Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4320TG24.7% 838.1 / 75.8 402intergenic (+73/‑86)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (85/210);  new base G (97/0);  total (191/211)
Fisher's exact test for biased strand distribution p-value = 2.92e-41
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 427 total aligned reads displayed.

TCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTC  >  NC_000913/422283‑422580
                                                                                                                                                     |                                                                                                                                                     
tCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcct                                                                                                                                                       <  8:1155015/150‑1 (MQ=255)
 cgcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcc                                                                                                                                                     <  8:643559/151‑1 (MQ=255)
 cgcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGgccgcc                                                                                                                                                     >  6:201789/1‑151 (MQ=255)
  gcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcct                                                                                                                                                    <  7:648051/151‑1 (MQ=255)
       gATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGCCGGCCTCCTccccc                                                                                                                                               >  1:219789/1‑151 (MQ=255)
        aTTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCGCGGGCTCCgccgccc                                                                                                                                              >  5:68483/1‑150 (MQ=255)
              gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAatat                                                                                                                                         <  3:491010/150‑1 (MQ=255)
               tgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGACCCCGGCGACCGCCAGCAAATATc                                                                                                                                        >  5:1302736/1‑150 (MQ=255)
                gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATcc                                                                                                                                       <  8:416850/150‑1 (MQ=255)
                gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATcc                                                                                                                                       <  5:424512/150‑1 (MQ=255)
                gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCt                                                                                                                                      <  6:524701/151‑1 (MQ=255)
                gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCt                                                                                                                                      >  1:1245420/1‑151 (MQ=255)
                  tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGGCGGCCTCCGCCCCCCAAGATCCtgc                                                                                                                                    >  7:1843243/1‑149 (MQ=255)
                     tGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAg                                                                                                                                  <  4:1086682/150‑1 (MQ=255)
                     tGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAg                                                                                                                                  <  2:15955/150‑1 (MQ=255)
                          ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatgat                                                                                                                             <  4:1698010/150‑1 (MQ=255)
                            cTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCCAATATCCTTAAGatgatg                                                                                                                            >  2:1372325/1‑149 (MQ=255)
                              ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGGCGGCCCCCAATATCGTTGCGATGCtgtgtg                                                                                                                        >  1:1128223/1‑151 (MQ=255)
                              ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAg                                                                                                                          <  8:1444422/149‑1 (MQ=255)
                                    ttAAACGCCGCCACGATCGTCAGCTGGCTCAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCCTACACCACCCCCAAATATACATGAAATGATGAGGGaaca                                                                                                                    >  7:1429123/1‑146 (MQ=255)
                                      aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCCAATAACCTTCAGATGATGAGTGAtgctgc                                                                                                                 >  4:180121/1‑150 (MQ=255)
                                      aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGGtgctgc                                                                                                                 >  4:1445501/1‑150 (MQ=255)
                                       aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCACATATACTTCAGATGATGTGTGGTCCTGCAt                                                                                                               >  2:796709/1‑151 (MQ=255)
                                          gccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCTCCACCGGCCCCAAAAATCCTGCAGATGATGAGTGATCCTGCAtt                                                                                                              >  7:1306566/1‑149 (MQ=255)
                                           gctccACGATCCTCAGCTGGCTGAAAACCATTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                           <  6:148982/148‑1 (MQ=255)
                                             gccACGATCGTCAGCTGGCTGAATACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCt                                                                                                         <  1:1050616/151‑1 (MQ=255)
                                             gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCATGATCCCGATATTCCGAAAGTAATCCGGGCCAGTCCTCCTCCCGCGAATATCCTGAAATTGCACAGTGATGCTGCATTAGGCt                                                                                                         >  8:1734358/1‑151 (MQ=255)
                                               cACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCGGCATTAGGCTAt                                                                                                       >  3:123863/1‑151 (MQ=255)
                                                aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                      <  6:963079/151‑1 (MQ=255)
                                                   aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCGCCCCCAAAAATCCGACAGAAGAGGAATGATCCAGGAATAggggaggga                                                                                                    >  7:834229/1‑143 (MQ=255)
                                                   aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                                   <  2:917454/151‑1 (MQ=255)
                                                   aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                                   <  5:597328/151‑1 (MQ=255)
                                                   aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGGCGCCCCCCAATATCCGGCCGAGGATGAGGGGTGCGGGGTTAGGGTATggg                                                                                                    >  5:1694603/1‑149 (MQ=255)
                                                     cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAg                                                                                                 <  5:88388/151‑1 (MQ=255)
                                                        cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGt                                                                                               <  7:379105/150‑1 (MQ=255)
                                                        cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATAACCGTCACATGATGAGTGATCCTGCAGTAGGCTATGGCCAGGTg                                                                                              >  3:1005774/1‑151 (MQ=255)
                                                        cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAAAATCCTTCAGATGATGAGTGATCCTGCATTAGGCTAAGGCaaaggt                                                                                              >  7:978188/1‑147 (MQ=255)
                                                        cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCCCCCCCGGCCTCCGCCGCCAAATAACGTTAAGTTCTTGAGTGAACGTGCATTAGCGTATgggtatgtg                                                                                              >  6:1135307/1‑144 (MQ=255)
                                                         agatggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCCCCCCCGGCCACCGCCCCCAAATATAAAACAGATGATGAGTCATCCAGCAGTAGTCTaggaaaagggg                                                                                              >  4:238944/4‑140 (MQ=255)
                                                         aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCACCTGCCCCAAATAAACATGAGATGATGAGTGGTCATGCATTAGGGTATGGGAAGCTgt                                                                                             >  4:487133/1‑150 (MQ=255)
                                                          gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCTCCCCCAAATATCCGTCAGAGGATGAGTGATCCTGCATTAGGGGATGGCAAGGTGAt                                                                                            >  2:1306297/1‑151 (MQ=255)
                                                             ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATc                                                                                           <  6:1629314/149‑1 (MQ=255)
                                                              gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGa                                                                                        <  6:1590707/151‑1 (MQ=255)
                                                                tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGGGATGCTGCATGAGGCTATGGGAAGGTGCTCAGCtt                                                                                      >  2:1114423/1‑151 (MQ=255)
                                                                  aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtttt                                                                                    <  5:176624/151‑1 (MQ=255)
                                                                   aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtc                                                                                   <  4:58213/151‑1 (MQ=255)
                                                                   aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTGCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtc                                                                                   <  4:82580/151‑1 (MQ=255)
                                                                    aaCCATTAATCCTTTCGTTAAGTTCTCCGGCTATGGCCGTAATCCCGCTCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtca                                                                                  <  7:561407/151‑1 (MQ=255)
                                                                    aaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGGCGGCGGCCTGCCCCAAATATCGTTAAGATGTTGAGTGATTCTGCATTAGGCTATGGCAAGGTGATCATATTTtct                                                                                  >  1:554837/1‑150 (MQ=255)
                                                                       cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatca                                                                               <  6:805100/151‑1 (MQ=255)
                                                                         gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATAACCTACACATCAAGAGAAAAACTGCAGAAGGATATGGAAAGGAGATAAgcattagatcac                                                                              >  5:1224937/1‑139 (MQ=255)
                                                                           aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgg                                                                           <  3:343955/151‑1 (MQ=255)
                                                                                 tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAt                                                                      <  6:760508/150‑1 (MQ=255)
                                                                                  gCGTTCTGGAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAtt                                                                     <  6:1277564/150‑1 (MQ=255)
                                                                                   ggTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTat                                                                   <  3:139134/150‑1 (MQ=255)
                                                                                   cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATAAAAGGGGGATTat                                                                   >  7:3103/1‑151 (MQ=255)
                                                                                    gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGCCGCCCCCAAATATCGTTCGGCTGATGGGTGTTCCGGGCTTGGGGTGTGGGGAGGTGGTCGGGGTTTGATCCCAggggcgttttg                                                                  >  3:1707595/1‑144 (MQ=255)
                                                                                    gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCGCCTCCCCCAAATATGCTGCAGATGTTGTGTGATGGTGGGTTTGGGGATGGGTAGGGGTGCGGATTTTGTTCACAGGGGCTTTatt                                                                  >  1:696094/1‑150 (MQ=255)
                                                                                         gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGt                                                              <  1:412734/150‑1 (MQ=255)
                                                                                         gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACATCAGATGATGAGTGATCCTGCAGTAGGCTATGGCAAGGTGATCAGATTTTGATCACAGGGGAATTATGATGtt                                                             >  2:358785/1‑151 (MQ=255)
                                                                                           aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATGAGATTTTGATGAAAGGGGAATTATTATTTTa                                                            >  4:11430/1‑150 (MQ=255)
                                                                                            aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                          <  8:824976/151‑1 (MQ=255)
                                                                                              ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATg                                                        <  3:1291371/151‑1 (MQ=255)
                                                                                                 gTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGGCGCCGCCCCCAAATATCCATCAGATGATGAGTGGTGCTGGATTAGGCTATGGGAAGGTGGTGTGATTTTCATAAGAGGGGAAGTATGATGTTATATGCAt                                                     >  1:1702313/1‑151 (MQ=255)
                                                                                                    cAGGAGTAAAACCTGATCCGGCTAATCCGAAAGTAATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTACGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATgg                                                   <  2:1513628/150‑1 (MQ=255)
                                                                                                    cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGc                                                  <  7:1563887/151‑1 (MQ=255)
                                                                                                      ggaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAc                                                <  5:1469572/151‑1 (MQ=255)
                                                                                                        aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATGCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATAACAGGGGAATTATGATGTTAAATGCATGGCAccg                                              >  5:758968/1‑150 (MQ=255)
                                                                                                        aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTACTCCCCCAAAAATACATCAGATGATGAGTGATCCTGCATTATGCTATGTGAAGGTGATCAGATAATAATCACAGGGGAAGAATGATGTTtaaatcaatcaaact                                              >  2:182445/1‑139 (MQ=255)
                                                                                                           aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCGGCCGGCCGCCTCCCCCAAATATCCTTCAGATCATGAGTGATCCTGGATTAGGCTATGGCAAGGTGCTCAGATTTTGATCACAGGGGAATTATGATGTTAAATTAATGGCACCTGcc                                           >  3:1815207/1‑151 (MQ=255)
                                                                                                           aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGGCGCCCCCAAATATCCATAAGATAATTAGTGATCCTGCATTAGGCTATGGGAAGGTGATCTCATGTTAATCTCAGCCGATGTATGATTTTTATTGAATGGCACCGGcc                                           >  3:1526193/1‑151 (MQ=255)
                                                                                                           aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCGCCTCCCCCAAATATACTTCAGATGATGATTGATACTGCATTAGGGTATGGCAAGGTGATCAGATTTTGATAACAAGGGATTTATGATGTTTAATTAATGTCACCTGcc                                           >  1:1591721/1‑151 (MQ=255)
                                                                                                            aaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTACCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATCACAGGGTAATTATGATGTTAAATTCATGGCACCTTCCg                                          >  5:1120670/1‑151 (MQ=255)
                                                                                                               ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGt                                        <  1:190823/150‑1 (MQ=255)
                                                                                                               ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑g                                      <  3:1583500/151‑1 (MQ=255)
                                                                                                                cTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATACTTAAGATGATGAGTGATCCAGCATTAGGCTATGTCAAGGTGATCAGATTTTAATAAAACGGGAATTATGATGTTAAATTAATGGCACCTGCCGGT‑Gc                                     >  6:260427/1‑151 (MQ=255)
                                                                                                                  gATCCGGCTATTCCGAAAGTTATCCGCCCCCGTGCGCCTCACCCAAAAAAAATTCAGATGGTTTGTGATCCTGCATTAGGGGATGGGAATGTGATCAGAGTTTAATCACATGGTAATTATTTTGTTAAATGCATGGCACAGTCCGGT‑Gccc                                   >  8:1342058/1‑151 (MQ=255)
                                                                                                                    tCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAAAATCCTTAAGATGATGAGTGATACTGCATTAGGGTATGGCAAGGTGATCAGATTTTAATAAAAGGGGAATTATGATTTTAAATTAATTGCAACTGCCGGT‑Gccccc                                 >  7:1083261/1‑151 (MQ=255)
                                                                                                                     ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCa                                <  2:330811/151‑1 (MQ=255)
                                                                                                                       ggCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCAtt                              <  2:484093/151‑1 (MQ=255)
                                                                                                                        gCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTAAGATGATGAGTGATCCGGCATTAGGCTATGGGAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGAATGGCACCTGCCGGG‑Gccccccgtt                             >  4:737390/1‑147 (MQ=255)
                                                                                                                        gCTATTCCGAAAGTTATCCGCCCCCCGCCGCCTCCCCCACAAATCCATCAGATGGTGAGTGATCCAGAATTAGCCTATGGCAAGGTGAGCAGAGAATGATCACAGCGGAAATAAGATTTTAAATCCATCGAACCTCCCGGG‑Gcgccgcatt                             >  4:1051035/1‑146 (MQ=255)
                                                                                                                         cTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATACTTCACATTATGAGTCATAATGCATTAGTCTATGGCAAGGTGATAAGATTTTCATAACAGGGGAATTATGATTTTTAATGAATAGCAACAGCCGGT‑GCCCCCATTTg                            >  3:870228/1‑151 (MQ=255)
                                                                                                                         cTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATGCTGCATTAGGGTATGGGAAGGTGATCAGATTTTCATGACAGGGGAATTATTATGTTAAATGAATGTGACCTGCCGGG‑GCCCCCAattt                            >  7:75937/1‑150 (MQ=255)
                                                                                                                         cTATTCCGAAAGTTATCCGCCCCCCTCCACCTCCCCCAAATATACAACAGATGAAGAGTGATCCTGGATTACGGTTTGGCAAGGTGATAAGATATTTATAAAAGCGGAATTATTATTTTAAATTAATGGAACCTGCCGGTCGCCCCAAttt                             >  6:1328521/1‑151 (MQ=255)
                                                                                                                           aTTCCGAAAGTTATCCGCCCCCGGGCGCCGCCCCCAAAAATCCTTCAGATGAGGAGTGATCCTGCATTAGGCTTGGGGAAGGTGATCAGATTTTGATGACAGGGGAATTATTATTTTAAATTAATGGCACCTGACGGT‑GCCCCCATTTGtt                          >  4:968235/1‑151 (MQ=255)
                                                                                                                              ccGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCATTTGTTAAAc                      <  4:1759250/151‑1 (MQ=255)
                                                                                                                               cGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCATTTGTTAAAc                      <  4:1564330/151‑1 (MQ=255)
                                                                                                                                    gTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCATTTGTTAAAcaaagc                 <  1:915502/151‑1 (MQ=255)
                                                                                                                                      tATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCATTTGTTAAAcaaagca                <  1:1416529/150‑1 (MQ=255)
                                                                                                                                         ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCATTTGTTAAACAAAGCAAAGa            <  3:788388/151‑1 (MQ=255)
                                                                                                                                           gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATTATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCATTTGTTAAACAAAGCAAAGAt           >  6:1725412/1‑150 (MQ=255)
                                                                                                                                           gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATAAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCATTTGTTAAACAAAGCAAATATc          >  3:610052/1‑151 (MQ=255)
                                                                                                                                            cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCATTTGTTAAACAAAGCAAAGATCa         <  5:535497/151‑1 (MQ=255)
                                                                                                                                             ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCATTTGTTAAACAAAGCAAAGATCaa        <  8:1558963/151‑1 (MQ=255)
                                                                                                                                             ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCATTTGTTAAACAAAGCAAAGATCaa        <  6:649711/151‑1 (MQ=255)
                                                                                                                                               ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTTCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCATTTGTTAAACAAAGCAAAGATCAACt      <  2:1748279/151‑1 (MQ=255)
                                                                                                                                               ccGCCCTCCTCCCCCAAATTTCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCATTTGTTAAACAAAGCAAAGATCAACt      <  2:641553/151‑1 (MQ=255)
                                                                                                                                                  tcctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCATTTGTTAAACAAAGCAAAGATCAACTGcg   >  8:1615787/1‑150 (MQ=255)
                                                                                                                                                   cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc  >  7:172651/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGT‑GCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTC  >  NC_000913/422283‑422580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑