Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,281,5430AC38.8% 313.5 / 31.9 152V288G (GTG→GGG) cusAcation efflux system protein CusA
Reads supporting (aligned to +/- strand):  ref base A (79/14);  new base C (0/59);  total (79/73)
Fisher's exact test for biased strand distribution p-value = 4.52e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

AGGTTGTCGATGGCGCGGTCAATGAGCTGGCTGCGATCGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCTTTTAAAACGATGTGATTAAAGTCGTCGAGCGTT  >  DH1/3281393‑3281692
                                                                                                                                                       |                                                                                                                                                     
aGGTTGTCGATGGCGCGGTCAATGAGCTGGCTGCGATCGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATca‑cca                                                                                                                                                       <  2:972232/151‑1 (MQ=255)
  gTTGTCGATGGCGCGGTCAATGAGCTGGCTGCGATCGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCa‑ccacc                                                                                                                                                     <  2:1282428/151‑1 (MQ=255)
    tGTCGATGGCGCGGTCAATGAGCTGGCTGCGATCGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCAccc                                                                                                                                                    <  2:556172/150‑1 (MQ=255)
     gTCGATGGCGCGGTCAATGAGCTGGCTGCGATCGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCAcccc                                                                                                                                                   <  1:1266350/150‑1 (MQ=255)
       cGATGGCGCGGTCAATGAGCTGGCTGCGATCGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACcccgcc                                                                                                                                                >  2:795098/1‑151 (MQ=255)
        gATGGCGCGGTCAATGAGCTGGCTGCGATCGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACcccgccc                                                                                                                                               >  1:275445/1‑151 (MQ=255)
             cgcgGTCAATGAGCTGGCTGCGATCGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCAc                                                                                                                                          >  2:1122820/1‑151 (MQ=255)
               cgGTCAATGAGCTGGCTGCGATCGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCActt                                                                                                                                        <  1:229026/151‑1 (MQ=255)
                    aaTGAGCTGGCTGCGATCGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCActtcgcct                                                                                                                                   >  1:1210686/1‑151 (MQ=255)
                     aTGAGCTGGCTGCGATCGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCActtcgcctt                                                                                                                                  >  1:661553/1‑151 (MQ=255)
                      tGAGCTGGCTGCGATCGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCActtcgccttc                                                                                                                                 >  2:1193126/1‑151 (MQ=255)
                       gAGCTGGCTGCGATCGTATTTTGTAACTATCTCCACGCCTTCCGGCAGCCTCCTTTTCAGCGTTTCCAGTTTGTCCTCCACGGCGGCGATCCCTTCTCGGGCGTTTTTGCGGGATCGCAGGATCA‑CCCCCCCGCCCGCCActtcgccttc                                                                                                                                 <  2:275444/150‑1 (MQ=255)
                         gctggctgCGATCGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACttcgccttcgcc                                                                                                                              >  1:1818582/1‑151 (MQ=255)
                             gctgCGATCGTATGTTGTACCTATCTCCACGCCTTCGGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGTGGGCGATCACTTCTCGGGCGTTTTGGCCGGATCGCAGGATCA‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGttt                                                                                                                          <  2:661554/151‑1 (MQ=255)
                               tgCGATCGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGttt                                                                                                                          >  1:749039/1‑149 (MQ=255)
                                gCGATCGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGCTTCCAGTTTGTCCTTAACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCCCCGTTTAGt                                                                                                                       >  1:2013815/1‑151 (MQ=255)
                                     cGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGc                                                                                                                  >  2:2306863/1‑151 (MQ=255)
                                      gTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGc                                                                                                                  >  1:2056837/1‑150 (MQ=255)
                                      gTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGc                                                                                                                  >  1:308709/1‑150 (MQ=255)
                                       tATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCa                                                                                                                 >  1:500371/1‑150 (MQ=255)
                                       tATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCa                                                                                                                 >  2:1047668/1‑150 (MQ=255)
                                        aTGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAAt                                                                                                               >  2:1672526/1‑151 (MQ=255)
                                             gtAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGc                                                                                                                  >  1:2210940/1‑143 (MQ=255)
                                              tAACTATCTCCACGCCTTCCGGCAGACTATTTTTCAGCGTTTCCAGTTTGTCCTTCCCGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGcccc                                                                                                          <  2:2025129/150‑1 (MQ=255)
                                                  tATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGgcgc                                                                                                     >  2:584599/1‑151 (MQ=255)
                                                       ccACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCAtctc                                                                                                >  1:608841/1‑151 (MQ=255)
                                                        cccGCCTTCCGGCAGACTACTTTTCAGCTTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCTTTTTTCCCGGATCGCAGGATCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTcc                                                                                               <  2:500371/149‑1 (MQ=255)
                                                         aCGCCTTCGGGCAGACTATTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCC‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCg                                                                                              <  1:2306854/151‑1 (MQ=255)
                                                             cTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGcc                                                                                          >  1:898447/1‑151 (MQ=255)
                                                                ccGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAAt                                                                                       >  1:2178686/1‑151 (MQ=255)
                                                                   gCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTg                                                                                    >  1:1214074/1‑151 (MQ=255)
                                                                    cAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTgg                                                                                   >  1:801308/1‑151 (MQ=255)
                                                                    cAGACTACTTTTCAGCGTTTCAAGTTTGTCCTTCACGGCGCCGACCACTCCTCGGGCGTTTTTGCCGGACCGAAGGATCA‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGGTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTgg                                                                                   <  2:898448/151‑1 (MQ=255)
                                                                      gACTACTTTTCAGCTTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCTTTTTTGCCGGATCGCAGGATCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCa                                                                                         <  2:190636/143‑1 (MQ=255)
                                                                      gACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCa                                                                                         >  1:190637/1‑143 (MQ=255)
                                                                       actcctTTTCAGCTTTTCCAGTTTGTCCTTCCCGGCGGCAATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGAcc                                                                                <  2:1355792/151‑1 (MQ=255)
                                                                        tttctTTCCAGCGTTTCCAGTTTGTCCTTCGCGGCGGCGATCACTTCTCGGGCGTTTTGGCGGGATCGCGGGATCA‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCt                                                                                     <  2:551669/142‑1 (MQ=255)
                                                                        ctactTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGATTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCt                                                                                     >  1:551668/1‑145 (MQ=255)
                                                                         tactTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCtt                                                                              >  2:1962574/1‑151 (MQ=255)
                                                                          tctTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCCCTTCTCGGGCGTTTTTGCCGGATCGCAGGATCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCtt                                                                              <  2:801309/149‑1 (MQ=255)
                                                                          actTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTc                                                                             >  1:1369082/1‑151 (MQ=255)
                                                                           ctTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCg                                                                            >  1:293534/1‑151 (MQ=255)
                                                                             tttCAGCTTTTCCAGTTTGCCCTTCCCGGCGGCGATCACTTCTGGGGCGTTTTTGCCGGATCGCAGGATCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTc                                                                             <  2:2178694/148‑1 (MQ=255)
                                                                                cAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCTGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAAc                                                                        >  1:2622056/1‑150 (MQ=255)
                                                                                 aGCGTTTCGAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGAGCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTTGTTCGGCAATGCCCCCCCGCATCTCCGGGCCAATCTGGACCTTCGCAAcc                                                                       >  1:2136029/1‑149 (MQ=255)
                                                                                     tttCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATcgcg                                                                  >  1:2345904/1‑151 (MQ=255)
                                                                                         cAGTTTGTCCTTCACGGCGGCGATCCCTTCTCGGGCTTTTTTGCCGGATCGCAGGATCC‑CCCCCCCCCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGa                                                              <  1:1962568/151‑1 (MQ=255)
                                                                                          aGTTTTTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGACCGCAGAATCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGAt                                                             <  2:293533/151‑1 (MQ=255)
                                                                                           gTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGAt                                                             >  2:2247261/1‑150 (MQ=255)
                                                                                           gTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGAt                                                             >  2:157632/1‑150 (MQ=255)
                                                                                            tttGTCCTTCACGGCGGCGATCCCTTCGCGGGCGTTTTGGCCGGATCGCAGGACCA‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCg                                                                            <  1:958790/134‑1 (MQ=255)
                                                                                            tttGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATa                                                            >  2:2630152/1‑150 (MQ=255)
                                                                                              ggTCCTTCACGGCGGCGATCACTTCTCGGGCTTTTTTGCCGGATCGCAGGATCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATaaa                                                          <  2:1166332/149‑1 (MQ=255)
                                                                                               gTCCTTCCCGGCGGCGCTCCCTTCTCGGGGGTTTTTGCCGGACCGCAGGATCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGAt                                                             <  1:581560/146‑1 (MQ=255)
                                                                                                 ccTTCCCGGGGGCGATCCCTTCCCGGGCTTTTTTGCCGGATCGCAGGATCA‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACgg                                                       <  2:608842/150‑1 (MQ=255)
                                                                                                 ccTTCACGGCGGCGATCCCTTCTCGGGCTTTTTTGCCGGATCGCAGGATCA‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACggg                                                      <  2:1818587/151‑1 (MQ=255)
                                                                                                   ttCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGc                                                     >  2:1978674/1‑150 (MQ=255)
                                                                                                    ccACGGCGGCGATCTCTTCCCGGGTGTTTTGGCCGGAGCGCATGACCA‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCAc                                                   <  2:219310/150‑1 (MQ=255)
                                                                                                    acACGGCGGCGCTCACTTCTCGGGCGTTTTGGCGGGATCGCAGGACCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCa                                                    <  1:621434/149‑1 (MQ=255)
                                                                                                     cagggcggcGATCACTTCTCGGGCGTTTTTCCCGGATCGCAGGACCC‑CCCCCCCGCCCGCCACTTCGCCTTCACCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACg                                                  <  1:2630136/148‑1 (MQ=255)
                                                                                                     cACGGCGGCGTTCACTTCTGGGGCTTTTTTGCCTGATCGCAGGATCA‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATACCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACg                                                  <  2:878502/151‑1 (MQ=255)
                                                                                                     cACGGCGGCGATCCCTTCTCGGGCTTTTTTGCCGGATCGCAGG‑TCACCCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACg                                                  <  1:2277694/151‑1 (MQ=255)
                                                                                                     cACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCAc                                                   <  2:2345914/150‑1 (MQ=255)
                                                                                                     cACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACg                                                  >  2:5173/1‑151 (MQ=255)
                                                                                                     cACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACg                                                  >  1:736919/1‑151 (MQ=255)
                                                                                                       cggcggCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGcc                                                <  1:2510461/151‑1 (MQ=255)
                                                                                                       ccatccgatccattcctcGGCAGTTTTTGCCGGTTCGCAGGCCCC‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGcc                                                <  1:776705/136‑1 (MQ=255)
                                                                                                        ggcggcGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGcc                                                >  1:1243944/1‑150 (MQ=255)
                                                                                                           ggcgATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCAttt                                            >  2:802254/1‑151 (MQ=255)
                                                                                                            gcaaTCCCTTCTCGGGCGTTTTTGCCGGATCGCGGGATCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCAtttt                                           <  2:668112/148‑1 (MQ=255)
                                                                                                             cgATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGcc                                                >  2:776706/1‑145 (MQ=255)
                                                                                                               aTCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCAc                                        >  2:243769/1‑151 (MQ=255)
                                                                                                                  ccTTCTCGGGCTTTTTTGCCGGATCCCGGAACCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTgg                                     <  1:1115033/150‑1 (MQ=255)
                                                                                                                  aCTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTgg                                     >  2:342733/1‑151 (MQ=255)
                                                                                                                  aCTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTgg                                     >  2:1115034/1‑151 (MQ=255)
                                                                                                                   cTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACt                                       <  1:1030271/148‑1 (MQ=255)
                                                                                                                   cTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACt                                       >  2:1030272/1‑148 (MQ=255)
                                                                                                                    ttCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCt                                   >  2:2140135/1‑151 (MQ=255)
                                                                                                                       tcGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCtttt                                >  1:1426243/1‑151 (MQ=255)
                                                                                                                       attctcGTTTTCGCGGGACCGAAGGATCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCtttt                                <  2:1550298/146‑1 (MQ=255)
                                                                                                                        cGGGCTTTTTTGCCGGATCGAAGGACCA‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCttt                                 <  1:157632/149‑1 (MQ=255)
                                                                                                                        cGGGCGTTTTTGCCGGATCGCAGGATCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCttt                                 <  2:1214076/149‑1 (MQ=255)
                                                                                                                        cGGGCGTTTTTGCCGGATCGCAGGACCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCtttt                                <  2:736920/150‑1 (MQ=255)
                                                                                                                        cGGGCGTTTTTGCCGGATCGCAGGACCA‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCtt                                  <  1:1029961/148‑1 (MQ=255)
                                                                                                                         gggCTTTTTTGCCGGATCGCAGGATCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCTTTTaa                              <  2:1426248/151‑1 (MQ=255)
                                                                                                                             gTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCTTTTAAAACg                          >  2:1992054/1‑151 (MQ=255)
                                                                                                                              tctttGCCGGATCGTAGGATCA‑CCACACCGCCCTCTACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCATTCTGGACCTTCGCAACATCGCGCAGATAAACGGCCACGCCATTTTCACTGGCTTTTAAAACg                          >  1:2485538/3‑150 (MQ=255)
                                                                                                                               ttttGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCTTTTAAAACGAt                        >  1:1648496/1‑151 (MQ=255)
                                                                                                                                tttCCCGGTCCGCAGGACCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCTTTTAAAACGAt                        <  1:675446/150‑1 (MQ=255)
                                                                                                                                 ttGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCTTTTAAAACGAt                        >  1:1112700/1‑149 (MQ=255)
                                                                                                                                  tGCCGAATCGCAGGACCC‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCTTTTAAAACGAtgt                      <  1:1472447/150‑1 (MQ=255)
                                                                                                                                    ccGGACCGCAGAGTCC‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCTTTTAAAACGATGTGAt                   <  2:2136037/151‑1 (MQ=255)
                                                                                                                                        cgaccAGGTTCC‑CCACCCGCCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCGACTCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCTTTTAAAACGATGTGATTaaa               <  2:2485550/147‑1 (MQ=255)
                                                                                                                                        aTCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCTTTTAAAACGATGTGATTaa                >  1:876683/1‑150 (MQ=255)
                                                                                                                                          cGCAGGACCA‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCTTTTAAAACGATGTGATTAAAgt             <  1:2157678/151‑1 (MQ=255)
                                                                                                                                           gCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCTTTTAAAACGATGTGATTAAAgtc            >  1:1673295/1‑151 (MQ=255)
                                                                                                                                            gagaaTCA‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGTCATTTTCACTGGCTTTTAAAACGATGTGATTAAAgtcg           <  1:802253/147‑1 (MQ=255)
                                                                                                                                             aGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCTTTTAAAACGATGTGATTAAAgtcgt          >  2:2633788/1‑151 (MQ=255)
                                                                                                                                              ggATCA‑CCCCCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCTTTTAAAACGATGTGATTAAAgtcgtc         <  1:5173/151‑1 (MQ=255)
                                                                                                                                                      caccCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCTTTTAAAACGATGTGATTAAAGTCGTCGAGCGtt  >  2:1415804/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
AGGTTGTCGATGGCGCGGTCAATGAGCTGGCTGCGATCGTATGTTGTAACTATCTCCACGCCTTCCGGCAGACTACTTTTCAGCGTTTCCAGTTTGTCCTTCACGGCGGCGATCACTTCTCGGGCGTTTTTGCCGGATCGCAGGATCA‑CCACCCCGCCCGCCACTTCGCCTTCGCCGTTTAGTTCGGCAATGCCCCGGCGCATCTCCGGGCCAATCTGGACCTTCGCAACATCGCGCAGATAAACGGGCACGCCATTTTCACTGGCTTTTAAAACGATGTGATTAAAGTCGTCGAGCGTT  >  DH1/3281393‑3281692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑