Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,513,0650GT46.4% 101.3 / 69.7 110L267F (TTG→TTT) cynRtranscriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (13/46);  new base T (51/0);  total (64/46)
Fisher's exact test for biased strand distribution p-value = 1.87e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 118 total aligned reads displayed.

GTCATTGAGGCGAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTATC  >  DH1/3512916‑3513213
                                                                                                                                                               |                                                                                                                                                       
  cATTGAGGCGAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTttttttt                                                                                                                                                      >  2:1037067/1‑150 (MQ=255)
   aTTGAGGCGAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTtttttttc                                                                                                                                                     >  1:2135731/1‑149 (MQ=255)
    ttGAGGCGAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTtcg                                                                                                                                                    >  2:1999511/1‑151 (MQ=255)
     tGAGGCGAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTgcgg                                                                                                                                                   >  2:1281498/1‑151 (MQ=255)
     ggaggaaaaCTAAATTAGCGGGTTTGGGGAGCT‑TTTCCCCCCCCTTCCCTTTCCCACTTTTTT‑CCAAG‑CACCTATCCCCAC‑AAAACAGGA‑GGGTTT‑AAATT‑TTTTTT‑CTCTC‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTgcg                                                                                                                                                    <  1:1065859/143‑1 (MQ=255)
      gAGGCGAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGCGGGTTttgttcggg                                                                                                                                                   >  1:443430/1‑147 (MQ=255)
       aGGCGAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTgcggcg                                                                                                                                                 >  2:752502/1‑151 (MQ=255)
         gCGAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTtggcggg                                                                                                                                               >  1:1864045/1‑150 (MQ=255)
           gAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGTCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTGCGGCGGaa                                                                                                                                              >  2:2202768/1‑150 (MQ=255)
           gAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTGcggcggga                                                                                                                                              >  1:2300649/1‑148 (MQ=255)
                caattggggggtttCTGGAGTTTATCCGCCCCCCTCCCCTTC‑CCATTTTTTT‑ACCAC‑CAGCGACTCCCAA‑AAAACATG‑CGGGTTT‑TAA‑T‑TTTTTTCCTCTT‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAAt                                                                                                                                          <  1:1570071/137‑1 (MQ=255)
                cAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGgaaaaaa                                                                                                                                         >  1:91351/1‑148 (MQ=255)
                  aTTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTGCGGCGGgaaaaaaact                                                                                                                                      >  1:1215977/1‑146 (MQ=255)
                  aTTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTGCGGCGGGAAAATAGCt                                                                                                                                      >  2:2437834/1‑151 (MQ=255)
                   ttAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTGCGGCGGGAAAATaagtt                                                                                                                                     >  1:297268/1‑147 (MQ=255)
                    tggcggGGTTCGGGGGCTGTTCCCCCCCTCTCCCCTTC‑CCACTTTGTA‑ACCAG‑CAGCGATCCCCAA‑AAAACAGGAGGGTTTA‑AAA‑T‑TTTTTT‑CTTTT‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTg                                                                                                                                     <  2:1864050/148‑1 (MQ=255)
                    tAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTCGGCGGgaaaaaaaatgg                                                                                                                                    >  1:1832042/1‑144 (MQ=255)
                     aGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTGCGGGGGGAAAATAGCTggg                                                                                                                                   >  2:1111249/1‑150 (MQ=255)
                      gcgcGTTTCGGGAGTTGATCCCCCCCCCTCCCCTTC‑CCATTTTTTC‑CCCAG‑CAGCGATCCCCAC‑AAAACAGGAGGGGTTA‑AAA‑T‑TTTTTT‑CTCTC‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCa                                                                                                                                  <  2:236573/151‑1 (MQ=255)
                       cgggtttggggAGCTGATCCCCCGCACTCCCCTTC‑CCCTTTTGTC‑CCCAG‑CGGCGATCCCCAC‑AAAAAAGGGGGGTTTA‑AAA‑G‑TTTTTC‑CTCTG‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAg                                                                                                                                 <  1:1111248/142‑1 (MQ=255)
                        gcgGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTGCGGCGGGAAAAAAGCTGGc                                                                                                                                   >  2:1809132/1‑148 (MQ=255)
                         cgGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTgc                                                                                                                                                     >  1:665450/1‑129 (MQ=255)
                           gTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAGCGGC‑GGTTTTTTTTCGGCGGGAAAAAAACTTGCAGACa                                                                                                                              >  1:780817/1‑150 (MQ=255)
                           gTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTCGGCGGGAAAAAAGCCTGGAGACa                                                                                                                              >  1:592610/1‑150 (MQ=255)
                           gTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTGCGGCGGAAAAAAAGATGGGAGGCa                                                                                                                              >  2:2638422/1‑150 (MQ=255)
                               tGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTTGGCGGGAAAAAAACTGGCAGaaaacccc                                                                                                                         >  1:581988/1‑144 (MQ=255)
                                   ccgttccccccgcCCTCCCTTTT‑CCATTTTTTC‑CCCAG‑CAGCGATCCCCAA‑AAAAAAGGGCGGTATA‑AAA‑T‑TTTTTT‑CTCTC‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCcgc‑g                                                                                                                    <  1:667957/142‑1 (MQ=255)
                                    gcttatccccccctctCCCTTTCCCAATTTTTT‑CCCAG‑CAGCGATCCCCAC‑AAAACAGGGCGGTTTA‑AAA‑T‑TTTTTT‑CTCTC‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCcgc‑g                                                                                                                    <  2:592611/136‑1 (MQ=255)
                                          cgccgcACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTCGGCGGGAAAAAAACTTGCCGACAACCCCCCC‑G‑aggtcgg                                                                                                            >  2:667958/1‑145 (MQ=255)
                                           gccgcACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTGTGGCGGGAAAAAAACTGGCCGACAGCCGCCCC‑G‑CAGGCCtt                                                                                                           >  1:745038/1‑151 (MQ=255)
                                            cccccctccccTTC‑CCACTTTGTC‑ACCAG‑CAGCAATCCCCAC‑AAAACAGGAGGGGTTA‑AAA‑T‑TTTTTT‑CTCTT‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCAttt                                                                                                          <  2:1743156/143‑1 (MQ=255)
                                             cccccctCCCCTT‑CCAATTTTTTAACCAG‑CAGCGAGCCCCAC‑AAAAAATGAGGGTTTT‑AAT‑T‑TTTTTT‑CTCTG‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCAttt                                                                                                          <  1:2557128/145‑1 (MQ=255)
                                              gcACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTCGGCGGGAAAAAAACTGGCAGACAGCCGCCCC‑G‑GAAGCAttttt                                                                                                        >  2:1666621/1‑151 (MQ=255)
                                              cccctccccTTC‑CCACTTTTTA‑CCCAG‑CACGGATCCCCAC‑AAAACATG‑CGGTTTT‑AAA‑GTTTTTTT‑CTCTC‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCAtttt                                                                                                         <  2:335929/144‑1 (MQ=255)
                                               ccctccccTTC‑CCAC‑TTTTT‑ACCAGCCGGCAATCCCCAC‑ACACCAGGA‑GGGCTT‑TAA‑A‑GTTTTT‑CCCTTCCCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTg                                                                                                       <  2:1082339/146‑1 (MQ=255)
                                               cACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTGGGCGGGAAAAAAACTGGGCGGAAGCCGCCCC‑C‑GAGGCAtttttt                                                                                                       >  2:2574426/1‑150 (MQ=255)
                                                aCTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTCGGCGGGAAAATAGCCGGCAGACAGGCGCCGC‑G‑GAGGGGtttttt                                                                                                       >  1:335930/1‑149 (MQ=255)
                                                        tt‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTGCGGCGGGAAAAAAACTGGCAGACAGCCGCCGC‑G‑AAAGCATTTTTTCACATGGc                                                                                               >  2:340693/1‑150 (MQ=255)
                                                          tcccattttgtccccAG‑CAGCGATCCCCAC‑AAAACATGGGGGGTTT‑AAA‑T‑TTTTTT‑CTCGC‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGc                                                                                               <  1:340694/137‑1 (MQ=25)
                                                           cccatttGTT‑CCCGG‑CAGCGTTCCCCCC‑ACAAAATG‑TGGGCTTAAAA‑G‑TTTTTT‑CTCTG‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGtt                                                                                            <  2:2271536/146‑1 (MQ=25)
                                                            ccattttttcaCCAG‑CAGGGA‑TCCCACAACAACGTGGGGGGATA‑AAA‑G‑TTTTTT‑CTCTT‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGtt                                                                                            <  2:1215979/141‑1 (MQ=25)
                                                            cacaTTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTGCGGCGGGAAAAAAAATGGCAGACAGCCGCCGC‑G‑AAAGCATTTTTTCACATGGCGGTg                                                                                           >  2:1872554/1‑151 (MQ=255)
                                                              tttttttcaCCAG‑CGCCTATTCCCAC‑AAAACATGAGGGTTTT‑AAA‑T‑TTTTTC‑CTCTC‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTg                                                                                           <  1:1984954/142‑1 (MQ=25)
                                                               aTTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTCGGCGGGAAAATATCTGGCAGACAACCGCCCC‑G‑GAAGCATTTTTTTACAAGGGGtt                                                                                            >  1:1059194/1‑147 (MQ=255)
                                                                 ttttccccAG‑CCGCGAGCCCCAC‑AAAACATGGGGGGTTA‑AAA‑T‑TTTTTT‑CTCTC‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGa                                                                                         <  2:1184144/143‑1 (MQ=25)
                                                                    t‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGGTTTTTTGGGGGGGGAAAATAGCTGGGCGAAAGCCGCCCC‑C‑GACGGATTTTTTCACCTTGAGTTGGATTAAtt                                                                                   >  2:139588/1‑150 (MQ=255)
                                                                     caCCAG‑CGGCGTTCCCCAC‑AAAACAGGAGGGGTTA‑AAA‑T‑TTTTTT‑CTTTC‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATg                                                                                   <  2:2300658/150‑1 (MQ=25)
                                                                      cccAG‑CAGCATTCCCCAC‑AAAACAGGAGGGGTTA‑AAA‑T‑TTTTTT‑CTCTT‑CCCCCGCCACTACTGGAGAGAGCGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATg                                                                                   <  2:780818/149‑1 (MQ=25)
                                                                                  tccccAC‑ACAAAAGGACGGGTTA‑AAA‑G‑TTTTTT‑CTCTT‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGttgttg                                                                        <  2:1056047/147‑1 (MQ=37)
                                                                                  tccccAC‑AAAACATGGGGGGTTT‑AAT‑T‑TTTTTT‑CTCTC‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGttgttg                                                                        <  1:2437822/147‑1 (MQ=25)
                                                                                      cac‑acaACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTTGGGCGGGAAAATAGCTGGCAGACAGCCGCCGCGG‑AAGGC‑TTTTTTCACATGGCGGTGGGTAAAAGCGCGGGTGTTGggggg                                                                   >  2:405574/1‑150 (MQ=255)
                                                                                          acaacaTGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTTGGCGGGAAAAAAACCGGGAGGAAACCGCCCC‑G‑GAGGGCTTTTTTTACAATGCGGTGGATAAAAGCGCGGGTGTTTGCGGgaa                                                                 >  1:1098416/1‑150 (MQ=255)
                                                                                                   cGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTCGGCGGGAAAAAAACCGGGAGACAGCCCCCCC‑G‑GAGGGATTTTTTCACAAGGCGGTGGGTAAAATCGCGGGTGTTTGCGGGAAAGAAATAC‑gg                                                      >  1:65772/1‑151 (MQ=255)
                                                                                                   cGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTGCGGCGGGAAAAAAGCTGGGAGACAACCGCCGC‑G‑AAAGCATTTTTTTACATGGCGTTGGGAAAAAGGGCGGGTGTTTGCGGGAAAGAATAAC‑gg                                                      >  2:1082956/1‑151 (MQ=255)
                                                                                                    gggCTT‑AAATG‑TTTTTT‑CTCTC‑CCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑gg                                                      <  2:2557061/151‑1 (MQ=25)
                                                                                                    gggCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTGCGGCGGGAAAATAGATGGCAGACAGCCGCCGC‑G‑AAAGCATTTTTTCACATGGCGTTGGGTAAATGCGCgggt                                                                            >  2:530996/1‑127 (MQ=255)
                                                                                                        tt‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTCGGCGGGAAAAAAAGTGGGAGACAGCCGCCCC‑C‑AAAGGCTTTTTTCACAATGTGTTTGGTAAAAGGCCGGGTGTTGGCGGGAATTaaaaaatcaga                                                    >  1:93354/1‑140 (MQ=255)
                                                                                                        tt‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGTGGCGGGAAAAAAACTGGCCGACAGTCCCCGC‑C‑GAGGCATTTTTTCCCATGGCGTTGGGTAAATGCCGGGTTGTTTGGGGGAAAGAAACAC‑CGTAggc                                                 >  1:2604148/1‑149 (MQ=255)
                                                                                                           tcctttcttttt‑CTCTG‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGc                                                  <  2:1188636/141‑1 (MQ=255)
                                                                                                           aaa‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTCGGCGGGAAAAAAACTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTTCACATGGCGTTGGGTAAATGCGCGGGTGTTGGGGGGAATGAATCAC‑GGTAGCCGc                                               >  2:1566039/1‑151 (MQ=21)
                                                                                                            aa‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTTGGCGGGAAAAAAACTGGCCGAAAACCGCCGC‑G‑GAGGCATTTTTTTACAAGGCGGTGGATAAAAGCGCGGGTGTTGGGGGGAATAAATCAC‑GGTAGCCGcc                                              >  1:1148112/1‑150 (MQ=255)
                                                                                                            aa‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTTTGGCGGGAAAAAAACTGGCAGACAGCCCCCGC‑G‑AAGGCATTTTTTCACATGGCGGTGGGTAAAAGCGCGGGTGTTGGCGGGAATTAAACAC‑GGGAGCCGCt                                              >  2:2388201/1‑151 (MQ=255)
                                                                                                               g‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGGAAAATAGCTGGCAGACAGCCGCCGC‑G‑GAAGCATTTTTTCACATGGCGTTGGGTAAATGCGCGGGTGTTGGCGGGAAAGAATCAC‑GGTAGCCGCTg                                             >  2:425498/1‑150 (MQ=255)
                                                                                                                aatttttttctttgcccccGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCt                                              <  2:1098417/136‑1 (MQ=255)
                                                                                                                 cTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTGCGGCGGGAAAAAAGATGGCAGACAGCCCCCCC‑G‑AAAGGAATTTTGCACAAGGCGGTGGGTAAAAGCGGGGGTTTTGGGGGAAATTAATCAC‑GGGAGGCGCCGa                                            >  2:2068334/1‑150 (MQ=255)
                                                                                                                   ttttttccgttatgccGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGa                                            <  1:139588/136‑1 (MQ=255)
                                                                                                                   aTTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTCGGCGGGAAAAAAACTGGCAGAAAACCCCCCC‑G‑AAGGCGGTTTTTCACATTGGGTTGGGTAAAATCGCGGGTGTTGGGGGGAAAGAATAAC‑GGGAgaagaaccgg                                          >  1:1188634/1‑141 (MQ=255)
                                                                                                                    ttttcttctccccccGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGac                                           <  2:581989/139‑1 (MQ=255)
                                                                                                                     tt‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGGTTTTTTAGGGCGGGAAAAAAGCTGGGCGACAGCCGCCGG‑GCAAGGAATTTTTG‑ACATGGGGTTGGGTAAAAGGCCGGGTGTTGGGGGGAAAAAATCAA‑AGGAGCCGCTGacac                                         >  2:1142443/1‑149 (MQ=255)
                                                                                                                         ccttgcccccGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAgg                                      <  2:65772/144‑1 (MQ=255)
                                                                                                                          tctccccccGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGt                                     <  2:312474/145‑1 (MQ=255)
                                                                                                                          ctT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGGGGGGGGAAAACAACTGGCCGACAACCGCCGC‑G‑GAGGGATTTTTTCACCAGGGGGTGGGTAAAAGGGCGGGTGTTGGGGGGGAAGAATTAC‑GGGGGGCGCTGGCACAggggt                                   >  1:467275/1‑148 (MQ=255)
                                                                                                                           ccggCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑A‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTc                                    <  1:2651783/147‑1 (MQ=255)
                                                                                                                             cccgccGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTg                                  <  1:1648228/146‑1 (MQ=255)
                                                                                                                              gCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGGAAAAAAACTGGCAGACAGCCGCCCC‑G‑AAAGCATTTTTTCACATGGCGGTGGGTAAATGCGCGGGTGTTGGCGGGAATGAAACAC‑CGTAGCCGCCGACACAGGGCTGCg                                >  2:1049063/1‑151 (MQ=255)
                                                                                                                               ccccGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTGGGGGGGAAAAAAGCCTGCCGAAAGACGGCCC‑G‑GAGGAATTTTTTCACAAAGCGTTGGGTAAAAGGGCGGGTTTTTGCGGGAAAGAGATAC‑GGGAGACGCTggaaaaaggcttcgg                               >  1:1518392/1‑137 (MQ=255)
                                                                                                                               ccccGCCACTACTGGAGAGAACGGC‑GGTTTTTTTGCGGCGGGAAAAAAACTGGCCGAAAGCCGCCCC‑G‑AAGGGAATTTTTTACAAGGCGTTGGATAAAAGCGCGGGTGTTGGCGGGAAAGAATCAC‑GGGAGCCGCCGACACAGGGCTGCgg                               >  2:303562/1‑151 (MQ=255)
                                                                                                                                cccGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTCGGCGGGAAAAAAACTGGGAGACAGCCCCCCC‑G‑CAGGCCTTTTTTTACCTGGGGGTTGGGAAAAGCGCGGGTGTTTGGGGGAAAGAAACAC‑CGGAGGCGCCGACACAAGGCTggggg                              >  1:1028907/1‑150 (MQ=255)
                                                                                                                                cccGCCACTACTGGAGAGAACGGC‑GGTTTTTTTTCGGCGGGAAAAAAACTCGGCGAAAGGCGCCCC‑G‑CAGGCCTTTTTTTACATGGGGGTGGGTAAATGCGCGGGGGGTGGGGGGGAAGAATCAC‑GGGGGGCGCTGAcacaaccttgtcgc                              >  1:1168569/1‑141 (MQ=255)
                                                                                                                                cccGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGGGGAAAAATAACTGGGAGACAGGCGCCCC‑G‑GAGGAATTTTTGTCCATGGCGGTGGATAAAAACGCGGGTGTTGGGGGGAAAGAATCACGGGTAG‑CGCTGACACAAGTCTGTggg                              >  2:2539666/1‑150 (MQ=255)
                                                                                                                                cccGCCACTACTGGAGAGAACGGC‑GGATTTTTTGCGGCGGAAAAAAAACTCGCAAACAACCGCCGC‑G‑AAAGCCTTTTTTCACATAGGGGTGGGTTAAAGCGCGGGTTTTTGCGGGAAAGAAACCC‑GGTTAGCGCTGAAACAGGCCTGccgg                              >  2:1728220/1‑148 (MQ=255)
                                                                                                                                 ccGCCACTACTGGAGAGAACGGC‑GGTTTTTTTGCGGCGGGAAAAAAGCTTGCAGACAGCCGCCGC‑G‑GAGGCCTTTTTTTCCATGGCGGTTGGTAAATGCGCGGGTGTTGGGGGGAAAGAATAAC‑GGTAGGCGCCGaggtcgggagagcggc                             >  1:1644484/1‑136 (MQ=255)
                                                                                                                                 ccGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTg                                             <  2:1644489/135‑1 (MQ=255)
                                                                                                                                        tactGGAGAGAACGGC‑GGTTTTTTTGCGGCGGGAAAATAGCTTGCAGACCGCCGCCGC‑G‑GAGGGATTTTTTCACACGGCGGTGGGTAAATGGGCGGGTGTTGGCGGGAATGAAACAC‑GGGAGCCGCTGACACCGGGCTGCgggatcggga                       >  2:240574/1‑142 (MQ=255)
                                                                                                                                        tactGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACCGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCg                                <  1:240575/141‑1 (MQ=255)
                                                                                                                                         actGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATAGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTg                      <  1:1609278/150‑1 (MQ=255)
                                                                                                                                           tGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATc                                                           <  2:2018925/112‑1 (MQ=255)
                                                                                                                                            ggAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCt                   <  2:1148113/150‑1 (MQ=255)
                                                                                                                                             gagagaACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGa                 <  2:467275/151‑1 (MQ=255)
                                                                                                                                              agagaACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTg                      <  2:321198/145‑1 (MQ=255)
                                                                                                                                                   aCGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCt           <  1:1728215/151‑1 (MQ=255)
                                                                                                                                                    cggc‑ggTTTTGTTGCGGCGGAAAAATAGCTGGCAGACCGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGGTGTTGGCGGAAATGAATTAC‑GGTAGCCGCTGACACAGGGCTGCGGCTGGGATTGCCTGATgagatt          >  2:1043603/1‑146 (MQ=18)
                                                                                                                                                    cggc‑ggTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATg               <  1:1043602/146‑1 (MQ=255)
                                                                                                                                                     ggc‑ggTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGGTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTAc         >  1:1789692/1‑151 (MQ=255)
                                                                                                                                                      gc‑ggTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACg        <  1:425499/151‑1 (MQ=255)
                                                                                                                                                       c‑ggTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGc       >  1:2210305/1‑151 (MQ=255)
                                                                                                                                                            tttGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTAt   >  2:615410/1‑151 (MQ=255)
                                                                                                                                                              tgttgCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCtt     >  2:1764045/1‑147 (MQ=255)
                                                                                                                                                              tgttgCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCtt     <  1:1764040/147‑1 (MQ=255)
                                                                                                                                                              tgttgCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTATc  >  1:183508/1‑150 (MQ=255)
                                                                                                                                                               |                                                                                                                                                       
GTCATTGAGGCGAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAG‑CAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑G‑CTATTT‑CTCTT‑GCCCCGCCACTACTGGAGAGAACGGC‑GGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCACATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTATC  >  DH1/3512916‑3513213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑