BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,392,457	0	G	T	23.4%	355.0 / 15.5	226	intergenic (+22/‑15)	glyV/glyX	tRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand): ref base G (47/104); new base T (4/42); total (53/173)
Fisher's exact test for biased strand distribution p-value = 1.88e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

CCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGA  >  NC_000913/4392309‑4392605

cccGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAg                                                                                                                                                      <  4:634838/149‑1 (MQ=255)

cccGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGc                                                                                                                                                     <  7:1080515/150‑1 (MQ=255)

 ccGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGcac                                                                                                                                                   <  7:634102/151‑1 (MQ=255)

 ccGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCATGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGcac                                                                                                                                                   <  5:454334/151‑1 (MQ=255)

  cGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGcaca                                                                                                                                                  <  2:767607/151‑1 (MQ=255)

   gTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAg                                                                                                                                                 <  2:700025/151‑1 (MQ=255)

     aaCGAGGCTGATATGCTAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTGAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGGCGGGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGac                                                                                                                                               <  8:425101/151‑1 (MQ=18)

     aaCGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGac                                                                                                                                               >  6:1510854/1‑151 (MQ=255)

       cgacgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGacca                                                                                                                                             <  1:1039734/151‑1 (MQ=255)

       cgacgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTAGAAAAGATCAGCGTCGAGAATTCAAATCTAGTATCCAAATCAAAAAATATAAAAGTACTGAAAATcaaaaaaa                                                                                                                                              >  6:248810/1‑144 (MQ=255)

           gCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCa                                                                                                                                         <  1:907567/151‑1 (MQ=255)

             aGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAg                                                                                                                                       <  5:591965/151‑1 (MQ=255)

             aGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAg                                                                                                                                       <  1:1396832/151‑1 (MQ=255)

              gAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGc                                                                                                                                      <  2:990580/151‑1 (MQ=255)

               aaaTGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCg                                                                                                                                     <  4:1512264/151‑1 (MQ=255)

               aaaTGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCg                                                                                                                                     <  4:35745/151‑1 (MQ=255)

                aaTGCGTAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCgg                                                                                                                                    <  1:1231865/151‑1 (MQ=255)

                    cGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAAt                                                                                                                                <  4:1269180/151‑1 (MQ=255)

                    cGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAAt                                                                                                                                <  5:64024/151‑1 (MQ=255)

                    cGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAAt                                                                                                                                <  8:1045421/151‑1 (MQ=255)

                     gAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATa                                                                                                                               <  1:688023/151‑1 (MQ=255)

                     gAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATa                                                                                                                               <  7:1570346/151‑1 (MQ=255)

                     gAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATa                                                                                                                               <  3:1405474/151‑1 (MQ=255)

                      aaaaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATCGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAg                                                                                                                              <  1:28032/151‑1 (MQ=255)

                       aaattccttaatataaaTTAACTAGTACGCGGGAATAGCTAAGTTGTTATAGCACTACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTT‑TAAAGTTCTGTAAGTCACAGACCACCCAAGCGGGAATAGCt                                                                                                                            <  1:650816/137‑1 (MQ=1)

                       aaaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACATACCACCCAAGCGGGAATAGc                                                                                                                             <  2:1013024/151‑1 (MQ=255)

                       aaaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTAGAGAGTTAGAGTCTAGTTTAGCGCTACAAAATTTGAAAATTGCTTCAAAGCACAGACCACCCAATCAGTAATat                                                                                                                              >  2:1044467/1‑149 (MQ=18)

                          ttACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAATTGATTAAAAACAAAGACACACCAACCTGGAATAgaac                                                                                                                           >  2:31359/1‑147 (MQ=17)

                          ttACCAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTGCAAAATTTGAAAATTGATTAAAAGAACAAACCACACAACCGGGACTAGCta                                                                                                                           >  8:464576/1‑149 (MQ=17)

                             cGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGtt                                                                                                                       <  3:1436828/151‑1 (MQ=255)

                               aaaGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTgg                                                                                                                     <  4:172689/151‑1 (MQ=255)

                                   cAAAATTAAGTAGTACGCTGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTaga                                                                                                                 <  5:1410842/151‑1 (MQ=255)

                                    aaaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTagag                                                                                                                <  4:1161309/151‑1 (MQ=255)

                                    aaaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTagag                                                                                                                <  7:377316/151‑1 (MQ=255)

                                         tAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCAGGACCTTTCGTAGTGAGTTGTCGCTAGTTCGAGGCTCGTTTCCCGCTCCAAAATTTGAAAAGTTCTGCATACCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACg                                                                                                            <  8:324671/150‑1 (MQ=14)

                                           tgttttactctggAATATCTCAGTTGTTAGTGCTGGAGCTTTGTTAGTTCGTTGTCGCTATTTGGTGTTTCGTTTACCTCTCCTAAATTTGAAAAGTTCTGCACATCACAGACGACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGAc                                                                                                          <  8:24648/139‑1 (MQ=9)

                                                taatatggAATATATCAGTTGTTAGAGCTGGAGCTTTCCAATTGAGGGGTCGCTAGTTCGAGTTTCGTTTCCGTCTCAAAAATTTGAAAAGTTCTTAAAATAACAGACCAAGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGc                                                                                                     <  8:1113271/144‑1 (MQ=2)

                                                 aCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCa                                                                                                   <  5:1497051/151‑1 (MQ=39)

                                                     gggAATAGCTCAGTTGTTAGCGCTCGAGCTTCCCAATTGCGGGGTCGCGATTTCGAGGCTCGGTTCCGGCTCAAAAATTTGAAAAGTTCTTCAAATCAAAGACGACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGt                                                                                               <  6:935933/151‑1 (MQ=14)

                                                        aaTAGCTCAGTTTTTAGAGGACGACCTTTCGAAGGTCTGTGTCGCTAGATCGACGCGCGTTACCCGCTCAAAAATTTGAAAAGTGCTTCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                                                                                            <  4:1112292/151‑1 (MQ=14)

                                                         aaaGCGCAGGTGGTCGCGCACTCCATAGCCAACGTCGGGGCCGCTACTGCGCGTCCCGTAACCCGGCACAAAA‑TTGAAAAGTCCTGAACCCCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgggg                                                                                          <  3:743551/149‑1 (MQ=2)

                                                           aGCTGATTTGCTAGAGCACGACCTTGCAAAGGTGGGGGTCTCGAGATCGACTCTCTGTTCCGGCTCCAAAATTTGAAAATTGCTTCAAATAAAAGAACAACCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGt                                                                                         <  3:1045493/151‑1 (MQ=14)

                                                           aGCTCAGTTTGTGGGGGCGGACCTTGCCAAGGAGGGGGGGGCTAGTGCGCCTCGCGTAACCC‑CCCCCAAATGTGAAAAGTCCTCTACCCCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc                                                                                        <  7:1215640/151‑1 (MQ=2)

                                                           aGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGt                                                                                         <  4:931408/151‑1 (MQ=38)

                                                           aGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGt                                                                                         <  3:649961/151‑1 (MQ=38)

                                                            gCTCAGTTGGTAGAGGTCGACCTTGCCAAGGTCGGTGTCGCTAGTTCGTGTCTCGTTTCCCGCTCCAAAATATGAAAAGTGCTGCAAACCACAGACGACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc                                                                                        <  4:566948/151‑1 (MQ=14)

                                                            gCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc                                                                                        <  3:204675/151‑1 (MQ=38)

                                                            gCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc                                                                                        <  3:31415/151‑1 (MQ=38)

                                                               cAGTTGGTAGCGCACGAGCTTGCCAATCTCGGTGTCGCGAGTTCGAGGCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAATCACAGACCACGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgc                                                                                      <  1:54978/150‑1 (MQ=14)

                                                                    tgTAGAGCACGACCTTGCCAAGGTCGGGGTCGCTAGTTCGAGGCTCGTTTACCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGtt                                                                                 <  7:1619233/149‑1 (MQ=255)

                                                                         agCTCGACCTTCCCAAGCTCGGGGTCGATAGTTCGAGTGTCCTTTCACTCTCCAAAATTTTAAAAGTGCTTCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtc                                                                           <  8:1210966/151‑1 (MQ=14)

                                                                          gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGGCTCGGTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct                                                                          <  4:1050288/151‑1 (MQ=255)

                                                                             cGAGCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGGTTCCCGCTCCAAAATTTGAAAAGTGCTCCAAACCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGt                                                                       <  4:1303998/151‑1 (MQ=255)

                                                                                ccTTGCCAAGGGCGGGGTCGCGAGTTCGAGGCTCGTTACCCGCTCCAAAATTTGAAAAGTCCTGCAAAGCACATACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                    <  6:676095/151‑1 (MQ=255)

                                                                                   tGCCAAGGGCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTccc                                                                  <  5:1247077/150‑1 (MQ=38)

                                                                                     ccAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAATTGATTAAAAGCACAGACCACCCAACCGGAAAAACATAAGTTGTTAGAAAACGAACATGCGAATGTAGGGGAAGAGAGTTAGAGTATCttttaaaaa                                                                >  6:1279782/1‑145 (MQ=2)

                                                                                     ccAAGGTATGGGTCGTTAGTTCGACGCTCGTTACCGGCTCAAAAATTTGAAAAGTGCTTAAAATCACAGAAGACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCt                                                               <  8:1237146/151‑1 (MQ=11)

                                                                                       catggcGGGGTCGCGTGATCGAGTGTCGTTTCCGCCTCCAAAATTCGAAAAGTGCTGCATATCACAGACCTCCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTcc                                                             <  2:572432/146‑1 (MQ=14)

                                                                                       aactgatttgTCGCTATTTCGCGGATCGGTTACCGCTCAAAAATTCGAAAATTCCTCCACCCCAAATACCACGCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTcc                                                             <  5:659703/142‑1 (MQ=2)

                                                                                         ggtatgtgTCGCGATTTCGAGTCTCGTTTACCTCTCAAAAATTTGAAAAGTGCTTAATATCACAGACGACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaa                                                           <  8:1342716/144‑1 (MQ=11)

                                                                                         gggcgtggTCGCGACTTCGAGTCTCGTTTCCGCCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACGACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaa                                                           <  7:1410763/145‑1 (MQ=14)

                                                                                          gTCGGGGTCGCGAGTTGGAGTCTCGTTTCCCTCTCCAAAATTTGAAAAGTGCTTCAAATCACATACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaa                                                           <  8:706322/150‑1 (MQ=255)

                                                                                            cGGGGTCGCTAGTTCCAGGCTCGTTTCCCGCTCAAAAATTAGAAAAGTGCTTCACACAACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaaa                                                         <  5:1309719/150‑1 (MQ=14)

                                                                                               tgTCGCTATTTCGAGGATCGTTTCCCGCTCATAAATTTGAAAATTTCTTCACATCAAATACCTCCGAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAAttt                                                      <  2:223966/149‑1 (MQ=14)

                                                                                                  cgcgAGTTCGAGTCTCGTTTCCAGCTCCAAAATATGAAATG‑GATTTAATAGACAGACCACCCAAGCGGGAAAATCTCAGTTTTTATAGAAACACCATGACAATGTAAGGGTCGGGAGTTCGAGTCACCATTCGCGCACCTAAATGTTaaat                                                 >  6:1149245/1‑150 (MQ=2)

                                                                                                  cgcgAGATCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTCCTCCAAAGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaa                                                   <  2:1590152/150‑1 (MQ=255)

                                                                                                     gAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGATGTAATGAACAGACCACCCAAGAGGGAATATCTCAGTTTGTAGAGAAAGACCTAAACAAGGTAGGGCTCGCGACTACCAATCAAGATTCACGATCCAAAATATaaaataa                                               >  2:1155535/1‑147 (MQ=2)

                                                                                                      aGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTg                                               <  2:660318/150‑1 (MQ=255)

                                                                                                         tggactctcGTTACCCGCTCAAAAATTTGAAAAGTTCTTCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTg                                            <  1:219894/145‑1 (MQ=17)

                                                                                                         tcgacggtcctttCCCGCTCAAAAATTTGAAAAGTGCTCCACCGAACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTg                                            <  4:1100524/140‑1 (MQ=17)

                                                                                                         tCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAACCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTg                                            <  7:474265/150‑1 (MQ=255)

                                                                                                          ggcggctcGTTACCCGCTCCAAAATTTGAAAAGTGCGCCACATCACTGACGACCGAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTa                                          <  5:766944/146‑1 (MQ=17)

                                                                                                           gAGTCTCGTTTCCCTCTCCAAAATTTGAAAAGTTCTCCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTaa                                         <  1:1373591/151‑1 (MQ=255)

                                                                                                                    ttaccccTCCAAAATTTGAAAAGTGCTGCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGa                                 <  6:873455/144‑1 (MQ=255)

                                                                                                                    ttCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCGCCAAAATATTAAAGTGCTGTAAgggccagcc                                >  3:75405/1‑144 (MQ=255)

                                                                                                                     tCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTTTGAACGAGCttaaagccccacagc                               >  7:659670/1‑137 (MQ=14)

                                                                                                                     tCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGGCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGac                                <  5:520475/150‑1 (MQ=255)

                                                                                                                     tCCCCCTCCAAAATTTGAAAATTCCTCCACATAACATACCACGCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGac                                <  6:1396320/150‑1 (MQ=17)

                                                                                                                        cccTCCAAAATTTGAAAAGTGCTTAACATCACATACGAAGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGaccacc                            <  1:1627530/149‑1 (MQ=17)

                                                                                                                         gCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTATGAAAGAAATGTAATGAACACACCAccc                           >  6:1410073/1‑151 (MQ=14)

                                                                                                                           gccAAAATTTGAAAAGTCCTGCATATAACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCaa                         <  6:1633879/150‑1 (MQ=255)

                                                                                                                            ccAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTTTGAACGTGCTGTAAGGGCCAGAcccccccgg                        >  7:1126447/1‑148 (MQ=17)

                                                                                                                             cAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTTTGAAAGTGATGTAAGGGACAGAGCCCCCacgg                       >  3:1565489/1‑148 (MQ=17)

                                                                                                                              aaaaTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGGGCTGTAAGCGACACACCAcccccccg                      >  5:851788/1‑146 (MQ=17)

                                                                                                                               aaaTTTGAAAATTGCTGCAACCAAAATACCAAGGAAGCGGGAATAGCTCAGTTGGTAGAGCACTACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCgg                     <  4:1529381/151‑1 (MQ=14)

                                                                                                                               aaaTTTGAAAAGTCCTGCAACGCACATACGACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTGCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCgg                     <  5:1339148/151‑1 (MQ=17)

                                                                                                                                 aTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTGTGATAGTGATGTAATGGACAGACCCCCCCCGCgggt                   >  5:990772/1‑150 (MQ=14)

                                                                                                                                 aTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAATAGATCTAAAGCACAgccgacaccagcagg                    >  2:477958/1‑136 (MQ=14)

                                                                                                                                   ttGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTTTAATGGCCAGACCGcccccgggggtat                 >  4:716145/1‑141 (MQ=17)

                                                                                                                                   ttGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCCCCCACGGGGGAAt                 >  7:957141/1‑151 (MQ=255)

                                                                                                                                      aaaaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATTAAATAACTGTAATAAACACACCACCCAAAAGGGAAAAGc              >  6:419165/1‑151 (MQ=14)

                                                                                                                                       aaaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGGCTCGTTTCCCGCTCCAAAATTTGAACGTGCTCGAAGGGACACACCCCCCCCGCGCGAAtttgt             >  3:476143/1‑147 (MQ=14)

                                                                                                                                        aaGTGCTGCAAAGCACAGCCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGCGTCTCGTTTCCCGCTCCAAAATGTGCTAGTGCTGTAAGGGCCAGAGCCCCCCAGCgcgcttatggc            >  5:92860/1‑141 (MQ=14)

                                                                                                                                         cccgcgccAACGCCCAGCCCACCCACCCGGGAATAGCGCAGTGGGAAGCCCACGACCTTGCCAAGTTGGGGGTCGCGATTTCGAGGCGCTTTGCCCGCTCCAAAATTTCAAAGTGCTGAAACGCACAGACCACCCAAGCGGGAATAGCTCa           <  7:684388/144‑1 (MQ=2)

                                                                                                                                             ctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGCAAGAGCTGTAAGGCACACACCCCCCAAGGGGGAAAAGCTCAGTTg       >  7:817680/1‑151 (MQ=17)

                                                                                                                                                cAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATTAAAGTTATTTAATGGACAGACCACCGCAGGGGGattttgggagtttttt    >  6:319589/1‑136 (MQ=14)

                                                                                                                                                 aaaGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTCAAGTGCTGAAATCGACACAGCCCCCACCCGGGAATTTCTCAGtttttgt   >  4:800181/1‑146 (MQ=14)

                                                                                                                                                 aaaGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTGAAAGGGATGTAAGGGACAGACCCCCGACGCGGGAATATCTGAGTTgttct   >  5:904067/1‑147 (MQ=17)

                                                                                                                                                 aaaGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTGAAAGAGATTAAATGACCAGACCACCCACGCGGGAATAGGTCGGtttttat   >  4:294974/1‑146 (MQ=14)

                                                                                                                                                  aaGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATTAAAGTGATTTAATGTCCAGACCGACCAAGCGGGAAtttgtgagtttttttt  >  3:792039/1‑136 (MQ=14)

CCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGA  >  NC_000913/4392309‑4392605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`