Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,818,1580TA24.3% 339.9 / 105.0 203intergenic (‑23/+40)argZ/argYtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (64/89);  new base A (14/35);  total (78/125)
Fisher's exact test for biased strand distribution p-value = 1.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 213 total aligned reads displayed.

TAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGAT  >  NC_000913/2818009‑2818308
                                                                                                                                                      |                                                                                                                                                          
tatttCCGCTACCTCTTCGATACCTTTTTTGATGAAGATTTCTTAGTATATGGTGCATCAGTGAGGATTCGAAACTCCGAACGAGCGGTTAGTAGGCGAGTAATATATCCAGCTGAGCTACGGATTCATCGGG‑AAATTTATTTTAatgct                                                                                                                                                            <  4:1155831/147‑1 (MQ=255)
tAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgct                                                                                                                                                            <  1:691914/150‑1 (MQ=255)
 aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgctg                                                                                                                                                           <  1:1762623/150‑1 (MQ=255)
   ttCCTCTACATATTCTATACCTTAATTGCTGAATATTTCGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑t                                                                                                                                                       <  7:1556313/150‑1 (MQ=14)
   ttCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑tt                                                                                                                                                      >  1:319166/1‑150 (MQ=255)
    tCCTCTACCTCTTCCATACCTTCATTGCTGAAGATTTCGGAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑tt                                                                                                                                                      <  4:198156/150‑2 (MQ=14)
     ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑tat                                                                                                                                                     <  3:25557/150‑1 (MQ=255)
     ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCAGA‑‑TAtt                                                                                                                                                    >  2:1608096/1‑151 (MQ=255)
     ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAATTTACTGCAGA‑‑tttt                                                                                                                                                    >  7:1347419/1‑151 (MQ=255)
     ccGCTACCTCTTCGAAACCTTCCTTGCTGAAGATGTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGAACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑tttt                                                                                                                                                    <  6:54823/151‑1 (MQ=14)
      cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTACTGCAGA‑‑ttttt                                                                                                                                                   >  1:17833/1‑150 (MQ=255)
      cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCAGTTCGTATCCGAGTACTAGATCAAGCTCCGCTACGGAGGGATCGTG‑AAATTGATTGTACTGCTTA‑‑AAtt                                                                                                                                                    >  8:321784/1‑150 (MQ=14)
       ggtACCTCTTCGTTCCCTTCATAGCTCAATATTTCGGAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑tttttt                                                                                                                                                  <  6:971765/149‑3 (MQ=11)
       gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTTCTCTATCCAGATGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGATGA‑‑TATT‑g                                                                                                                                                  >  4:383064/1‑150 (MQ=255)
       gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCAGACCGCTCGGTTCGTAGACGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑g                                                                                                                                                  >  3:1746432/1‑150 (MQ=255)
        gttcatctTCGTTACGTTCATTTCTGAATATTTAGTAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑tttttt                                                                                                                                                  <  2:17794/145‑3 (MQ=11)
        cttcatatctgttACGTTAATTTCTGAATATTTAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑ttt                                                                                                                                                     <  5:1817304/135‑3 (MQ=1)
          aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTACTGCAGA‑‑TATT‑tata                                                                                                                                               >  5:38016/1‑150 (MQ=255)
           ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATAcc                                                                                                                                             <  5:1830359/151‑1 (MQ=255)
           ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAATTTACTGCTGA‑‑TATT‑tatat                                                                                                                                              >  4:157462/1‑149 (MQ=255)
             tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTACAACCAAAGCCCCAACCCATACTAGCCAGATACTAAATGCATCGTATATACGAATGCATCAGG‑AAATTTATTTTAATGCTGA‑‑TTTT‑Gagatatc                                                                                                                                           >  5:493675/1‑145 (MQ=2)
              ctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCTGA‑‑tttttttatacct                                                                                                                                           >  3:1038927/1‑142 (MQ=14)
              attCCATACCTTCCTAGATGAATATTTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttgatacc                                                                                                                                            <  8:1731131/149‑9 (MQ=11)
                 cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAGTTTACTGCAGA‑‑tttttttatacctat                                                                                                                                         >  2:706419/1‑139 (MQ=11)
                 cGATACCATCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGctact                                                                                                                                       <  2:248311/151‑1 (MQ=255)
                  gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGcttct                                                                                                                                       >  7:856157/1‑150 (MQ=255)
                  gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGctact                                                                                                                                       >  2:797442/1‑150 (MQ=255)
                  gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCAGA‑‑TTTA‑GATaggaatacc                                                                                                                                       >  6:1809951/1‑142 (MQ=14)
                   aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTTCTaa                                                                                                                                     <  8:813255/151‑1 (MQ=255)
                   aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTaa                                                                                                                                     <  1:1028442/151‑1 (MQ=255)
                   aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTACTGCAGA‑‑TATT‑tatatacctataa                                                                                                                                      >  7:1425802/1‑141 (MQ=11)
                    atcgttcattcaTGAATATTTAGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGctact                                                                                                                                       <  4:1408523/146‑1 (MQ=14)
                     aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTaaa                                                                                                                                    <  4:760311/150‑1 (MQ=255)
                      cgttCATTTATGAATATTTAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGCTACTaaa                                                                                                                                    <  7:1038412/149‑1 (MQ=14)
                      ccTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGc                                                                                                                                  <  6:75776/151‑1 (MQ=255)
                       tttCTTTGTTTAAGTTTTGGGAGAATATGTTGCTTCCGGTTGTATTCTAACCTCGTACGGGTCGGTTGGTAGCGTAGTACTCTATCCAGCTGAGCTAGGTATGTATGGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATAGCGCTACTAAAGc                                                                                                                                  <  7:548385/149‑1 (MQ=255)
                       catCATAGCTGAATATGTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACAGCTACTAAAg                                                                                                                                   <  3:1348168/149‑1 (MQ=11)
                       cTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCAGA‑‑TTTT‑TATATCggaagagaacac                                                                                                                                 >  3:1413794/1‑140 (MQ=14)
                         taaTTTCTGATGATTTAGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGcc                                                                                                                                 <  7:1530339/147‑1 (MQ=14)
                         taaTTGCTGAATATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGCTACTAAAGc                                                                                                                                  <  5:716001/148‑1 (MQ=11)
                         tCATTGCTGAAGATTTCGGAGAATATGTTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCa                                                                                                                                <  7:1591028/150‑1 (MQ=255)
                          cATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCata                                                                                                                              <  8:1058155/151‑1 (MQ=255)
                          cATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCata                                                                                                                              <  1:1422350/151‑1 (MQ=255)
                          cATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCata                                                                                                                              <  3:1660188/151‑1 (MQ=255)
                          aattaatGAATATTTAGTATAATATTGTGCATCCGTGAGGATTAGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATAGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGCTACTAAAGcc                                                                                                                                 <  7:8138/144‑1 (MQ=14)
                              gatGAATATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGCTACTAAAGCCGTATc                                                                                                                            <  6:1071623/149‑1 (MQ=11)
                              gCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCa                                                                                                                           <  5:1572056/150‑1 (MQ=255)
                               atccataTTTAGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGCTACTAAAGCCGTATCa                                                                                                                           <  7:1092099/145‑1 (MQ=14)
                                ggAAGATCTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGCTACTAAAGCCGTATCaa                                                                                                                          <  5:1155943/150‑1 (MQ=14)
                                 gAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAg                                                                                                                         <  3:700797/149‑1 (MQ=18)
                                 gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGt                                                                                                                        <  1:807635/150‑1 (MQ=255)
                                 gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGt                                                                                                                        <  6:1282670/150‑1 (MQ=255)
                                 gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGt                                                                                                                        >  5:1651823/1‑150 (MQ=255)
                                  aaGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTaa                                                                                                                      <  6:1645692/151‑1 (MQ=255)
                                      tttCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCTGA‑‑TTTT‑GTTAACGCTAATATAGCCAAATCAAATAag                                                                                                                     >  5:18850/1‑148 (MQ=11)
                                        tCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGAt                                                                                                                 <  1:405195/150‑1 (MQ=40)
                                           gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTg                                                                                                             <  3:1103660/151‑1 (MQ=39)
                                           gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCAGA‑AATTAAAATATACTGCTAA‑‑TAAA‑AATAAATATACTAAAGACAAATAAAGTaatatagact                                                                                                              >  4:707222/1‑142 (MQ=2)
                                             caagagggcgCAGCCGGCAGGATGCGAACCTCCGACCGCCCGGTGCGTATTTTATTAATCTATAACCCTGACCTAGCGATTCATCGGG‑AATTTTTTTTTACTGAGGT‑‑TATT‑GATTCCGATATTAAAGCCATATCAAGTAAGAGATGGTGCa                                                                                                           <  3:852693/142‑1 (MQ=9)
                                              aaTATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCAt                                                                                                          <  6:1638504/151‑1 (MQ=39)
                                                 atGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATATAATTTACTTCATA‑‑TAAT‑TATAACGCAACTAAAGCCATATAAAATAag                                                                                                                     >  5:1380809/1‑137 (MQ=11)
                                                       cATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCTGA‑‑TTTT‑TAGACCCCTCGTAAAACCATATATAGTAACAGATgatcgctccagcaccc                                                                                                 >  6:183403/1‑136 (MQ=11)
                                                          ccGGGAGTATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGATCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGAtt                                                                                               <  4:97597/150‑1 (MQ=255)
                                                          ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGCGCTACGTATGCATCGGG‑AAATTTTTTTTACTGCTGA‑‑TATT‑GATACCGCTAATAAAGCCATATCAAGTAACAGATGGTGCATCCGGGAGGAtt                                                                                               <  5:706744/150‑1 (MQ=255)
                                                           cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑TATAAAGCTAAAAAAGACATATCAAGTAAGAGATGGTGCGGCCGGGAGGAtt                                                                                               >  4:1469628/1‑149 (MQ=14)
                                                                ggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑TATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGAttatacgcg                                                                                        >  1:281716/1‑144 (MQ=255)
                                                                 gaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGacgcgc                                                                                       >  1:1304285/1‑146 (MQ=255)
                                                                  aTTCGAACCTCCGACCGCTCGGTTCATATCCGAATACACTATACAACTGAAATACTGATGCATATAG‑AAAATTAATATAATGCAGA‑‑AATT‑TAAAAAAATAAAAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGGTTAGACCCGcc                                                                                      >  4:466049/1‑151 (MQ=2)
                                                                   ttCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTACTGCTGA‑‑TATT‑TATACAGCTACAAAAGCCATATCAAGTAATAGATGTTGAATACGGGAGGAttatacgctccg                                                                                     >  1:148870/1‑141 (MQ=11)
                                                                      gAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑ta                                                                                                                                                      <  5:651259/84‑1 (MQ=255)
                                                                      gAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTAAAAAAGCCATATCAAGAATTAGATTGTGCAACCGCGTGGATTAGacacgaatagc                                                                                  >  3:218512/1‑141 (MQ=14)
                                                                       aaCCTCCGCCCGGGCGGGTCGTAGCGGAGTACGCTATCCAGCTCAGCATCGGATGCATCGGG‑AAAGTTATTTTACTGGTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCg                                                                                <  8:528910/151‑1 (MQ=17)
                                                                             cGAGCGCTCGGGTCGTAGCCGAGGACTCAATACAGCACCTCTTCCTACGAAGCGGG‑AACTTTAAATTACTCCTCA‑‑TATT‑GCAACCCCTAATACAGCAATATCAAGTACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGC‑CCGCTCGGt                                                                          <  6:246988/151‑1 (MQ=12)
                                                                              gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACAGCTACTAAAGCCATATCAAGTTAGAGATGGTGCATCCGGGAGGATTAGACGCTCCGA‑GCGGTccgat                                                                         >  3:1845101/1‑147 (MQ=14)
                                                                               ccggcgcgcttCTTAGCGGAGTACTCACTCCAGCGGTGCTACGTACCCATCTGC‑ACATTTATATTCCTCCAGA‑‑TCTT‑CCATCCGCTACTAGCGCCATATGTAGGACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTc                                                                        <  3:971682/142‑1 (MQ=9)
                                                                                ccGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATAACGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACGCGCCGA‑CCGcaccgatcg                                                                       >  3:833997/1‑143 (MQ=14)
                                                                                     ggtttCGTATCCGAGTACTCTAACAAGCTGAGATACTGATGCATCGGG‑ATATTTATTTTACTGTTGA‑‑TATT‑GTAACCGATAATATAGCCATATCTAGTAAAATATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGcc                                                                  <  4:629460/148‑1 (MQ=18)
                                                                                      ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCg                                                                 <  8:382650/151‑1 (MQ=255)
                                                                                        ttCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACCCTCCGA‑CCGCACCGGTCGTAGCCg                                                                 >  1:1207836/1‑149 (MQ=255)
                                                                                          cGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGAATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATAACCCTAATAAAACCAAATAAAGTAAGAGATGGTGCATCCGGGAGGATTCGAAACTCCGC‑GCGGGGGGGTCGTCGCCGAGtt                                                             >  4:279656/1‑150 (MQ=14)
                                                                                             aGCCGAGTACTCTATCCAGATGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTActc                                                          <  8:1616344/151‑1 (MQ=255)
                                                                                              gCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAACCTCAGA‑CCGATCGCGACGTAGCCCAGTaaaa                                                          >  8:1196556/1‑147 (MQ=17)
                                                                                                        ctATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGTTGCATCAGGGAGGATTAGACGCGCCGAGCCGCTC‑GCTCCTCGCCGAGTagataaaccagcttt                                               >  6:714732/1‑137 (MQ=14)
                                                                                                        ctATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAACCTCCGA‑CCGCTCCGATCGTAGCCGAGTAcagataacaaa                                                  >  2:146254/1‑138 (MQ=18)
                                                                                                          aTCAAGCTCGGCTACGTCGGCATCGGG‑TAATTTTTTTTCCTCCTGA‑‑TATT‑CATACCGGTCATACAGCAATATCAAGTACGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                                <  8:450446/148‑1 (MQ=17)
                                                                                                             catgttatcTAAGGTTGGATCGGGTAAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGa                                                                                            <  5:829459/95‑1 (MQ=11)
                                                                                                                  gctttacgtagccagcGGG‑AGAGTTAATTTACTGTTCC‑‑TCTT‑GTTAACCCTACTTCAGGAATATCACTTAATAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGAt                                      <  1:786101/135‑1 (MQ=2)
                                                                                                                  gAGCTACGGATGCATCGGG‑AAATGTAAATAACGTCTGA‑‑TATT‑GAAACAGATAATAAAAAAATATAAATAAAGAGATGGTCCATAGCAGAAGAATAAAAACTCCGA‑CCGCTCGGTTCGTAGCCGAGGGCTCTATCCAGCTGAGCTACGGATg                                     >  2:361065/1‑151 (MQ=9)
                                                                                                                    gCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCCAACCTCGGC‑CCCTCCGGTTGGTCGCCGATTAATCTAACCAGTTCAGATACGTAt                                      >  1:58471/1‑149 (MQ=11)
                                                                                                                       tctgATGCATCGTT‑AATTTTATTTTACTGCTTA‑‑CATT‑CATACAGCTGCTAACTCTTTATAAATTACTATATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATc                                 <  8:706912/147‑1 (MQ=14)
                                                                                                                         ggATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAACCTACGA‑CCGAACCGTTCCTAGCCGAGTACTCTATCCAGCTGAGCTACCGATGCATCggg                              >  4:90187/1‑151 (MQ=21)
                                                                                                                         cgATCCAACGGG‑AAATCTATTATACTGCTCA‑‑TACT‑GCTACCGCTACTCAAGCCATATCAATTAATAGATGGTGCATCCGGGATCCTTCGAAACTCCGC‑GCGCTGGGATCGCAGACGAGTACTCTATCCAGCTGAGCTACGGATGCATCggg                              <  1:359804/150‑1 (MQ=16)
                                                                                                                                     aaaaGTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttacttt                   <  8:1235845/150‑1 (MQ=255)
                                                                                                                                       aacttattcTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTc                                                                                       <  6:1479878/79‑1 (MQ=255)
                                                                                                                                         tttatttTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCCATCCGTTAGTAGCCGAGTACTATATCCAGCTGAGCTACGGATGCATCGGGAAAGTTACTTTACTg               >  1:1813515/1‑151 (MQ=21)
                                                                                                                                         cttattcTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTcc                                                                                      <  1:336183/80‑1 (MQ=255)
                                                                                                                                            aatttACTGCTCA‑‑TATT‑GCTACCGCTAATACCTCAATATCTCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCa             <  1:1501828/148‑1 (MQ=11)
                                                                                                                                              tctACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTc                                                                                              <  1:454980/66‑1 (MQ=17)
                                                                                                                                                 aCTGCTGC‑‑TATTGGATACC‑ATACTTGAGCCATATCTAGGAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAttt        <  8:1796010/150‑1 (MQ=17)
                                                                                                                                                  ctgctgA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTACACCCTCAGA‑CCCAACGCTTAGTAGCCGATTAGTCTATCCAGATGAGCTACGGATGCAGCGGGAAAATTACTTAACTGAAGAtattt      >  2:892177/1‑151 (MQ=18)
                                                                                                                                                      |                                                                                                                                                          
TAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGAT  >  NC_000913/2818009‑2818308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑