BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,587,531	0	G	A	56.8%	180.0 / inf	450	L195F (CTC→TTC)	ugpQ	glycerophosphodiester phosphodiesterase, cytosolic
Reads supporting (aligned to +/- strand): ref base G (99/95); new base A (111/144); total (210/240)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 450 total aligned reads displayed.

AAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACA  >  NC_000913/3587382‑3587680

   gTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAAc                                                                                                                                                   >  5:113091/1‑151 (MQ=255)

    ttCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACt                                                                                                                                                  >  3:1670298/1‑151 (MQ=255)

    ttCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACt                                                                                                                                                  >  5:608850/1‑151 (MQ=255)

      cGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACtt                                                                                                                                                 >  6:415521/1‑150 (MQ=255)

      cGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACtt                                                                                                                                                 <  8:1431620/150‑1 (MQ=255)

         aCCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGa                                                                                                                                             <  1:1327156/151‑1 (MQ=255)

           cAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGAtt                                                                                                                                           >  2:419923/1‑151 (MQ=255)

            aaTCACGTCAATCGCATCTGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGTGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCTGCGTCTTTCAACTGCATCACTCGCGCTTTATTGTGTAACTTATGATTg                                                                                                                                          <  3:116692/151‑1 (MQ=255)

            aaTCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTg                                                                                                                                          <  5:409705/151‑1 (MQ=255)

             aTCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTg                                                                                                                                          >  3:746729/1‑150 (MQ=255)

             aTCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTga                                                                                                                                         <  7:1186616/151‑1 (MQ=255)

               cACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGTGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATTATTgag                                                                                                                                        <  4:1243627/150‑1 (MQ=255)

                aCGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTg                                                                                                                                          >  2:295167/1‑147 (MQ=255)

                aCGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGAt                                                                                                                                      <  8:218823/151‑1 (MQ=255)

                aCGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGAt                                                                                                                                      <  4:171195/151‑1 (MQ=255)

                 cGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATg                                                                                                                                     <  2:1354766/151‑1 (MQ=255)

                    cAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGaa                                                                                                                                   <  6:609606/150‑1 (MQ=255)

                    cAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGaa                                                                                                                                   <  1:412912/150‑1 (MQ=255)

                    cAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAAt                                                                                                                                  <  8:1612597/151‑1 (MQ=255)

                      aTCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATat                                                                                                                                >  7:402064/1‑150 (MQ=255)

                        cGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATagag                                                                                                                              <  6:170008/151‑1 (MQ=255)

                         gCATCTGTACAGTTTCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATagag                                                                                                                              <  4:1516522/150‑1 (MQ=255)

                         gCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAgaga                                                                                                                             <  1:63840/151‑1 (MQ=255)

                          cATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGAc                                                                                                                            <  4:811630/151‑1 (MQ=255)

                           aTCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACg                                                                                                                           >  7:643844/1‑151 (MQ=255)

                           aTCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACg                                                                                                                           >  7:1458862/1‑151 (MQ=255)

                           aTCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACg                                                                                                                           <  5:1431591/151‑1 (MQ=255)

                           aTCGGTACAGAGGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACg                                                                                                                           >  3:830441/1‑151 (MQ=255)

                                tACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGcagcc                                                                                                                      >  7:190390/1‑151 (MQ=255)

                                tACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGcagcc                                                                                                                      >  7:307372/1‑151 (MQ=255)

                                 aCAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGcagccc                                                                                                                     <  1:790521/151‑1 (MQ=255)

                                 aCAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGcagccc                                                                                                                     >  6:1439581/1‑151 (MQ=255)

                                    gATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGcagcccagc                                                                                                                  >  8:858907/1‑151 (MQ=255)

                                    gATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGcagcccagc                                                                                                                  >  3:1520324/1‑151 (MQ=255)

                                         aaTCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCcgcgc                                                                                                             >  4:316987/1‑151 (MQ=255)

                                             cacaCCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTc                                                                                                         <  2:1429402/151‑1 (MQ=255)

                                              acacCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTc                                                                                                         >  7:1072255/1‑150 (MQ=255)

                                                acCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGt                                                                                                      <  6:728175/151‑1 (MQ=255)

                                                       cgcAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGcc                                                                                               >  2:1614845/1‑151 (MQ=255)

                                                         cagcaACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAg                                                                                             <  3:1461224/151‑1 (MQ=255)

                                                          agcaACTCTGCTGCGCGCTTGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAgt                                                                                            <  2:1309594/151‑1 (MQ=255)

                                                           gcaACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAgtc                                                                                           <  6:506848/151‑1 (MQ=255)

                                                           gcaACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAgtc                                                                                           <  1:1609828/151‑1 (MQ=255)

                                                             aaCTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAgtcgt                                                                                         <  4:604604/151‑1 (MQ=255)

                                                              aCTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAgtcgtc                                                                                        <  7:162661/151‑1 (MQ=255)

                                                              aCTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCACGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAgtcgtc                                                                                        <  2:736301/151‑1 (MQ=255)

                                                                tctGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTcgc                                                                                      >  8:1202405/1‑151 (MQ=255)

                                                                  tgctgcGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCgcgc                                                                                    >  2:969622/1‑151 (MQ=255)

                                                                  tgctgcGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCgcgc                                                                                    >  7:770342/1‑151 (MQ=255)

                                                                        gcgcTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCa                                                                              <  4:1109333/151‑1 (MQ=255)

                                                                             ggggTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAg                                                                         <  7:992564/151‑1 (MQ=255)

                                                                               ggTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGca                                                                       <  3:64030/151‑1 (MQ=255)

                                                                                 tttATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGcaac                                                                     >  3:13454/1‑151 (MQ=255)

                                                                                    aTTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGcaacaa                                                                   >  8:437980/1‑150 (MQ=255)

                                                                                      tGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCCGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAAc                                                                 <  1:1044440/150‑1 (MQ=255)

                                                                                       gACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACcg                                                               >  2:879235/1‑151 (MQ=255)

                                                                                          ggTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACcgcgc                                                            <  3:2848/151‑1 (MQ=255)

                                                                                          ggTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACcgcgc                                                            <  1:827765/151‑1 (MQ=255)

                                                                                             ataAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCgg                                                          <  4:745663/150‑1 (MQ=255)

                                                                                                aaCCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCCACAAACCGCGCGGCAGt                                                      <  1:904067/151‑1 (MQ=255)

                                                                                                aaCCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGt                                                      <  2:51689/151‑1 (MQ=255)

                                                                                                 aCCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGtt                                                     <  5:18157/151‑1 (MQ=255)

                                                                                                  ccAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTc                                                    <  7:558441/151‑1 (MQ=255)

                                                                                                  ccAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTc                                                    >  7:1315563/1‑151 (MQ=255)

                                                                                                   cAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTcc                                                   >  4:966230/1‑151 (MQ=255)

                                                                                                    aGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCg                                                  <  2:613848/151‑1 (MQ=255)

                                                                                                      aaTCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGt                                                <  6:1304039/151‑1 (MQ=255)

                                                                                                        tCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTgc                                              >  5:887420/1‑151 (MQ=255)

                                                                                                         ccGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTgcc                                             <  8:1289672/151‑1 (MQ=255)

                                                                                                           gCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTgccgc                                           >  3:546366/1‑151 (MQ=255)

                                                                                                           gCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTgccgc                                           <  4:471992/151‑1 (MQ=255)

                                                                                                             aGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCt                                         <  4:293470/151‑1 (MQ=255)

                                                                                                              gTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCtg                                        >  5:1247559/1‑151 (MQ=255)

                                                                                                                  ggCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCtgttgt                                    >  1:210567/1‑151 (MQ=255)

                                                                                                                  ggCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGAAGCAGCCAAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGCTGCCGCCtgttgt                                    >  1:1420631/1‑151 (MQ=255)

                                                                                                                    cGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGc                                  >  8:63738/1‑151 (MQ=255)

                                                                                                                    cGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGc                                  <  1:546923/151‑1 (MQ=255)

                                                                                                                    cGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGc                                  >  5:1520519/1‑151 (MQ=255)

                                                                                                                    cGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTAAGATTGAGATGAAAAGAGACGCAGCCCAGCCGCGCGGTCAGTTCCCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGc                                  <  2:1099511/151‑1 (MQ=255)

                                                                                                                       cTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGc                               >  1:1236113/1‑151 (MQ=255)

                                                                                                                         ttCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGc                               <  5:337743/149‑1 (MQ=255)

                                                                                                                         ttCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTGGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCtt                             >  6:310379/1‑151 (MQ=255)

                                                                                                                          tCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTc                            >  3:805696/1‑151 (MQ=255)

                                                                                                                           cAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCt                           <  1:289763/151‑1 (MQ=255)

                                                                                                                            aaCTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCt                           >  6:910649/1‑150 (MQ=255)

                                                                                                                            aaCTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCt                           >  2:333140/1‑150 (MQ=255)

                                                                                                                            aaCTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCt                           >  5:1662519/1‑150 (MQ=255)

                                                                                                                              cTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTaa                         >  2:1167121/1‑150 (MQ=255)

                                                                                                                                   tCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCAt                    >  8:898988/1‑150 (MQ=255)

                                                                                                                                     aCTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCaa                 <  5:460152/151‑1 (MQ=255)

                                                                                                                                     aCTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCaa                 <  8:1073992/151‑1 (MQ=255)

                                                                                                                                     aCTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCaa                 <  6:1170521/151‑1 (MQ=255)

                                                                                                                                        cgcgCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGAGCCACTCATCCAGCAACAACCCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAAtct              <  7:470668/151‑1 (MQ=255)

                                                                                                                                         gcgcTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAAtctc             >  8:55873/1‑151 (MQ=255)

                                                                                                                                          cgcTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAAtctc             >  6:1080308/1‑150 (MQ=255)

                                                                                                                                            cTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCCGCTTCTAAAGCATCAATCTCaa           >  4:1351699/1‑150 (MQ=255)

                                                                                                                                            cTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCaa           >  3:38640/1‑150 (MQ=255)

                                                                                                                                                  tGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATg     <  8:374382/150‑1 (MQ=255)

                                                                                                                                                  tGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATg     >  7:709594/1‑150 (MQ=255)

                                                                                                                                                    aGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGAca  <  1:95650/151‑1 (MQ=255)

AAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACA  >  NC_000913/3587382‑3587680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`