Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,135,7790TG36.4% 340.3 / 24.5 160V48G (GTA→GGA) ampCBlaEC family class C beta‑lactamase
Reads supporting (aligned to +/- strand):  ref base T (14/84);  new base G (56/0);  total (76/84)
Fisher's exact test for biased strand distribution p-value = 4.19e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

CAGACCCTATGTTCAAAACGACGCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTGGCGTGCTTGGTGGCGACGCTATTGCTCGAGGGG  >  DH1/4135629‑4135928
                                                                                                                                                         |                                                                                                                                                        
cAGACCCTATGTTCAAAACGACGCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATggcgg‑t‑ggc‑ggt                                                                                                                                                          <  1:535734/151‑1 (MQ=255)
   ccccTATGTTCAAAACGACGCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AAt                                                                                                                                                      <  1:605924/150‑1 (MQ=255)
        aTGTTCAAAACGACGCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTa                                                                                                                                                   <  2:861385/149‑1 (MQ=255)
         tGTTCAAAACGACGCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGG‑AAtttttt                                                                                                                                                 >  1:1081970/1‑147 (MQ=255)
           ttCAAAACGACGCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGG‑AATTTTTTA‑gg                                                                                                                                             >  1:1205426/1‑151 (MQ=255)
             cAAAACGACGCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGt                                                                                                                                           <  1:2329163/151‑1 (MQ=255)
             cAAAACGACGCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGt                                                                                                                                           <  1:1930089/151‑1 (MQ=255)
             cAAAACGACGCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGG‑AATTTATCA‑aggg                                                                                                                                           >  2:2555719/1‑150 (MQ=255)
              aaaaCGACGCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGG‑GGG‑AATTTATTA‑gggg                                                                                                                                           >  2:2575435/1‑149 (MQ=255)
              aaaaCGACGCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTa                                                                                                                                          <  1:917637/151‑1 (MQ=255)
                  cgacgCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑TGGGG‑GGG‑ATTTTTTCA‑GGGAAAAc                                                                                                                                       >  1:2178029/1‑151 (MQ=255)
                     cgCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑gg                                                                                                                                             <  2:1324688/141‑1 (MQ=255)
                     cgCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTaaa                                                                                                                                        <  1:328940/146‑1 (MQ=255)
                     cgCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGA‑GGG‑AATTTATTA‑GGGTaaaaaaat                                                                                                                                   >  2:328939/1‑146 (MQ=255)
                      gCTCGGCGCTTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCttat                                                                                                                                  <  2:2061523/151‑1 (MQ=255)
                       ctctGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑a                                                                                                                                                        <  1:2255720/129‑1 (MQ=255)
                        tctGCGCCTTATAAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCttat                                                                                                                                  <  2:768020/149‑1 (MQ=255)
                         ctGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑TGC‑GGG‑AATTTTTTA‑CGGAAAAACttatttt                                                                                                                               >  1:2487109/1‑149 (MQ=255)
                         ctGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCttatt                                                                                                                                 <  1:2575420/149‑1 (MQ=255)
                         cgacaccTTATTAATTACCGTCTCTTGCTCCACATTTGCTGCCCATCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAACCCttatta                                                                                                                                <  1:754377/145‑1 (MQ=255)
                          tGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGG‑AAATTTTTA‑GGGGAAAAATTATTTCt                                                                                                                              >  1:1778322/1‑151 (MQ=255)
                           gcgcCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCttatt                                                                                                                                 <  2:1960461/147‑1 (MQ=255)
                           gcgcCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTAc                                                                                                                               <  2:2423601/149‑1 (MQ=255)
                           gcgcCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACtt                                                                                                                             <  2:2178037/151‑1 (MQ=255)
                            cgcCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACttt                                                                                                                            <  2:2487121/151‑1 (MQ=255)
                             gcCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTa                                                                                                                           <  2:2127340/151‑1 (MQ=255)
                                 gattaattaCCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTAcc                                                                                                                         <  1:1584744/148‑1 (MQ=255)
                                  attaattaCCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTTTTA‑GGGGAAAACttttttattttactat                                                                                                                      >  1:968572/1‑140 (MQ=255)
                                    taattaCCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AAtt                                                                                                                                                     <  2:325046/119‑1 (MQ=255)
                                      attaCCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCt                                                                                                                  <  1:1271910/151‑1 (MQ=255)
                                         aCCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCt                                                                                                                                     <  2:2000604/129‑1 (MQ=255)
                                           cGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGG‑AATTTATTA‑GGGGAAACCTTTTTTCtttttcttgggggatcgg                                                                                                             >  2:2493587/1‑136 (MQ=255)
                                              ctctTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGG‑AATTTTTTA‑GGGTTAACCTTTTTAATTTTCCTGGGGGTAttagg                                                                                                            >  1:2050764/1‑145 (MQ=255)
                                               tctTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGG‑AAATTTTCA‑GGGGAAACCTTATTACTTTTCCTGGGGCTATGCGGAc                                                                                                          >  2:295652/1‑150 (MQ=255)
                                                    cTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGG‑GAATTATCA‑AGGTAAACACTTTTACTTTAACTCGGGGTTTGTGGGCATCGcc                                                                                                    >  1:1611484/1‑151 (MQ=255)
                                                     tCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGG‑GGG‑AATTTATTA‑GGGGAAAACTTTTTTATTTACCTGGGGCTATTGGGACATCGccc                                                                                                   >  2:459481/1‑150 (MQ=255)
                                                         caTTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCaaaaa                                                                                               <  2:1199806/151‑1 (MQ=255)
                                                               ctgcCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAagca                                                                                            <  1:879134/148‑1 (MQ=255)
                                                                tgcCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AAATTATCA‑CGGGAAACCTTATTACTTTAACTAGGGGCAAGCGGGCACTGCCCAAAAACAGccc                                                                                        >  2:642488/1‑151 (MQ=255)
                                                                 gcCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGccc                                                                                        <  1:2052716/150‑1 (MQ=255)
                                                                 gcCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGccc                                                                                        <  2:2050772/150‑1 (MQ=255)
                                                                 gcCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGccc                                                                                        <  1:295653/150‑1 (MQ=255)
                                                                 gcCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGccc                                                                                        <  2:1633823/150‑1 (MQ=255)
                                                                  ccccTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGG‑GGT‑GATTTATTA‑TGGTAAAACTTATTACTTTATCTGGGGGGATTCGGACATCGCCCAAAAAGAGCCCGt                                                                                      >  1:559244/1‑151 (MQ=255)
                                                                        aacaaATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑TGG‑CGA‑AATTTTTTA‑CGGTAAAAATTTTTATCTTTCCCGGGGGTAGGCGGGAATTGCCAAAAAGCAGgcccccacca                                                                                 >  1:676515/1‑143 (MQ=255)
                                                                        aacaaATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGTAAATTTTTAA‑GGGAAAACC‑TATTACATTTACTGGGGGGATGCGGGCATTCCCAAAAAAAAACCCCTcacac                                                                                 >  1:80408/1‑150 (MQ=255)
                                                                         acaaATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAg                                                                               <  1:473464/151‑1 (MQ=255)
                                                                          caaATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGc                                                                              <  2:889089/151‑1 (MQ=255)
                                                                            aaTCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑aa                                                                           <  1:1352743/151‑1 (MQ=255)
                                                                             aTCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGG‑GATTTTTTA‑GGGGAAAACTTTTTACTTTTTCTCGGGGTATTCGGGCAACGCCCAAAAACAGCCCCTCACCcaac‑aa                                                                           >  1:1735818/1‑150 (MQ=255)
                                                                             aTCAACGATATTGTGCATCGCACAATTACCCCGCTTAAAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGG‑AATTTATTA‑GGGGAAAACCTTTTACTTTTACTGGGGGTATTGGGGCATCGCCCAAAAAAAAACCCTCACAcaac‑aa                                                                           >  1:1154366/1‑150 (MQ=255)
                                                                               cAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGGCG‑GGG‑GGG‑ATTTTTTCA‑GGGGAAACTTTTTTA‑TTTACCTTGGGGCATGTGGGCATCGCCAAAAAAAAGCCCCTCACACCAC‑aaaac                                                                        >  2:122626/1‑149 (MQ=255)
                                                                                 aCGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACgtt                                                                      <  2:80408/151‑1 (MQ=255)
                                                                                 aCGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACgtt                                                                      <  2:1768869/151‑1 (MQ=255)
                                                                                  cGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACgttg                                                                     <  1:755344/151‑1 (MQ=255)
                                                                                   gATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGG‑GGC‑AATTTTTTA‑AGGTAAAACTTATTATCTTTCCCGGGGCTATGCGGACAACGCCAAAAAAAAGCCCGTCCCAAAGC‑AAACgttgt                                                                    >  1:99714/1‑151 (MQ=255)
                                                                                     tatTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGG‑GGG‑AATTTTTTA‑GGGGAAAACTTTTTTCTTCACCTGGGGCTATGCGGACATCGCCCAAAAACAACCCCTCACACAGC‑AAAAGTTGttt                                                                  >  2:1386584/1‑151 (MQ=255)
                                                                                      atTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGttt                                                                  <  1:215554/150‑1 (MQ=255)
                                                                                        tgtgCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGa                                                                <  2:559245/150‑1 (MQ=255)
                                                                                         gtgCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGt                                                              <  1:2424814/151‑1 (MQ=255)
                                                                                         gtgCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGt                                                              <  1:882695/151‑1 (MQ=255)
                                                                                           gCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑TTG‑GGG‑GATTTATCA‑GGGGAAAACATTTTTATTTTCCTCGGGGTATGTGGACATCACCCAAAAACAGCCCGTCTCCCAGCAAAACGtttttttggtt                                                             >  1:1039622/1‑146 (MQ=255)
                                                                                           gCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTa                                                            <  1:2365482/151‑1 (MQ=255)
                                                                                           gCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTa                                                            <  2:801075/151‑1 (MQ=255)
                                                                                            cATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAg                                                           <  1:2555017/151‑1 (MQ=255)
                                                                                             aTCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAgg                                                          <  1:1594238/151‑1 (MQ=255)
                                                                                             aTCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGG‑AATTTTTCA‑AGGGAAAACTTATTTCTTTTCTTTGGGCTATGCGGGCATCCCCAAAAAACAACCCCTCACACAGC‑CAACGTTGTTTTAGTTTgg                                                          >  2:2532092/1‑151 (MQ=255)
                                                                                               cGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGtt                                                        <  2:1081971/151‑1 (MQ=255)
                                                                                                  acaATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGG‑GGT‑AAATTTTCAGGGGAAAACCTTTTTATTTTACTTGGGG‑TATGCGGACATTGCCAAAAAACAGCCCCGCACACAGC‑AAAAATTTTTTTAGTTaaggtta                                                      >  2:1722059/1‑144 (MQ=255)
                                                                                                  acaATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTc                                                       <  1:1722054/149‑1 (MQ=255)
                                                                                                    aaTTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACTTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTc                                                   <  1:2532079/151‑1 (MQ=255)
                                                                                                     aTTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGG‑AATTTTTTA‑GGGGAAAACTTATTATTTTTCCTGGGGGTATGCGGGCATCGCCAAAAAAAAGCCCCTCCCACAGC‑AAAAGGTGTTTTTGGTAGGGTCgggc                                                   >  2:1814682/1‑148 (MQ=255)
                                                                                                       tACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAg                                                 <  2:2475156/150‑1 (MQ=255)
                                                                                                          cccGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCaaa                                             <  2:1586379/151‑1 (MQ=255)
                                                                                                           ccGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGG‑AAATTATTA‑AGGGAAAACTTATTTCTTTTCCTGGGGGTATGCGGGCATCGCCAAAAAACAGCCCGTCACACAGC‑AAAAGTTGTTTTAGTTTGGGTTGGGCAGCaaa                                             >  2:2108114/1‑150 (MQ=255)
                                                                                                             gCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGG‑GGG‑GAATTATTA‑GGGGAAAAATTTTTAATTTTTCTTGGGGTATTCGGGCATTGCCAAAAAAAAACCCCACACACACC‑AAAAGATGTTTGTGGTTGGTTCGGGGCGCaaaaa                                           >  1:2418178/1‑149 (MQ=255)
                                                                                                             gCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTAATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCgg                                                     <  1:2659166/140‑1 (MQ=255)
                                                                                                               ttATAGAGCAACAAAAGATCCCGGGTATGGCGG‑C‑GGG‑GGG‑GATTTTTCT‑GGGTAAAACTTATTACTTTTCCCCGGGGGCTGCGGATAAAGACAAAAAACAGCCCGTCACACACC‑AAACGGTGTTTTAGTTAGGTTCGGGCAGGAAAACAtt                                        >  1:1280851/1‑151 (MQ=255)
                                                                                                                tataGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGCGGGA‑AATTTTTAA‑GGGAAAACCTT‑TTTCTTTTCCTGGGGGTATTCGGACATCGCCCAAAAAAAACCCGCCCCACAAC‑AAAAGTTGTTTTTGTTTGGGTTGGTCAGCaaaaaaatt                                       >  2:2651361/1‑146 (MQ=255)
                                                                                                                tataGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGG‑GATTTTTTA‑GGGTAAAACTTATTAATTTTCCTGGGGGTATGCGGGCATCGCCCAAAAAAAACCCGTCACACAAC‑AAAAGGTGTTTGAGTTAGGTTTGGTCAGCaaaaaaatt                                       >  1:2104238/1‑146 (MQ=255)
                                                                                                                 ataGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGG‑GGG‑AAATTATCA‑GGGTAAAAATTATTTCTTTTTCTGGGGGGATGCGGACATCTCCAAAAAACAGCCCCTCACACAGC‑AAACGTTGTTTGAGGTTGGGTTGGTCAGCaaaaaaattt                                      >  1:797/1‑145 (MQ=255)
                                                                                                                   tgagCAACAAAAGATCCCGGGTATGGCGGCA‑GGG‑GGG‑ATTTTACAA‑GAGGAAA‑CTTATTACTTTATCTGTGGGTGTGCCGATAACGCCCAAAAATAACCCGCCACAAAAC‑AAACCGTGTTTTATTTAAGTTTTGTCAGGCAAAAATTTACt                                    >  1:1985929/2‑151 (MQ=255)
                                                                                                                   agagCAACAAAAGATCCCGGGTATGGCGG‑T‑GGG‑GGG‑AATTTTTTA‑GGGGAAAACCTATTACTTTTCCTGGGGGTATGGGGGCATCGCCAAAAAACAACCCGTCACACAGC‑AAAAGTTGTTTGTGTTTGGTTTGGGCAGCAAAAAATTTTCt                                    >  2:1129540/1‑151 (MQ=255)
                                                                                                                      gCAACAAAAGATCCCGGGTATGGCGG‑T‑GGG‑GGG‑AAATTATTC‑GGGTAAAACTTATTTCTTTATCCGGGGGTATGCGGACATCGCCCAAAAACAGCCCCCCACACACC‑AAACGTTGTTTTTGTTAGGTTCGGGCAGCAAAACAatttatg                                   >  1:1816931/1‑145 (MQ=255)
                                                                                                                      gCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTg                                   <  2:1816936/149‑1 (MQ=255)
                                                                                                                       caacaaAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTGGCg                                <  2:1567111/151‑1 (MQ=255)
                                                                                                                        aacaaAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTGGCGt                               <  2:1039623/151‑1 (MQ=255)
                                                                                                                           aaaaGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTGGCGTGCt                            <  2:99714/151‑1 (MQ=255)
                                                                                                                            aaaGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTGGCGTGCtt                           <  2:1858463/151‑1 (MQ=255)
                                                                                                                                aTCCCGGGTATGGCGG‑T‑GGG‑GGG‑AATTTTTTA‑GGGTAAAACTTTTTTCTTTTTCTGGGGGTATGCGGGCATCGCCCAAAAAAAACCCGTCACACAAC‑AAAAGTTGTTTGAGTTAGGTTTGGGCAGCAAAACATTTTATGGCGTGCTtggtg                       >  1:2113153/1‑151 (MQ=255)
                                                                                                                                       gTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAg                                                 <  2:1741355/118‑1 (MQ=255)
                                                                                                                                       gTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTGGCGTGCTTGGTGGCGACGc                <  2:1726594/151‑1 (MQ=255)
                                                                                                                                           ggcgg‑t‑ggc‑ggt‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTGGCGTGCTTGGTGGCGACGCTAtt            <  2:449053/151‑1 (MQ=255)
                                                                                                                                           ggcgg‑t‑ggc‑ggt‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTGGCGTGCTTGGTGGCGACGCTAt             <  2:1280853/150‑1 (MQ=255)
                                                                                                                                            gcgg‑t‑ggc‑ggt‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTGGCGTGCTTGGTGGCGACGCTATTg           <  1:452834/151‑1 (MQ=255)
                                                                                                                                              gg‑t‑ggc‑ggt‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTGGCGTGCTTGGTGGCGACGCTATTGCt         >  1:2118087/1‑151 (MQ=255)
                                                                                                                                               g‑t‑ggc‑ggt‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTGGCGTGCTTGGTGGCGACGCTATTGCTc        <  2:256861/151‑1 (MQ=255)
                                                                                                                                                   ggc‑ggt‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTGGCGTGCTTGGTGGCGACGCTATTGCTCGa      <  2:2300140/151‑1 (MQ=255)
                                                                                                                                                       ggt‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTGGCGTGCTTGGTGGCGACGCTATTGCTCGAggg   <  2:509979/151‑1 (MQ=255)
                                                                                                                                                         t‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTGGCGTGCTTGGTGGCGACGCTATTGCTCGAgggg  >  2:1569851/1‑150 (MQ=255)
                                                                                                                                                         |                                                                                                                                                        
CAGACCCTATGTTCAAAACGACGCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGCACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGG‑T‑GGC‑GGT‑AATTTATCA‑GGGTAAACCTTATTACTTTACCTGGGGCTATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGC‑AAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTGGCGTGCTTGGTGGCGACGCTATTGCTCGAGGGG  >  DH1/4135629‑4135928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑