Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,782,1580TC40.3% 216.5 / 28.8 139noncoding (72/88 nt)ECDH1_RS13860tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (74/9);  new base C (0/56);  total (74/65)
Fisher's exact test for biased strand distribution p-value = 9.33e-30
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

TTTTCAAAAAGTTTGTTGACCTCAGGTCATGATTTCCCTAAATTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGCCAGTTGGACAGACCGTGAACGCAGTG  >  DH1/2782009‑2782303
                                                                                                                                                        |                                                                                                                                                 
ttttCAAAAAGTTTGTTGACCTCAGGTCATGATTTCCCTAAATTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAAt                                                                                                                                                   <  2:1631393/150‑1 (MQ=255)
ttttCAAAAAGTTTGTTGACCTCAGGTCATGATTTCCCTAAATTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATc                                                                                                                                                  >  2:104122/1‑151 (MQ=255)
    cAAAAAGTTTGTTGACCTCAGGTCATGATTTCCCTAAATTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATccccc                                                                                                                                              >  2:1265935/1‑151 (MQ=255)
      aaaaGTTTGTTGACCTCAGGTCATGATTTCCCTAAATTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATccccccc                                                                                                                                            <  1:1900065/151‑1 (MQ=255)
           tttGTTGACCTCAGGTCATGATTTCCCTAAATTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCAcc                                                                                                                                       >  1:2551941/1‑151 (MQ=255)
             tgttgACCTCAGGTCATGATTTCCCTAAATTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCg                                                                                                                                      <  2:396695/150‑1 (MQ=255)
              gttgACCTCAGGTCATGATTTCCCTAAATTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGc                                                                                                                                     <  1:1603883/150‑1 (MQ=255)
                tgACCTCAGGTCATGATTTCCCTAAATTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCat                                                                                                                                  >  1:2571949/1‑151 (MQ=255)
                  aCCTCAGGTCATGATTTCCCTAAATTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCatat                                                                                                                                <  2:2035669/151‑1 (MQ=255)
                   ccTCAGGTCATGTTTCCCCAAAATGAGCGCCCGTTCCAGCAAGACAGGAACGAAATTTT‑GGTGGGGGGTCCGGGTGGCGGAAGGACCACGCCTGGAAAGTGTTTATA‑CGGCAAC‑GTAGGGGGGGTTCAACCCCCCCCCTCACCGCCATAtt                                                                                                                               <  1:407803/151‑1 (MQ=18)
                        ggTCATGATTTCCCTAAATTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTaa                                                                                                                            >  2:1834873/1‑149 (MQ=255)
                            aTGTTTTCCAAAAATTGGCGCCCGTCCAAACAAGACAGAAACGAAATTTG‑GGTGAGGTGCCGGGGTGGTGGAAGGAGCCCCCCTGAAAAGTGTGTATA‑CGCAAAC‑GTATGGGGGGTTCGACCCCCCCCCTCACCGCCATATTTAAagaaga                                                                                                                      <  1:523952/151‑1 (MQ=9)
                            aTGATTTCCCTAAATTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAaaga                                                                                                                         >  1:151395/1‑148 (MQ=255)
                            aTGATTTCCCAAAATTAGCGCCCGTCCCAGAAAGAAAGGAACGACATTTT‑GGTGGGGTGTCCGAGTGGCGGAAGGAGCACGCCTGAAAAGTGTGTATA‑CGGAAAG‑GTAGCGGGGGTTCGAACCCCCCCCTCACCGCCATATTTAAagaaga                                                                                                                      <  1:740027/151‑1 (MQ=17)
                              gtttccccAAAATTAGCGCCCGTTCCAGAAAGACAGGAACGAAATTTG‑GGTGGGGTGTCCGAGGGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAACCCCCCCCTCACCGCCATATTTAAAGAAGAGc                                                                                                                    <  2:1478234/146‑1 (MQ=17)
                                tttCCCTAAATTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTc                                                                                                                  >  2:1082992/1‑151 (MQ=255)
                                 tcccaaaaaTGAGCGCCCTTCCAAAAAAAAAAGGACCGAAATTTT‑GGGGGGGTGTCGGGGTGGCAGAAGGAGCCCCCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATGGGGGGTTCGAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTc                                                                                                                  <  1:1061014/144‑1 (MQ=2)
                                   cccTAAATTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGt                                                                                                                >  2:2195026/1‑150 (MQ=255)
                                     cTAAATTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACg                                                                                                             >  2:2390133/1‑151 (MQ=255)
                                      aaaaTTAGCCCCCGTCCCAGAAAGACAGGAACGAAATTTG‑GGGGGGGTGTCCGGGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATGGGGGGTTCGAACCCCCCCCTAACCGCCATATTTAAAGAAGAGCTCGTACGa                                                                                                            <  2:150864/150‑1 (MQ=14)
                                       cccgacccgcCCGTCCCAGCAAGACAGAAACGAAATTTT‑GGTGAGGTGTCGGAGTGGCTGAAGGAGCCCGCCTGAAAAGTGTGTATA‑GGGCAAC‑GTATGGGGGTTTCGACCCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACg                                                                                                             <  2:454347/142‑1 (MQ=14)
                                        aaTTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGaa                                                                                                           >  1:1254986/1‑150 (MQ=255)
                                            ggcgcccttccaaaaaaGACAGGAACGAAATTTT‑GGTGAGGTGTCCGGGGGGCTGAAGGAGCACGCTTGGAAAGTGTGTATA‑CGGCAAC‑GTAGGGGGGTTTCGAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTAc                                                                                                      <  2:2615569/136‑1 (MQ=14)
                                            ggcgcCCGTTCCAGAAAGAAAGGAACGAAAATTG‑GGTGGGGTGCCCGAGTGGCGGAAGGAGCACCCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATGGGGGTTTCAAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTAc                                                                                                      <  1:2195017/150‑1 (MQ=14)
                                            ggcccccTTTCAAGAAAGACGGGAACGGTAAGTT‑GGGGGGGTGTCCGGGGGGCGGAAGAAGCACGCCTGGAAAGTGTGTATC‑CGGCAAC‑GTGTCGGGGTTTGGATTCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTAc                                                                                                      <  1:694972/147‑1 (MQ=2)
                                            aGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTa                                                                                                                             >  2:2263956/1‑128 (MQ=255)
                                            aGCGCCCGTCCCAGAAAGACAGGAACGAAATTTT‑GGTGGGGTGCCCGAGTGGCTGAAGGAGCCCGCCTGAAAAGTGTGTATA‑CGGCAAC‑GTAGCGGGGGTTCGAACCCCCCCCTCACCGCCATATTTa                                                                                                                             <  1:2263947/128‑1 (MQ=14)
                                             gcgcCCGTTCCAGAAAGACAGGAACGAAAATTT‑GGGGAGGTGCCCGAGTGGCTGAGGGAGCACGCCTGAAAGGTGTTTATA‑CGGCAAC‑GTTTGGGGGTTTCAAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACg                                                                                                     <  2:1490010/151‑1 (MQ=17)
                                               gcCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑TGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACg                                                                                                             >  1:454348/1‑141 (MQ=255)
                                                 ccGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCt                                                                                                 >  2:1294843/1‑151 (MQ=255)
                                                 ccGTCCCAGAAAGACAGGAACGAAAATTG‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGAAAAGTGTGTATA‑CGGCAAC‑GTATGGGGGGTTCGAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCt                                                                                                 <  2:151395/151‑1 (MQ=14)
                                                  aatctaagaaaGACAGGAACAAAATTTG‑GGGGAGGTGTCCGAGTGGCTGAAGGAGCACGCGTGGAAGGTGTGTATA‑CGGCAAG‑GTATGGGGGGTTCAAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCtt                                                                                                <  1:768166/142‑1 (MQ=14)
                                                    tcccAGAAAGACAGGAACAAAAATTG‑GGGGAGGTGTCGGAGTGGCGGAAGGAGCACCCCTGAAAAGTGTGTATA‑CGCAAAG‑GTATGGGGGGTTCAAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCtt                                                                                                <  1:1265933/147‑1 (MQ=14)
                                                      ccAGAAAGACAGGAACGACATTTT‑GGTGGGGTGCCGGGGGGGCTGAAGGACCCCGCCTGAAAAGTGTGTATA‑CGGAAAC‑GTAGGGGGGGTTCGAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCtttttt                                                                                            <  1:104122/151‑1 (MQ=14)
                                                       cAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCtt                                                                                                >  2:768167/1‑146 (MQ=255)
                                                        agaaaGACAGGAACGAAATTTG‑GGGGAGGTGTCCGGGTGGCTGAAGGCGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATGGGGGTTTCGAACCCCCCCCTCTCCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCtttttttt                                                                                          <  1:2195871/148‑1 (MQ=14)
                                                         aaaaaaCAGAAACGAAAATTT‑GGTGGGGTGCCGGAGTGGCTGAAGGAGCACCCCTGGAAAGTGTGTATA‑CGGCAAC‑GTAGGGGGGTTTCAAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTc                                                                                         <  1:1834868/146‑1 (MQ=11)
                                                          aaaGAAAGGAACGAAATTTG‑GGTGGGGTGCCCGAGTGGCGGAGGGAGCACGCCGGAAAGGTGTGTATG‑CGGAAAC‑GTATCGGGGTTTCGAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCg                                                                                        <  2:2385644/150‑1 (MQ=14)
                                                           aaGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCtttttttttcg                                                                                       >  1:1348364/1‑148 (MQ=255)
                                                            aGACAGGAACGAAATTTG‑GGTGAGGTGCCCGGGTGGCGGAAGGAGCCCGCCTGGAAAGTGTTTATA‑CGGCAAC‑TTATGGGGGTTTCGAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGta                                                                                      <  2:2571964/151‑1 (MQ=14)
                                                             gACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGtat                                                                                     >  2:724094/1‑151 (MQ=255)
                                                             aaaaGGAACGACATTTT‑GGTGAGGTGTCCGGGGGGATGAAGGAGCACCCCTGAAAAGTGTGTATA‑CGCAAAC‑GTAGCGGGGGTCCAACCCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGtat                                                                                     <  1:144500/148‑1 (MQ=14)
                                                              aCAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGtat                                                                                     >  1:1419161/1‑150 (MQ=255)
                                                              aCAGAAACGAAAATTT‑GGTGGGGTGCCCGGGTGGCGGAAGAGGCACGCCGGGAAAGTGTGTATA‑CGGAAAC‑GTATGGGGGGTTCAAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGtata                                                                                    <  2:1513940/151‑1 (MQ=14)
                                                                aGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATAtt                                                                                  >  2:1620002/1‑151 (MQ=255)
                                                                aGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATAtt                                                                                  >  1:379774/1‑151 (MQ=255)
                                                                aGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATAtt                                                                                  >  1:1603675/1‑151 (MQ=255)
                                                                aGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATAtt                                                                                  >  2:267281/1‑151 (MQ=255)
                                                                  gAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGc                                                                                >  1:439792/1‑151 (MQ=255)
                                                                   aaCGAAATTTT‑GGTGAGGTGTCCGGGTGGCTAAAGGAGCCCCCCTGGAAAGTGTGTATA‑CGGAAAC‑TTATCGGGGGTTCGAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGca                                                                               <  2:1419166/151‑1 (MQ=14)
                                                                      aaCATTTG‑GGTGGGGTGCCGGGGGGGCGGAAGGACCACGCCTGGAAAGTGTTTATA‑CGGAAAC‑TTATGGGGGGTTCAAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGcacac                                                                            <  1:724093/150‑1 (MQ=14)
                                                                       aaaaTTTGGGTGAGGTGCCGGGGTGGCTGAAGGACCCCCCCTGAAAAGTGTGTATA‑CGGCAAC‑GTAGGGGGGGTTCAAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGcacac                                                                            <  1:267282/149‑1 (MQ=14)
                                                                         aaTTTGGGTGAGGTGTCGGGGTGGCGAAGGGAGCCCGCCTGAAAAGTGTTTATA‑CGGAAAC‑GTATGGGGGGTACAACCCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCacacac                                                                          <  1:2589889/151‑1 (MQ=14)
                                                                         aaTTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACAcc                                                                         >  2:338911/1‑151 (MQ=255)
                                                                           ttt‑GGTGAGGTGTCGGGGTGGGTGAAGGACCACGCCTGAAAAGTGTGTATA‑CGGCACC‑GTATGGGGGGTTCAAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCg                                                                        <  1:338912/150‑1 (MQ=14)
                                                                           ttt‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCgg                                                                       >  1:2517578/1‑151 (MQ=255)
                                                                            tt‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCggg                                                                      >  2:961859/1‑151 (MQ=255)
                                                                                ggggggTGCCCGGGTGGCTAAAGGGCCCCGCCTGAAAAGTGTGTATA‑CGGCAAG‑GTTTGGGGGGTTCAAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCgggggg                                                                   <  1:2390123/147‑1 (MQ=17)
                                                                                gTGGGGTGTCCGGGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGACAAC‑GTATGGGGGTTTCAAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCgggggg                                                                   <  1:84952/151‑1 (MQ=17)
                                                                                gTGGGGTGTCCGGGTGGCTAAGGGAGCCCGCCGGGAAAGTGTGTATA‑CGCAAAC‑GTATCGGGGGTTCGAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCgggggg                                                                   <  2:869343/151‑1 (MQ=17)
                                                                                   aGGTGCCCGGGTGGCTGAAGGACCAGGCC‑GGAAAG‑GTTTATATCCGCAACTGTAGGGGGGGTTAAAACCCCCCCCCCGCCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATg                                                                <  2:379773/151‑1 (MQ=9)
                                                                                          cgggTGGCGGAGGGCGCCCGCCGGAAAGGTGTTAATA‑CGGAAAC‑GTAGGGGGGGTTCAAACCCCCCCCCCTCCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGcc                                                         <  1:961858/148‑1 (MQ=14)
                                                                                           gAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGccc                                                        >  2:725991/1‑151 (MQ=255)
                                                                                            aGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGcccc                                                       >  2:789701/1‑151 (MQ=255)
                                                                                             gTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCg                                                      >  2:2450934/1‑151 (MQ=255)
                                                                                              tGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCCTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCg                                                      >  2:1034574/1‑150 (MQ=255)
                                                                                                gCTAAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATGGGGGTTTCAAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCggg                                                                      <  1:2350672/132‑1 (MQ=17)
                                                                                                 cTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCg                                                  >  2:527818/1‑151 (MQ=255)
                                                                                                 cTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCg                                                  >  1:2048932/1‑151 (MQ=255)
                                                                                                  tGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCgg                                                 >  1:962634/1‑151 (MQ=255)
                                                                                                   gAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGTGGGGATGAGAAGCCCCGACCggg                                                >  1:1438883/1‑151 (MQ=255)
                                                                                                     aGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGt                                              >  1:2418523/1‑151 (MQ=255)
                                                                                                     aGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGt                                              >  2:1215136/1‑151 (MQ=255)
                                                                                                         gCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCg                                           >  2:160122/1‑150 (MQ=255)
                                                                                                             gCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAAc                                      >  2:211020/1‑151 (MQ=255)
                                                                                                                 ggAAAGTGTGTATA‑CGGAAAC‑GTAGGGGGGGTTCGAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTg                                    <  1:1261973/149‑1 (MQ=255)
                                                                                                                   aagttttgtAAA‑CGGAGAC‑TTGTCGGTGGTTCGACCCCCCCCCCCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGa                                <  1:1512333/145‑1 (MQ=11)
                                                                                                                   aaaGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCg                                 >  2:2291023/1‑150 (MQ=255)
                                                                                                                   aaaGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCg                                 >  2:1393518/1‑150 (MQ=255)
                                                                                                                     aGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGAc                               >  1:1244561/1‑150 (MQ=255)
                                                                                                                      gtgtgtATA‑CGGAAAC‑GTATGGGGGGTGCAACCCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAg                             <  1:2020632/151‑1 (MQ=14)
                                                                                                                        gtttaaa‑CGGCAAC‑GTATCGGGGTTTCAAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGc                            <  2:2517590/144‑1 (MQ=14)
                                                                                                                        gtgtATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGcc                           >  1:1216578/1‑151 (MQ=255)
                                                                                                                            ata‑CGGAAAC‑TTATGGGGGTTTCGAACCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGCCAGtt                       <  2:439791/151‑1 (MQ=255)
                                                                                                                              a‑CGGCAAC‑GTATGGGGGTTTCAAACCCCCCCCTACCCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGCCAGTTg                      <  1:2291014/150‑1 (MQ=14)
                                                                                                                              a‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGCCAGTTgg                     >  2:847993/1‑151 (MQ=255)
                                                                                                                               acgaaacc‑GTATGGGGGGTTCGACCCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGCCAGTTgg                     <  1:1393513/144‑1 (MQ=11)
                                                                                                                                cctgaagtttATCGGGGTTTCGATCCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGCCAGTTgg                     <  2:2048940/142‑1 (MQ=11)
                                                                                                                                 ggCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCg                                 >  1:22335/1‑137 (MQ=255)
                                                                                                                                 ggCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGCCAGTTGGAc                   >  2:2553809/1‑151 (MQ=255)
                                                                                                                                 ggCAAC‑GTAGGGGGGGTTCGACCCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCg                                 <  2:22335/137‑1 (MQ=255)
                                                                                                                                    aaC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGCCAGTTGGAc                   >  1:2461069/1‑148 (MQ=255)
                                                                                                                                        gggcgacgggTTCCAATCCCCCCTTTCCCGCCATATTTAAACAAGAGCTCGTACGAAAGTACGGGCCTTTTCCTCGTATATTGCACACTCCGGGGGGATGAGAAGCCCCGTCCGGGGTTCGACAACTGGCGACAGCCAGTTGGACAGACCg             <  1:977582/144‑1 (MQ=11)
                                                                                                                                         tATCTGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAATAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATAATGTACACAGCGTGGGGATGAGAAGCCGCGTCCAGGGTTCGACAACCGGGGACAGCCAGTTGTAGAGTCCgg            >  1:166523/1‑150 (MQ=11)
                                                                                                                                         tATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGCCAGTTGGACAGACCGt            >  2:1083595/1‑151 (MQ=39)
                                                                                                                                          aTCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGCCAGTTGGACAGACCGTg           >  1:821429/1‑151 (MQ=38)
                                                                                                                                                   tCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGCCAGTTGGACAGACCGTGAACGCAGTg  >  2:2311029/1‑151 (MQ=31)
                                                                                                                                                    cGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGCCAGTTGGACAGAcc              >  1:747241/1‑138 (MQ=31)
                                                                                                                                                    cGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGCCAGTTGGACAGACCGTGAACGCAGTg  >  2:1952177/1‑150 (MQ=31)
                                                                                                                                                     gAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGcc                           >  1:1208585/1‑124 (MQ=11)
                                                                                                                                                        |                                                                                                                                                 
TTTTCAAAAAGTTTGTTGACCTCAGGTCATGATTTCCCTAAATTAGCGCCCGTTCCAGCAAGACAGGAACGACAATTT‑GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATA‑CGGCAAC‑GTATCGGGGGTTCGAATCCCCCCCTCACCGCCATATTTAAAGAAGAGCTCGTACGAAAGTACGGGCTTTTTTTTCGTATATTGCACACACCGGGGGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGCCAGTTGGACAGACCGTGAACGCAGTG  >  DH1/2782009‑2782303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑