Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4320TG23.7% 894.4 / 77.0 420intergenic (+73/‑86)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (88/228);  new base G (97/1);  total (189/231)
Fisher's exact test for biased strand distribution p-value = 4.62e-41
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 434 total aligned reads displayed.

TCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTC  >  NC_000913/422283‑422580
                                                                                                                                                     |                                                                                                                                                    
    gTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGtccgccgcc                                                                                                                                                 >  6:1266557/1‑151 (MQ=255)
      ggATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGACATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTcccc                                                                                                                                               <  6:214156/151‑1 (MQ=255)
           gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCAGCCCCCGTCGTCCTACCCCAAat                                                                                                                                          >  3:1420120/1‑151 (MQ=255)
            ttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAat                                                                                                                                          >  6:1489276/1‑150 (MQ=255)
              gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATc                                                                                                                                       <  1:1788970/151‑1 (MQ=255)
              gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATc                                                                                                                                       <  2:841789/151‑1 (MQ=255)
              gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATc                                                                                                                                       >  1:1124836/1‑151 (MQ=255)
               tgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATATcc                                                                                                                                      >  5:595678/1‑151 (MQ=255)
                gCTGTGGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCt                                                                                                                                     <  8:624522/151‑1 (MQ=255)
                 cTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCTCCGCCCCCAAAAATCCt                                                                                                                                     >  2:1221677/1‑150 (MQ=255)
                    ttGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGACCTCCTCCCCCAAATATCCTTCat                                                                                                                                 >  8:171246/1‑150 (MQ=255)
                     tGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCACCCCCAAATATCCTTc                                                                                                                                   >  4:561382/1‑148 (MQ=255)
                      gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                               <  7:730945/151‑1 (MQ=255)
                      gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                               <  1:1216556/151‑1 (MQ=255)
                       aTTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatg                                                                                                                              <  3:1006648/151‑1 (MQ=255)
                         tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGCCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCACCACCCCCAAATATCCTTAagaaga                                                                                                                             >  2:1656982/1‑150 (MQ=255)
                          ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCGGCCGCCCCCGAAGATCCTTCAggggcgg                                                                                                                           >  8:1123777/1‑145 (MQ=255)
                           gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGatgatgc                                                                                                                          >  6:548435/1‑150 (MQ=255)
                              ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCGGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTg                                                                                                                       <  3:543231/151‑1 (MQ=255)
                              ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGt                                                                                                                        <  6:325380/150‑1 (MQ=255)
                                  gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGGTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                                                    <  2:374391/150‑1 (MQ=255)
                                    ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCt                                                                                                                  <  2:1111291/150‑1 (MQ=255)
                                    ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTg                                                                                                                 >  7:427206/1‑151 (MQ=255)
                                      aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGCCGCCCCCCAAGCTCCTTGAGCTGATGAGTGctgctggc                                                                                                               >  5:1682117/1‑149 (MQ=255)
                                       aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAt                                                                                                              <  6:730514/151‑1 (MQ=255)
                                        aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCGCGTCGGGCGCCCCCATATATCCTTCAGATGATGAGTggaggggcctt                                                                                                             >  1:552618/1‑141 (MQ=255)
                                          gccgccACGATCGTCAGGTGGCTGAAAACCAGTAATCCTTGCGTTGTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAg                                                                                                           <  3:793109/151‑1 (MQ=255)
                                             gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCGCCCCCAAAAAACCTTCAGAAGATGAGTGGGCCGGCAGTAGGCt                                                                                                        >  3:972889/1‑151 (MQ=255)
                                                aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCGGCCGGCCGCACATATCCTTCAGATGATGAGGGAGGCTGGATTAggggatg                                                                                                     >  3:1842429/1‑146 (MQ=255)
                                                aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                     <  4:1153927/151‑1 (MQ=255)
                                                aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                     <  4:1784673/151‑1 (MQ=255)
                                                 cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAGATCCATCACATGAAGAGTGATCCTGCCGTAGGCTATgg                                                                                                    >  1:333730/1‑151 (MQ=255)
                                                  gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCGGCCCCCCAATCTCCGGCAGATGATGAGGGCTACTGGATGAGGCGAGGGc                                                                                                   >  5:1030448/1‑151 (MQ=255)
                                                     cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                                                 <  6:1354934/150‑1 (MQ=255)
                                                      gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCGGGCCCCCAAAAAGCAGCAGATGATGACTGATGCTGCAGGAGGCTATgggtggg                                                                                               >  8:589087/1‑146 (MQ=255)
                                                      gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATCGTTCAGATGATGAGTGCTCGTGGAGTAGGGTATGGCTAgg                                                                                               >  5:1428762/1‑151 (MQ=255)
                                                      gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCGTGAGATGATGAGTGCTCCTGCATTAGGCTATGGCAAgg                                                                                               >  5:542735/1‑151 (MQ=255)
                                                        cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCATGAGGAAATCCTGATACGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATTGCAAGGTg                                                                                             <  5:1168066/151‑1 (MQ=255)
                                                        cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTg                                                                                             <  3:1545352/151‑1 (MQ=255)
                                                          gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCCAATATCCTTGAGATGATGAGTGATCCTGCAGTAGGCTAGGGCATggggtt                                                                                           >  4:240683/1‑147 (MQ=255)
                                                          gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCTCCTCGGCCCCCCAATATCCTTCAGATGATGAGTGATCCTGCATTAGGGTATGGCTAggggtt                                                                                           >  7:196541/1‑147 (MQ=255)
                                                            tggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCCTTAGGGGATGGCAAGGTGATc                                                                                          >  7:1604848/1‑150 (MQ=255)
                                                            gggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCa                                                                                         <  8:1298482/150‑1 (MQ=255)
                                                             ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCGGCGCCAACTATCCTTAAGATGATGAGTGCTGCGGCATGAGGGGATGGCAAGGGGAGCa                                                                                         >  2:667311/1‑150 (MQ=255)
                                                                tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGGAGTAGGCTATGGCAAGGAGCTCAGGtt                                                                                     >  8:28928/1‑151 (MQ=255)
                                                                   aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGGCGCCCCCAAATAGCCGTCACAGGATGAGTGATACTGGATTAGGGTTTGGCAAGGTGATCAGATTTtc                                                                                  >  5:1290108/1‑151 (MQ=255)
                                                                   aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtc                                                                                  <  1:696696/151‑1 (MQ=255)
                                                                        aGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATCCTTCAGATGAGGAGGGATCCTGCATTAGGGGATGGGAAGGGGATGAGATTTTAATaac                                                                             >  8:1757750/1‑151 (MQ=255)
                                                                         gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcaca                                                                            <  8:790246/151‑1 (MQ=255)
                                                                           aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgg                                                                          <  3:726753/151‑1 (MQ=255)
                                                                              ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCATGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                        <  5:996002/150‑1 (MQ=255)
                                                                                ttGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCCAAGATCGTTCAGAGGAGGAGTGATCCGGCATTAGGCTATGGGCAGGTGAGGAGATGTTGATCACAGGGGGAt                                                                     >  4:1681708/1‑151 (MQ=255)
                                                                                 tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCGGCCCCACAGATCCTTCCGATGAGGAGTGATGCTGCATTAGGGTTTGGCAGGGTGATGAGATGTTCATCACAGGGGAAtt                                                                    >  5:1570821/1‑151 (MQ=255)
                                                                                  gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAtt                                                                    <  7:327999/150‑1 (MQ=255)
                                                                                  gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATATCCTTCAGATGAGGGGTGGTGCTGCAGTAGGCTATGGGGCGGTGGTCAGGTTTTCTTCACAGGGGTAttt                                                                   >  6:1184611/1‑150 (MQ=255)
                                                                                    gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATAAGATTTTGATAACAGGGGAATTatt                                                                 >  2:1845875/1‑150 (MQ=255)
                                                                                    gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCC‑CCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatga                                                                <  1:1022389/151‑1 (MQ=255)
                                                                                         gTAAGCCGGTTCAGGAGGAAAGCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgatg                                                              <  8:1021808/149‑1 (MQ=255)
                                                                                          tAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCTTTAGGCTTTGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGAttttt                                                           >  3:1070385/1‑150 (MQ=255)
                                                                                              ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTGAGATGATGAGTGATCCTGGATTATGCTATGGGAAGGTGATGAGATTTTGATAACAGGGGAATTTTGATGTTTAAtt                                                       >  3:308279/1‑150 (MQ=255)
                                                                                               cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGc                                                      <  1:1400093/151‑1 (MQ=255)
                                                                                                  ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCGCGTCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGGATTAGGCTATGGGAAGGTGATGAGGTTTTGATCACAGGGGGAGTTTGATGTTAAATGCATg                                                   >  8:543587/1‑151 (MQ=255)
                                                                                                    cAGGAGGATATCCTGATCCTGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCTTATATCCTTCAGATGATGAGTGTTGGTGCATTAGGCTATGGCAAGGTGATCAGATTTTCCTCACAGGGGAATTATGATGTTAAATGCATGGc                                                 <  2:1768986/151‑1 (MQ=255)
                                                                                                    cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGGTATGGCTAGGTGATCAGATTTTGATCACAGGGGAATTATTATGTTTAATTCATgtc                                                 >  6:1778662/1‑149 (MQ=255)
                                                                                                    cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATAACAGGGGAATTATTATGTTAAATTCATGGc                                                 >  8:662074/1‑151 (MQ=255)
                                                                                                     aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCa                                                <  5:1261693/151‑1 (MQ=255)
                                                                                                       gaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGGATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAcc                                              <  4:330500/151‑1 (MQ=255)
                                                                                                       gaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGGTATGGCAACGTGATCAGATTTTCATGACAGGGGAAGTATGATGTTAAATGCATGGCaac                                              >  7:1037201/1‑149 (MQ=255)
                                                                                                        aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCGGGGCGCCTCCCCCCAATATCCAGCAGTTGATGAGGGGAGCGGCGGTAGGCGTTGGCTAGGTGATGAGAGTTTGATGTCAGGGGATTTATGATGTTTTATGcgtcggcccg                                             >  4:872866/1‑142 (MQ=255)
                                                                                                           aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAATGTGATCAGATTTTCATGACAGGGGAATTATGATGTTAAATGCATGGCACCTGcc                                          <  2:1046020/151‑1 (MQ=255)
                                                                                                           aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGcc                                          <  7:1407415/151‑1 (MQ=255)
                                                                                                             aTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGTGTGATCCGGCAGTATGCTGTGGGCAGGTGATCAGATTTTAATCACAGGGGACTTATTATGTTAAATTCAGGGCACCGGCggg                                        >  3:1355960/1‑151 (MQ=255)
                                                                                                             aTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATACTTCAGATGATGAGTGATCCTGCAGTAGGCTATGGGAAGGTGATCAGATTTTCATAACACGGGAATTATGAAGTTAAATGCATGGCAAATGCCgg                                        >  5:1808146/1‑151 (MQ=255)
                                                                                                              tCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAAAATCCTTCAGATGATGAGTGATCATGCATTAGGCTATGGCAAGGTGATCTGATTTTCATAACAGGGGAGTTATGTTGTTTAATTCATGGCACCTGCCggg                                       >  7:1220212/1‑150 (MQ=255)
                                                                                                              tCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCGCCGCCCCCAAATATCCATCAGAGGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAGCAGATTTTCATAACAGGGGAAGTATGATGTTATATTAATGGCACCTGACGGt                                       >  2:1518436/1‑151 (MQ=255)
                                                                                                               ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGt                                       <  8:1198938/150‑1 (MQ=255)
                                                                                                                    tCCGGCTATTCCGAAAGTTATCCGCGGCCGGCCGCGGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTTGGGCAAGGTGATCAGATTTTGATCACAGGGGAATTTTGATGTTTAATGATTTGCACCTGCGGGTggcgcc                                 >  3:1211449/1‑146 (MQ=255)
                                                                                                                    tCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTGCGCCCCCCACAATCCTTCAGATGTTGAGTGATCCTGCATTAGGGTATTGCAAGGTGAGCAGATTTTGATCTCAGGTGTATTATGATTTTAAATGCATTGCACCTGCAGGTGccccc                                 >  1:867235/1‑151 (MQ=255)
                                                                                                                     ccGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGCAATTATGATGTTAAATGCATGGCACCTGCCGGTGccccc                                 <  2:606722/150‑1 (MQ=255)
                                                                                                                      cGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATAACAGGGGAATTATGATGTTAAATTCATGGCACCTGCCGGTGGCCCCAt                               >  6:579823/1‑151 (MQ=255)
                                                                                                                       ggCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGCTCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGCATTATGATTTTAAATGAATGGCACCTGCCGGTGCCCCCAtt                              >  6:448025/1‑151 (MQ=255)
                                                                                                                       ggCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAAAAACCGGCAGATGATGAGTGATACTGCATTAGGCTATTGCAAGGTGATCAGATTTTAATAAAAGGTGAATTATTATGTTAAATGCATGGAAACTGCCGGTGCCCCCagt                              >  5:42940/1‑149 (MQ=255)
                                                                                                                        gCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAttt                             <  8:1456246/151‑1 (MQ=255)
                                                                                                                           aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                          <  6:977153/151‑1 (MQ=255)
                                                                                                                             tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                         <  1:1574574/150‑1 (MQ=255)
                                                                                                                             tCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCAGTGAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATGAGATTTTAATCACAGGGGAATTATGATGTTAAATTAATGGCACCTGCCGGGGCCCCCATTTGTTa                         >  4:1487831/1‑150 (MQ=255)
                                                                                                                                 aacggtATCCGCCCCCGGCCTGCTCCCCCAAATATCGTTCAGATGATGAGTGATCCTGCATTAGGCTATGGGAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaa                    <  5:842293/146‑1 (MQ=255)
                                                                                                                                 aaaGGTATCCTCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATCGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGATAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaa                    <  3:43867/151‑1 (MQ=255)
                                                                                                                                  aaGTTATCCGCCGCCGTCCTCCTCCCCCAAATATCCTTCAGATGATTAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaa                   <  4:1233471/151‑1 (MQ=255)
                                                                                                                                  aaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaa                   <  7:1355870/151‑1 (MQ=255)
                                                                                                                                     ttATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagc                 <  8:1596067/150‑1 (MQ=255)
                                                                                                                                     ttATCCGCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAaaaagc                 >  1:814590/1‑150 (MQ=255)
                                                                                                                                      tATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagca                <  1:24800/150‑1 (MQ=255)
                                                                                                                                           gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa               >  7:810080/1‑146 (MQ=255)
                                                                                                                                             ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa         >  7:452440/1‑150 (MQ=255)
                                                                                                                                             ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa         >  8:43305/1‑150 (MQ=255)
                                                                                                                                               ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt      >  5:753202/1‑151 (MQ=255)
                                                                                                                                               ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt      >  7:147824/1‑151 (MQ=255)
                                                                                                                                                   cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCt   <  2:500624/150‑1 (MQ=255)
                                                                                                                                                    ctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc  >  5:964801/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTC  >  NC_000913/422283‑422580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑