BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,270,151	0	A	C	23.2%	209.7 / 23.0	192	intergenic (+549/+474)	yhaC/garK	pentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand): ref base A (93/46); new base C (42/0); total (144/48)
Fisher's exact test for biased strand distribution p-value = 9.72e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

GCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACC  >  NC_000913/3270012‑3270301

                  ggcccgccccAGCCATTCATCATTCAGCCCCTCCAAAACCAAAAACCCGCCGAAGCTGTTTTTTACGTAAAACAGGTTAACCTGACCGATAAGCCGCCTTCTTTTGGGTATAGCGGCGTTGACAGGCATTCATCTTTCTGCCCCTCCaaaa                                                                                                                              <  5:1635832/142‑1 (MQ=11)

                               cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCTTGGCAAGGCAATAAAGGTTAAGCGCCCCCAAAACCAAAAAcccccc                                                                                                                 >  4:494110/1‑151 (MQ=11)

                                attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCATTGTGCTCCGTCAAAAACAAAAACccgc                                                                                                                  >  5:391776/1‑149 (MQ=11)

                                 ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTGGTGGCCAGTCCTTCATCTTTGGGGCGCGGCAAAACCAAAAACccgccgc                                                                                                               >  3:43476/1‑150 (MQ=11)

                                 ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGAAGTCAATCAAGAGTTTGCACCGACAAAACCAAAAACccgccgc                                                                                                               >  6:373249/1‑150 (MQ=11)

                                  actaataacgggcccTCAACAAGCTCAAACCCGCCGGATCGTGTTTTTACGTACACCAGGTCAAACTGACCGATACGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATGTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑aa                                                                                                             <  7:906424/139‑1 (MQ=11)

                                    gaataactggcacTCAAAAAGCTGAAACCCGCCGTATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAg                                                                                                            <  2:664641/138‑1 (MQ=11)

                                    atttttatctcacTCAAAAATCTAAAACCCGCCGTATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑c                                                                                                          <  2:501308/139‑1 (MQ=11)

                                      cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGGCATTCATGGTTGTGCGCCGGCAAAATCAAAAACCCGCCG‑CAG‑C‑gg                                                                                                       >  5:879282/1‑151 (MQ=11)

                                       tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCAATCATGTTTGTGCCCCGGCAAAAGCAAAAACCCGCCG‑CAG‑g‑ggg                                                                                                      >  3:1220882/1‑151 (MQ=11)

                                       tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTGTTGTGGATAGAGTCGAGTACAGTCCATCATATTTAACCACCGACAAAAGCAAAAACccgcggcagagcg                                                                                                         >  8:589094/1‑142 (MQ=11)

                                       tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGG‑TTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGGATAGCGTAGTGGACAGGCCATCAAGTTTGTCCCGCGACAAAACCAAAAACCCGCGG‑AAggacgg                                                                                                       >  8:1172122/1‑146 (MQ=11)

                                        ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTAGTGGCCAGTCCTTCATGTTTGAGCCCGGGCAAAAGCAAAAAACCGCGG‑CAG‑C‑GGGt                                                                                                     >  1:1236555/1‑151 (MQ=11)

                                           tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAGCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTAGAGCGTCGGGGCGAGGCCTGCATGGTTTTGCCCCGGCAAAACCAAAAACccggggcagtggggtgt                                                                                                     >  5:1417346/1‑137 (MQ=2)

                                           tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCCATCATGGTTAGGCGCCGCCAAAACCAAAAAACCGCCG‑AAG‑C‑CGGgttt                                                                                                  >  3:306089/1‑151 (MQ=11)

                                           tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGGGTATAGAGACGAGTAAACTCATACAAAATTAAGCACCAGCAAACTCAAAAACCCGCCG‑AAG‑cacgatat                                                                                                   >  1:1063286/1‑144 (MQ=2)

                                             ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAGAGTCAATCAAAGTTTGTCGCCGACAAAAACAAAAACCCGGCG‑AAgacggcgtgt                                                                                                   >  2:1162445/1‑141 (MQ=11)

                                              cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCATGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTAc                                                                                               >  7:1544163/1‑151 (MQ=25)

                                                acgctattgctatatcccGCCGTATCGGTTTTTTACGTAAATCAGGTTAAACTGTCCTATAAGGCGGTTTCTTTTGGGTATAGCGTCGTTGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGt                                                                                             <  2:894488/136‑1 (MQ=11)

                                                  ccAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGTCGTCGACACTCAATCAACTATAGGCCCCACCAAAAGCAAAAACCCCCCG‑CAG‑cccggttagaacta                                                                                             >  1:1352292/1‑137 (MQ=11)

                                                   cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCCTTCATCTTTGGGCGCCGCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTaa                                                                                           >  4:773549/1‑150 (MQ=11)

                                                    aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCGTTGTGCCCCGGCAAAAGCAAAAACCCGCCG‑CAG‑C‑GGGTTTTTACGGAAAt                                                                                         >  3:569151/1‑151 (MQ=11)

                                                    aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCATTGATCGTTGTGCGCCGGCAAAAACAAAAACCCGCCG‑AAG‑G‑GGGGTTTTTCggtaat                                                                                         >  3:505433/1‑146 (MQ=11)

                                                       aGCACAAACCCGCCGCACCGGGTTATTACGTAAACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAg                                                                                      <  7:1340395/151‑1 (MQ=255)

                                                        gCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACAAGGTGAAACTGACCGATAAGACGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCATTCATAATTAACGCACTACAAAAGCAAAAACACCCCG‑AAG‑G‑GGGTTTTTAcaaaaaaaa                                                                                       >  2:1685472/1‑141 (MQ=11)

                                                          aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCAGTGATGTTTGTGCCCCGGCAAAAACAAAAACCCGCCG‑AAG‑C‑GGGTTTTTTCGTAAATCACGTg                                                                                   >  3:379338/1‑151 (MQ=11)

                                                           aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGTCAGTCCTTCATGGTTGGGCGCCAGCAAAACCCAAAACCCGGCGCAGG‑C‑GGGTTTTT‑CGGTAATCAGGTgg                                                                                  >  5:725533/1‑150 (MQ=11)

                                                            aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAGAGGCATTCATGGGTGTGCACCACCAAAACCAAAAACCCGCCG‑CAG‑CTGGGTTTTTACTTaatcagctcc                                                                                   >  5:1544827/1‑142 (MQ=11)

                                                            aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCCTTGATGTTTCTGCCCCGGCAAAAGCAAAAACCCGCCG‑CAG‑C‑GGGTGTTTTCGTAAATCAGGTGa                                                                                  >  5:83941/1‑150 (MQ=11)

                                                              aCCCGCCGTATCGTTTTTTTACGTACAGCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAGAGTCATTCATCTTTCTGCCACTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAAc                                                                               <  2:395133/151‑1 (MQ=11)

                                                                ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTACTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTGTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGGTTTTTCGGAAATGAGGGGAAACtt                                                                             >  5:1451780/1‑150 (MQ=11)

                                                                 cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCAATCATCATTTGGCCCCGGCAAAACCAAAAACCCGCCG‑CAGCC‑CGGTTTTAACG‑AAATCAGCGGAAACttg                                                                            >  5:636667/1‑149 (MQ=11)

                                                                   ccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCAATCATATTTGGGCCCCTGCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACCTAAATCAGGTGCAACTTAcc                                                                          >  5:1201087/1‑151 (MQ=11)

                                                                   ccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCAAAATTCTGCCCCCGCAAAACCAAAAACCCCCGG‑AAG‑A‑GGGTGTGTACGGAAATAATGAGAAAATTAcc                                                                          >  7:984528/1‑151 (MQ=11)

                                                                   ccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGCGTATAGAGTCGTGGCAAGACATTAATCGTTAGGCCCCACCAAAACCAAAAAACCGCCC‑AAG‑C‑GGGTATTTAAGTAAATCAAGGGAAACTgcac                                                                          >  7:707143/1‑148 (MQ=11)

                                                                   ccgAACCGTGTTATTACGTAAACCAGGTAACACTGAGCGATAACGCGCTTTGTTTTTGGTATAGTGTCGGCGACAGTCCGTCATCTTTCTGCCACTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGAcc                                                                          <  5:98731/151‑1 (MQ=11)

                                                                     tcagttttttattACGTAAATGAGGTTAATGTTACCTATAAGCGTGTTTCTTTTGTTTATAGTGTCTTGGAGAGTGATTCATCTTTCTGCTCCTCCAAAATTAAATACGCGCGT‑AAG‑A‑TGGTTTTTACGTAAATCAGGTGAAACTGAc                                                                           <  6:216730/137‑1 (MQ=255)

                                                                     gccccGGTTTTTTACGTACATCAGGGCAAACTGACCGATAAGCCTCTTTCTTTTGTGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGa                                                                        <  3:1063457/147‑1 (MQ=11)

                                                                     gAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGTGTATAGAGTATAAGAAAGTCAATCAAAATTAAACACCAACAAAAACAAAAACCCCCAT‑CAG‑A‑AGATGTTTACATAAAACAGGTGaaaaatacagt                                                                        >  6:54258/1‑143 (MQ=2)

                                                                      attcGGGTTTTTACGTAATCTAGGTTAAAGTGTCCTATATTTCGCTTTATTTTGGTTTTAGGTTCTTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGGAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGAt                                                                       <  2:379604/148‑1 (MQ=11)

                                                                      aaGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATGTTTGAGCCCCGGCAAAAACAAAAACCCGCCG‑CAG‑C‑CGGGTTTTAACTAAATGAGGTGAAACTGCCCGAt                                                                       >  6:727471/1‑151 (MQ=11)

                                                                       aGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATTCATCTTTATGCGCCAACAAAAACAAAAACCCCCCG‑AAG‑C‑GCGTTTTTAAGTAAATCAggggcaagagccccta                                                                       >  6:1558704/1‑136 (MQ=11)

                                                                        gCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTAGTGGAAAGTCAATAAAAATTAAACACCAACAAAAACAAAAACCCGCAG‑CAG‑A‑CCGTTTATAACTAAATCAACTCAAAATTACCGATa                                                                      >  1:448392/1‑150 (MQ=2)

                                                                         cGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCAAGTCATTCATAATTGAGCCCCAACAAAAACAAAAACCCGCCG‑CAG‑A‑ACGGTTTTACGGAAATCAGGTGAAACttccccata                                                                      >  6:548242/1‑141 (MQ=11)

                                                                          gggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGAGAGTCAATCATCTTTAGGCGCCAACAAAAGCAAAAACCCGCGG‑AAG‑C‑GGGGTTTTACGTAAATGAGGTGAAAATTACCGATGAGc                                                                   >  8:193758/1‑151 (MQ=11)

                                                                           ggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATATTTCAGCCCCAACAAAACCAAAAAACCGCCG‑AAG‑C‑CGGTGTTTTCGTAAATCAGGGGAAAATGACCGATAATcc                                                                  >  4:1173583/1‑151 (MQ=11)

                                                                            gTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTGTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCg                                                                 >  4:736271/1‑151 (MQ=25)

                                                                            gTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAATCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTTCGTAAATCAGGTGAAACTGACCGATAAGCCg                                                                 >  4:279974/1‑151 (MQ=255)

                                                                                  aCGTACATCAGGTTAAACTTACTGATAAGCCGCTTTCTTTTTTGTATAGTTTCGTGTACAGTCATTCATCTTTCTGCCCTTCTAAAATCTAAAACCCGCCG‑AAT‑C‑GGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTc                                                            <  2:670050/150‑1 (MQ=11)

                                                                                   cGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCATAATTGAGCCCCGACAAAACCAAAAACCCGCCG‑CAG‑C‑GGGTGGTTAAGGAAATCACGGGCAAATTCGCGATAAGCCGGGTTCTg                                                          >  6:85638/1‑151 (MQ=9)

                                                                                     tAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTAAGCCCCGGCAAAAACAAAAACCCGCCG‑CAGAC‑GGGTTTTAAGGT‑AATCAGGTGAAACTGGCCCGTAAGCCGGGtggtgtg                                                        >  6:1453773/1‑145 (MQ=14)

                                                                                         ccAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTAAGCACCAACAAAAGCAAAAACCCGCCG‑AAG‑A‑CCGGTATTAAGTAAATCACGTGAAAGTTACCCATAATCCGGGTTgtgtagagg                                                    >  6:800809/1‑146 (MQ=9)

                                                                                            ggTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTATGCCCCTGCAAAAGCAAAAAACCGCCG‑AAG‑C‑CGGTTTTTACGTAAATCAGGTGAAACTTACCGATAAGCCCGGTTCTGTCGTgggc                                                  >  7:1152575/1‑148 (MQ=14)

                                                                                             gtgtattattatgttAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAGAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACa                                                 <  6:1099714/136‑1 (MQ=14)

                                                                                             gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACACTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACa                                                 <  1:836072/150‑1 (MQ=255)

                                                                                             gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCAGTGAATAAAATTTGTGCCCCACCAAAAACAAAAAACCCCCC‑CAC‑C‑GGGGTTTTAAGTAAATCACGTGCAACTTCCCCAGAAGCCGGGTTCTGTCGTggggcc                                                >  3:189236/1‑147 (MQ=9)

                                                                                             gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCGTTATGCCCCTACAAAAGCAAAAACCCGCCG‑CAG‑C‑GGGGTTTTACGTTAATCACGTGAAACTTACCGATAAGCCGGGTTCTGTGGTGGGCac                                                >  7:242634/1‑150 (MQ=14)

                                                                                              taaaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCCAGGCAATCATCTGTGAGGCGCACCAAAAGGAAAAACCCGCAC‑AAG‑C‑CGCGTTTTACGTTAAACACGTGTAAATGAACCAAAACCCGGGTTCGGtgggggggcgg                                               >  3:264103/3‑141 (MQ=2)

                                                                                              tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTGCGCCCCTCCAAAACCAAAAACCAGCCG‑AAG‑C‑CCGGTGTTAAGTAAATCACGTGCAACTGCCCGACAAGCCGGGTGCTGtgggggcg                                                  >  5:1429302/1‑141 (MQ=11)

                                                                                               gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTACAAAAGCAAAAACCCGCCG‑CAG‑C‑GGGTTTTTAAGTAAATCAGGTGCAAATGCCCGATAAGCCGGGTTCTGTAGTggcccgtg                                              >  5:248122/1‑145 (MQ=14)

                                                                                                aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCCAGGCATTCATATTTGTGCCCCGGCAAAAGCAAAAACCCGCCG‑AAG‑G‑GGGTTTTTAACGAAAACAGGTGCAAATTCCCGATAAGCCGGGTTGTGTTGTggttaggct                                             >  7:423735/1‑144 (MQ=2)

                                                                                                    tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTGTTTACGTAAATCAGGTGAAACTGACCGATAAGACGGGTTCTGTCGTGGACAGtcattc                                          >  6:776964/1‑150 (MQ=255)

                                                                                                     caCCGATAAGCCTTTTTTTTTTTTTTATATCGTCGTCGACAGTTATTTAAATTTTTGCCTATACTATATCAATAACCGTCGG‑TAT‑T‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattc                                          <  6:1256497/148‑1 (MQ=12)

                                                                                                      aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATATTTAAGCCCCAACAAAAACAAAAACCCGCAG‑AAG‑A‑CCGTTTTTAAGTAAAACAGGTGAAAATTACCCAAAAGCATGGTTCTTTAGTGTAAAGtcaatgat                                        >  6:1523674/1‑145 (MQ=2)

                                                                                                      aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATc                                       >  1:630675/1‑151 (MQ=255)

                                                                                                      aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTAATTGATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCG‑AAG‑C‑GGGTTTTTACGTAAATAAGGTGAAAATGCCGCATAAGACGGGTTGTGTGGTGGACAGTCATTCATc                                       >  1:1223525/1‑151 (MQ=17)

                                                                                                      aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTAATTCATATTTGTGCCCCGCCAAAAGCAAAAACCCCCCG‑AAG‑C‑GGGGGTTTTCGTAAATAAGGTGAAAATGAGCGATAAGCCCGGTTCTGTCGTGGCCAGTCATTGATc                                       >  2:1066528/1‑151 (MQ=17)

                                                                                                       ccGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTACTCTTTAGGCCCCTACAAAAGAAAAAACCCGCCG‑AAG‑A‑GGGGTTTTAAGTAAATCAGGTTAAAATGACCGATAAGCAGGGTTCTTTCGTTGCCAGtcattactct                                      >  5:558575/1‑146 (MQ=14)

                                                                                                       actataatattctttatttTGTTTATATAGTCGTCGATATTCATATATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCt                                      <  2:804103/140‑1 (MQ=17)

                                                                                                        cgccacgtcccatcctttTGGGTATAGCGTCGTCGACAGTCATTCATCTTTCTGCCCCTCCAAAACTAAAAACCCGACT‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGTTTCTGTCGTGGACAGTCATTCATCTa                                     <  3:814874/137‑1 (MQ=17)

                                                                                                        cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑CAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCt                                      >  4:1111866/1‑150 (MQ=255)

                                                                                                         gATAAGCCGCTTTCTTTTGGGTATAGTGTTGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACAAGCCG‑AAG‑C‑CGGTTTTTACGTAAAACAGCTGAAACTGACCGATAAGCCGCTTTCTGTGGTGGAAAgagaataataaa                                     >  1:358225/1‑139 (MQ=1)

                                                                                                         gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTAATTCATATTTCTACCCCTCCAAAATCAAAAACACGCCG‑AAG‑C‑GGCTTTTTACGTAAATCAGGTGAAACAGACCGATAACCCGGGTTCTGTCGTGGCCAGTCATTCAACt                                      >  1:675672/1‑149 (MQ=17)

                                                                                                          aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAgg                                   >  4:155786/1‑151 (MQ=255)

                                                                                                            aaGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATAAGGTGAAAATGACCGATAAGCCGGGTTCTGTAGTGGACAGTCATTAATATAGGcc                                 >  8:1619726/1‑151 (MQ=17)

                                                                                                               ccGCTTTCTTTTGTTTATAGTGTTTTTGACAGTCATTCCACTTTCTGCAACTCTATACCCAAAAACGAGTCT‑CTT‑C‑CGGTTTTTAATTACATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGc                              <  8:1381589/151‑1 (MQ=16)

                                                                                                               ccGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAACAAAAACCCCCCG‑AAG‑C‑CGGTTTTTAAGTAAAACACGTGAAACAGACCGAAAAGCCGCGTTCTGTTGGGGATAGacatttagcgaggaaagc                              >  2:428695/1‑138 (MQ=1)

                                                                                                                 gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCCAAAAGCCGGGTTCTGTGGTGGGCAGTAATTCATCTAGGCCAGCa                             >  8:1260561/1‑150 (MQ=17)

                                                                                                                  ctttctttTGGGTATAGCGTCGTGGCCAGTGATTGAACTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAAACAGGTGAAACTGACAGATAACCCGGGTTCTGTCGTGGGCATTCATTCAAGAAGGCCAGCaa                            >  1:106864/1‑150 (MQ=17)

                                                                                                                   tttctttTGGGTATAGCGTCGTGGCCAGTCATTAATCTTTCTGCCCCTCCAAAAGCAAAAACCCACCG‑AAG‑C‑GGGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTGGGGGACAGTCATTCATCTAGGCCAGCAATc                          >  7:627207/1‑151 (MQ=18)

                                                                                                                    ttctttTGGGTATAGCGTCGTGGCCAGTGATTCATCTTTGTGCCCCCCCAAAAACAAAAAAACCCCG‑CAG‑C‑GGGGTTTTTAGTAAATAAGGTGAAACTGAACGATAAGCCGGGGTGTGTGGTGGCCCGGCAATCATATAGGCCCGCAATCg                         >  4:1497346/1‑151 (MQ=255)

                                                                                                                      ctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGCCAGTCATTCATCTAGGCCAGCAATCGcg                       >  7:604775/1‑150 (MQ=255)

                                                                                                                            ggtatatttttttttaCATTCATTCATCTTTCTGCACCTCCAAAATAAAAAACCCGCTT‑AAG‑C‑TTGTTTTTACGTAAATCAGGTGAATCTGACGGATAAGGGGGTTTGTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTgg                 <  8:389767/136‑1 (MQ=18)

                                                                                                                            ggTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGCCAGTCATTCATCTAGGCCAGCAATCGCTCACTgg                 >  7:12544/1‑151 (MQ=255)

                                                                                                                             gTATAGGGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAACAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACAGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGc                <  3:753002/151‑1 (MQ=255)

                                                                                                                              tataGCGTCGTGGACAGTGATTCATATTTCTGACCCGGCAAAAGCAAAAACCCGCCC‑AAG‑C‑GGGTTTTTACGTAAATAAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAATGATTAATCTAGGCCAGAAATAGCGCaatttc                >  2:249301/1‑145 (MQ=17)

                                                                                                                              tataGCGTCGTGGACAGTCTTTAATCTTTCTGCCCATCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGCTTTTTACGTAAAAAAGGTGAAACAGGCCGATAAGACGGGTTCTGTCGGTGTCAGTCAGTCATATTTGCCAGGAATCGCTACCTgggg               >  6:1297393/1‑149 (MQ=9)

                                                                                                                                taGCGTCGTGGACAGTCATTCATATTTGTGCCCCAACAAAAGCAAAAACACCCCG‑AAG‑C‑GGGTTTTTAACTAAATAATGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTGATTGATCAAGGCCCGCAAACGGTCACAGGc                >  6:301219/1‑148 (MQ=255)

                                                                                                                                  gggtcgtGGACAGTCATTCATCTTTCTGCCCCTCCAAAACCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAACCTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAag           <  7:357714/149‑1 (MQ=255)

                                                                                                                                   cgtcgtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagc          >  7:1099447/1‑151 (MQ=255)

                                                                                                                                    gtcgtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATTGCTCACTGGCTCAagc          >  2:1463286/1‑150 (MQ=255)

                                                                                                                                    gtcgtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagc          >  4:80069/1‑150 (MQ=255)

                                                                                                                                    gtcgtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagc          >  5:1086407/1‑150 (MQ=255)

                                                                                                                                      tgtgtACAGTCATTCATCTTTCTGCCAATACAAAAGTAAAATCCCGCCG‑AAG‑G‑TGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGTTTGTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagcag        <  1:177851/149‑1 (MQ=21)

                                                                                                                                      cgtTGACAGTCAATCAGCTTTCTGCCGCTCCATAGGCATCAGACGGGAG‑ATG‑A‑GCGGTTTTACGTACAGCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagcag        <  3:1567401/150‑1 (MQ=255)

                                                                                                                                       gtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAAGCAGGTGAAACTGACCGATAAGCCGGGTTCTGTGGGGGAGAGTGTCTGCGCGAGGCAAGCAAGCGCTCACtccgtcaacacca       >  4:1515661/1‑138 (MQ=11)

                                                                                                                                       gtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCCCTCACTGGCTCACgccgcc      >  7:1426238/1‑151 (MQ=255)

                                                                                                                                       gtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACACTCATTCATCTAGGCCAGCAATCTCTCACTGTCTCAAGCAGcc      >  2:1293848/1‑151 (MQ=255)

                                                                                                                                         ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCt     >  8:86979/1‑150 (MQ=255)

                                                                                                                                           aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAcc  >  3:658096/1‑151 (MQ=255)

GCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACC  >  NC_000913/3270012‑3270301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`