BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,552,191	0	C	A	28.7%	873.1 / 131.9	470	intergenic (+200/+207)	fdnI/yddM	formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base C (96/232); new base A (110/22); total (215/255)
Fisher's exact test for biased strand distribution p-value = 6.42e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 475 total aligned reads displayed.

CATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCAT  >  NC_000913/1552041‑1552340

aatatTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTc                                                                                                                                                        <  2:600498/150‑1 (MQ=14)

 atatTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCa                                                                                                                                                       <  6:99457/151‑1 (MQ=31)

   atTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCaaa                                                                                                                                                     <  6:506886/151‑1 (MQ=33)

   atTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCaa                                                                                                                                                      <  3:578456/150‑1 (MQ=32)

   atTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCaa                                                                                                                                                      >  5:424301/1‑150 (MQ=32)

    ttGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAAt                                                                                                                                                    >  4:366847/1‑151 (MQ=33)

      gTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATg                                                                                                                                                   <  4:236055/150‑1 (MQ=33)

        aGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAACTGgcg                                                                                                                                                >  7:1120713/1‑151 (MQ=18)

          aCAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGGTTGTCATCAGTTTCAAATGgcgcg                                                                                                                                              >  4:79528/1‑150 (MQ=18)

          aCAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGAAAAGCGAAAAAGACAAAAAATCTTTTGTCATAAGTTTAAAAAGGCGCt                                                                                                                                              >  2:245916/1‑151 (MQ=11)

            aaCGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTGATCAGTTTCAAATGGCGCTG‑t                                                                                                                                           >  4:127717/1‑151 (MQ=25)

             aCGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑t                                                                                                                                           >  5:1050038/1‑150 (MQ=35)

               gTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTG‑Taa                                                                                                                                         >  6:1186823/1‑150 (MQ=21)

                    cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TATAAGGCg                                                                                                                                   >  5:1539227/1‑151 (MQ=37)

                     tttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGACCGTGTAGGCCTGATAAGCGTAGCGGATGATGCAAGGTTGCGTTTGTCATCAGTTTATAATGGCGCGG‑TATAAGGCGt                                                                                                                                  >  4:1176699/1‑151 (MQ=11)

                        gTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTGAAATGGCGCTG‑TAAAAGGCGTCAt                                                                                                                               >  4:1511460/1‑151 (MQ=255)

                        gTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCAt                                                                                                                               <  2:491323/151‑1 (MQ=255)

                        gTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCAt                                                                                                                               <  5:129536/151‑1 (MQ=255)

                        gTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCAt                                                                                                                               <  3:1225416/151‑1 (MQ=255)

                        gTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCAt                                                                                                                               <  6:365626/151‑1 (MQ=255)

                           cATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCTATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCAtttt                                                                                                                            <  2:142788/151‑1 (MQ=255)

                             tGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTG‑TAAAAGGAGTCATTTTc                                                                                                                           >  6:185895/1‑150 (MQ=255)

                              gCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCat                                                                                                                         <  4:65075/151‑1 (MQ=255)

                               ccGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTGAAATGGCGCTG‑TATAAGGCGTCATTTTCat                                                                                                                         >  5:550924/1‑150 (MQ=255)

                                  gATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTg                                                                                                                     <  3:641636/151‑1 (MQ=255)

                                  gATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTg                                                                                                                     <  3:473268/151‑1 (MQ=255)

                                  gATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGACAAAG‑TAAAAGCCTACATTTTCaaaat                                                                                                                      >  8:1637382/1‑146 (MQ=11)

                                    tGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTG‑TATAAGGAGGCATTTTGATATTGt                                                                                                                    >  5:946757/1‑150 (MQ=255)

                                     gcggcgTTAACGCGTTATCCGGCATGAAAACCCTTCAAATCCATTAGATTGCAGTGAACGTGTAGGCCTGATACGCGTAGCGCTTCAGGCAATGTTGCGTTTTTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTa                                                                                                                   <  4:1040032/150‑1 (MQ=17)

                                         cgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGGGGATCAGGCGATGTGGGGTTAGGTCTAATTTTATTAGGGCGCTG‑GATATGGCGTCTTTTTCTTTTTGTAGACa                                                                                                               >  4:339113/1‑150 (MQ=2)

                                         cgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGAGTTTGTCATCAGTTTAAAAAGAACCTG‑TAAAAGTCGTCATTTTCAAAATGTaacca                                                                                                               >  6:1455760/1‑146 (MQ=14)

                                          gTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TATAAGGCGGCATTTTCATATTGTAGACAAc                                                                                                             >  3:829302/1‑151 (MQ=255)

                                          gTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAAc                                                                                                             >  3:323576/1‑151 (MQ=255)

                                           tGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTG‑TAAAAGGCGTCATTTTAATATTGTAGACAACg                                                                                                            >  7:1062938/1‑151 (MQ=255)

                                            gAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTT‑TAAAATGGGTCATTTTAATATTGTAGACAACGt                                                                                                           >  2:1667815/1‑151 (MQ=14)

                                             aaCGCCTTATCCTGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGt                                                                                                           <  2:208485/150‑1 (MQ=255)

                                              aCGCCTTATCCTGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAg                                                                                                         <  1:270195/151‑1 (MQ=255)

                                              aCGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTG‑TATAAAGAGACATTTTAATATTGTATAAAACga                                                                                                           >  8:1026828/1‑148 (MQ=11)

                                                gCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTT‑TAAAAGGCGTCATTTTATTATTGTATACAACGTAGGc                                                                                                       >  5:785109/1‑151 (MQ=255)

                                                 gcTTATCCTGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTATCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCt                                                                                                      <  5:1652214/150‑1 (MQ=255)

                                                           cATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTG‑TAAAACGAGTCATTTTCATATTGTATACAACGTCGGCTTTGTTCATGc                                                                                            >  6:1277620/1‑151 (MQ=255)

                                                            attaaaaTCATTCAAATTCTATATATTGAATTGAAAGTGTAGTAATTATAATCGTAGCTCATCAGTCAATGTTGCTTTTGTCATCAGTTTAAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGcc                                                                                           <  2:1177062/148‑1 (MQ=18)

                                                            aTGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTG‑TAAAAGGAGTCATTTTAATATTGTAGAAAAAGTAGGCTTTGTTCATGcc                                                                                           >  2:357837/1‑151 (MQ=18)

                                                              gAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCg                                                                                          <  3:1419356/150‑1 (MQ=255)

                                                                aaaCCCTTCTAATCCAATAGATTGCAGAGACCGTGTAGGCATGATAAGCGTAGCGCAGCAGGCAATGTTGCTTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGAt                                                                                       <  8:847059/151‑1 (MQ=37)

                                                                  aCCCTTCAAATCCAATATATTTCATTGAAGGTGTAGGCCTGATAAGCGTTGGTGTTCTGTCAATGTTGCGTTTTTGTTCTGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATgc                                                                                     <  7:157737/151‑1 (MQ=18)

                                                                  aCCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTG‑TATAAGGAGTCATTTTCATATTGAAGACAACGTAGGCTTTGTTCATGCCGGATgc                                                                                     >  1:1056757/1‑151 (MQ=255)

                                                                       tCAAATCCAATAGATTGCAGTGAACGTGGAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATgcggc                                                                                  <  2:715514/149‑1 (MQ=255)

                                                                       tCAAATCCAATAGATTGAAGTTAACGTTAAGGCCTGATAAGCGTAGAGCATTAGTCAATCTTGCGTTTGTCATCAGTTTCATATTGCGATG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATgcggc                                                                                  <  8:725506/149‑1 (MQ=25)

                                                                        cAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATCGAGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGTCTTTGTTCATTCCGGATGAGGCGTg                                                                               >  2:909104/1‑151 (MQ=255)

                                                                         aaaTCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTG‑TATAAGGCGTCATTTTCTTATTGTCTCAAACGTAGGCTTTGTACATGCACGATgcgtcttg                                                                               >  5:673260/1‑145 (MQ=17)

                                                                         aaaTCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGGCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGa                                                                              <  7:501494/151‑1 (MQ=255)

                                                                          aaTCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGa                                                                              >  8:1207890/1‑150 (MQ=255)

                                                                          aaTCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTG‑TAAAAGGGGTCATTTTGATATTGTAGACAACGTAGGCTTTGTTAATGCCGGATGCGGCGTGa                                                                              >  5:1380987/1‑150 (MQ=255)

                                                                             ccAATAGATTGCAGTGAACGTTTAGTCATGATAACCGTATCTAATCAGTCAATGTTGATTTTGTCATCAGTTTAAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGc                                                                          <  8:62704/151‑1 (MQ=18)

                                                                              cAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCCTAAGTTTCAAATTCAGCTG‑TAAAATGAGAATTTTAGATATTGTATACAAGGTACTCTTTGTTAAATCATCATGCGGCGTGAACGcg                                                                         >  4:952568/1‑150 (MQ=9)

                                                                               aaTAGATTGCACTGAACGTCTAGGCCTGATAAGCCTAGCGCATCAGGCAATGTAGCGTTTCTCATCACTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCt                                                                        <  5:358157/151‑1 (MQ=21)

                                                                                aTAGATTGCCGTGCACGTGTAGGCCTGATAAGGGTAGGGCATGATGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCtt                                                                       <  1:673390/151‑1 (MQ=21)

                                                                                 tAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGATCATAAGAGAATCTTGGGTTTGTAATCAGTTTCAAATAGAGATG‑AAAAAGGGGGGATGTTGATATTGTAGAAAAAGTAGGCTTTGTTCATGCCGGCTGCGGCGTGAACGCCttg                                                                      >  8:1455200/1‑150 (MQ=16)

                                                                                    aTTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCGTCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTG‑TAAAAGGAGTCATTTTCATATTGAATACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTTAACGCCGTagac                                                                   >  6:819699/1‑148 (MQ=21)

                                                                                    aTTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTT‑TAAAAAGAGNCATTTTCATATTGTAGACAAAGTAGGCTTTGTTCATTCACGATCAGGCTTGAACGCATTATcc                                                                   >  1:853266/1‑151 (MQ=17)

                                                                                      tGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCg                                                                  <  3:913789/150‑1 (MQ=255)

                                                                                       gCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                <  8:1122081/151‑1 (MQ=255)

                                                                                          gTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATg                                                             >  4:477894/1‑151 (MQ=255)

                                                                                              aCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTGAAATGGCGCTG‑TAAAAGGCGTCATTTTGATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCGTTATCCGGCATGaaa                                                          >  7:324303/1‑150 (MQ=255)

                                                                                                gtgtAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTAATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAAcc                                                       >  1:115796/1‑151 (MQ=255)

                                                                                                 tgtAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATACGGCATCAAAAccc                                                      >  8:1357194/1‑151 (MQ=255)

                                                                                                  gtAGGCCTGATAAGCGTAGCGCATCAGGCTATGTTTCGTTTGTCATCAGTTTCAAATGTCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAAcc                                                       <  8:180932/149‑1 (MQ=255)

                                                                                                   tAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGACTTATCCGGCATGAAAACCCtt                                                    >  3:240716/1‑151 (MQ=255)

                                                                                                    aGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCtt                                                    <  8:517072/150‑1 (MQ=255)

                                                                                                     ggCCTGATACGCGTAGCGCATCAGGCAATGTTGCGTGTGTCATCAGGTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCa                                                  <  5:738237/151‑1 (MQ=255)

                                                                                                       ccTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGGGTCATTTTGATATTGTAGACAAAGTAGGCTTTGTTCATGCCGGATGCGGCGTGATCGCCTGATCCGGCATCACAACCCTTGaa                                                 >  3:1582071/1‑150 (MQ=21)

                                                                                                        cTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAAt                                               >  3:403300/1‑151 (MQ=255)

                                                                                                         tGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATc                                              >  7:868680/1‑151 (MQ=255)

                                                                                                         tGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTG‑TAAAAGGCGGCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATCCCGGATGCGGCGTGAACGCCGTATCCGGCATGAAAACCCTTCaact                                               >  7:1449492/1‑148 (MQ=255)

                                                                                                          gATAAGCGTAGCGCATCAGGCAATGTTGCGTCTCTCATCAGATTCAAATGGCGCTG‑TAAAAGGCGGCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATcc                                             <  7:676602/151‑1 (MQ=255)

                                                                                                             aaGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAAt                                          <  4:1562758/151‑1 (MQ=255)

                                                                                                              atcGTAGCGCATCAGGCTATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATa                                         <  1:1273387/149‑1 (MQ=255)

                                                                                                              aGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATa                                         <  8:402986/151‑1 (MQ=255)

                                                                                                              aGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTG‑TAAAAGGCGTCATTTTAATATTGTAGACAAAGTAGGCTTTGTTCATGCCGGATGAGGCGTGAACGCATTATCCGGCATGAAAACACTTCAAATCCAAt                                          >  1:501336/1‑150 (MQ=18)

                                                                                                                 gTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAAt                                          >  4:1443114/1‑147 (MQ=255)

                                                                                                                  tAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGAtt                                     >  7:1332093/1‑151 (MQ=255)

                                                                                                                   aGCGCATCATGTAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCTTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTg                                    <  7:1179416/151‑1 (MQ=255)

                                                                                                                   aGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTG‑TAAAAGGAGTCATTTTAATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAAAGCCTTATACGGCATGAAAACCCTTCAAATCCAATAGAttt                                    >  6:1167440/1‑150 (MQ=255)

                                                                                                                        aTCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTg                               >  2:1282656/1‑151 (MQ=255)

                                                                                                                          gagtcAATGTTTCGTTTGTCATAAGTTTCAAATGGCGCTG‑TATAAGGCGTCATTTTCATATTGTAGTCTACGTAGGCTTTGTTCATGCCGGATGGGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGGAGTGat                             <  2:1515592/147‑2 (MQ=14)

                                                                                                                          cAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGaa                             <  6:64832/151‑1 (MQ=255)

                                                                                                                          cAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGACTAATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGa                              >  6:1594405/1‑150 (MQ=255)

                                                                                                                          cAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTAATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCAAGAAAACCCTTCAAATCCAATAGATTGCAGTGa                              >  7:607690/1‑150 (MQ=255)

                                                                                                                              cAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTG‑TAAAAGGCGTCATTTTAATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAAAGACTTATACGGCATGAAAACCCTTCAAATACAATAGATTGAAGTGAACgtg                         >  1:1639522/1‑151 (MQ=17)

                                                                                                                                  gtttcttttGGCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAg                      <  3:1039196/144‑1 (MQ=255)

                                                                                                                                  gTTGCGTTTCTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAgg                     <  3:1368409/151‑1 (MQ=255)

                                                                                                                                      gctttGTCATCAGTTTCAAATGGCGCTG‑TAAAATTCGTTATTTTCATATTGTAGTCTATGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTg                 <  1:370163/149‑1 (MQ=14)

                                                                                                                                      cGTTTGTTATTAGTTTCAAATTG‑GCTGTTAATACTCTTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTg                 <  2:1058216/151‑1 (MQ=11)

                                                                                                                                         ttGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGAt               >  1:1036972/1‑150 (MQ=255)

                                                                                                                                             cataaGGTTAAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGc           <  5:234638/150‑1 (MQ=9)

                                                                                                                                              atcaGGGTCAACTGGCGCTG‑TAACAGGCGGCATTTTCATATTGTATACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTTAACGCCTTATCGGTCATTAAAAGCCTGCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGt         <  5:1454399/151‑1 (MQ=11)

                                                                                                                                               tcaGTTTAAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCg          <  7:370440/149‑1 (MQ=21)

                                                                                                                                               taaGTTTAAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGt         <  6:1029686/148‑1 (MQ=17)

                                                                                                                                                    ttCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAgcgc    >  8:342276/1‑150 (MQ=33)

CATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTG‑TAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCAT  >  NC_000913/1552041‑1552340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`