Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913696,5340GT23.7% 283.9 / 38.8 140intergenic (‑30/+8)glnX/glnVtRNA‑Gln/tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base G (34/72);  new base T (2/31);  total (37/103)
Fisher's exact test for biased strand distribution p-value = 2.57e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

GAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACG  >  NC_000913/696385‑696677
                                                                                                                                                     |                                                                                                                                               
gaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTg                                                                                                                                                 <  3:48343/150‑1 (MQ=255)
gaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa                                                                                                                                                <  1:118861/151‑1 (MQ=255)
gaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa                                                                                                                                                <  6:1013198/151‑1 (MQ=255)
 aaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGaa                                                                                                                                               <  4:818577/151‑1 (MQ=255)
  aCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAAt                                                                                                                                              <  3:460981/151‑1 (MQ=255)
  aCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAAt                                                                                                                                              <  4:995568/151‑1 (MQ=255)
  aCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAAt                                                                                                                                              <  4:1274114/151‑1 (MQ=255)
     cAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTGCAAGGTAAGCGTCTTGAATaa                                                                                                                                            >  3:670042/1‑150 (MQ=255)
     cAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATaaa                                                                                                                                           <  8:175206/151‑1 (MQ=255)
      aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGACGGCCGGCATCAGCTGCCGGTGCCTTACAGCTTGGCGCTACGCCAACTGGGTGCTCTTCCAGGGTAAGCGTCTTGacgag                                                                                                                                            >  3:80033/1‑145 (MQ=25)
      aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAt                                                                                                                                          <  5:747794/151‑1 (MQ=255)
       gAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAtt                                                                                                                                         <  4:717651/151‑1 (MQ=255)
       gAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAtt                                                                                                                                         <  7:1553495/151‑1 (MQ=255)
       aaaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAtt                                                                                                                                         <  3:423487/150‑1 (MQ=255)
        aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTg                                                                                                                                        <  8:1276864/151‑1 (MQ=255)
            gCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCAGGTGCCTTACCGCTTGGCGATACCCCAACAAGTTGCAATTTAAAGTTCAGCATATTGACTAAAtatgc                                                                                                                                      >  5:808045/1‑145 (MQ=17)
             cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgg                                                                                                                                   <  7:274221/151‑1 (MQ=255)
             cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgg                                                                                                                                   <  3:1132049/151‑1 (MQ=255)
              aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg                                                                                                                                  <  8:1304287/151‑1 (MQ=255)
              aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg                                                                                                                                  <  1:85718/151‑1 (MQ=255)
               aaaaGCTCGCCGTAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATGCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgggg                                                                                                                                 <  3:1296061/151‑1 (MQ=255)
               aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgggg                                                                                                                                 <  4:1354096/151‑1 (MQ=255)
               aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgggg                                                                                                                                 <  2:690240/151‑1 (MQ=255)
                aaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGt                                                                                                                                <  8:374236/151‑1 (MQ=255)
                 aaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAGTCAGAATCCGGTGGATTACCGCTTGGCGATACCCCAACTGCTTGCAATTAAAATGGCCGCGGCTTGTCGATAGTGGCTGGGGtt                                                                                                                               >  5:395275/1‑150 (MQ=17)
                  aGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCGGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTAc                                                                                                                              <  6:1583162/151‑1 (MQ=255)
                  aGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTAc                                                                                                                              <  6:1590961/151‑1 (MQ=255)
                  aGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTAc                                                                                                                              <  1:1326173/151‑1 (MQ=255)
                   gCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACg                                                                                                                             <  4:569375/151‑1 (MQ=255)
                      ggCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAgg                                                                                                                          <  6:71320/150‑1 (MQ=255)
                      cGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAg                                                                                                                           <  7:386569/150‑1 (MQ=255)
                       gCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGa                                                                                                                         <  7:459681/151‑1 (MQ=255)
                       gCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGa                                                                                                                         <  5:1080202/151‑1 (MQ=255)
                        ccGACGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCTGAATGCCGTAATCAGAATCCGTTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAt                                                                                                                        <  8:1300186/151‑1 (MQ=255)
                        ccGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAGCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCACCATGGTGCTTTTAAAATTTAAGCGGCTTGACGAGAGTGGCTGGGGTAGGTGGAt                                                                                                                        >  4:1679552/1‑151 (MQ=17)
                        ccGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGCATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTGCAAGTGAAGAGGCTTGAATAAAGTGGCTGGGGTTCGTGGAt                                                                                                                        >  4:1145469/1‑151 (MQ=21)
                         cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGTAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCTTCTTGAATAAATTGGCTGGGGTACGAGGAtt                                                                                                                       <  1:975822/151‑1 (MQ=255)
                         cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAtt                                                                                                                       <  4:105545/151‑1 (MQ=255)
                         cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGCCCATACCCCAACTCCGTGCACTTCCAAGGTAAGCGCCTAGAATAAAGAGGCAGGAGTACGAGGAt                                                                                                                        >  7:479368/1‑150 (MQ=21)
                          gtagctagctTTTTTAATGTGGCTGGGGTACGAGGATTCGATACTCGGAATGCCGGAATCAGAATCCTGTGCATTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTc                                                                                                                      <  1:13220/149‑1 (MQ=25)
                           aaGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCg                                                                                                                     <  1:1657980/151‑1 (MQ=255)
                             gCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGaa                                                                                                                   <  3:944083/151‑1 (MQ=255)
                             gCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGaa                                                                                                                   <  6:1435937/151‑1 (MQ=255)
                              cGAGCTTTTTTAATGTCGCTGGGGGACGTGGATTCGATCCTCGGAATGCCGTAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTTCACTTACAACGTAAGGGTCTTTTATAAATTGGCTGGGGTACGAGGATTCGAAc                                                                                                                  <  8:507450/151‑1 (MQ=25)
                               gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCTGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGTATTCGAAcc                                                                                                                 <  7:1077591/151‑1 (MQ=255)
                                aGCTTTTTTAATGTTGCTGGGGTACGATGATTCGATCCTCGGAATGCCGTAATCAGAATCCTGTGCCTTACCGCTTGGCGATACCCCAACTGGGTTCACTTACAAGGTAAGCTGCTTGAATAAATTGGCTGGGGTACGAGGATTCGAAcc                                                                                                                 <  1:1198272/150‑1 (MQ=25)
                                 ggttttttTATGTGGCTGGGGGAGGATGATTAGAAACTCTGAATGACGCAATGAGTATACGGTGCCTTAGAGCTTGGCGATACCCCAAATCGGTGAACTTACAAGGTAAGGGTCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTc                                                                                                               <  7:483600/149‑1 (MQ=255)
                                   ttttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAGTGCCGGAATCAGATTCACGTGCCTAACATGTGTCCCATACCGCAGCGGTGTTCTGTTTCAAGGAAAGCGGCTTGAGTATAGTGGCTGGGGTGCGGGGGTTCgcggggcgg                                                                                                             >  3:923536/1‑143 (MQ=9)
                                    tttttATTGTGGCCGCGGGACGCGGCATCGAGCCTCGGAAGGACGGCATCAGACTCCCGAGCCTTACAGCTTGCCGTAACCCCAACTCGGTGAACTAACAACGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCgg                                                                                                             <  2:979152/150‑1 (MQ=255)
                                    tttttAATGTGGAGGGGGTACGATGATTCGATACTGTGAATGACGTAATCAGAATCCGTTGCATTACAGCTTGTCGATACCCAAACTGGGTTCACTTACAAGGTACGCTTCTTTAATAATTTGGCTGGGGTACGAGGATTCGAACCTctg                                                                                                             <  4:1438944/150‑3 (MQ=255)
                                          aTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGTCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCg                                                                                                      <  1:547050/151‑1 (MQ=255)
                                             tgGCTGGGGGACGAGGAATCGATACTCGGAATGCCGCAATCAGAATCCTGGGCCTTACAGCTTGGCGATACCCCAACTGGGTTCACGTACCAGGTAAGGGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGa                                                                                                    <  3:915544/150‑1 (MQ=255)
                                                 tGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGGTTTTTCAAGTGCAGCGGATTGTAGAAATAGGCAGAGGTACGTGGCTACGACCCTCgtcgatcctgtctccc                                                                                               >  4:536614/1‑136 (MQ=2)
                                                 gggggCACGAGGACACGATCCTCTGAATGCAGGAAGCAGACTCCCGGGCCTTACCGCTTGTATTAACCCAAACTTGGTTAACTTACAAGGTAGGCATCTTTTATAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAg                                                                                               <  4:1649979/150‑1 (MQ=12)
                                                       accaGGATTCGATCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCCACTGGGTGCACTTACTAGGTAAGCGTCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCg                                                                                         <  7:544604/148‑1 (MQ=255)
                                                       aCGATGATTCGAACCTCTGAATGCCGGAATCAGAATCCGTGGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCg                                                                                         <  7:1365771/151‑1 (MQ=255)
                                                       aCGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTCGGTGCAGTTTCAAGTGAAGCGGCTTTAATAAATTGTCTGGGGTTCGTGGCTTCGACGCTCGGAGTGCCggttgctggggccg                                                                                         >  7:704307/1‑139 (MQ=17)
                                                         gAGGATTCGACCCTCGGAATGCCGGAATCAGACACCGGTGCCTTCGCGCTTGCCGATACCCCAACTCGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                                       <  3:471606/151‑1 (MQ=37)
                                                         gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATATATTGGCTGGGGTACGAGGATTCGAACCTATGAAAGCCTCATTCAGAATcatgt                                                                                       >  1:1664933/1‑147 (MQ=25)
                                                              gtcgagactctGAATGCCGGAATCAGACTACAGCGCCTTACCCATTGCATCTACGCCCAATCGGAGCACTTACAACGTATGCGTCTTTAATAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                                                  <  7:726755/144‑1 (MQ=12)
                                                                      agggAATGCCTTAAGCTGTATCCGGCGCATTACCCCTTGCATGAACCCAAACTCGGAGCACTCTCCACGTACCCCGCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                                                          <  5:459726/149‑1 (MQ=2)
                                                                        ggAATGCCGTAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAAATTGGTTAACTTACAACGTAAGCTTCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                                        <  3:650047/151‑1 (MQ=25)
                                                                         gAATGCCTTCATCAGAATCCTTTGCCTTACCGCTTGGATTTACGCATACTTGGTTCACTTACTAGGTACGGTTCTTGAATAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                                                          <  2:1237297/148‑1 (MQ=9)
                                                                           aTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGGAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCTGATTCCCAAACCGGTGGCttcacgagtcgccat                                                                     >  7:1389895/1‑138 (MQ=37)
                                                                             gcctctaTCAGACTCCCGGGCCTTAGAGCTTGCATGTACGCCAACTGGGTTAAATCACTAGGAACGGGGCTTGTTTAAATTGGCTGGGGTACGAGGTTTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATa                                                                    <  5:811955/144‑1 (MQ=2)
                                                                               cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTAATTGCACTTACAAGTTAAGCGTCTTTAATATAATGGCTGAGGTACGAGGATTCGAAGCTCTTAATGCCTGAATCAGCATCATTTGCCTTAcagatatcagaaaaac                                                                 >  6:484813/1‑136 (MQ=16)
                                                                                gcaagcAGACTCCGGGGCCTTACAGATTGCCTAAACGCCAACTCGCTGCACTTACCAGGTAAGGTTCTTGAATAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATAcccc                                                                <  7:1345984/146‑1 (MQ=17)
                                                                                        actcctgggCCTTCCAGATTGGAGCTACCCCCACTCGGTGAACTTTCCAGGTATGCCGCTTTTATAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAtt                                                        <  4:785315/143‑1 (MQ=2)
                                                                                         aTCCGGTTCCTTACCGATTGTATATACCCAAACTTGGTTCACTTACAAGGTAAGCTTCTTTAATAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTg                                                       <  2:76783/151‑1 (MQ=18)
                                                                                         aTCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTg                                                       <  3:664733/151‑1 (MQ=255)
                                                                                           cccgggcattcccGCTTGGAGAAACCCCTACTCGCTAAACGAACCCGGAACGCCTCTTTATTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                                                     <  3:693463/140‑1 (MQ=9)
                                                                                           actttGCCTTACCGATTGTAGTTACCCCAACTCGGATCACTTTCAAGGTATGGTTCTTTTATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                                                     <  2:525397/147‑1 (MQ=17)
                                                                                              ttGCCTTACAGATTGTCGTTACTCATACTTGGTTCACTTACTAGGTAAGGTTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                                                     <  1:552748/147‑1 (MQ=18)
                                                                                              gcgCCTTCCCGATTGCCGCTACGCCAACACGGTCAAAGTTCCAGGTAAGGCGCTTTATTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGtttt                                                  <  3:1501662/149‑1 (MQ=14)
                                                                                                  attagagCTTGTAGAAACCCATACTTGGTCCAATTACTAGGTATGGTTCTTGAATAATCTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAAt                                              <  8:277082/145‑1 (MQ=17)
                                                                                                        gCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCg                                        >  3:1447764/1‑151 (MQ=255)
                                                                                                         cTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCg                                        >  4:332389/1‑150 (MQ=255)
                                                                                                                  aCCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCg                              >  8:1077119/1‑151 (MQ=255)
                                                                                                                   ccccAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCg                              >  1:1229294/1‑150 (MQ=255)
                                                                                                                    cccAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGAt                            >  7:926789/1‑151 (MQ=255)
                                                                                                                    cccAACTGGGAGCACGTTCACGGTAAGCCTCTTTAATAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGAt                            <  4:547099/151‑1 (MQ=21)
                                                                                                                     ccAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCcaataaccg                                                        <  3:1426120/122‑4 (MQ=21)
                                                                                                                       aaCTGGTTGCACTTACAAGGTAAGCGTGTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCACAAACCGGTGCCTTACCGCTTGGCGATACCCcaataaccg                                                        <  7:291389/120‑4 (MQ=18)
                                                                                                                            ggTGCACTTACAAGGTAAGCGTCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAg                    <  3:82768/151‑1 (MQ=255)
                                                                                                                                cACTTACAAGGTAATCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCACAAACCGGTGCCTTACCGCTTGGCGATAccc                                                                 <  1:278070/102‑1 (MQ=18)
                                                                                                                                cACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATAccc                                                                 >  7:277667/1‑102 (MQ=255)
                                                                                                                                 aCTTTCTAGGTAAGGGTCTTGTATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAAttt               <  2:1519707/151‑1 (MQ=255)
                                                                                                                                  atcaCAAGGTAAGGTTCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTtg              <  7:821616/148‑1 (MQ=255)
                                                                                                                                   ttACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTtgg             >  2:991225/1‑151 (MQ=255)
                                                                                                                                     aCAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTtggtg           >  7:1576691/1‑151 (MQ=255)
                                                                                                                                       acggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTg                                                                          <  2:770095/84‑1 (MQ=17)
                                                                                                                                          gaatgctgcTTTAATAATGTCGCTGGGGTACGAGGAATCCAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTac      <  6:1634053/143‑1 (MQ=21)
                                                                                                                                          gTACGCATCTTTATTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTac      <  3:1532761/151‑1 (MQ=255)
                                                                                                                                          gTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCtt                                                                           >  6:720645/1‑82 (MQ=255)
                                                                                                                                          gTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCGTACAGCTTGGCAATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTac      >  4:256336/1‑151 (MQ=255)
                                                                                                                                          ctAAGCCTCTTGTATAAAATGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCtt                                                                           <  4:721559/81‑1 (MQ=14)
                                                                                                                                           gacgccgcTTGTATAAAGGGGCGGGGGTACGCGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTacg     <  7:330081/144‑1 (MQ=25)
                                                                                                                                              cctgcTTGAATAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTacgacg  <  4:847773/147‑1 (MQ=255)
                                                                                                                                                 tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATAccc                                                                 >  3:185206/1‑85 (MQ=255)
                                                                                                                                                    tGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCACAATCCGGTGCCTTACCGCTTGGCGATACCCcaat                                                             <  7:1297165/86‑2 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
GAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACG  >  NC_000913/696385‑696677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑