Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,513,0750AG39.1% 159.1 / 37.6 115K271E (AAA→GAA) cynRtranscriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (15/55);  new base G (45/0);  total (60/55)
Fisher's exact test for biased strand distribution p-value = 2.96e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

GAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGT  >  DH1/3512927‑3513225
                                                                                                                                                          |                                                                                                                                                          
gAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGTCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGCGGaa                                                                                                                                                           >  2:2202768/1‑150 (MQ=255)
     caattggggggtttCTGGAGTTTATCCGCCCCCCTCCCCTTC‑CCATTTTTTT‑ACCACCAGCGACTCCCAA‑AAAACATG‑CGGGTTT‑TAA‑TTTTTTTCCTCTTCCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAAt                                                                                                                                                       <  1:1570071/137‑1 (MQ=255)
     cAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGgaaaaaa                                                                                                                                                      >  1:91351/1‑148 (MQ=255)
       aTTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGCGGgaaaaaaact                                                                                                                                                   >  1:1215977/1‑146 (MQ=255)
       aTTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGCGGGAAAATAGCt                                                                                                                                                   >  2:2437834/1‑151 (MQ=255)
         tggcggGGTTCGGGGGCTGTTCCCCCCCTCTCCCCTTC‑CCACTTTGTA‑ACCAGCAGCGATCCCCAA‑AAAACAGGAGGGTTTA‑AAA‑TTTTTTT‑CTTTTCCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTg                                                                                                                                                  <  2:1864050/148‑1 (MQ=255)
          aGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGGGGGAAAATAGCTggg                                                                                                                                                >  2:1111249/1‑150 (MQ=255)
           gcgcGTTTCGGGAGTTGATCCCCCCCCCTCCCCTTC‑CCATTTTTTC‑CCCAGCAGCGATCCCCAC‑AAAACAGGAGGGGTTA‑AAA‑TTTTTTT‑CTCTCGCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCa                                                                                                                                               <  2:236573/151‑1 (MQ=255)
            cgggtttggggAGCTGATCCCCCGCACTCCCCTTC‑CCCTTTTGTC‑CCCAGCGGCGATCCCCAC‑AAAAAAGGGGGGTTTA‑AAA‑GTTTTTC‑CTCTGCCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAg                                                                                                                                              <  1:1111248/142‑1 (MQ=255)
             gcgGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGCGGGAAAAAAGCTGGc                                                                                                                                                >  2:1809132/1‑148 (MQ=255)
                gTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAGCGGCGGTTTTTTTTCGGCGGGAAAAAAACTTGCAGACa                                                                                                                                           >  1:780817/1‑150 (MQ=255)
                gTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTTCGGCGGGAAAAAAGCCTGGAGACa                                                                                                                                           >  1:592610/1‑150 (MQ=255)
                gTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGCGGAAAAAAAGATGGGAGGCa                                                                                                                                           >  2:2638422/1‑150 (MQ=255)
                    tGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTTTGGCGGGAAAAAAACTGGCAGaaaacccc                                                                                                                                      >  1:581988/1‑144 (MQ=255)
                    tGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTTCGGCGGGAAAAAAACTGGCAGACAgccgc                                                                                                                                      >  1:610264/1‑151 (MQ=255)
                         gcttatccccccctctCCCTTTCCCAATTTTTT‑CCCAGCAGCGATCCCCAC‑AAAACAGGGCGGTTTA‑AAA‑TTTTTTT‑CTCTCCCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCcgc‑g                                                                                                                                 <  2:592611/136‑1 (MQ=255)
                          tGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAAAAACTGGCAGaaaaaaacggg                                                                                                                                   >  2:272043/1‑138 (MQ=255)
                               cgccgcACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTTCGGCGGGAAAAAAACTTGCCGACAACCCCCCC‑G‑aggtcgg                                                                                                                         >  2:667958/1‑145 (MQ=255)
                                gccgcACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTGTGGCGGGAAAAAAACTGGCCGACAGCCGCCCC‑G‑CAGGCCtt                                                                                                                        >  1:745038/1‑151 (MQ=255)
                                  cccccctCCCCTT‑CCAATTTTTTAACCAGCAGCGAGCCCCAC‑AAAAAATGAGGGTTTT‑AAT‑TTTTTTT‑CTCTGCCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCAttt                                                                                                                       <  1:2557128/145‑1 (MQ=255)
                                   gcACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTTCGGCGGGAAAAAAACTGGCAGACAGCCGCCCC‑G‑GAAGCAttttt                                                                                                                     >  2:1666621/1‑151 (MQ=255)
                                    cACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTTGGGCGGGAAAAAAACTGGGCGGAAGCCGCCCC‑C‑GAGGCAtttttt                                                                                                                    >  2:2574426/1‑150 (MQ=255)
                                     aCTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTTCGGCGGGAAAATAGCCGGCAGACAGGCGCCGC‑G‑GAGGGGtttttt                                                                                                                    >  1:335930/1‑149 (MQ=255)
                                             tt‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGCGGGAAAAAAACTGGCAGACAGCCGCCGC‑G‑AAAGCATTTTTTCAC‑ATGGc                                                                                                           >  2:340693/1‑150 (MQ=255)
                                               tcccattttgtccccAGCAGCGATCCCCAC‑AAAACATGGGGGGTTT‑AAA‑TTTTTTT‑CTCGCCCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGc                                                                                                           <  1:340694/137‑1 (MQ=25)
                                                cccatttGTT‑CCCGGCAGCGTTCCCCCC‑ACAAAATG‑TGGGCTTAAAA‑GTTTTTT‑CTCTGGCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGtt                                                                                                        <  2:2271536/146‑1 (MQ=25)
                                                 ccattttttcaCCAGCAGGGA‑TCCCACAACAACGTGGGGGGATA‑AAA‑GTTTTTT‑CTCTTCCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGtt                                                                                                        <  2:1215979/141‑1 (MQ=25)
                                                 cacaTTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGCGGGAAAAAAAATGGCAGACAGCCGCCGC‑G‑AAAGCATTTTTTCAC‑ATGGCGGTg                                                                                                       >  2:1872554/1‑151 (MQ=255)
                                                 atgtattttccccAGCAGCGATCCCCAC‑AAAACAGGGCGGTTTA‑AAA‑TTTTTTT‑CTTTGCCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGtt                                                                                                        <  2:1059195/140‑1 (MQ=21)
                                                   tttttttcaCCAGCGCCTATTCCCAC‑AAAACATGAGGGTTTT‑AAA‑TTTTTTC‑CTCTCCCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTg                                                                                                       <  1:1984954/142‑1 (MQ=25)
                                                    aTTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTTCGGCGGGAAAATATCTGGCAGACAACCGCCCC‑G‑GAAGCATTTTTTTAC‑AAGGGGtt                                                                                                        >  1:1059194/1‑147 (MQ=255)
                                                      ttttccccAGCCGCGAGCCCCAC‑AAAACATGGGGGGTTA‑AAA‑TTTTTTT‑CTCTCCCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGa                                                                                                     <  2:1184144/143‑1 (MQ=25)
                                                         t‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGGTTTTTTGGGGGGGGAAAATAGCTGGGCGAAAGCCGCCCC‑C‑GACGGATTTTTTCAC‑CTTGAGTTGGATTAAtt                                                                                               >  2:139588/1‑150 (MQ=255)
                                                          caCCAGCGGCGTTCCCCAC‑AAAACAGGAGGGGTTA‑AAA‑TTTTTTT‑CTTTCCCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATg                                                                                               <  2:2300658/150‑1 (MQ=25)
                                                           cccAGCAGCATTCCCCAC‑AAAACAGGAGGGGTTA‑AAA‑TTTTTTT‑CTCTTCCCCCGCCACTACTGGAGAGAGCGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATg                                                                                               <  2:780818/149‑1 (MQ=25)
                                                              agcagcGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGCGGGAAAAAAACCGGCAGAAAGCCGCCCC‑G‑GAGGGCTTTTTTCAC‑AAGGGGGTGGGTAAAATcgcg                                                                                           >  1:920801/1‑151 (MQ=255)
                                                                      tccccAC‑ACAAAAGGACGGGTTA‑AAA‑GTTTTTT‑CTCTTCCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGttgttg                                                                                    <  2:1056047/147‑1 (MQ=37)
                                                                      tccccAC‑AAAACATGGGGGGTTT‑AAT‑TTTTTTT‑CTCTCCCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGttgttg                                                                                    <  1:2437822/147‑1 (MQ=25)
                                                                          cac‑acaACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTTGGGCGGGAAAATAGCTGGCAGACAGCCGCCGCGG‑AAGGC‑TTTTTTCAC‑ATGGCGGTGGGTAAAAGCGCGGGTGTTGggggg                                                                               >  2:405574/1‑150 (MQ=255)
                                                                              acaacaTGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTTTGGCGGGAAAAAAACCGGGAGGAAACCGCCCC‑G‑GAGGGCTTTTTTTAC‑AATGCGGTGGATAAAAGCGCGGGTGTTTGCGGgaa                                                                             >  1:1098416/1‑150 (MQ=255)
                                                                                aacaTGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTTCGGCGGGAAAAAAGCTGGCAGACAGCCGCCCC‑G‑AAGGCATTTTTTTAC‑AAAACGGTGGGTAAAAGCGCGGGTGTTGGCGGGAATg                                                                           >  2:1609283/1‑150 (MQ=255)
                                                                                       cGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTTCGGCGGGAAAAAAACCGGGAGACAGCCCCCCC‑G‑GAGGGATTTTTTCAC‑AAGGCGGTGGGTAAAATCGCGGGTGTTTGCGGGAAAGAAATAC‑gg                                                                  >  1:65772/1‑151 (MQ=255)
                                                                                       cGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGCGGGAAAAAAGCTGGGAGACAACCGCCGC‑G‑AAAGCATTTTTTTAC‑ATGGCGTTGGGAAAAAGGGCGGGTGTTTGCGGGAAAGAATAAC‑gg                                                                  >  2:1082956/1‑151 (MQ=255)
                                                                                        gggCTT‑AAATGTTTTTT‑CTCTCCCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑gg                                                                  <  2:2557061/151‑1 (MQ=25)
                                                                                        gggCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGCGGGAAAATAGATGGCAGACAGCCGCCGC‑G‑AAAGCATTTTTTCAC‑ATGGCGTTGGGTAAATGCGCgggt                                                                                        >  2:530996/1‑127 (MQ=255)
                                                                                          gCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGCGGGAAAAAAACTGGCAGACAGCCGCCCC‑G‑AAAGCATTTTTTCAC‑ATGGCGTTGGGTAAAAGCGCGGGTGTTGGCGGGAATGAATCAC‑GGt                                                                 >  2:2196789/1‑149 (MQ=25)
                                                                                            tt‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGTGGCGGGAAAAAAACTGGCCGACAGTCCCCGC‑C‑GAGGCATTTTTTCCC‑ATGGCGTTGGGTAAATGCCGGGTTGTTTGGGGGAAAGAAACAC‑CGTAggc                                                             >  1:2604148/1‑149 (MQ=255)
                                                                                              tcctttcttttt‑CTCTGGCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGc                                                              <  2:1188636/141‑1 (MQ=255)
                                                                                                aa‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTTTGGCGGGAAAAAAACTGGCCGAAAACCGCCGC‑G‑GAGGCATTTTTTTAC‑AAGGCGGTGGATAAAAGCGCGGGTGTTGGGGGGAATAAATCAC‑GGTAGCCGcc                                                          >  1:1148112/1‑150 (MQ=255)
                                                                                                aa‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTTTGGCGGGAAAAAAACTGGCAGACAGCCCCCGC‑G‑AAGGCATTTTTTCAC‑ATGGCGGTGGGTAAAAGCGCGGGTGTTGGCGGGAATTAAACAC‑GGGAGCCGCt                                                          >  2:2388201/1‑151 (MQ=255)
                                                                                                  aatttttttctttgcccccGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCt                                                          <  2:1098417/136‑1 (MQ=255)
                                                                                                   gCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGGAAAATAGCTGGCAGACAGCCGCCGC‑G‑GAAGCATTTTTTCAC‑ATGGCGTTGGGTAAATGCGCGGGTGTTGGCGGGAAAGAATCAC‑GGTAGCCGCTg                                                         >  2:425498/1‑150 (MQ=255)
                                                                                                    cTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGCGGGAAAAAAGATGGCAGACAGCCCCCCC‑G‑AAAGGAATTTTGCAC‑AAGGCGGTGGGTAAAAGCGGGGGTTTTGGGGGAAATTAATCAC‑GGGAGGCGCCGa                                                        >  2:2068334/1‑150 (MQ=255)
                                                                                                     ttttttccgttatgccGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGa                                                        <  1:139588/136‑1 (MQ=255)
                                                                                                      ttttcttctccccccGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGac                                                       <  2:581989/139‑1 (MQ=255)
                                                                                                      aTTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTTCGGCGGGAAAAAAACTGGCAGAAAACCCCCCC‑G‑AAGGCGGTTTTTCAC‑ATTGGGTTGGGTAAAATCGCGGGTGTTGGGGGGAAAGAATAAC‑GGGAgaagaaccgg                                                      >  1:1188634/1‑141 (MQ=255)
                                                                                                        tt‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGGTTTTTTAGGGCGGGAAAAAAGCTGGGCGACAGCCGCCGG‑GCAAGGAATTTTTG‑AC‑ATGGGGTTGGGTAAAAGGCCGGGTGTTGGGGGGAAAAAATCAA‑AGGAGCCGCTGacac                                                     >  2:1142443/1‑149 (MQ=255)
                                                                                                        tt‑CTCTGCCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGAcaca                                                    <  1:1566034/150‑1 (MQ=255)
                                                                                                            tctccccccGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGt                                                 <  2:312474/145‑1 (MQ=255)
                                                                                                             ctTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGGGGGGGGAAAACAACTGGCCGACAACCGCCGC‑G‑GAGGGATTTTTTCAC‑CAGGGGGTGGGTAAAAGGGCGGGTGTTGGGGGGGAAGAATTAC‑GGGGGGCGCTGGCACAggggt                                               >  1:467275/1‑148 (MQ=255)
                                                                                                             ccggCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑A‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTc                                                <  1:2651783/147‑1 (MQ=255)
                                                                                                               tGCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGCGGGAAAAAAACTGGGAGGAAACCGCCCCGG‑AAGGC‑TTTTTTCACAAGGGGGTTGGTTAAA‑GCGCGGGTGTTGGGGGGAAAGAATCCC‑CGGAGCCGCTGGAACAGGGCTgg                                             >  1:373388/1‑150 (MQ=255)
                                                                                                               cccgccGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTg                                              <  1:1648228/146‑1 (MQ=255)
                                                                                                                gCCCCGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGCGGGAAAAAAACTGGCAAACAGCCGCCGC‑G‑CAGGCATTTTTTCAC‑ATTGCGGTTGGTTAATGGCCGGTTGTTGGCGGAAATTAAATAC‑GGGAGCCCCTTACAACGGGCTGCg                                            >  2:2393616/1‑151 (MQ=255)
                                                                                                                gCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGGAAAAAAACTGGCAGACAGCCGCCCC‑G‑AAAGCATTTTTTCAC‑ATGGCGGTGGGTAAATGCGCGGGTGTTGGCGGGAATGAAACAC‑CGTAGCCGCCGACACAGGGCTGCg                                            >  2:1049063/1‑151 (MQ=255)
                                                                                                                 ccccGCCACTACTGGAGAGAACGGCGGTTTTTTTTGGGGGGGAAAAAAGCCTGCCGAAAGACGGCCC‑G‑GAGGAATTTTTTCAC‑AAAGCGTTGGGTAAAAGGGCGGGTTTTTGCGGGAAAGAGATAC‑GGGAGACGCTggaaaaaggcttcgg                                           >  1:1518392/1‑137 (MQ=255)
                                                                                                                 ccccGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGCGGGAAAAAAACTGGCCGAAAGCCGCCCC‑G‑AAGGGAATTTTTTAC‑AAGGCGTTGGATAAAAGCGCGGGTGTTGGCGGGAAAGAATCAC‑GGGAGCCGCCGACACAGGGCTGCgg                                           >  2:303562/1‑151 (MQ=255)
                                                                                                                  cccGCCACTACTGGAGAGAACGGCGGTTTTTTTTCGGCGGGAAAAAAACTGGGAGACAGCCCCCCC‑G‑CAGGCCTTTTTTTAC‑CTGGGGGTTGGGAAAAGCGCGGGTGTTTGGGGGAAAGAAACAC‑CGGAGGCGCCGACACAAGGCTggggg                                          >  1:1028907/1‑150 (MQ=255)
                                                                                                                  cccGCCACTACTGGAGAGAACGGCGGTTTTTTTTCGGCGGGAAAAAAACTCGGCGAAAGGCGCCCC‑G‑CAGGCCTTTTTTTAC‑ATGGGGGTGGGTAAATGCGCGGGGGGTGGGGGGGAAGAATCAC‑GGGGGGCGCTGAcacaaccttgtcgc                                          >  1:1168569/1‑141 (MQ=255)
                                                                                                                  cccGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGGGGAAAAATAACTGGGAGACAGGCGCCCC‑G‑GAGGAATTTTTGTCC‑ATGGCGGTGGATAAAAACGCGGGTGTTGGGGGGAAAGAATCACGGGTAG‑CGCTGACACAAGTCTGTggg                                          >  2:2539666/1‑150 (MQ=255)
                                                                                                                  cccGCCACTACTGGAGAGAACGGCGGATTTTTTGCGGCGGAAAAAAAACTCGCAAACAACCGCCGC‑G‑AAAGCCTTTTTTCAC‑ATAGGGGTGGGTTAAAGCGCGGGTTTTTGCGGGAAAGAAACCC‑GGTTAGCGCTGAAACAGGCCTGccgg                                          >  2:1728220/1‑148 (MQ=255)
                                                                                                                   ccGCCACTACTGGAGAGAACGGCGGTTTTTTTGCGGCGGGAAAAAAGCTTGCAGACAGCCGCCGC‑G‑GAGGCCTTTTTTTCC‑ATGGCGGTTGGTAAATGCGCGGGTGTTGGGGGGAAAGAATAAC‑GGTAGGCGCCGaggtcgggagagcggc                                         >  1:1644484/1‑136 (MQ=255)
                                                                                                                   ccGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTg                                                         <  2:1644489/135‑1 (MQ=255)
                                                                                                                   ccGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCt                                         <  2:610265/151‑1 (MQ=255)
                                                                                                                          tactGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACCGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCg                                            <  1:240575/141‑1 (MQ=255)
                                                                                                                           actGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACATGTCTGCGGCTGGAAtt                                   <  1:1142442/149‑1 (MQ=255)
                                                                                                                                agagaACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTg                                  <  2:321198/145‑1 (MQ=255)
                                                                                                                                   gaACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATgcg                         <  2:373387/151‑1 (MQ=255)
                                                                                                                                     aCGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCt                       <  1:1728215/151‑1 (MQ=255)
                                                                                                                                      cggcggTTTTGTTGCGGCGGAAAAATAGCTGGCAGACCGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGGTGTTGGCGGAAATGAATTAC‑GGTAGCCGCTGACACAGGGCTGCGGCTGGGATTGCCTGATgagatt                      >  2:1043603/1‑146 (MQ=18)
                                                                                                                                      cggcggTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATg                           <  1:1043602/146‑1 (MQ=255)
                                                                                                                                       ggcggTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTAc                     <  2:920802/151‑1 (MQ=255)
                                                                                                                                       ggcggTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGGTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTAc                     >  1:1789692/1‑151 (MQ=255)
                                                                                                                                        gcggTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACg                    <  1:425499/151‑1 (MQ=255)
                                                                                                                                         cggTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGc                   >  1:2210305/1‑151 (MQ=255)
                                                                                                                                             tttGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTAt               >  2:615410/1‑151 (MQ=255)
                                                                                                                                              ttgttgCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTAt               <  2:1789697/150‑1 (MQ=255)
                                                                                                                                               tgttgCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCtt                 >  2:1764045/1‑147 (MQ=255)
                                                                                                                                               tgttgCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCtt                 <  1:1764040/147‑1 (MQ=255)
                                                                                                                                               tgttgCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTATc              >  1:183508/1‑150 (MQ=255)
                                                                                                                                                  tgCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCtt                 >  1:2047256/1‑144 (MQ=255)
                                                                                                                                                  tgCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCtt                 <  2:2047264/144‑1 (MQ=255)
                                                                                                                                                    cggcggAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTATCa             >  2:1347036/1‑146 (MQ=255)
                                                                                                                                                     ggcggAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTATCAGGCCTa       <  1:2388191/151‑1 (MQ=255)
                                                                                                                                                      gcggAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTATCAGGCCTAc      <  1:1927972/151‑1 (MQ=255)
                                                                                                                                                        ggAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGACGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGa    <  1:2574411/151‑1 (MQ=255)
                                                                                                                                                         gAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTATCAGGCCTACg     >  2:113623/1‑149 (MQ=255)
                                                                                                                                                         gAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAg   <  1:2393605/151‑1 (MQ=255)
                                                                                                                                                          aaaaaTAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTATc              >  1:769720/1‑139 (MQ=255)
                                                                                                                                                          aaaaaTAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTATc              <  2:769721/139‑1 (MQ=255)
                                                                                                                                                          |                                                                                                                                                          
GAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTT‑CCACATTGTT‑ACCAGCAGCGATTGCCAC‑ACAACATGACGGGCTT‑AAA‑GCTATTT‑CTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTTGTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGC‑G‑AAGGCATTTTTGCAC‑ATGGCGTTGGATAAATGCGCGGTTGTTGGCGGAAATGAATCAC‑GGTAGCCGCTGACACAGGTCTGCGGCTGGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGT  >  DH1/3512927‑3513225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑