCAc                                                                                                                          >  1:2633649/1‑151 (MQ=255)
                               ccccAGCCGCCCGGGGCTTGGCGCAAACGAAACCCCCTCGTTACGAAACGGGTG‑GTATGGTGAAGAAAAAGCCGAAA‑GGGAGAGAACCCGCGTT‑GCC‑GTTCCGTTTTTAAA‑TGTGCTTCCCCCCCGCCCGCCACTACCGCGACTGATCACg                                                                                                                         <  2:1625666/151‑1 (MQ=255)
                               ccccAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACg                                                                                                                         >  1:520276/1‑151 (MQ=255)
                                cgatcccGCCCGGGGCTTAGCCCCAACTTAACCCCCTCGTTCCGAAAACGGTC‑TTAATGTGAGGGAAAAGCCGTAA‑GGGAGAAAACCCGCTTT‑GCC‑GGTCCGCTGTTAAA‑TGTGCTGTCCCCTCGCCCGCCACTACCGCGACTGATCACGa                                                                                                                        <  2:1672328/146‑1 (MQ=255)
                                cccAGCCGCCCGGGGCTTAGCGCAAACGTAACCCCCTCGTTCCGCAAACGGTC‑GTAATGTGCAGGATAAGCCGAAA‑CGGAGAGAACCCGCGTT‑GCC‑GGTCCGCTTTTCAA‑TGTGCTGCCCCCCCGCCCGCCACTACCGCGACTGATCACGa                                                                                                                        <  2:2147107/151‑1 (MQ=255)
                                cccAGCCGCCCCGGGCTGGGCGCAAACGTAACCCCCTCTTTCCCAAACCGGTC‑GTAATGGGCAGGATAAGCCGAAA‑CGGAGAGACCCCGCGTT‑GCC‑GCCCCGTTTTTAAA‑TGGGCTGCCCCCCCGCCCGCCACTACCGCGACTGATCACGa                                                                                                                        <  2:50474/151‑1 (MQ=255)
                                 ccAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGAc                                                                                                                       >  2:1881094/1‑151 (MQ=255)
                                   aGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACtt                                                                                                                     >  1:1300704/1‑151 (MQ=255)
                                       gCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTc                                                                                                                   >  2:2010537/1‑149 (MQ=255)
                                        ccccGGGTTTCGCGCCAACCTAACCCCCTGTTTCCAAAACCGGCG‑GAAAGGTCAAGGATAACCCGAAC‑GGGAAAAATTCCGCTTG‑GCG‑GGCCCGCTGTTCAG‑TGGTCTGCCCCCCCGCCCGCCACTACCGCGACTGATCACGACTTTCAGc                                                                                                                <  2:655258/149‑1 (MQ=255)
                                        cACGGGTCTTAGCCCCAACGTAACCCCCTCTTTACCAAAGCGGTC‑GTAAGGTGCAGAATAAGCCGTAA‑GGGAGAGACCCCGCGTT‑GCC‑GGCCCGTTGTTAAT‑TGTGCTGTCCCCCCGCCCGCCACTACCGCGACTGATCACGACTTTCAGc                                                                                                                <  2:1300706/151‑1 (MQ=255)
                                         aCCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGc                                                                                                                >  1:1614624/1‑150 (MQ=255)
                                             ggCTTGGCCCCACCTTACCCCCCTCGTTCCGCAACCGGTC‑GTAATGTGCAGAAAAACCCGTAA‑CGGAGAGACCCCGCGTT‑GCC‑GGTCCGCTTTTCAT‑TGTGCTGCCCCCCCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACAcc                                                                                                           <  2:520276/151‑1 (MQ=255)
                                             ggCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACAcc                                                                                                           >  1:652061/1‑151 (MQ=255)
                                                ttAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACttt                                                                                                                    >  2:1973178/1‑139 (MQ=255)
                                                ttAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCAtt                                                                                                        >  2:247775/1‑151 (MQ=255)
                                                 aagcccaaaCTTAACCCCCTCTTTACAAAAACGGTC‑GTAATGTGAAGGAAAAGCCGTAA‑GGGAGAGAACCCGCGTT‑GCC‑GGCCCGTTGTTAAT‑TGTGCTGTCCCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCAttt                                                                                                       <  2:2644404/144‑1 (MQ=255)
                                                  agcccaaaCTTAACCCCCTCGTTACGAAACCGGTG‑TTAATGTGCAGAAAAAGCCGAAA‑CGGAGAGAACCCGCGTT‑GCC‑GGTCCGCTTTTAAA‑TGTGCTGTCCCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCAtttt                                                                                                      <  1:1863654/145‑1 (MQ=255)
                                                    cgcCAACTTACCCCCCTCTTTACGAAAACGTTC‑GTAATGTGCAGAATAAGCCGTAA‑CGGAGAGAACCCGCGTT‑GCC‑GGTCCGTTTTTCAA‑TGTGCTGTCCCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGc                                                                                                    <  2:571180/151‑1 (MQ=255)
                                                     gcCAACTAAACCCCCTCGTTCCGAAACCGGTC‑GTATTGGCAAGGTAAAGCCGTAA‑GGGAAAGACCCCGCTTC‑GCC‑GCTCCGCGGTTAAA‑GGTCCTTTCCCCCCCCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCt                                                                                                   <  1:2355739/151‑1 (MQ=255)
                                                       cAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGt                                                                                                 >  1:2200736/1‑151 (MQ=255)
                                                        aaCTTAACCCCCTCGTTACGCAACCGGTC‑GAATTGTGAAGAAAAAGCCGAAA‑GGGAGAAACCCCGCTTT‑GCC‑GTTCCGTTTTTAAT‑TGTGCTTTCCCCCCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGtt                                                                                                <  2:841653/151‑1 (MQ=255)
                                                         aCGTAACCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGAAAAGCCGAAA‑CGGAGAGAACCCGCGTC‑GCC‑GGTCCGTTTTTCAA‑TGTGCTGTCCCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTc                                                                                               <  1:204358/151‑1 (MQ=255)
                                                          caaaacccccTGGTTACGCAAACGGTC‑GTAATGCGCGGTAAACACCGAAA‑GGAAAAGATCCCGCTTG‑ACC‑GTTCGGCTTTCTAT‑TGTGCGCCCCCCCCGTCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTct                                                                                              <  1:267777/145‑1 (MQ=255)
                                                              ccccccTCTTTACCAAAGCGGTC‑GTAATGGGCAGGAAAAGCGGAAA‑GGGAGAGAACCCGCTTT‑GCG‑GGCCCGCTTTTAAA‑TGTGCTTTCCCCCCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTctgctg                                                                                          <  2:1813538/149‑1 (MQ=255)
                                                               tCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCtgctgc                                                                                         >  1:2284138/1‑151 (MQ=255)
                                                               cccccTCGTT‑CCCAAACGGTCTTTATTGTGCGGAATAGCCCGAAA‑CGGAGAAACCCCGCTTT‑GCC‑GGCCCGTTTTTCAA‑GGTCCGCTCCCCGCCCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTctgctg                                                                                          <  2:2059524/149‑1 (MQ=255)
                                                                ccccTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGcc                                                                                        >  1:1657589/1‑151 (MQ=255)
                                                                ccccTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGcc                                                                                        >  1:1071541/1‑151 (MQ=255)
                                                                  ccTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGcc                                                                                        >  2:1655504/1‑149 (MQ=255)
                                                                    tCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCg                                                                                    >  2:1024808/1‑151 (MQ=255)
                                                                     cGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGt                                                                                   >  2:1421061/1‑151 (MQ=255)
                                                                     cGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGt                                                                                   >  1:1690080/1‑151 (MQ=255)
                                                                       ttccgaaaaCGGTC‑GTAATGTGAAGAAAAAGCCGTAA‑GGGAGAAAACCCGCGTT‑GCC‑GGCCCGCTTTTAAA‑TGTGCTGTCCCCCCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGt                                                                                   <  2:267809/143‑1 (MQ=255)
                                                                         aCGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTc                                                                               >  1:1859939/1‑151 (MQ=255)
                                                                                 ggTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTg                                                                       >  2:66963/1‑151 (MQ=255)
                                                                                  gTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCtt                                                                        >  1:634990/1‑149 (MQ=255)
                                                                                  gTC‑GAAATGTGCAGGATAGGCCTAAG‑CGGAGAGAACCCGCGTG‑GCT‑GGCCCGCTTTTCAA‑TGTGCTGTCCCCCCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCtt                                                                        <  2:634991/149‑1 (MQ=255)
                                                                                    c‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTc                                                                    >  1:2143511/1‑151 (MQ=255)
                                                                                    c‑GTAATGTGCAGGAAAAGCCGTAA‑CGGAGAGAACCCGCGTT‑GCC‑GGTCCGTTTTTCAA‑TGTGCTTTCCCCCCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGtt                                                                     <  2:2633665/150‑1 (MQ=255)
                                                                                     ggTAATGTCAAGAATAGGCCGAAG‑CGGAAAAACCCCGCGTC‑GCC‑GGCCCGTTGTTAAT‑GGTGCTCTCCCCCCCCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTc                                                                    <  2:2200745/150‑1 (MQ=255)
                                                                                     aggagtgtgCAGGATAA‑CCATAAGGGGAGACACCCCGCGTT‑GCC‑GCTCCTGTGTTCAT‑TGGTCTCCCCCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGtt                                                                     <  2:265215/145‑1 (MQ=255)
                                                                                      gTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAg                                                                                      >  2:2624791/1‑132 (MQ=255)
                                                                                      gTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTc                                                                    >  2:2202464/1‑150 (MQ=255)
                                                                                          tgtgCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTg                                                                       >  1:1789069/1‑143 (MQ=255)
                                                                                          tgtgCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCt                                                               >  2:2370043/1‑151 (MQ=255)
                                                                                           gtgCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTg                                                              >  2:114617/1‑151 (MQ=255)
                                                                                              cAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTc                                                                               >  2:512974/1‑131 (MQ=255)
                                                                                               aGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGc                                                          >  1:1091445/1‑151 (MQ=255)
                                                                                                  aaaaGCCGTAC‑CGGAAAGAACCCGCGTT‑GCC‑GTTCCGTTTTTAAT‑TGTGCTGTCCCCCCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAg                                                       <  2:1614629/149‑1 (MQ=255)
                                                                                                    aaGCCGTAA‑GGGAGAAAACCCTCTTT‑GCG‑GGTCCGCTGTTCAT‑TGTGCTGTCCCCCCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGaa                                                     <  2:1423845/151‑1 (MQ=255)
                                                                                                    aaGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGa                                                      >  1:1068376/1‑150 (MQ=255)
                                                                                                      gCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGt                                                   >  1:1906770/1‑151 (MQ=255)
                                                                                                        ccaaaagggAGAAAACCCGTGTT‑GCC‑GTCCCGTTTTTCAT‑TGTGCTGTCCCCCCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTc                                                  <  2:2252518/144‑1 (MQ=255)
                                                                                                          tAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTcac                                                >  2:1129618/1‑150 (MQ=255)
                                                                                                          tAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTcac                                                >  1:2425587/1‑150 (MQ=255)
                                                                                                            acgggAGAGACCCCGCGTG‑CCC‑GGCCCGCTGTCAAG‑TGTCCGGGCCCCCCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAg                                              <  1:247776/148‑1 (MQ=255)
                                                                                                            a‑CGGAGAAAACCCG‑TTT‑GCCGGGTCCGCTTTGAAT‑TGTGCTCCCCCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAgg                                             <  1:114617/151‑1 (MQ=255)
                                                                                                               ggagaaaccccGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTCCCCCCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGaa                                           <  2:2523953/142‑1 (MQ=255)
                                                                                                                   agaACCCGGGTT‑GCC‑GGCCGGCTGTTAAA‑TGTGCTGTCCCCCCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAatca                                       <  2:1071542/151‑1 (MQ=255)
                                                                                                                    gaATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAatcat                                      >  2:1828731/1‑151 (MQ=255)
                                                                                                                      aacccgtttcgCC‑GGCCCGTTTTTCAT‑TGTCCTCTCCCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAatcatc                                     <  1:1421056/141‑1 (MQ=255)
                                                                                                                      aTCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGcc                                                         >  1:1019355/1‑130 (MQ=255)
                                                                                                                      aTCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAatcatc                                     >  2:194091/1‑150 (MQ=255)
                                                                                                                        ccGCTTT‑CCG‑GGTCCGCT‑TTCAATTGTCTTGTCCCCCCCCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAAc                                  <  1:66963/151‑1 (MQ=255)
                                                                                                                        ccGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAAc                                  >  2:267847/1‑151 (MQ=255)
                                                                                                                         cgcgTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAAcc                                 >  2:1032834/1‑151 (MQ=255)
                                                                                                                           cgcgtggcgtcgccGTTGTTTAT‑TTTGCTGTCCCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAAcc                                 <  1:1805100/139‑1 (MQ=255)
                                                                                                                             tt‑GCC‑GGCCCGTTTTTCAA‑TGTGCTGTCCCCCCCCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGc                               <  2:2425599/149‑1 (MQ=255)
                                                                                                                              t‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTg                             >  1:2094113/1‑150 (MQ=255)
                                                                                                                                gCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGAc                           >  1:2282111/1‑151 (MQ=255)
                                                                                                                                  c‑GGTCCGCTTTTAAA‑TGTGCTGTCCCCCCCCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGACaa                         <  2:2143519/151‑1 (MQ=255)
                                                                                                                                    gccccgtttttCAT‑TGTTCTGTCCCCTCCCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGACAAg                        <  1:2076126/142‑1 (MQ=255)
                                                                                                                                     gccccttGTTAAA‑TGTGCTTTCCCCTCCCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGACAAGc                       <  2:2094121/145‑1 (MQ=255)
                                                                                                                                      tCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGACAAGc                       >  1:114286/1‑150 (MQ=255)
                                                                                                                                       ccgttttccAA‑TGTGCTGTCCCCCCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGACAAGCa                      <  1:2010529/142‑1 (MQ=255)
                                                                                                                                         tttgtaaaa‑TGTCCTGCCCCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGACAAGCATc                    <  2:181197/143‑1 (MQ=255)
                                                                                                                                           tGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGACAAGCATCCTg                 >  1:2403572/1‑151 (MQ=255)
                                                                                                                                             ttCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCTGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGACAAGCATCCTGt                >  1:2600625/1‑150 (MQ=255)
                                                                                                                                               cAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGTGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGACAAGCATCCTGTAGt             >  1:2648212/1‑151 (MQ=255)
                                                                                                                                                aa‑TGTCCTGCCCCCCCCCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGACAAGCATCCTGTAGTc            <  1:1828726/151‑1 (MQ=255)
                                                                                                                                                      gCTGTCCCCCCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGACAAGCATCCTGTAGTCGCTg        <  2:1690085/150‑1 (MQ=255)
                                                                                                                                                           aCCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGACAAGCATCCTGTAGTCGCTGGATATg  >  1:1605755/1‑151 (MQ=255)
                                                                                                                                                           aCCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGACAAGCATCCTGTAGTCGCTGGATATg  >  2:2431350/1‑151 (MQ=255)
                                                                                                                                                           aCCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGACAAGCATCCTGTAGTCGCTGGATATg  >  2:2176799/1‑151 (MQ=255)
                                                                                                                                                           |                                                                                                                                                      
GATGTTTAATACCTGCAAGATGAGGATTGCGCCCCAGCCGCACCGGGCTTAGCGCCAACGTAATCCCCTCGTTACGCAAACGGTC‑GTAATGTGCAGGATAAGCCGTAA‑CGGAGAGAATCCGCGTT‑GCC‑GGTCCGCTGTTCAA‑TGTGCTGTACCCTCGCCCGCCACTACCGCGACTGATCACGACTTTCAGCACACCATTTTGCTGTTCTGCTGCCAGCGTTTTCATCTCTTGTTCAAGCTGAGGCCAGAAGTCACAGGAAATCATCAACCGCTGACAAGCATCCTGTAGTCGCTGGATATG  >  DH1/2726016‑2726316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑