Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1440,4870TG37.4% 295.3 / 47.3 147V274G (GTA→GGA) secYprotein translocase subunit SecY
Reads supporting (aligned to +/- strand):  ref base T (13/79);  new base G (54/1);  total (67/80)
Fisher's exact test for biased strand distribution p-value = 1.86e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

CAGTATTAGTATTTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGCTTTATGTGTTACTCTATGCGTCTG  >  DH1/440337‑440636
                                                                                                                                                      |                                                                                                                                                       
cAGTATTAGTATTTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGt                                                                                                                                                         <  1:1446964/151‑1 (MQ=255)
   tattagtattTGCAGTCAGGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAAt                                                                                                                                                      >  1:39250/1‑151 (MQ=255)
    attagtattTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATc                                                                                                                                                     <  1:5549/151‑1 (MQ=255)
     ttagtattTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATcc                                                                                                                                                    >  1:2246917/1‑151 (MQ=255)
      tagtattTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTGATtcc                                                                                                                                                   >  1:2281964/1‑151 (MQ=255)
       agtattTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATccc                                                                                                                                                   <  2:868087/150‑1 (MQ=255)
       agtattTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATcccc                                                                                                                                                  >  2:1539042/1‑150 (MQ=255)
        gtattTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATacccc                                                                                                                                                 >  1:125048/1‑149 (MQ=255)
         tattTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCggg                                                                                                                                                >  2:1244874/1‑150 (MQ=255)
          attTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCGG‑c                                                                                                                                               >  2:766937/1‑150 (MQ=255)
          attTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCGG‑c                                                                                                                                               >  1:2349980/1‑150 (MQ=255)
             tGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATc                                                                                                                                           >  1:2376768/1‑151 (MQ=255)
              gCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCt                                                                                                                                          <  2:180821/151‑1 (MQ=255)
               cAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCtt                                                                                                                                         <  1:766936/151‑1 (MQ=255)
                aGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTc                                                                                                                                        <  1:1004536/151‑1 (MQ=255)
                     cGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCATCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTc                                                                                                                                   <  1:2644364/151‑1 (MQ=255)
                        tCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCa                                                                                                                                 <  1:2639114/150‑1 (MQ=255)
                        tCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCa                                                                                                                                 <  1:1539037/150‑1 (MQ=255)
                        tCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAAAACCCG‑CAAACTTCGCtttc                                                                                                                                  >  1:1391806/1‑147 (MQ=255)
                        tCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGCGGGAAGAGCGGACAATCTTcctttc                                                                                                                                   >  1:516291/1‑144 (MQ=255)
                             gttgtATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGGGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGtatta                                                                                                                           <  2:21680/151‑1 (MQ=255)
                               tgtATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCCG‑CAATCTTTGCTTCCAGGattatt                                                                                                                         >  2:2224868/1‑151 (MQ=255)
                                gtATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTc                                                                                                                        <  1:1788096/151‑1 (MQ=255)
                                gtATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTc                                                                                                                        <  2:1391811/151‑1 (MQ=255)
                                gtATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAAACTTTGCTTCCAGCattattt                                                                                                                        >  1:2407045/1‑150 (MQ=255)
                                  aTTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCt                                                                                                                       <  2:2492574/150‑1 (MQ=255)
                                     tgttgAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATTCCCG‑CAAATTTCGCCTCCaagaatatttttttt                                                                                                                   >  1:2557156/1‑137 (MQ=255)
                                          aGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGc                                                                                                              <  1:875966/151‑1 (MQ=255)
                                                gTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGCACTCCCG‑CAATATTCCCTTCCAGGAATATTCTTTTACCGCCGACCa                                                                                                         >  2:2006722/1‑150 (MQ=255)
                                                  cAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATcg                                                                                                      <  1:143087/151‑1 (MQ=255)
                                                   aaCGCCGCATTATGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATcgc                                                                                                     <  1:1047895/151‑1 (MQ=255)
                                                    aCGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTccc                                                                                                                 <  1:1623667/138‑1 (MQ=255)
                                                    aCGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAAACCCCG‑CCATCTTCCCTTCCAGTATTATTCTGTTccc                                                                                                                 >  2:1623672/1‑138 (MQ=255)
                                                        cgcATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCttt                                                                                                                     >  2:53265/1‑128 (MQ=255)
                                                          cATTGTGGTAAACTACGCGCAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAAAACCCG‑CCATTTTCTCTGTCACTTGTATTCTGTTTCCCGCGACCATCGCGTcccgg                                                                                              >  1:996083/1‑147 (MQ=255)
                                                             tgtgGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGtt                                                                                            <  1:1762833/150‑1 (MQ=255)
                                                                ggTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCggg                                                                                        <  1:368297/151‑1 (MQ=255)
                                                                ggTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAAATCCCG‑CCATCTTTGCTTTCAGTATTTTTTTTTTTCCGGGGCCCATCGCGTCCTggggatcg                                                                                        >  2:86900/1‑145 (MQ=255)
                                                                   aaaCTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCGG‑CAAACTTCGCTTCCAGTATTATTTTTTTTCCCGCGACCAACCCGTCATGGTTCgggggg                                                                                     >  2:178179/1‑150 (MQ=255)
                                                                   aaaCTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCGG‑CAAACTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCggggg                                                                                      <  1:2006714/150‑1 (MQ=255)
                                                                      cTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCa                                                                                                         <  2:617779/128‑1 (MQ=255)
                                                                      cTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATTCCGG‑CAAAATTTTCTTTCAGGATTATTTTTTTCCCGGCGGCCCTCCCGGCATGGTTTggggggggg                                                                                  >  1:2176873/1‑150 (MQ=255)
                                                                       tACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCGG‑CCATCTTCTCTTCCAGTATTTTTTTTTTTCCCGCGGCCCTCGCGTCCTGGGTTgggggggggg                                                                                 >  1:890003/1‑149 (MQ=255)
                                                                                cGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTTAAGATGGCCGGGGTAATACCGG‑CAATCTTCGATTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGa                                                                        <  1:385911/151‑1 (MQ=255)
                                                                                cGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGa                                                                        <  1:2083270/151‑1 (MQ=255)
                                                                                  tCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCGG‑GAAACTTTGCTTCCCGTATTATTTTGTTCCCGGCGACCATCGACGCATGGTTCGGGGGCGGGACTGGGTggga                                                                       >  2:2254323/1‑148 (MQ=255)
                                                                                   cAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAAc                                                                      <  2:2376778/150‑1 (MQ=255)
                                                                                    aGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCCG‑CCAACTTTGCTTCCCGTATTATTTTTTTTCCGGCGGCCCTCCCGTCATGGGTCGGGGGGGGGACTGGtttggaatg                                                                    >  2:51499/1‑144 (MQ=255)
                                                                                        aGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTg                                                                <  1:51499/151‑1 (MQ=255)
                                                                                         ggTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCGG‑CAATATTCGCTTCCAAAATTATTTTGTTTCCGGCGACCATCCCGTCCTGGGTCGGGGGGGGGACTGGTTgggaaatgctgg                                                               >  2:1596529/1‑141 (MQ=255)
                                                                                             gtcgtGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCGG‑CAATCTTCGCTTCCCGTATTATTCTGTTTCCGGCGACCATCGCGTCCTGGTTCGGGGGCGGGACTGGTTTGGAATGGCTGacaac                                                           >  2:1494287/1‑151 (MQ=255)
                                                                                              tcgtGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGGAAACCGG‑CAATCTTTGCTTCCAGTATTATTTTGTTTCCCGCGACCCTCGCGTCATGGTTCGGGGGGGGGAGTGGTTgggaacggccgaaaac                                                           >  1:943162/1‑136 (MQ=255)
                                                                                              tcgtGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCCG‑CAATCTTCGCTTCCAGTATTTTTCTGTTTCCGGCGACCATCGCGTCATGGGTTGGGGGGGGGACTGGGTGGGAATGGCTGacaac                                                           >  2:2256555/1‑150 (MQ=255)
                                                                                              gtgctctccgatctcacaGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGacaaca                                                          <  2:696142/137‑1 (MQ=255)
                                                                                               cgtGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTa                                                                                 <  2:2595394/127‑1 (MQ=255)
                                                                                               cgtGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGacaaca                                                          <  1:1525974/150‑1 (MQ=255)
                                                                                                gtgtCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGGATTCCGG‑CAATCTTTGCTTCCAGTATTATTTTTTTTCCCGCGACCATCCCGCCATGGGTCGGGGGGGGGGCTGGGTGGGAAAGGCTGGCAACAA‑t                                                       >  2:1690188/1‑151 (MQ=255)
                                                                                                gtgtCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCCG‑CAAACTTTGCTTCCCGTATTATTTTTTTTCCGGCGGCCATCCCGTCATGGTTCGGGGGGGGGACTGGGTGGGACTGGCTGACAACAA‑t                                                       >  2:1169752/1‑151 (MQ=255)
                                                                                                gtgtCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCCG‑CAAAATTTGCTTCCAGTATTATTTTTTTTCCCGCGACCACCGCGTCATGGTTCGGGGGCGGGGCTGGGTGGAACTGGCTGGcaaaaa                                                         >  1:944320/1‑150 (MQ=255)
                                                                                                 tgtCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTg                                                                <  1:2247872/142‑1 (MQ=255)
                                                                                                 tgtCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCGG‑CCATCTTCGCCTTCCGTATTATTTTGTTTCCGGCGACCATCGCGTCATTGTTCGGGGGGGGTACTGGGTGGGAATGGCTg                                                                >  2:2247881/1‑142 (MQ=255)
                                                                                                   tCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATACCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTTCCGGCGACCATCGCGGCATGGTTTGGGGGGGGAATTGGTTGGAAATGGCTGACAACAA‑tttt                                                    >  2:1406385/1‑150 (MQ=255)
                                                                                                      aTGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTc                                                    <  1:1690183/148‑1 (MQ=255)
                                                                                                      aTGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCt                                                 <  1:2256546/151‑1 (MQ=255)
                                                                                                      aTGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAAACCCCG‑CAATCTTCGCTTCCAGTATTTTTCTGTTCCCGGCGACCATCGCGTCATTGTTCGGGGGCGGGAATGGTTGGAACTGGCTGACAACAA‑ATTTGCt                                                 >  2:179641/1‑151 (MQ=255)
                                                                                                      aTGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAAAATCAG‑CAAAATTTGCTTCCCGGATTATTTTGTTTCCGCCGACCATCCCGTCCTGGTTTGGGGGCGGGAATGGTTTGAAAAGGGTGAAAACAA‑ATTccgt                                                 >  1:1233185/1‑148 (MQ=255)
                                                                                                         ctgcACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTc                                                    <  1:247195/145‑1 (MQ=255)
                                                                                                         ctgcACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCCG‑CAATCTTCGCTTCCCGTATTATTTTGTTTCCCGCGACCATCGCGTCATGGTTCGGGGGGGGGACTTGGTGGGACTTGCTGACAAAAA‑atttagaacg                                              >  2:247194/1‑144 (MQ=255)
                                                                                                           gcACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTg                                                                <  2:724056/132‑1 (MQ=255)
                                                                                                           gcACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTAt                                             <  2:981406/150‑1 (MQ=255)
                                                                                                            cacaGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTAttt                                           <  1:2400827/151‑1 (MQ=255)
                                                                                                            cacaGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGGAAACCCG‑CCATCTTCGCCTCCAGGATTATTTTGTTTCCGGCGACCACCCCGTTCTGGTTCGGGGGGGGGACTGGGTGGGAATTGGTGAcaacaaaaacgggagagcg                                            >  1:696141/1‑138 (MQ=255)
                                                                                                             acaGAGCCCACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTg                                          <  2:39250/151‑1 (MQ=255)
                                                                                                             acaGAGCACACATTTACCGCTGAAAGTGAATATGGCGGCGGGAAACCCGG‑CAATTTTTGCTTCCCAAAATTTTTTGTTTCCGGGGCACATCACGGCATTGTTTGGGGGGCGGGCTGGTTTGGAATGGCTGACAAAAA‑ATTTGCTGTAtttt                                          >  2:1543377/1‑150 (MQ=255)
                                                                                                               agagCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTg                                                <  2:1202785/143‑1 (MQ=255)
                                                                                                               agagCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCa                                        <  2:943163/151‑1 (MQ=255)
                                                                                                               agagCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCGG‑CAAACTTTGCTTCCAGTATTATTTTTTTTCCCGCGACCATCGCGTCCTGGGTCGGGGGCGGGACTGGTTGGAACTTGCTTACAACAA‑ATTCGCTgagattggg                                        >  1:1202783/1‑143 (MQ=255)
                                                                                                               agagCACACATTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCGC‑CAAACTTCCCATTCCCAAATATTTTGTTACCCGCGGGCCCCGCCCTTTCGGTCGGGGGAGGTAATGGGTGGAACTGGCTGGCAAAAA‑attttcttgatttttc                                        >  1:398848/1‑138 (MQ=255)
                                                                                                                   cacacaTTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCt                                    <  2:2313213/151‑1 (MQ=255)
                                                                                                                    acacaTTTACCGCTGAAAGTGAATATGGCGGGGGGAATTCCGG‑CAATCTTCGCTTCCAGTATTTTTCTGTTTCCGGCGACCATCGCGTCCTGGTTCGGGGGGGGGACTGGTTGGAACTTGCTGACAACAA‑TTTTTCTGTATTTTTAGCCTg                                   >  2:639593/1‑151 (MQ=255)
                                                                                                                     cacaTTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CCATCTTCGCGTCCAGGATTATTTTTTTTCCGGCGGCCATCGCGTCTTGTGTCCGGGGGGGGGCAGGTTGGCACTGGCTGGCACCAACTTTCGGT‑TATTTGcgcggttg                                  >  2:2513865/1‑144 (MQ=255)
                                                                                                                     cacaTTTACCGCTGAAAGTGAATATGGCGGGGGGAATCCCCG‑CAATCTTCGCTTTCAGTATTATTTTTTTCCCCGCGACCCTCGCGTCATTGTTTGGGGGGGGGACTGGTTGGGACTGGGTGGCAACAA‑TTTTGCTGTATTTTTAGCCCgg                                  >  1:59393/1‑151 (MQ=255)
                                                                                                                            aCCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAAcc                            <  1:639592/150‑1 (MQ=255)
                                                                                                                             ccGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGc                          <  2:925297/151‑1 (MQ=255)
                                                                                                                             ccGCTGAAAGTGAATATGGCGGGGGGAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCCGCGACCATCGCGTCATGGGTCGGGGGCGGGACTGGTTGGGACTGGCTGACAACAA‑ATTTGCTGTATTTTCAGCCTGGGCAAACGc                          >  2:2500824/1‑151 (MQ=255)
                                                                                                                              cGCTGAAAGTGAATATGGCGGGGGGAATCCCCG‑CAATCTTTGCTTCCCGGATTATTCTTTTCCCGGCGACCATCGCGTCATGGTTCGGGGGGGGGACTGGGTGGGACTGGCTGACAACAA‑TTTCGCTGTATTTTTAGCCTGGGCAACCGcc                         >  2:2236381/1‑150 (MQ=255)
                                                                                                                                  gAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGCTTTAt                     <  1:179642/151‑1 (MQ=255)
                                                                                                                                        gAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGCTTTATGTGtt                <  2:1917232/150‑1 (MQ=255)
                                                                                                                                        gAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGCTTTATGTGtt                <  1:2513853/150‑1 (MQ=255)
                                                                                                                                          atatGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGCTTTATGTGTTAct             >  2:129237/1‑151 (MQ=255)
                                                                                                                                          atatGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGCTTTATGTGTTAct             <  1:162781/151‑1 (MQ=255)
                                                                                                                                          atatGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGCTTTATGTGTTAct             <  2:2595390/151‑1 (MQ=255)
                                                                                                                                            atGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGCTTTAtg                    <  2:214102/142‑1 (MQ=255)
                                                                                                                                            atGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGCTTTATGTGTTActc            <  1:627399/150‑1 (MQ=255)
                                                                                                                                            atGGCGGGGGGAATCCCGG‑CAATCTTCGCTTCCAGTATTATTTTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGGACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGCTTTAtg                    >  1:214103/1‑142 (MQ=255)
                                                                                                                                                cGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGCTTTATGTGTTACTCTATGc       >  1:1059068/1‑151 (MQ=255)
                                                                                                                                                 gggggTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGCTTTATGTGTTActct           <  1:952014/146‑1 (MQ=255)
                                                                                                                                                 gggggTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGCTTTATGTGTTActct           >  2:952015/1‑146 (MQ=255)
                                                                                                                                                   gggTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGCTTTATGTGTTACTCTATGCGTc    >  1:800637/1‑151 (MQ=255)
                                                                                                                                                     gTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGCTTTATGTGTTACTCTATGCGTCTg  >  1:71116/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
CAGTATTAGTATTTGCAGTGACGTTCTTTGTTGTATTTGTTGAGCGTGGTCAACGCCGCATTGTGGTAAACTACGCGAAACGTCAGCAAGGTCGTCGTGTCTATGCTGCACAGAGCACACATTTACCGCTGAAAGTGAATATGGCGGGGGTAATCCCGG‑CAATCTTCGCTTCCAGTATTATTCTGTTCCCGGCGACCATCGCGTCATGGTTCGGGGGCGGTACTGGTTGGAACTGGCTGACAACAA‑TTTCGCTGTATTTGCAGCCTGGGCAACCGCTTTATGTGTTACTCTATGCGTCTG  >  DH1/440337‑440636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑