BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,145	0	T	C	22.4%	382.5 / 122.6	243	intergenic (‑10/+53)	argZ/argY	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (66/97); new base C (10/37); total (105/138)
Fisher's exact test for biased strand distribution p-value = 1.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.14e-02

Only 100 of 263 total aligned reads displayed.

AGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACT  >  NC_000913/2817997‑2818294

 gTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAactt                                                                                                                                                        >  7:40540/1‑150 (MQ=38)

    ccTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAaattaatt                                                                                                                                                    >  8:786639/1‑151 (MQ=255)

     cTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGACCAGTTCACAAAAAAGCTCAGATACGGATTCAAACGGAAAAATAtttt                                                                                                                                                   >  7:883797/1‑151 (MQ=14)

      ggCGGATAATTCCGCTAGCTCTTCGATACCTTCATTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTa                                                                                                                                                  <  1:336415/150‑1 (MQ=255)

       taggTAAATTACGCTACCTCTTCTATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTa                                                                                                                                                  <  2:1080379/148‑1 (MQ=14)

       gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGACatctctatagc                                                                                                                                                 >  7:643659/1‑142 (MQ=255)

       gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTa                                                                                                                                                  <  1:591745/150‑1 (MQ=255)

       gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTa                                                                                                                                                  <  3:183560/150‑1 (MQ=255)

       gCGGTTAATTACTCGACCTCTTCCAAACCTTCATTCCTGCATATGTAGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTa                                                                                                                                                  <  6:1680014/150‑1 (MQ=14)

        cGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCAAACCAACAGCGCAAAGGATGCAAAGAGAAAATTATTTTaat                                                                                                                                                >  2:1434343/1‑149 (MQ=14)

        attttAATTCCTCTAGCTATTCTAAACCTTCATTGCTGAAGATTTAGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttactttact                                                                                                                                                <  4:100607/148‑1 (MQ=14)

         ggTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGCGCTACGGATGCATCGGGAAATTTATTTTActg                                                                                                                                               >  5:730230/1‑151 (MQ=255)

          ttaaaTTCCGGCACCTCTTCCAAACGATCATTCAGGAAGATTTAGTATTAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTActg                                                                                                                                               <  8:376720/147‑1 (MQ=11)

          gTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActg                                                                                                                                               <  1:1474648/150‑1 (MQ=9)

           ttAATTCCGCTACCTCTTCCAAACCTTCATAGCTGCAGATCTCGTACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTActgc                                                                                                                                              <  7:1293460/150‑1 (MQ=14)

            tAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                                                                                                                             <  6:1017233/150‑1 (MQ=255)

            tAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                                                                                                                             <  8:1374305/150‑1 (MQ=255)

             aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGACCCTCCGCCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCCTCGGGAAATTTACTTTActgc                                                                                                                                              >  5:664513/1‑148 (MQ=255)

             aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgctg                                                                                                                                            >  5:826550/1‑150 (MQ=255)

             aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGAGACGCGACTACCACCCGTCTGTACTGAGCGCAGTACTCTGAAGAGCTGAGGTACGGATGCATCGGGAAAATTAATTTActgctgg                                                                                                                                           >  7:709572/1‑150 (MQ=9)

              aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAGTTTACTGCTGa‑‑t                                                                                                                                        >  6:1361439/1‑151 (MQ=255)

              aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTActgc                                                                                                                                              >  5:1626405/1‑147 (MQ=255)

               ttcctgtagatctTCGTTACCTTAATTGCTGAATATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGa‑‑t                                                                                                                                        <  6:1456172/140‑1 (MQ=11)

                 ccGCTACCTCTTCTATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TAtt                                                                                                                                     <  2:433191/151‑1 (MQ=255)

                  cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTg                                                                                                                                    <  3:921968/151‑1 (MQ=255)

                  cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTATATCCAAATCACCTACTGATGCATAAGGAAATTTATTTTAATGGTGA‑‑TAttt                                                                                                                                    >  2:1010545/1‑150 (MQ=14)

                      atctctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa‑‑t                                                                                                                                        >  7:1403049/3‑143 (MQ=9)

                      aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGTTTCATCCGTGAGGATTCGAACCTCCGACCTCTCTGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGTAAATTTATTTTACTGCTGA‑‑TATTGAt                                                                                                                                  <  2:402021/149‑1 (MQ=14)

                      aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGTATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATAc                                                                                                                                <  5:1561192/151‑1 (MQ=255)

                      aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATa                                                                                                                                 <  4:1706081/150‑1 (MQ=255)

                      aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTAGAACATCAGACAGCTCGGTTCGTAGAAGAGTACTATATCAATATGAGATACTGATGGATCGGTAATTTTATTTTACTGCTGA‑‑TATTGATa                                                                                                                                 >  2:696553/1‑150 (MQ=14)

                      aCCTCTCCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAGTTTACTGCAGA‑‑TAATgtgcg                                                                                                                                >  1:1828123/1‑147 (MQ=14)

                          ctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                                                                                                                             <  5:1474088/136‑1 (MQ=18)

                          ctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                                                                                                                             >  2:1475780/1‑136 (MQ=18)

                          ctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATATATTTTAATGCAGa‑‑gataggaagagcc                                                                                                                            >  4:32627/1‑141 (MQ=11)

                           ttCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCAGA‑‑TAtttttatagct                                                                                                                            >  7:834887/1‑141 (MQ=14)

                            tCGATCCGTTCATAGCTGAAGATTTCGGAGCATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGA‑‑ttttttgatacc                                                                                                                             <  6:394191/148‑9 (MQ=11)

                            tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGct                                                                                                                            <  5:614780/149‑1 (MQ=255)

                             cGATACCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                                                                                                                             <  8:1405060/133‑1 (MQ=25)

                             cGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATATATTTAAATGCTTA‑‑TATTTATAACGcaaa                                                                                                                          >  3:1515182/1‑147 (MQ=11)

                             cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGctact                                                                                                                         <  4:1213929/151‑1 (MQ=255)

                             cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGAGATCGGTACAGCTCAGCTACGGAGGCAAATCGAAAGTTAGTTTACTGCTGA‑‑TATTGATACCGctact                                                                                                                         >  1:1735746/1‑151 (MQ=14)

                              gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGctacta                                                                                                                        <  3:40298/151‑1 (MQ=255)

                              gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTATTTTACTGCTGA‑‑TAtttagagacctcgt                                                                                                                         >  1:405109/1‑138 (MQ=255)

                              gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTAATTTACTGCAGA‑‑TATTtataaacctaca                                                                                                                         >  2:749190/1‑142 (MQ=11)

                                  ccTTGATTTATGAATATTTCTTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAg                                                                                                                     <  3:776776/150‑1 (MQ=14)

                                    gtaaTTGATGAATATTTCGTACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGc                                                                                                                    <  5:1221328/148‑1 (MQ=14)

                                     tCATTGCTGAAGTTTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCa                                                                                                                  <  5:1355724/150‑1 (MQ=255)

                                     tCATTGATGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAg                                                                                                                     <  8:1038336/149‑1 (MQ=11)

                                      cATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActg                                                                                                                                               >  6:507848/1‑122 (MQ=18)

                                      cATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActg                                                                                                                                               <  4:510669/122‑1 (MQ=18)

                                      cATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAATTTACTGCTGA‑‑TAtt                                                                                                                                     >  8:884151/1‑130 (MQ=255)

                                      aatatcTGAATATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGccg                                                                                                                  <  2:1263410/146‑2 (MQ=11)

                                      aaTTGCTTAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCata                                                                                                                <  3:1099839/150‑1 (MQ=255)

                                         gccTGAAGATCTCTGCGCATATGGTGCATCCGGGAGGATTCGAACCTAGGTTTTATTTGTTCCTAGGCGTGTACTCTATCGATCTTATCTAGGGATGCATGTTTAAATTTATTTTACTGTTGA‑‑TATTGATACCGCTACTAAAGCCATATCa                                                                                                             <  2:893987/149‑1 (MQ=9)

                                            tGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgc                                                                                                                                              >  1:91937/1‑117 (MQ=21)

                                            gcaaGATTTCGTACTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCaa                                                                                                            <  6:577032/149‑1 (MQ=14)

                                               aGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAag                                                                                                       <  6:1224731/151‑1 (MQ=255)

                                                    tCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTTATACAGCTACTAAAGCCAGGTCAAGTAAGAGATg                                                                                                  >  5:388894/1‑151 (MQ=255)

                                                      ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAc                                                                                                                                                 >  7:311318/1‑104 (MQ=40)

                                                       gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTg                                                                                               <  2:730611/151‑1 (MQ=39)

                                                       gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGAGGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATAGCGCTACTAAAGCCAGATCAAGTATGAGATGGTg                                                                                               >  5:1039567/1‑151 (MQ=255)

                                                        agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAATTAACATCTTA‑‑TTTTTCAACCCCAAATAAAGCCATATAAAATAAGAAATGGt                                                                                                >  4:1426402/1‑149 (MQ=11)

                                                         gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAattt                                                                                                                                                        <  4:691262/94‑1 (MQ=32)

                                                           atatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATGTAtttt                                                                                                                                                   >  5:168115/1‑97 (MQ=25)

                                                             atGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATcc                                                                                          <  1:1006488/150‑1 (MQ=40)

                                                             atGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATcc                                                                                          <  8:613649/150‑1 (MQ=40)

                                                               gggccATCCGGCAGTATTCGAACCTCCGACCGCTCGGTGCGTAGCCGAGTACTTAATCCCCCTGAGATACGGTTGCATGGGTAAACTTATTATACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCggg                                                                                       <  1:82759/147‑1 (MQ=11)

                                                                 tGCATCCGAGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAGTTTACTGCTGA‑‑TATTTAGAGAGCTACTAAAGACAGATCAAGTATGAGATGGTGCATcagcg                                                                                       >  6:1124191/1‑145 (MQ=14)

                                                                    aTCCGGGAGGATTACAACCTAAGACCGAACGCTTAGTACCCGAGAACTCAATCAAGCTGAGCTAAGGATGCATCTGGAAATTTATTTTACTGCTGA‑‑TATTGATAAAAATAAAAAAGCCATATCAAGTAAGAGATGGTGCGTCCGGGAGGAt                                                                                  >  4:431660/1‑151 (MQ=255)

                                                                     tCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCAGA‑‑TATAGGTACCGCTACTAAAACGGTATCAATTTAGCGATTATGGTTCAGGGAGGAtt                                                                                 >  2:604074/1‑151 (MQ=11)

                                                                      ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGC‑‑TATTg                                                                                                                                    >  6:1590518/1‑99 (MQ=255)

                                                                      ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTg                                                                                                                                    <  4:1598781/99‑1 (MQ=255)

                                                                      ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTTATAGAGCTAGTAAAGCCAAATCAAGTAAGAGATGGTGCATCCGGGAGGAtt                                                                                 >  2:1769928/1‑150 (MQ=255)

                                                                              aTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGacgcgcc                                                                        >  6:1789137/1‑145 (MQ=255)

                                                                                tCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGacgcgccgc                                                                      >  5:1110965/1‑143 (MQ=255)

                                                                                tCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGAATCAGGGAGGAttatacgcgccg                                                                       >  2:334931/1‑140 (MQ=14)

                                                                                  gAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAtttt                                                                                                                                                   <  7:445316/74‑1 (MQ=38)

                                                                                  gAACCTCCGACCGCGCGGTTCGTAGCCGAGTACTCTATCCATCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTCAAACCGCTACTACAGCAATATCACGTACCATATGGTGCATCCGGGAGGATTCGAACCTCCGAcc                                                                    <  1:247545/151‑1 (MQ=17)

                                                                                   aaCCTACGAGCGCTCGGTTCGTAGCCGAGTAGTCTATCCCGATGCTCTACTTACGCATCGGGAAATTTAATTTACTGCTGA‑‑TATTGATAACGCTACTAAATCAATATCAAGTAACATATGGTGCATCCGGGAGGATTCGAACCTCCGACCg                                                                   <  6:424133/151‑1 (MQ=17)

                                                                                     ccgcagcgctcggggTTCGTCGCCGAGTACTCTACCCACCTCAGCTACGGATGCATCGGCAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                                                                 <  5:1420944/138‑1 (MQ=17)

                                                                                      cTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatttat                                                                                                                                                      >  4:109439/1‑67 (MQ=35)

                                                                                      cTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATAACCCTAGTAAAGCCAAATCAAGAAAGAGATGGTGAATCAGGGAGGATTAGactcgacgagcgaa                                                                 >  3:252038/1‑137 (MQ=14)

                                                                                           cccgcgccgctCGTAGCCGAGTACTCTAACCCCCTCCGCTACCGCTGCATCGGGTCATTTATTTTACTGCAGA‑‑TCTTGTTTCCGCTAATATAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTc                                                          <  5:962063/141‑1 (MQ=18)

                                                                                             cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTTCTGa‑‑ta                                                                                                                                       >  7:520647/1‑73 (MQ=255)

                                                                                              gCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACGCTCCGAGCggagcgttcgtt                                                        >  5:215373/1‑140 (MQ=14)

                                                                                              gCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAATGCTGA‑‑TTTTGATa                                                                                                                                 >  7:528647/1‑78 (MQ=255)

                                                                                                 gggcgcggaGCCGAGTACTCTATCCCGCAGAGCTACGTCTGCAGCGGGAAATTTATTTTACTGCAGA‑‑TATTCCAACCGCTAATACATCCATATCAAGTAACATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGcc                                                    <  5:448576/143‑1 (MQ=18)

                                                                                                    gtCGTAGCCGGGTACGCTTTCCCCCTGCGCTACGGATGCAGCGGGAAAGTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAg                                                 <  6:473201/150‑1 (MQ=18)

                                                                                                         aGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATCGTGAATCCGGGAGGATAAGAAACTCCTAGCGAAAC‑GAACGTAGCCGAGTaat                                             >  8:1832690/1‑148 (MQ=14)

                                                                                                         aGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTAATAAACACATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACGCGCAGAGC‑CTCGCGCTCGTAGCCGAGTaga                                             >  6:837615/1‑148 (MQ=14)

                                                                                                            cgcgTACTCTATCCAGCCGCGCTACCGCGCCAGCGGGAACGTTCTTTTACTGGTGA‑‑TATTCCAACCGCTCCTAACGGAAGATCAAGTACGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTAt                                         <  6:311436/148‑1 (MQ=12)

                                                                                                                tACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGAATAAGCGAGGATTAGACGCGCCGAGCGGAGC‑GATAGTAGCCGAGTagaaaaacca                                      >  8:829769/1‑141 (MQ=14)

                                                                                                                   tctATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAACCTCCGACCCAACC‑CTTCGTAGCCGATTagaaagcaaacgat                                  >  8:435116/1‑138 (MQ=17)

                                                                                                                                gCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGc                      >  7:1237870/1‑150 (MQ=255)

                                                                                                                                              ggaaaaCTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCg                                                                       >  7:1138230/4‑88 (MQ=11)

                                                                                                                                              ggaaaaCTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCg                                                                       <  8:1143161/85‑1 (MQ=255)

                                                                                                                                               tgAAATTTATTTTACTGCTTA‑‑TATTGATACCGCTACTAAAGCGATATCAAGTAATAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCaaatggcggtgaggc       <  4:1160141/149‑15 (MQ=17)

                                                                                                                                               ggAAATTTTATTTACGGAAGA‑‑TATTCCAACCGCTCATAAAGCCATATTTAGTACCATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttactt      <  5:1752784/151‑1 (MQ=14)

                                                                                                                                                   atttatttTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGCCCCGAGC‑GTTAGTGGCCGAGTATTAAATCCAGCTAAGCTCCCGATGCAGCGCGGAacttacgtgacc  >  7:1806275/1‑145 (MQ=17)

AGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACT  >  NC_000913/2817997‑2818294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`