Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913306,0230GA54.5% 117.1 / inf 420T265T (ACC→ACT) ecpDpolymerized tip adhesin of ECP fibers
Reads supporting (aligned to +/- strand):  ref base G (91/99);  new base A (109/119);  total (200/220)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 429 total aligned reads displayed.

GTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGTAGATTTGCT  >  NC_000913/305873‑306173
                                                                                                                                                      |                                                                                                                                                      
  agtaATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAggg                                                                                                                                                       <  8:116230/150‑1 (MQ=255)
    taATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGGAGAGtat                                                                                                                                                    >  7:1224356/1‑151 (MQ=255)
      aTAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGtatat                                                                                                                                                  >  2:432835/1‑150 (MQ=255)
      aTAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGtat                                                                                                                                                    >  4:1600094/1‑149 (MQ=255)
        aGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGtt                                                                                                                                                >  1:1631906/1‑151 (MQ=255)
        aGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGtt                                                                                                                                                >  4:813892/1‑151 (MQ=255)
         ggCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGtt                                                                                                                                                >  7:1592313/1‑150 (MQ=255)
           cGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCGGACGGCCGAGGCTAACGACGAGTTGGCGATGGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAAc                                                                                                                                             <  6:609463/151‑1 (MQ=255)
            ggTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAAcc                                                                                                                                            <  7:1486565/151‑1 (MQ=255)
            ggTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAAcc                                                                                                                                            >  8:1035565/1‑151 (MQ=255)
             gTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAAcc                                                                                                                                            >  7:542784/1‑150 (MQ=255)
               aTTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATc                                                                                                                                         >  2:1114856/1‑151 (MQ=255)
                ttGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCt                                                                                                                                        >  8:385610/1‑151 (MQ=255)
                 tGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGGAGGGTATAGTTAACCATCtt                                                                                                                                       >  4:1168160/1‑151 (MQ=255)
                   cTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTAc                                                                                                                                     >  3:986500/1‑151 (MQ=255)
                     cACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACaa                                                                                                                                   <  3:1703735/151‑1 (MQ=255)
                         ggTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTa                                                                                                                               <  6:1567405/151‑1 (MQ=255)
                         ggTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACtt                                                                                                                                >  3:465548/1‑150 (MQ=255)
                         gctttCCATGAACTGCCATTCAGACTCAACTTCATATCGTACGCCCCGACGTCCGACGCTAACGACGACTTCGCGTTCCCCGGGAATAAATGGATTGAGGTGTTGCTGAGTCAGTTTGTATGCAGAGTATAGTTAACCATCTTACAACtt                                                                                                                                >  2:32308/3‑150 (MQ=255)
                          gTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTaa                                                                                                                              <  5:354663/151‑1 (MQ=255)
                            ttCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGCGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTAGAACTTAATc                                                                                                                            >  2:65939/1‑151 (MQ=255)
                            ttCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATc                                                                                                                            >  7:589935/1‑151 (MQ=255)
                            ttCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATc                                                                                                                            >  1:848765/1‑151 (MQ=255)
                             tCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATc                                                                                                                            >  4:1048850/1‑150 (MQ=255)
                              ccATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCt                                                                                                                          <  8:766269/151‑1 (MQ=255)
                              ccATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCt                                                                                                                          <  4:1310209/151‑1 (MQ=255)
                               cATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTg                                                                                                                         >  8:1670422/1‑151 (MQ=255)
                                       gCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCt                                                                                                                  <  7:97794/150‑1 (MQ=255)
                                       cccATCCAGACTGAACTGCATATCGGACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCACAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTg                                                                                                                 <  6:1720918/150‑1 (MQ=255)
                                          aTTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCg                                                                                                              >  1:939000/1‑151 (MQ=255)
                                           ttCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCg                                                                                                              >  1:1537557/1‑150 (MQ=255)
                                             cAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGAt                                                                                                            <  1:1180112/150‑1 (MQ=255)
                                              aGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATgg                                                                                                          >  7:1285157/1‑151 (MQ=255)
                                               gACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGt                                                                                                         <  5:1152665/151‑1 (MQ=255)
                                                    aaCTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTTACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCg                                                                                                    >  5:1320829/1‑151 (MQ=255)
                                                    aaCTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCg                                                                                                    <  5:1084429/151‑1 (MQ=255)
                                                       tGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTcc                                                                                                 >  5:824045/1‑151 (MQ=255)
                                                        gCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTc                                                                                                  <  8:1575763/149‑1 (MQ=255)
                                                        gCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCg                                                                                                <  1:495553/151‑1 (MQ=255)
                                                        gCATAGCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCg                                                                                                <  2:917940/151‑1 (MQ=255)
                                                          atatCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGc                                                                                              >  8:474115/1‑151 (MQ=255)
                                                          atatCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGc                                                                                              >  2:1166440/1‑151 (MQ=255)
                                                            atCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAg                                                                                            <  3:88438/151‑1 (MQ=255)
                                                               gTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCg                                                                                         <  7:753657/151‑1 (MQ=255)
                                                               gTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCg                                                                                         >  4:1507377/1‑151 (MQ=255)
                                                                           ggCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCg                                                                             >  7:1074245/1‑151 (MQ=255)
                                                                               gAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGccc                                                                          >  6:145630/1‑150 (MQ=255)
                                                                                 ggCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCGGTTAGTTTGCAGAGTATAGTTAACCTTCTTACAAGTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCaga                                                                       <  4:37477/151‑1 (MQ=255)
                                                                                  gCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCag                                                                        >  7:869353/1‑149 (MQ=255)
                                                                                    tAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTc                                                                    >  3:599482/1‑151 (MQ=255)
                                                                                       cgacgaGTGGGCGATCGCCGGGAATAGATGGATTGAGGGGTTGCTGAGTCGGTTTGTGTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGt                                                                 <  6:516770/151‑1 (MQ=255)
                                                                                         acgaGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTAc                                                               <  4:19352/151‑1 (MQ=255)
                                                                                             gTTGGCGATCGCCGGGAATATATGGATTGAGGTGGTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCg                                                           <  3:269714/151‑1 (MQ=255)
                                                                                                ggCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCg                                                        <  4:951941/151‑1 (MQ=255)
                                                                                                  cGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCt                                                      <  4:730125/151‑1 (MQ=255)
                                                                                                   gATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTg                                                     >  3:1635867/1‑151 (MQ=255)
                                                                                                      cGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTg                                                  <  3:684255/151‑1 (MQ=255)
                                                                                                      cGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTg                                                  >  3:1366144/1‑151 (MQ=255)
                                                                                                       gCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTg                                                  >  4:307497/1‑150 (MQ=255)
                                                                                                       gCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTg                                                  >  8:122275/1‑150 (MQ=255)
                                                                                                       gCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTCCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTg                                                  >  8:671042/1‑150 (MQ=255)
                                                                                                         cGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGAt                                                >  2:1685497/1‑150 (MQ=255)
                                                                                                          gggAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATaa                                              <  5:1688911/151‑1 (MQ=255)
                                                                                                          gggAATATATGGATTGAGGTGTTGCTGAGACCGTTTGTTTGCAGCGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATaa                                              <  6:1334025/151‑1 (MQ=255)
                                                                                                           ggAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATaa                                              >  2:130061/1‑150 (MQ=255)
                                                                                                            gAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAAt                                            <  4:68068/151‑1 (MQ=255)
                                                                                                            gAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAAt                                            >  1:199788/1‑151 (MQ=255)
                                                                                                             aaTATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATa                                           <  8:880365/151‑1 (MQ=255)
                                                                                                                atatGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACtt                                        >  5:796355/1‑151 (MQ=255)
                                                                                                                atatGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACtt                                        <  7:391427/151‑1 (MQ=255)
                                                                                                                atatGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACtt                                        <  8:1470214/151‑1 (MQ=255)
                                                                                                                atatCGATTGCGGGGTCGCGGACTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGCACGGCTGCCGATGGTTTGCGGCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACtt                                        <  4:493036/151‑1 (MQ=255)
                                                                                                                  atGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTcc                                      >  2:1241297/1‑151 (MQ=255)
                                                                                                                     gATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGcc                                   >  4:907620/1‑151 (MQ=255)
                                                                                                                      aTTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAAGCATCTTACAACTTAATCCTGAACGCCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCa                                  <  2:497800/151‑1 (MQ=255)
                                                                                                                      aTTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGcc                                   >  6:978980/1‑150 (MQ=255)
                                                                                                                        tGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAg                                 <  6:1228715/150‑1 (MQ=255)
                                                                                                                          aGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGa                              <  1:1035084/151‑1 (MQ=255)
                                                                                                                           ggTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAg                             <  5:1183590/151‑1 (MQ=255)
                                                                                                                                tGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTggg                         <  7:180951/150‑1 (MQ=255)
                                                                                                                                tGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTggg                         <  5:489698/150‑1 (MQ=255)
                                                                                                                                tGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGt                        >  1:1390773/1‑151 (MQ=255)
                                                                                                                                  cTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTAt                      >  2:1446983/1‑151 (MQ=255)
                                                                                                                                    gAGGCCGTTTGTTTGCAGGGTATAGTAAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTAtt                     <  7:1384685/150‑1 (MQ=255)
                                                                                                                                     cggccGTTTGGTTGCAGAGTATAGTTCACCATCTTCCAACTTAATCCTGAACGGCTGCCGATGGTCTGCGTCCGGCAGTCGGCGTTCCCTTCCCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTa                   <  6:1098459/148‑1 (MQ=255)
                                                                                                                                      gTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGGGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTAt                  <  2:1025825/151‑1 (MQ=255)
                                                                                                                                        ccGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATc                 <  1:278887/150‑1 (MQ=255)
                                                                                                                                        ccGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCa                <  8:25327/151‑1 (MQ=255)
                                                                                                                                            ttgtttgCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCg            >  5:1581738/1‑151 (MQ=255)
                                                                                                                                            ttgtttgCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTCTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCg            >  1:585401/1‑151 (MQ=255)
                                                                                                                                            ttgtttgCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAAcc             <  4:610011/150‑1 (MQ=255)
                                                                                                                                             tgtttgCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCg            <  2:765202/150‑1 (MQ=255)
                                                                                                                                             tgtttgCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAAAGGCTGCCGATGGTTTGCGTCAGGCAGTCGGCGTTCCCTTCGCCCAGAGTAGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGt           >  5:956010/1‑151 (MQ=255)
                                                                                                                                              gtttgCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGt           >  1:712589/1‑150 (MQ=255)
                                                                                                                                              gtttgCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGTa          <  5:1473758/151‑1 (MQ=255)
                                                                                                                                                ttgCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGt           >  6:694011/1‑148 (MQ=255)
                                                                                                                                                  gCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGTAGAt       <  1:413289/150‑1 (MQ=255)
                                                                                                                                                   cAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGTAGAttt     >  1:1598710/1‑151 (MQ=255)
                                                                                                                                                   cAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGTAGAttt     >  2:826920/1‑151 (MQ=255)
                                                                                                                                                   cAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGTAGAttt     >  6:345593/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
GTAGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGTAGATTTGCT  >  NC_000913/305873‑306173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑