Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,368,214 C→T Q130* (CAG→TAG)  yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,2140CT99.8% 1898.3 / NA 508Q130* (CAG→TAG) yhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (276/230);  minor base A (1/0);  total (277/231)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 508 total aligned reads displayed.

GGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCG  >  NC_000913/3368067‑3368360
                                                                                                                                                   |                                                                                                                                                  
 ggTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGAc                                                                                                                                                >  2:1775795/1‑151 (MQ=255)
 ggTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGAc                                                                                                                                                <  3:1091830/151‑1 (MQ=255)
    tctcTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGc                                                                                                                                              <  4:342311/150‑1 (MQ=255)
    tctcTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCt                                                                                                                                             >  5:1352992/1‑151 (MQ=255)
    tctcTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATCAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGc                                                                                                                                              <  5:20296/150‑1 (MQ=255)
       ctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCt                                                                                                                                             <  2:1307657/148‑1 (MQ=255)
       ctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCt                                                                                                                                             <  7:217874/148‑1 (MQ=255)
       ctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCt                                                                                                                                             <  5:1148920/148‑1 (MQ=255)
         gCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTg                                                                                                                                        >  8:1261820/1‑151 (MQ=255)
            aaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCa                                                                                                                                     <  1:1856364/151‑1 (MQ=255)
             aaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACCCCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCa                                                                                                                                     >  7:1483899/1‑150 (MQ=255)
             aaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCa                                                                                                                                     >  1:569179/1‑150 (MQ=255)
             aaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGCGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCa                                                                                                                                     >  3:1472669/1‑150 (MQ=255)
                aCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATc                                                                                                                                 >  4:1586113/1‑151 (MQ=255)
                 cTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCt                                                                                                                                >  3:987241/1‑151 (MQ=255)
                     cGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGCCACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCGAAATCTTTcc                                                                                                                            >  8:801701/1‑151 (MQ=255)
                       ccAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTcccc                                                                                                                          >  7:1234861/1‑151 (MQ=255)
                        cAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCt                                                                                                                         >  2:1482572/1‑151 (MQ=255)
                         aaaTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCtt                                                                                                                        >  1:277246/1‑151 (MQ=255)
                          aaTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTg                                                                                                                       <  5:108631/151‑1 (MQ=255)
                           aTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGc                                                                                                                      <  2:1055893/151‑1 (MQ=255)
                               ggCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGccc                                                                                                                    >  1:668808/1‑149 (MQ=255)
                                gCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAgg                                                                                                                 >  4:1751283/1‑151 (MQ=255)
                                  ttttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTc                                                                                                               >  2:1672538/1‑151 (MQ=255)
                                     tttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTACGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTacac                                                                                                            >  5:1607633/1‑151 (MQ=255)
                                      ttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAcc                                                                                                           >  3:443315/1‑151 (MQ=255)
                                      ttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAc                                                                                                            <  5:391280/150‑1 (MQ=255)
                                          ctctTCTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                                                       >  2:215252/1‑151 (MQ=255)
                                           tctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGt                                                                                                      >  3:794251/1‑151 (MQ=255)
                                             ttttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTcc                                                                                                    >  6:714074/1‑151 (MQ=255)
                                              tttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGt                                                                                                      <  8:738488/148‑1 (MQ=255)
                                              tttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTcc                                                                                                    <  7:513475/150‑1 (MQ=255)
                                              tttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCg                                                                                                   >  3:143940/1‑151 (MQ=255)
                                              tttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTACCCTTGCCCAGGTCCACCTACGTCCg                                                                                                   >  5:320899/1‑151 (MQ=255)
                                               ttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCg                                                                                                   <  3:677759/150‑1 (MQ=255)
                                               ttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGt                                                                                                  >  2:416982/1‑151 (MQ=255)
                                                   cAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTa                                                                                               <  7:690285/150‑1 (MQ=255)
                                                    aaCATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACt                                                                                             <  6:494919/151‑1 (MQ=255)
                                                      cATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACttt                                                                                           >  7:1107774/1‑151 (MQ=255)
                                                       aTGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTc                                                                                          >  4:570650/1‑151 (MQ=255)
                                                         gTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTcc                                                                                         <  7:333847/150‑1 (MQ=255)
                                                         gTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCg                                                                                        <  4:146719/151‑1 (MQ=255)
                                                           tGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGtt                                                                                      >  4:1719740/1‑151 (MQ=255)
                                                              aaTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaa                                                                                   <  3:364122/151‑1 (MQ=255)
                                                                   aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGc                                                                              <  6:1149145/151‑1 (MQ=255)
                                                                    ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCt                                                                             >  7:1746811/1‑151 (MQ=255)
                                                                     cAGACAATTTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTg                                                                            <  7:186471/151‑1 (MQ=255)
                                                                     cAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTg                                                                            <  7:613596/151‑1 (MQ=255)
                                                                     cAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTg                                                                            <  3:1724002/151‑1 (MQ=255)
                                                                      aGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTg                                                                            >  5:980199/1‑150 (MQ=255)
                                                                      aGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTg                                                                            >  6:374076/1‑150 (MQ=255)
                                                                      aGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGa                                                                           <  5:893654/151‑1 (MQ=255)
                                                                       gACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGAc                                                                          >  7:1016737/1‑151 (MQ=255)
                                                                          aaTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCCCCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGcc                                                                       >  2:525376/1‑151 (MQ=255)
                                                                          aaTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGcc                                                                       >  7:976448/1‑151 (MQ=255)
                                                                           aTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTGAAAACGCTGACGccc                                                                      <  6:895758/151‑1 (MQ=255)
                                                                             gTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGc                                                                    >  8:1077846/1‑151 (MQ=255)
                                                                             gTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGc                                                                    >  3:1305134/1‑151 (MQ=255)
                                                                               cTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGc                                                                    >  5:1218034/1‑149 (MQ=255)
                                                                                 gtcGAATTAACACCATTGGTAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTCCAAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAg                                                                  <  6:1660239/147‑1 (MQ=255)
                                                                                   cGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCttt                                                              <  3:569617/151‑1 (MQ=255)
                                                                                    gAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCACATCGGCAAAGATTTGGAAGACGCTGTCTGCCAAATCTTTCCCCTTCACCAGCTCCACCTACGTCCGTTTACTTTCCGTTAAAAAAGCTGACGCCCGCAGCttat                                                             >  4:964077/1‑149 (MQ=255)
                                                                                         aaCACCATTGGGAATTCCGAAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGa                                                         <  8:1467788/150‑1 (MQ=255)
                                                                                           cacCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTAAGTCCGATTACTTTCCCTTAAAAACGCTGACGCCCGCAGCTTTTATGaaa                                                       >  4:1219913/1‑150 (MQ=255)
                                                                                                 tGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAgaga                                                 <  5:1707212/150‑1 (MQ=255)
                                                                                                  gggAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACg                                               <  8:1675546/151‑1 (MQ=255)
                                                                                                    gAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCATTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCt                                             <  8:1759716/151‑1 (MQ=255)
                                                                                                     aaTTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTc                                            >  1:1132728/1‑151 (MQ=255)
                                                                                                      aTTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTcc                                           >  1:1019539/1‑151 (MQ=255)
                                                                                                      aTTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTcc                                           <  8:1408041/151‑1 (MQ=255)
                                                                                                      aTTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTcc                                           <  5:594223/151‑1 (MQ=255)
                                                                                                       ttCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTcc                                           <  6:1806052/150‑1 (MQ=255)
                                                                                                          cgcccacacCGTCTGATGAATTCCCATCGGCAACTATTTGGTAGACGGTGTCTGCCAAATCTTTCCCCTTGCCCCGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtg                                       <  2:652198/146‑1 (MQ=255)
                                                                                                          cGCAGACACCGTCAGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGGTAAAAACGCTGACGCCCGAAGCTTTTATGAAAAAGAGACGCTCCGCtg                                       >  6:25176/1‑151 (MQ=255)
                                                                                                            cAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgtg                                     <  7:451412/151‑1 (MQ=255)
                                                                                                               acacCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCt                                  <  8:653484/151‑1 (MQ=255)
                                                                                                                  ccGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGt                               <  1:903536/151‑1 (MQ=255)
                                                                                                                   cGTCTGCAGACATCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTc                              <  3:1578192/151‑1 (MQ=255)
                                                                                                                   cGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTc                              >  3:1178724/1‑151 (MQ=255)
                                                                                                                    gTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCt                             >  8:262338/1‑151 (MQ=255)
                                                                                                                    gTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCt                             >  7:834245/1‑151 (MQ=255)
                                                                                                                     tCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTg                            >  1:1815213/1‑151 (MQ=255)
                                                                                                                     gcTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGGCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTg                            <  1:1356530/150‑1 (MQ=255)
                                                                                                                       tGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAATTCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGtt                          >  3:213724/1‑151 (MQ=255)
                                                                                                                       tGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGtt                          >  2:819914/1‑151 (MQ=255)
                                                                                                                        gCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGt                           <  2:1149951/149‑1 (MQ=255)
                                                                                                                        gCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTc                         <  2:970785/151‑1 (MQ=255)
                                                                                                                          aGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGc                       <  1:793714/151‑1 (MQ=255)
                                                                                                                           gAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGcc                      >  4:737762/1‑151 (MQ=255)
                                                                                                                            aaTTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCa                     <  6:1720761/151‑1 (MQ=255)
                                                                                                                             aTTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAg                    <  1:1224830/151‑1 (MQ=255)
                                                                                                                              ttCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGc                   >  4:1066007/1‑151 (MQ=255)
                                                                                                                                 ccATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCt                  >  8:457019/1‑149 (MQ=255)
                                                                                                                                     cGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGc             <  7:1399450/150‑1 (MQ=255)
                                                                                                                                     cGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCg            >  1:1720378/1‑151 (MQ=255)
                                                                                                                                       gCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGc          <  4:1336446/151‑1 (MQ=255)
                                                                                                                                           aGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAAt      <  4:501837/151‑1 (MQ=255)
                                                                                                                                            gATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAAtt     <  6:1506825/151‑1 (MQ=255)
                                                                                                                                               ttGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTATATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCg  >  6:670602/1‑151 (MQ=255)
                                                                                                                                                tGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCg  >  1:1481774/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCG  >  NC_000913/3368067‑3368360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑