Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,181,7900TG37.1% 402.6 / 29.4 178V161G (GTG→GGG) basStwo‑component sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base T (11/99);  new base G (65/0);  total (79/99)
Fisher's exact test for biased strand distribution p-value = 6.67e-37
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

GCCCATTGCCATTCACAGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCATTTTCTTCCGGTAATTATCAACATGT  >  DH1/4181641‑4181940
                                                                                                                                                     |                                                                                                                                                      
gCCCATTGCCATTCACAGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTg                                                                                                                                                       <  1:1384186/151‑1 (MQ=255)
  ccATTGCCATTCACAGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGt                                                                                                                                                        <  1:700385/148‑1 (MQ=255)
  ccATTGCCATTCACAGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCg                                                                                                                                                     >  2:1926287/1‑151 (MQ=255)
   cATTGCCATTCACAGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGt                                                                                                                                                    <  2:1010805/151‑1 (MQ=255)
      tGCCATTCACAGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGttt                                                                                                                                                  <  2:1609369/150‑1 (MQ=255)
       gCCATTCACAGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTg                                                                                                                                                 <  1:2395444/150‑1 (MQ=255)
         cATTCACAGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCAt                                                                                                                                              <  1:109840/151‑1 (MQ=255)
         cATTCACAGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGGGGTTGCAt                                                                                                                                              >  1:1736433/1‑151 (MQ=255)
          aTTCACAGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGGGGTTGCaac                                                                                                                                             >  1:1042328/1‑149 (MQ=255)
          aTTCACAGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGTTTGGATc                                                                                                                                             >  2:948596/1‑151 (MQ=255)
          aTTCACAGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGTTTGCAtt                                                                                                                                             >  1:1065488/1‑150 (MQ=255)
           ttCACAGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGATGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGTTTGCctct                                                                                                                                            >  2:2557921/1‑151 (MQ=255)
           ttCACAGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGGTTTCATCt                                                                                                                                            >  2:1106208/1‑151 (MQ=255)
             cacaGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTgg                                                                                                                                          <  2:1042329/151‑1 (MQ=255)
              acaGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCAt                                                                                                                                              <  2:413782/146‑1 (MQ=255)
                  cgcCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAActg                                                                                                                                     <  1:655486/151‑1 (MQ=255)
                     cACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAActgctg                                                                                                                                  <  1:2366786/151‑1 (MQ=255)
                      aCCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTgg                                                                                                                                 <  1:519969/151‑1 (MQ=255)
                       cccTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGc                                                                                                                                <  1:2020157/151‑1 (MQ=255)
                        ccTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCg                                                                                                                               <  2:924254/151‑1 (MQ=255)
                        ccTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGCTTTCCACTGGCAATGGTGGCg                                                                                                                               >  2:635308/1‑151 (MQ=255)
                            gAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGaaaa                                                                                                                           <  2:2443259/151‑1 (MQ=255)
                             aaaTCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTTCCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAAc                                                                                                                          <  2:221068/151‑1 (MQ=255)
                                 cGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCa                                                                                                                       <  2:1065489/150‑1 (MQ=255)
                                   aaGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGGTTTCATCTGGAAATGCTGGCGCAAACGCATc                                                                                                                     >  2:1748160/1‑150 (MQ=255)
                                     gCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGGGGTTGCATCTGGGAATGCTGGCGGAAACGCCTcac                                                                                                                   >  1:2050288/1‑150 (MQ=255)
                                        gtggtTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTg                                                                                                               <  2:579203/151‑1 (MQ=255)
                                        gtggtTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTg                                                                                                               <  1:948595/151‑1 (MQ=255)
                                         tggtTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGGTTTCATCTGGAAATGCTGGCGAAAACGCAACACAttt                                                                                                               >  1:517432/1‑149 (MQ=255)
                                          ggtTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGGTTTCATCTGGAACTGCTGGCGAAAAAGCATCACATTTAt                                                                                                             >  2:14569/1‑151 (MQ=255)
                                            tttCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGTTTGCATCTGGAACTGCTGGCGAAAAAGCATCACATTGATGt                                                                                                           >  1:741365/1‑151 (MQ=255)
                                             ttCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGTTTGCATCTGGAACTGCTGGCGAAAAAGCATCACATTTATGt                                                                                                           >  2:742159/1‑150 (MQ=255)
                                             ttCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGTTTGCATCCTGAACTGCTGGCGAAAAAACATCACACTTATGt                                                                                                           >  1:1513245/1‑150 (MQ=255)
                                              tCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGGTTTCATCTGGAAATGCTGGGGGAAAAGCATCACATTTATGt                                                                                                           >  1:809665/1‑149 (MQ=255)
                                               cGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAg                                                                                                         <  1:472521/150‑1 (MQ=255)
                                               cGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGc                                                                                                        <  2:2530788/151‑1 (MQ=255)
                                                ggCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGGGGTTGCATCTGGGAATGCTGGGGAAAAAGCAACACATTGATGTaac                                                                                                        >  2:1933569/1‑148 (MQ=255)
                                                 gCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCAc                                                                                                      <  2:809666/151‑1 (MQ=255)
                                                  cGTTAAACGATCTGGTGAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGATGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCAcc                                                                                                     <  1:2557906/151‑1 (MQ=255)
                                                   gTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCg                                                                                                    <  2:440695/151‑1 (MQ=255)
                                                     tAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGt                                                                                                   <  1:142101/150‑1 (MQ=255)
                                                      aaaCGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGtt                                                                                                  <  2:741366/150‑1 (MQ=255)
                                                      aaaCGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTa                                                                                                 <  2:1736438/151‑1 (MQ=255)
                                                        aCGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGGGTTTGCCCATGGAAACGGTGGCGCAAAAGGAACCCCCTGATGTAGCACCGTTAGt                                                                                               >  2:162209/1‑151 (MQ=255)
                                                          gATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAAc                                                                                                                          <  2:56289/122‑1 (MQ=255)
                                                            tCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCAt                                                                                                                      <  1:673887/124‑1 (MQ=255)
                                                               ggTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTg                                                                                              <  2:517432/145‑1 (MQ=255)
                                                               ggTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTg                                                                                              <  1:1492328/145‑1 (MQ=255)
                                                               ggTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGGGTTTTCATCTGGAACTGCTGGCGAAAAAGCATCACAATTATGTAGCCCCCTTAGTGGCAAgg                                                                                        >  2:1198789/1‑151 (MQ=255)
                                                                  cAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGGGGTTGCATCTGGAACTGCTGGCGAAAAAGCATCCCAATGATGTAGCACCGTTAGTGGGACGGCtt                                                                                     >  1:1528542/1‑151 (MQ=255)
                                                                  cAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGTTTTCATCTGGAACTGCTGGGGGAAAAGCATCACATTTATGTAGCACCGTTAGTGGCACGGCtt                                                                                     >  2:2538942/1‑151 (MQ=255)
                                                                   aGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGGTTGCATCTGGGACTGCCTGCGGAAAACCATCAAATTGGTGTAGCACCGTTTGTGGGACCGCTTg                                                                                    >  2:1295361/1‑151 (MQ=255)
                                                                    gTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGTTTGCCTCTGGAACTGCTGGCGGAAACGCATCACATTTATGTAGCACCGTTTGTGGGACGGCTTgt                                                                                   >  2:1375180/1‑150 (MQ=255)
                                                                      cgtcTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTgg                                                                                             <  2:685037/136‑1 (MQ=255)
                                                                       gCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCa                                                                                <  1:2220695/151‑1 (MQ=255)
                                                                          tGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGGTTTCCTCTGGAAATGCTGGCGAAAACGCATCACATTTATGTAGCACCGTTAGTGGCACGGCTTGATc                                                                                 >  2:2453175/1‑147 (MQ=255)
                                                                              cAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGatgatg                                                                         <  1:14569/151‑1 (MQ=255)
                                                                               aGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATgg                                                                        <  1:1295359/151‑1 (MQ=255)
                                                                               aGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATgg                                                                        <  1:1198787/151‑1 (MQ=255)
                                                                               aGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATgg                                                                        <  2:1513250/151‑1 (MQ=255)
                                                                                  aCGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGgaga                                                                     <  2:20114/151‑1 (MQ=255)
                                                                                    gCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTa                                                                                                 <  2:423585/121‑1 (MQ=255)
                                                                                    gCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGc                                                                   <  1:1395432/151‑1 (MQ=255)
                                                                                       ggATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGTTTGCATCTGGGACTGCCGGCGGAAACGCATCACCATGATGGTGCACCGTTTGTGGGCCGGCTTGATCAGATGGTGGagaggggc                                                                >  1:135304/1‑147 (MQ=255)
                                                                                        gATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGtct                                                               <  2:1394264/151‑1 (MQ=255)
                                                                                        gATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGtct                                                               <  1:1933563/151‑1 (MQ=255)
                                                                                          tAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGCGGTGCGTTTTTCTTTTGAACTGCCGGCGAAAACGCATCAAACTTATTTAGCACCGTTTGTGGGACGGCTTGGTTAGATGATGGTGAGCCTCTcc                                                             >  2:204460/1‑151 (MQ=255)
                                                                                            aCGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCa                                                           <  2:1528547/151‑1 (MQ=255)
                                                                                            aCGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCa                                                           <  1:1926282/151‑1 (MQ=255)
                                                                                                aaGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAgctg                                                       <  1:1271479/151‑1 (MQ=255)
                                                                                                      gttTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAGCTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAg                                                          <  1:2576464/142‑1 (MQ=255)
                                                                                                      gttTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAgct                                                        <  1:1615419/144‑1 (MQ=255)
                                                                                                          aCCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTgg                                             <  2:595225/151‑1 (MQ=255)
                                                                                                          aCCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGGTTTCATCTTGGACTGCTGGCGGAAACGCATCACCTTGATGGAGCACCGGTTGTGGCACGGCTTGATTAGATGATGGAGAGCGGCTCCCCACTGCTGCAACTgg                                             >  1:412679/1‑151 (MQ=255)
                                                                                                          aCCACTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTg                                              <  1:775628/150‑1 (MQ=255)
                                                                                                           ccGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGGTTGCATCTGGAAATGCTGGCGAAAAAGCATCACATTTATGTAGCACCGTTAGTGGCACGGCTTTATCAGATGATGGAGAGCGGCTCCCAGCTGCTGCAACTggg                                            >  2:209448/1‑150 (MQ=255)
                                                                                                             gCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGgcgc                                          <  1:209449/151‑1 (MQ=255)
                                                                                                             gCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGCGTTTTCATCTGGAACTGCTGGCGGAAACGCATCACATTGATGTAGCACCGTTAGTGGGACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGgcgc                                          >  1:1029278/1‑151 (MQ=255)
                                                                                                                gACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGGGGGTTTTCATCTTGAACTGCTGGCGAAAAAGCATCACATTTATGTAGCACCGTTAGTGGGACGGCTTTATCAGATGATGGAGAGGGTCTCCCAGCTGCTTCAAATGGCGCGTg                                       >  2:756604/1‑151 (MQ=255)
                                                                                                                        gcACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAg                               <  1:545010/151‑1 (MQ=255)
                                                                                                                        gcACGAACTGCGAACGCCACTGGCGGGGGGGCGTTTGCATCTTGAACTGCTGGCGAAAAAGCATCACATTGATGTAGCACCGTTAGTGGGACGGCTTGATTAGATGATGGGGAGGGGCTCCCCGCTGCTGCAAATGGGGCGGGCCgggc                                 >  2:1246908/1‑147 (MQ=255)
                                                                                                                              aCTGCGAACGCCACTGGCGGGGGGGGGTTTTCCTCTGGAACTGGTGGCGAAAACGCATCACATTTATGTAGCACCGGTAGTGGCCCGGCCTTATCAGAAGATTGAGAGCGTCTCCCCGCTGCTGCCACTGGCGCGGGGCGGGCAGGCAttt                         >  2:2148623/1‑151 (MQ=255)
                                                                                                                                 gCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCAtttt                        <  2:1316338/149‑1 (MQ=255)
                                                                                                                                 gCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCATTttc                       <  2:1166582/150‑1 (MQ=255)
                                                                                                                                  cGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCATTttctt                     <  2:521093/151‑1 (MQ=255)
                                                                                                                                   gAACGCCACTGGCGGGGGTGGGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACCTTGATGTTGCACCGGTTGTGGCACGGCTTTATCAGAAGATGGGGAGCGTCTCCCCGCTGCTGCAACTGGGGCGGGCCGGGCAGTCATTttcttt                    >  1:270010/1‑150 (MQ=255)
                                                                                                                                   gAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCATTttcttc                    <  1:2453163/151‑1 (MQ=255)
                                                                                                                                       gCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAgctgct                                                     <  2:2219005/114‑1 (MQ=255)
                                                                                                                                        ccACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCATTTTCTTCCGGTa               <  1:1137322/151‑1 (MQ=255)
                                                                                                                                          aCTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCAt                           <  2:2612257/137‑1 (MQ=255)
                                                                                                                                             ggCGGTGGTCCGTTTGCATCTGGAACTTCTGGCGAAAACGCAACACATTGATGTAGCACCGGTAGAGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACCGGGGCGTGGCGGACAGTCATTTTCTTTCGGGAAtagg          >  1:2191915/1‑148 (MQ=255)
                                                                                                                                             ggCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCATTTTCTTCCGGTAAtt            <  2:1549295/149‑1 (MQ=255)
                                                                                                                                             ggCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCATTTTCTTCCGGTAATTAt          <  1:742158/151‑1 (MQ=255)
                                                                                                                                             ggCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCATTTTCTTCCGGTAATTAt          <  1:1246906/151‑1 (MQ=255)
                                                                                                                                               cGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCATTTTCTTCCGGTAATTATCa        <  2:270009/151‑1 (MQ=255)
                                                                                                                                                ggggggGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCATTTTCTTCCGGTAATTATCa        >  2:1643448/1‑150 (MQ=255)
                                                                                                                                                 ggggTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCATTTTCTTCCGGTAATTATCAAc      >  2:2252929/1‑151 (MQ=255)
                                                                                                                                                 ggggTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCATTTTCTTCCGGTAATTATCAAc      <  1:1643443/151‑1 (MQ=255)
                                                                                                                                                   ggTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCATTTTCTTCCGGTAATTATCAACAt    <  1:1388138/151‑1 (MQ=255)
                                                                                                                                                     tGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCATTTTCTTCCGGTAATTATCAACATGt  >  2:66989/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GCCCATTGCCATTCACAGCGCCACCCTCGAAATCGAAGCGGTGGTTTCGGCGTTAAACGATCTGGTCAGTCGCCTGACCAGCACGCTGGATAACGAAAGGTTGTTTACCGCTGACGTCGCGCACGAACTGCGAACGCCACTGGCGGGGGTGCGTTTGCATCTGGAACTGCTGGCGAAAACGCATCACATTGATGTAGCACCGTTAGTGGCACGGCTTGATCAGATGATGGAGAGCGTCTCCCAGCTGCTGCAACTGGCGCGTGCCGGACAGTCATTTTCTTCCGGTAATTATCAACATGT  >  DH1/4181641‑4181940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑