Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4350TG23.1% 904.8 / 70.9 420intergenic (+76/‑83)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (89/230);  new base G (96/0);  total (189/231)
Fisher's exact test for biased strand distribution p-value = 1.79e-42
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 442 total aligned reads displayed.

GTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTAC  >  NC_000913/422287‑422585
                                                                                                                                                    |                                                                                                                                                      
gTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGtccgccgcc                                                                                                                                                      >  6:1266557/1‑151 (MQ=255)
  ggATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGACATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTcccc                                                                                                                                                    <  6:214156/151‑1 (MQ=255)
     ttgttgTGCTGTTGATTGGCTGGATGTTAAAACGCCGCCACGATCGTCAGCTTGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCa                                                                                                                                                  <  8:611528/150‑1 (MQ=255)
      tgttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCaa                                                                                                                                                 >  2:777728/1‑150 (MQ=255)
        ttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAat                                                                                                                                               >  6:1489276/1‑150 (MQ=255)
          gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATc                                                                                                                                            >  1:1124836/1‑151 (MQ=255)
            gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACCATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCCGTTCAGGAGGAAATCCTGATCCGGCTATTCCCAAAGTTATCCCCCCCCCTCCGCCAGCCCCaaaaaaac                                                                                                                                           >  8:1749421/1‑145 (MQ=255)
             cTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCTCCGCCCCCAAAAATCCt                                                                                                                                          >  2:1221677/1‑150 (MQ=255)
             cTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAAAATACtt                                                                                                                                         >  3:384191/1‑151 (MQ=255)
                  gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                                    <  1:1216556/151‑1 (MQ=255)
                     tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCACATAGCCtgctgagga                                                                                                                                  >  1:673171/1‑142 (MQ=255)
                     tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGCCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCACCACCCCCAAATATCCTTAagaaga                                                                                                                                  >  2:1656982/1‑150 (MQ=255)
                       gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTGCTCCTCCCCCAAATATCCTTAAGatgatgt                                                                                                                               >  5:585701/1‑150 (MQ=255)
                       gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGatgatgc                                                                                                                               >  6:548435/1‑150 (MQ=255)
                          ggATGTTTAAACGCCGCCACGATCGTCATATGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTTATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGt                                                                                                                             <  5:177612/150‑1 (MQ=255)
                          ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGt                                                                                                                             <  8:838644/150‑1 (MQ=255)
                            aTGTTTAAACGCCGCCACGATCGTCAGCTTGCTGAAAACCAGTAATCCTTGTGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGa                                                                                                                           <  1:321140/150‑1 (MQ=255)
                              gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                                                         <  7:749400/150‑1 (MQ=255)
                              gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGGTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                                                         <  2:374391/150‑1 (MQ=255)
                                ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCt                                                                                                                       <  2:1111291/150‑1 (MQ=255)
                                  aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATGCGCGCGCGGGCGCCGGCCCCAAAAATAAATAAGATCATGACTGATGCTgg                                                                                                                     >  5:242601/1‑149 (MQ=255)
                                  aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCa                                                                                                                    <  2:502848/151‑1 (MQ=255)
                                   aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAt                                                                                                                   <  6:730514/151‑1 (MQ=255)
                                   aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAt                                                                                                                   <  3:1719272/151‑1 (MQ=255)
                                    aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCGCGTCGGGCGCCCCCATATATCCTTCAGATGATGAGTggaggggcctt                                                                                                                  >  1:552618/1‑141 (MQ=255)
                                    aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAtt                                                                                                                  <  4:1588510/151‑1 (MQ=255)
                                     cgccgcCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTa                                                                                                                 <  5:1035969/151‑1 (MQ=255)
                                       ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                               <  8:45079/151‑1 (MQ=255)
                                         gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCCAATATCCTTGAGAGGATGAGTGGTCCGGCATTATGCt                                                                                                             >  5:1413558/1‑151 (MQ=255)
                                         gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTAGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTATGCt                                                                                                             >  2:1776726/1‑151 (MQ=255)
                                            aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                          <  4:1784673/151‑1 (MQ=255)
                                             cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGGTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGAGCCTGCATTAGGCTATgg                                                                                                         <  7:1399021/151‑1 (MQ=255)
                                               aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                                       <  3:1008012/151‑1 (MQ=255)
                                               aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                                       <  7:111033/151‑1 (MQ=255)
                                               aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCGCCGCCCCCCAATATCCGTAAGATGATGAGTGATGCTGCATTAGGGGATGGc                                                                                                        >  3:514481/1‑150 (MQ=255)
                                                 cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                                                      <  6:1354934/150‑1 (MQ=255)
                                                  gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAgg                                                                                                    <  7:1004613/151‑1 (MQ=255)
                                                     aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCCGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAAAATCAGTCAGATTATGAGGGATACAGCAAGAGGCTATGGCAAGGTg                                                                                                  >  8:1649891/1‑150 (MQ=255)
                                                        tggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGGTCCGGGAGGAGGGGATGGGAAGGTGATc                                                                                               >  3:1401666/1‑150 (MQ=255)
                                                            tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGGCGGCGGGCGCCCGCCCCGATCCTGCCGAGGAGGAGTGCTGCTGCGTTAGGCGAGGGCAAGGTGCTCAGAtt                                                                                          >  7:592139/1‑151 (MQ=255)
                                                            tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTAAGCTGATGAGTGATCCTGCATTAGGCTATGGGAAGGTgcgccgagt                                                                                          >  6:338292/1‑143 (MQ=255)
                                                             gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAt                                                                                           <  4:237452/149‑1 (MQ=255)
                                                             gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAt                                                                                           <  3:1491690/149‑1 (MQ=255)
                                                               aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtc                                                                                       <  7:393164/151‑1 (MQ=255)
                                                               aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtc                                                                                       <  5:1464038/151‑1 (MQ=255)
                                                               aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtc                                                                                       >  5:922508/1‑150 (MQ=255)
                                                                aaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtca                                                                                      <  7:912538/151‑1 (MQ=255)
                                                                          ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGGCGGCGGGCTGGCGCCACTATCCGGCAGATGAGGAGTGGTGCTGCATTAGGCGTTGGGAAGGTGATCAGAGTTTCATCAGAggggt                                                                            >  7:272121/1‑150 (MQ=255)
                                                                          ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCGGCGCCCCCAAAAAACCATCGGAAGAGCAGAGAACCAGGCTGAGGCTAAGGGAAGGTGGTCAGATTTTCATCACAgggg                                                                             >  3:87245/1‑150 (MQ=255)
                                                                          ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCATGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                             <  5:996002/150‑1 (MQ=255)
                                                                              gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATATCCTTCAGATGAGGGGTGGTGCTGCAGTAGGCTATGGGGCGGTGGTCAGGTTTTCTTCACAGGGGTAttt                                                                        >  6:1184611/1‑150 (MQ=255)
                                                                              gCGTTCTGTAAGCCGGTTCAGGAGGAAAGCCTGATCCGGCTATTCCGAACGTTAGCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTa                                                                        <  3:1074489/151‑1 (MQ=255)
                                                                                gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATAAGATTTTGATAACAGGGGAATTatt                                                                      >  2:1845875/1‑150 (MQ=255)
                                                                                gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCC‑CCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatga                                                                     <  1:1022389/151‑1 (MQ=255)
                                                                                       aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGGGGGGGGGCGCCCCCCAATATGCTTGAGATGATGAGTGATGGTGGAGTTGGGTATGGGTAGGTGATGAGATTTTGATCGAAGGGGGTTTTTGATGtttt                                                               >  8:1175171/1‑149 (MQ=255)
                                                                                        aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                              <  8:1381568/151‑1 (MQ=255)
                                                                                         gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAAt                                                             <  5:1211609/151‑1 (MQ=255)
                                                                                         gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAAt                                                             <  8:1227620/151‑1 (MQ=255)
                                                                                           cGGTTCAGGAGGATCTCCTGATCCGGCTATTCCGACAGTTTGCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATCACAGGGGAATTATGATGTTTAATGc                                                           <  3:1299848/151‑1 (MQ=255)
                                                                                           cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGc                                                           <  2:863793/151‑1 (MQ=255)
                                                                                           cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGc                                                           <  1:1400093/151‑1 (MQ=255)
                                                                                              ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCGCGTCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGGATTAGGCTATGGGAAGGTGATGAGGTTTTGATCACAGGGGGAGTTTGATGTTAAATGCATg                                                        >  8:543587/1‑151 (MQ=255)
                                                                                                cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGGTATGGCTAGGTGATCAGATTTTGATCACAGGGGAATTATTATGTTTAATTCATgtc                                                      >  6:1778662/1‑149 (MQ=255)
                                                                                                  ggaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAc                                                    <  5:531938/151‑1 (MQ=255)
                                                                                                  ggaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATCCTTCAGATGATGAGTGATGCTGCATTAGGCTAGGGCAAGGTGGTCAGATTTTCATCACAGGGGAATTATGAGGTTAAATTCATGGCAc                                                    >  8:1073585/1‑151 (MQ=255)
                                                                                                   gaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGGATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAcc                                                   <  4:330500/151‑1 (MQ=255)
                                                                                                     ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGCCCGGCCGCCGCCCCCAAATATCCGACAGAGGAGGAGTGAGCCGGCAGGAGGCGAAGGCAAGGTGCTCAGAGTTTGATCACAGGGGAAGTATGATGTTTAATGCATGGCCCCTg                                                 >  4:109048/1‑151 (MQ=255)
                                                                                                      gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGc                                                <  6:582736/151‑1 (MQ=255)
                                                                                                       aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAATGTGATCAGATTTTCATGACAGGGGAATTATGATGTTAAATGCATGGCACCTGcc                                               <  2:1046020/151‑1 (MQ=255)
                                                                                                          tCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCGCCGCCCCCAAATATCCATCAGAGGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAGCAGATTTTCATAACAGGGGAAGTATGATGTTATATTAATGGCACCTGACGGt                                            >  2:1518436/1‑151 (MQ=255)
                                                                                                           ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGt                                            <  8:730737/150‑1 (MQ=255)
                                                                                                           ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTg                                           <  8:332595/151‑1 (MQ=255)
                                                                                                               aTCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAAAATCCTTCAGATGATGAGTGATCATGCATTAGGCTATGGCAAGGGGATAAGATTTTAATCAAACGGGATTTATGATGTTAAATCAATTGCACCTGCCGGTGcccc                                       >  7:1440270/1‑151 (MQ=255)
                                                                                                                tCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTGCGCCCCCCACAATCCTTCAGATGTTGAGTGATCCTGCATTAGGGTATTGCAAGGTGAGCAGATTTTGATCTCAGGTGTATTATGATTTTAAATGCATTGCACCTGCAGGTGccccc                                      >  1:867235/1‑151 (MQ=255)
                                                                                                                 ccGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGCAATTATGATGTTAAATGCATGGCACCTGCCGGTGccccc                                      <  2:606722/150‑1 (MQ=255)
                                                                                                                   ggCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGCTCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGCATTATGATTTTAAATGAATGGCACCTGCCGGTGCCCCCAtt                                   >  6:448025/1‑151 (MQ=255)
                                                                                                                    gCTATTCCGAAAGTTATCCGCCGCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGGATTTGTCTATGGGAAGGTGATCAGATTGTGATCTCAGGGGCATTATGATTTTAAATTCATTGAACCTGACGGTGCCCCCAttt                                  >  1:1794239/1‑151 (MQ=255)
                                                                                                                       aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                               <  3:28224/151‑1 (MQ=255)
                                                                                                                       aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGt                                <  7:1662587/150‑1 (MQ=255)
                                                                                                                         tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                              <  1:1574574/150‑1 (MQ=255)
                                                                                                                         tCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCAGTGAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATGAGATTTTAATCACAGGGGAATTATGATGTTAAATTAATGGCACCTGCCGGGGCCCCCATTTGTTa                              >  4:1487831/1‑150 (MQ=255)
                                                                                                                          ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaa                             <  5:1648469/150‑1 (MQ=255)
                                                                                                                           cGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                           <  7:1519642/151‑1 (MQ=255)
                                                                                                                             aacggtATCCGCCCCCGGCCTGCTCCCCCAAATATCGTTCAGATGATGAGTGATCCTGCATTAGGCTATGGGAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaa                         <  5:842293/146‑1 (MQ=255)
                                                                                                                                 ttATCCGCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAaaaagc                      >  1:814590/1‑150 (MQ=255)
                                                                                                                                  tATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagca                     <  1:24800/150‑1 (MQ=255)
                                                                                                                                  tATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagca                     <  8:1791082/150‑1 (MQ=255)
                                                                                                                                   aTCCGCCCCCGGCCTCCGCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGGAAGGTGATAAGATTTTCATAACAGGGGAATTATGATTTTAAATGCATGGCACCTGCCGGTGGCCCCATTTTTTAAAaaaagcaa                    >  3:652591/1‑150 (MQ=255)
                                                                                                                                    tCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCAGCATTAGGCTATGGCAAGGTGATCAGATTTTCATAACACGGGAATTTTGATGTTAAATTCATGGCACCGGCCGGTGCGCCCCTTTTGTAAAaaaagcaaa                   >  8:1565768/1‑150 (MQ=255)
                                                                                                                                     ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa                 <  4:1207879/151‑1 (MQ=255)
                                                                                                                                      cGCCCCCGTCCTCCTCCCCCAAATATACTTAAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa                    >  8:630371/1‑147 (MQ=255)
                                                                                                                                         ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa             <  4:820945/151‑1 (MQ=255)
                                                                                                                                          cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc            <  5:625971/151‑1 (MQ=255)
                                                                                                                                           ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt           <  3:784285/151‑1 (MQ=255)
                                                                                                                                               cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCt        <  7:1241811/150‑1 (MQ=255)
                                                                                                                                                ctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc       >  7:446538/1‑150 (MQ=255)
                                                                                                                                                ctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc       >  5:1279432/1‑150 (MQ=255)
                                                                                                                                                 ccctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAt     <  4:472817/150‑1 (MQ=255)
                                                                                                                                                  cctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAtt    <  4:127537/151‑1 (MQ=255)
                                                                                                                                                  cctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAt     <  4:933617/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
GTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTAC  >  NC_000913/422287‑422585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑