Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,188,572 C→A R1075S (CGT→AGT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,188,5720CA99.0% 1849.9 / 4.7 493R1075S (CGT→AGT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (4/1);  new base A (232/256);  total (236/257)
Fisher's exact test for biased strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 493 total aligned reads displayed.

GCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGT  >  NC_000913/4188424‑4188718
                                                                                                                                                    |                                                                                                                                                  
 ccGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTc                                                                                                                                                 <  2:698952/151‑1 (MQ=255)
  cGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTcc                                                                                                                                                >  4:276536/1‑151 (MQ=255)
  cGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCACTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTcc                                                                                                                                                >  2:317339/1‑151 (MQ=255)
   ggTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCg                                                                                                                                               <  6:1446079/151‑1 (MQ=255)
       aTCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCa                                                                                                                                            <  2:822496/150‑1 (MQ=255)
       aTCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCAc                                                                                                                                           >  5:350565/1‑151 (MQ=255)
         cACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTg                                                                                                                                         >  1:829035/1‑151 (MQ=255)
         cACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTg                                                                                                                                         >  8:1789252/1‑151 (MQ=255)
             gAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGaaaa                                                                                                                                     <  8:510863/151‑1 (MQ=255)
               aGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATc                                                                                                                                   <  1:1187001/151‑1 (MQ=255)
               aGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATc                                                                                                                                   >  4:1620539/1‑151 (MQ=255)
                  aaGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGtt                                                                                                                                <  1:1723058/151‑1 (MQ=255)
                  aaGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGtt                                                                                                                                <  3:933221/151‑1 (MQ=255)
                   aGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTg                                                                                                                               >  3:754448/1‑151 (MQ=255)
                     cGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCTCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCCGAGTCCGGCACTGAAAATCGTTGAt                                                                                                                             <  5:147156/151‑1 (MQ=255)
                     cGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGAt                                                                                                                             <  7:110177/151‑1 (MQ=255)
                     cGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGAt                                                                                                                             <  1:1595253/151‑1 (MQ=255)
                      ggTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATg                                                                                                                            >  1:239878/1‑151 (MQ=255)
                      ggTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATg                                                                                                                            <  2:992588/151‑1 (MQ=255)
                          ttGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTc                                                                                                                         >  5:933318/1‑150 (MQ=255)
                            gTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAgg                                                                                                                      <  3:676694/151‑1 (MQ=255)
                            gTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAg                                                                                                                       <  4:1752937/150‑1 (MQ=255)
                            gTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAg                                                                                                                       <  3:990125/150‑1 (MQ=255)
                              aggCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGt                                                                                                                    <  1:929405/149‑1 (MQ=255)
                              aCGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAggg                                                                                                                     <  3:517375/150‑1 (MQ=255)
                               cGCTTTACGGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTTTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTa                                                                                                                   <  1:590504/151‑1 (MQ=255)
                                gCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTa                                                                                                                   >  1:1782675/1‑150 (MQ=255)
                                  tttACTGACATGATCGACGGCCAGACCATTACGCGTCAGCCCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                                >  4:943624/1‑151 (MQ=255)
                                     aCTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacgacg                                                                                                             >  3:297445/1‑151 (MQ=255)
                                          cATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTg                                                                                                        <  2:244953/151‑1 (MQ=255)
                                          cATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTg                                                                                                        >  3:1740953/1‑151 (MQ=255)
                                           aTGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGCTCGTAAAGCTCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTACCGACCGTCTg                                                                                                        >  4:385156/1‑150 (MQ=255)
                                             gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATc                                                                                                     <  3:1318715/151‑1 (MQ=255)
                                             gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATc                                                                                                     <  6:1298489/151‑1 (MQ=255)
                                              aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATcc                                                                                                    >  5:729595/1‑151 (MQ=255)
                                              aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATcc                                                                                                    >  2:152228/1‑151 (MQ=255)
                                              aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATcc                                                                                                    >  6:1722578/1‑151 (MQ=255)
                                                cGACGGCCAGACCATTACGCGTCAGACCGACGAACTGAGCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCa                                                                                                  <  3:387975/151‑1 (MQ=255)
                                                cGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATcccc                                                                                                  >  2:1578095/1‑150 (MQ=255)
                                                  aCGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAgg                                                                                                <  5:806336/151‑1 (MQ=255)
                                                   cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                               <  5:1016770/151‑1 (MQ=255)
                                                   cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                               >  8:267986/1‑151 (MQ=255)
                                                      ccAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTAcc                                                                                            >  5:1544588/1‑151 (MQ=255)
                                                       cAGACCATTACGCGTCAGACCGAAGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCg                                                                                           <  7:1335867/151‑1 (MQ=255)
                                                        aGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCg                                                                                           >  4:1781372/1‑150 (MQ=255)
                                                         gACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGat                                                                                         <  6:965034/151‑1 (MQ=255)
                                                            cATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATg                                                                                      <  2:108751/151‑1 (MQ=255)
                                                            cATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATg                                                                                      <  5:476558/151‑1 (MQ=255)
                                                             aTTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                                     >  7:1146697/1‑151 (MQ=255)
                                                                aCGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCAGTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCa                                                                                   <  6:796276/150‑1 (MQ=255)
                                                                    gTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcgc                                                                               >  8:1175276/1‑150 (MQ=255)
                                                                    gTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCa                                                                              <  4:626211/151‑1 (MQ=255)
                                                                     tCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcgc                                                                               >  4:878722/1‑149 (MQ=255)
                                                                      cAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAg                                                                             <  5:985246/150‑1 (MQ=255)
                                                                         aCCGACGAACTGACCGGTCTGTCTTCGCTGGGGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACt                                                                         <  1:1296562/151‑1 (MQ=255)
                                                                           cgacgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTc                                                                       >  7:864851/1‑151 (MQ=255)
                                                                            gacgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTcc                                                                      <  3:1803553/151‑1 (MQ=255)
                                                                              cgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTg                                                                    <  6:1584910/151‑1 (MQ=255)
                                                                                aaCTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGcc                                                                  <  7:163432/151‑1 (MQ=255)
                                                                                aaCTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGcc                                                                  >  3:870047/1‑151 (MQ=255)
                                                                                 aCTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCg                                                                 >  3:1487080/1‑151 (MQ=255)
                                                                                    gACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGt                                                              >  5:637291/1‑151 (MQ=255)
                                                                                       cGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTaa                                                            >  4:490472/1‑150 (MQ=255)
                                                                                        ggTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTaa                                                            >  1:351105/1‑149 (MQ=255)
                                                                                          tctgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCg                                                        >  7:524900/1‑151 (MQ=255)
                                                                                              tctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTg                                                    >  6:619517/1‑151 (MQ=255)
                                                                                                  cGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTc                                                 <  7:251190/150‑1 (MQ=255)
                                                                                                   gCTGGTGTTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAACTCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAg                                               <  5:1840867/151‑1 (MQ=255)
                                                                                                   gCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTc                                                 >  2:1237953/1‑149 (MQ=255)
                                                                                                   gCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAg                                               <  6:721662/151‑1 (MQ=255)
                                                                                                      ggtggtTCTGGATTCCGCAGTACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCt                                             <  8:1261456/150‑1 (MQ=255)
                                                                                                       gtggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTgg                                           <  8:835893/151‑1 (MQ=255)
                                                                                                            tCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                          >  2:14397/1‑147 (MQ=255)
                                                                                                               ggATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGc                                   <  2:1752937/151‑1 (MQ=255)
                                                                                                               ggATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGc                                   >  8:651269/1‑151 (MQ=255)
                                                                                                               ggATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGc                                   >  5:114288/1‑151 (MQ=255)
                                                                                                               ggATGCCGCAGAACGTACCGCAGGTGGTAAAGAGCTGAGTCCGGCACTGAAACTCGTTGATGCTCAGGGTAACGACGGTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATg                                     <  4:1205821/149‑1 (MQ=255)
                                                                                                                    ccGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTAc                               <  8:432987/150‑1 (MQ=255)
                                                                                                                       cAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGAt                           <  4:1485641/151‑1 (MQ=255)
                                                                                                                          aaCGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTGCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAg                        <  6:482386/151‑1 (MQ=255)
                                                                                                                           aCGTACCGCAGGTGTTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGc                       <  7:630916/151‑1 (MQ=255)
                                                                                                                            cGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGct                      >  5:1293745/1‑151 (MQ=255)
                                                                                                                             gTACCGCAGTGGGTAAAGATCTGATTCCGGCACTGTAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATCCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGctc                     <  8:1622383/151‑1 (MQ=255)
                                                                                                                             gTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGctc                     >  4:1538268/1‑151 (MQ=255)
                                                                                                                             gTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGctc                     <  1:458905/151‑1 (MQ=255)
                                                                                                                               aCCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGctct                    <  4:721367/150‑1 (MQ=255)
                                                                                                                               aCCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTg                   <  5:265994/151‑1 (MQ=255)
                                                                                                                                ccGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGTAGATGGCGTACAGATCAGCTCTgg                  <  6:276892/151‑1 (MQ=255)
                                                                                                                                 cGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGt                 <  2:1356479/151‑1 (MQ=255)
                                                                                                                                 cGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGt                 >  1:1034098/1‑151 (MQ=255)
                                                                                                                                 cGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGt                 <  5:1361563/151‑1 (MQ=255)
                                                                                                                                  gCATGTGGTAAATATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGAAGTTCTGCTCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTg                <  6:705089/151‑1 (MQ=255)
                                                                                                                                    aGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGac              >  3:1081376/1‑151 (MQ=255)
                                                                                                                                       tggtAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCGTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACAcc           >  8:1570451/1‑151 (MQ=255)
                                                                                                                                        ggtAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACAccc          <  7:422357/151‑1 (MQ=255)
                                                                                                                                        ggtAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGAAATGTAAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGCTCACAccc          >  6:560249/1‑151 (MQ=255)
                                                                                                                                             aGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGgcg     <  8:721299/151‑1 (MQ=255)
                                                                                                                                               aTCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGcgcg   >  1:1434404/1‑151 (MQ=255)
                                                                                                                                                tCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGt  >  7:1507229/1‑151 (MQ=255)
                                                                                                                                                 cTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGt  >  7:334640/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGT  >  NC_000913/4188424‑4188718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: