Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,947,4650T.24.5% 391.3 / 131.9 152intergenic (+2/‑32)metZ/metWtRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (21/93);  new base . (6/31);  total (27/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

Only 100 of 169 total aligned reads displayed.

TGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCG  >  NC_000913/2947315‑2947549
                                                                                                                                                       |                                                                                      
tGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCTGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt                                                                                        >  7:1532276/1‑151 (MQ=255)
tGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt                                                                                        >  1:1223375/1‑151 (MQ=255)
agCCTAATCCCTGATCGTGTT‑AAAAAAACGGCTGAATGAATAATATATCGTAGTATAGATCGATTACACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt                                                                                        <  2:421146/150‑1 (MQ=9)
 gCCTAATGCGTTATCGTGTT‑AAAAATAAGGTTGAATGAAAAAGCTATCGGAGTAGACCCCGAATACACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAtc                                                                                       <  7:682079/151‑2 (MQ=14)
 gCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt                                                                                        <  1:978706/150‑1 (MQ=255)
 gCCAAATCCGTGAGCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAtt                                                                                       <  6:39228/151‑1 (MQ=255)
  cctaaTCCGTGTTCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTa                                                                                      <  6:115756/148‑1 (MQ=255)
   cAAATCCGTGATCTGGGT‑AAAATCCAGGCTGCATGAAAACGCGAGTGCAGTATAGTCGCCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaa                                                                                     <  7:1154746/151‑1 (MQ=17)
   cAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAtaa                                                                                      >  3:73207/1‑148 (MQ=255)
   cAAATCCGTGAGCTGGTT‑ATAATTAAGGTTGAATGAATACGCCAGCGGAGAAGACTCCGCTTCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaa                                                                                     <  7:916425/151‑1 (MQ=14)
    aaaTCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAACTCCGGCCCCCGCAACCAATaaa                                                                                     >  8:416988/1‑150 (MQ=255)
    aaaTCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaa                                                                                    <  6:745139/151‑1 (MQ=255)
    aaaTCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaa                                                                                    <  5:1723071/151‑1 (MQ=255)
      aTCGGTGATCGTGGT‑AAAATTAAGGTTGCATGAAAACTAGTGCGGAGTATAGTGCGCATCCCGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaatt                                                                                  <  2:1226543/151‑3 (MQ=17)
       tCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAAtt                                                                                 >  8:1442682/1‑151 (MQ=255)
        gtgTGATCGGGTT‑ATAAATAAGGTTGAATGAATTATAGAGTGTAGTATATTGCGATTCCTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAtttg                                                                                <  5:22003/149‑2 (MQ=11)
        gggtgcgcGGGTT‑AAAAACAAGGTTGCATGAAAACGCGAGCGGAGTATAGTTCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaac                                                                                <  6:1828395/144‑7 (MQ=18)
        ccGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAttt                                                                                <  4:1740999/151‑1 (MQ=255)
         cGTGCTCGGGGT‑AAAAAAAAGGTTGCAGGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTGCAAATCCGGCCCCCGCAGCCAATTAAAATTtg                                                                               <  1:1296456/151‑1 (MQ=255)
         cGTGATCGGGGTAAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAtttt                                                                                >  7:641208/1‑151 (MQ=255)
         cGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtg                                                                               <  5:1505541/151‑1 (MQ=255)
         cGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtg                                                                               <  5:299828/151‑1 (MQ=255)
         cGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCT‑GTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGAAAAttgat                                                                              >  8:556481/1‑148 (MQ=255)
           ggATCGGGTT‑AAAATACCGGGTGCATGAAAACGCGAGCGGAGTATACTGCGCATCCCCGGACGCGGGGTGGAGCTGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaacacc                                                                             <  3:1531544/150‑10 (MQ=17)
            gctctggtt‑AAAAAAAAGGTTGCATGAAAACCCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTtgatga                                                                           <  5:1147285/143‑1 (MQ=18)
            gATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgatg                                                                            <  6:579446/151‑1 (MQ=255)
            gATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgat                                                                             <  7:1417587/150‑1 (MQ=255)
            gATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TAAAATTtga                                                                              >  4:529832/1‑148 (MQ=255)
             aTCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCCGCCGGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGGTCAACTCCGGCCCCCGCACCCAA‑TAAAATTtggtggc                                                                          >  8:1484113/1‑149 (MQ=21)
             aTCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgatga                                                                           <  8:167793/151‑1 (MQ=255)
               gggggT‑AAAATAAAGCCTGAATGAAATCTAGAGCGGAGTATACTGCCCATCCTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑t                                                                       <  2:77562/150‑1 (MQ=14)
               cGGGGT‑AAAAAACCCGCTGCAGGAAAAAGAGAGCGGAGAATAGAGCGCATCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGaa                                                                          <  1:675544/149‑1 (MQ=14)
               cGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaattaacaaccaa                                                                          >  4:1607931/1‑139 (MQ=255)
               cGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGaa                                                                          <  6:1724297/150‑1 (MQ=255)
                ggggT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑t                                                                       <  7:888879/151‑1 (MQ=255)
                ggggT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑t                                                                       <  1:1035409/151‑1 (MQ=255)
                gggcT‑AACAAAAAGGCTGCATGAAAACGCGAGCGGAGTATAGTCCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑T‑a                                                                     <  1:1715437/151‑1 (MQ=17)
                 gggT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaacaccctaacg                                                                       >  1:1626566/1‑136 (MQ=255)
                 gggT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑a                                                                     <  2:698749/151‑1 (MQ=255)
                 gggT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑a                                                                     <  2:951581/151‑1 (MQ=255)
                  ggT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaatgaacaccctaccgg                                                                      >  6:999860/1‑136 (MQ=255)
                  ggT‑AAAAAAAAGGTTGCATGAAAACGAGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTAGTCGGTCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCAGCAACCAATTattgaacaaactaccg                                                                       >  8:234390/1‑135 (MQ=18)
                   gT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑aaa                                                                   <  7:405216/151‑1 (MQ=255)
                   gT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑aaa                                                                   <  1:472246/151‑1 (MQ=255)
                   gT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑aaa                                                                   <  3:1109267/151‑1 (MQ=255)
                   gT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑aaa                                                                   <  3:530777/151‑1 (MQ=255)
                   gT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑aaa                                                                   <  4:112711/151‑1 (MQ=255)
                   gT‑AAAAAAAACGTTCCATGAAAACTCTAGCGGAGAATAGTGCGCATCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑T‑aaaa                                                                  <  6:1070165/151‑2 (MQ=17)
                      aaaaaaaaGGTTGGATGAAAACGCGAGCGGAGTATAGTGCGCATGCACGGACGCGGGGTGTAGCAGCCTGGTAGCTCGTCGGGCTCATTACCGTAAGGTCGTCGGTGCAAATCCGGCCGCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGc                                                                 <  4:1412177/151‑1 (MQ=21)
                      aaaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGc                                                                 <  7:830337/151‑1 (MQ=255)
                      aaaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGc                                                                 <  7:1007507/151‑1 (MQ=255)
                      aaaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGc                                                                 <  3:920472/151‑1 (MQ=255)
                       aaaaaaaGGTTGCATGAAAACGCGAGCGGAGTAGATAGCGCATACCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑T‑aaaagca                                                               <  6:1686625/151‑5 (MQ=14)
                        aaataaGGTTCCATGAATTCTCTATCGTAGTATTGTGCGCTTCCTGGGACGCGTTGTGGAGGAGCCTGGTAGCTCGTCGGGCTCATTACCCGAAGGTCGTCGGTTCAAATCCGGCCCGCGCAACCAA‑TCAAATTTGATGAAG‑T‑aaaaggag                                                              <  7:419052/151‑6 (MQ=2)
                        aaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAg                                                               <  8:1044083/151‑1 (MQ=255)
                        aaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAg                                                               <  4:1198645/151‑1 (MQ=255)
                        aaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAg                                                               <  7:849145/151‑1 (MQ=255)
                        aaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTT‑ATGAAGTT‑AAAGc                                                                 >  4:1608671/1‑149 (MQ=255)
                        aaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TAAAATTTGATGAAG‑T‑aaaagc                                                                >  7:586067/1‑146 (MQ=255)
                         ggcgatcttGCAGGAAAACGAGAGCGGAGTATAGTCCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑TAAAAGCAGt                                                              <  8:1823583/144‑1 (MQ=14)
                         aaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGt                                                              <  6:1463318/151‑1 (MQ=255)
                          aaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTa                                                             <  3:145441/151‑1 (MQ=255)
                           aaaGGCTGCATGAAAACGAGAGCGTAGTATAGTGCGCATCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑TAAAAGCAGTAc                                                            <  3:586064/151‑1 (MQ=14)
                             aGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACgg                                                          <  6:1056489/151‑1 (MQ=255)
                               ggtccATGAAAAAGCGAGCGTAGTATAGTGCGCATCCTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑TAAAAGCAGTACGGTg                                                        <  4:1443815/147‑1 (MQ=14)
                                 tGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑TAAAAGCAGTagatagg                                                       >  4:1824015/1‑144 (MQ=17)
                                  gCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGAcg                                                     <  4:880969/151‑1 (MQ=255)
                                  gCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGAcg                                                     <  4:1452841/151‑1 (MQ=255)
                                  gCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGAc                                                      <  8:136942/150‑1 (MQ=255)
                                  gCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TAAAAATTGATGAAT‑TAAAAGCAGTAc                                                            >  5:1043700/1‑144 (MQ=17)
                                      gaatacgcttggggtgTATAGTGCGCCTCCTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑TAAAAGCAGTACGGTGACGCggg                                                 <  2:172095/136‑1 (MQ=11)
                                      gAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCggg                                                 <  2:1524434/151‑1 (MQ=255)
                                       aaaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt                                                                                        >  6:641385/1‑113 (MQ=255)
                                       aaaaCGAGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt                                                                                        <  5:643422/113‑1 (MQ=255)
                                        aaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGt                                               <  3:857033/151‑1 (MQ=255)
                                        aaaCGCCAGGGGAGTATAGTTGGAATCCAGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCGCGCAACCAA‑TCAAATTTGATTAAG‑TAAAAGCAGTACGGTGACGCGGGGt                                               <  1:67313/151‑1 (MQ=11)
                                         aaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTg                                              <  7:1587975/151‑1 (MQ=255)
                                         aaCGCGAGCGGAGTATAGCGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTg                                              <  8:604336/151‑1 (MQ=255)
                                          aCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTgg                                             <  1:280707/151‑1 (MQ=255)
                                           cgcgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGa                                            <  3:1302821/151‑1 (MQ=255)
                                           cgcgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGa                                            <  2:1389218/151‑1 (MQ=255)
                                           cgcgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGa                                            <  5:748016/151‑1 (MQ=255)
                                            gcgAGCGGAGTATAGTGGGCATCCACGGTCGCGGGGTGGAGCAGCCTGGTAGCTCGTAGGGCTCATAACCCGAAGGTCGTCGGTTCTAATCCGGCCGCCGCAAGCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGag                                           <  4:385928/151‑1 (MQ=18)
                                            gcgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGGAGGGCTCATAACCCGAAGGTCGGCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGag                                           <  1:1328119/151‑1 (MQ=255)
                                             cgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGTCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGagc                                          <  1:1748487/151‑1 (MQ=255)
                                              gatcGGAGTATAGTGCTCATCCTCGGACTCTGGGTGGAGCAGCCTGGTAGCTCGTCGGTCTCATAACCCGAAGGTCTTCGGTTCAAATCCCGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACTGTGACGCGGGGTGGagc                                          <  7:241722/147‑1 (MQ=17)
                                              gAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGagca                                         <  8:1589495/151‑1 (MQ=255)
                                                    aGTATACGGCGCATCCACCGACGCGGGGTGGAGCAGGGTGGTAGCTCTTCGGGCTCATAACCCGAATGTCGTCGGGTCAAATCCGGCCCCCGCAACCAATTAAAATTTGCTGAAG‑T‑AAACCAGTACGGTGACGCGGGGTGGAGCAGCCTgg                                   <  3:375915/151‑1 (MQ=17)
                                                     gTATAGTCCCCATCCACCGAACAGGTGTGGATCAGGCTTGTAGCTCGTCTGTCTCATAAGCCTAAGGGGGGCTGTTCAAATCATGCCCGCGCAACCAATTAAATTTTGATGAAT‑T‑AACGCACTACCCTGACGCGGGGTGGAGCAGCCTGGt                                  <  8:578232/151‑1 (MQ=9)
                                                         aGTGCGCATCCAGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt                                                                                        <  6:132908/95‑1 (MQ=255)
                                                         aGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt                                                                                        >  5:132985/1‑95 (MQ=255)
                                                          gTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGGCGACGGTTCACATCCTGCCCCCGCAACCAATAAAAATTTGTTTAAG‑T‑AAAGCAGTACGGTGACGCCGTGTGCAGCAGCCTGGTAGCtc                             >  4:1331116/1‑151 (MQ=17)
                                                          cggctcATCCCCGGACGAGGGGGGGAGCAGGCTGGTCGCTGTGCGGCCAATTAACCCGAATGGCGGCGCGGCAAAACCCGCCCCCGCAACCAAATACACTGCGCTGAAG‑A‑CACGCACTCCCGGGACGCGGGGTGGAGCAGCCTGGTAGCtc                             <  3:1024949/146‑1 (MQ=255)
                                                                aTCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAGGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGagca                                         <  8:1602769/133‑1 (MQ=255)
                                                                  ccACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattg                                                                                   >  5:1434336/1‑88 (MQ=14)
                                                                     cGGACGCGGGGTGGAGCAGCCTGTTAGCTCTTCGCTAACATAACCCGAAGGTCGTCTGGGCAAAACACCCCGCCGCAACCAATTAAAATTCGATGAAG‑A‑AACCCACTACGGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCAt                  <  8:77280/151‑1 (MQ=9)
                                                                                  gaggagCGTGGTATCTGTTCGGGATCATATCCCTAATGTCGTCGGTTCAAATCATGCCGCCGCAATCAATTAAACTTTGCTGTAT‑T‑AAAGGACTCCCGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcg     <  1:890542/151‑1 (MQ=2)
                                                                                                                             ggTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGtcgtcg  >  3:262509/1‑111 (MQ=255)
                                                                                                                                          cccccGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAcc              >  2:969730/1‑86 (MQ=255)
                                                                                                                                          cccccGCAACCAATTAAAATTTCATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAcc              <  1:967893/86‑1 (MQ=255)
                                                                                                                                                       |                                                                                      
TGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCG  >  NC_000913/2947315‑2947549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑